P P Provenzano

Summary

Affiliation: Fred Hutchinson Cancer Research Center
Country: USA

Publications

  1. pmc Mechanical signaling through the cytoskeleton regulates cell proliferation by coordinated focal adhesion and Rho GTPase signaling
    Paolo P Provenzano
    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    J Cell Sci 124:1195-205. 2011
  2. pmc Matrix density-induced mechanoregulation of breast cell phenotype, signaling and gene expression through a FAK-ERK linkage
    P P Provenzano
    Department of Pharmacology, University of Wisconsin, Madison, WI, USA
    Oncogene 28:4326-43. 2009
  3. pmc Shining new light on 3D cell motility and the metastatic process
    Paolo P Provenzano
    Laboratory for Optical and Computational Instrumentation, and Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA
    Trends Cell Biol 19:638-48. 2009
  4. pmc The role of focal adhesion kinase in tumor initiation and progression
    Paolo P Provenzano
    Laboratory of Molecular Biology and Departments of Pharmacology and Biomedical Engineering, University of Wisconsin, Madison, WI, USA
    Cell Adh Migr 3:347-50. 2009
  5. doi Nonlinear optical microscopy and computational analysis of intrinsic signatures in breast cancer
    Curtis T Rueden
    Laboratory for Optical and Computational Instrumentation, University of Wisconsin, Madison, WI 53706 USA
    Conf Proc IEEE Eng Med Biol Soc 2009:4077-80. 2009
  6. pmc Contact guidance mediated three-dimensional cell migration is regulated by Rho/ROCK-dependent matrix reorganization
    Paolo P Provenzano
    Department of Pharmacology, Laboratory of Molecular Biology, University of Wisconsin Paul P Carbone Comprehensive Cancer Center, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biophys J 95:5374-84. 2008
  7. pmc Engineering three-dimensional collagen matrices to provide contact guidance during 3D cell migration
    Paolo P Provenzano
    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    Curr Protoc Cell Biol . 2010
  8. doi Multiphoton microscopy and fluorescence lifetime imaging microscopy (FLIM) to monitor metastasis and the tumor microenvironment
    Paolo P Provenzano
    Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
    Clin Exp Metastasis 26:357-70. 2009
  9. doi Fluorescence lifetime imaging of endogenous fluorophores in histopathology sections reveals differences between normal and tumor epithelium in carcinoma in situ of the breast
    Matthew W Conklin
    Department of Pharmacology, University of Wisconsin, 1525 Linden Dr, Madison, WI 53706, USA
    Cell Biochem Biophys 53:145-57. 2009
  10. pmc Mammary epithelial-specific disruption of focal adhesion kinase retards tumor formation and metastasis in a transgenic mouse model of human breast cancer
    Paolo P Provenzano
    Department of Pharmacology, Laboratory of Molecular Biology, Madison, WI 53706, USA ppproven wisc edu
    Am J Pathol 173:1551-65. 2008

Collaborators

Detail Information

Publications13

  1. pmc Mechanical signaling through the cytoskeleton regulates cell proliferation by coordinated focal adhesion and Rho GTPase signaling
    Paolo P Provenzano
    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    J Cell Sci 124:1195-205. 2011
    ..Moreover, as there is much recent interest in the tumor-stromal interaction, we will pay particular attention to exciting new data describing the role of mechanical signaling in the progression of breast cancer...
  2. pmc Matrix density-induced mechanoregulation of breast cell phenotype, signaling and gene expression through a FAK-ERK linkage
    P P Provenzano
    Department of Pharmacology, University of Wisconsin, Madison, WI, USA
    Oncogene 28:4326-43. 2009
    ..As such, we propose that increased matrix stiffness explains part of the mechanism behind increased epithelial proliferation and cancer risk in human patients with high breast tissue density...
  3. pmc Shining new light on 3D cell motility and the metastatic process
    Paolo P Provenzano
    Laboratory for Optical and Computational Instrumentation, and Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA
    Trends Cell Biol 19:638-48. 2009
    ....
  4. pmc The role of focal adhesion kinase in tumor initiation and progression
    Paolo P Provenzano
    Laboratory of Molecular Biology and Departments of Pharmacology and Biomedical Engineering, University of Wisconsin, Madison, WI, USA
    Cell Adh Migr 3:347-50. 2009
    ..Hence, FAK, as well as the FAK-regulating/regulated signaling network, are viable candidates for cancer metastasis therapies...
  5. doi Nonlinear optical microscopy and computational analysis of intrinsic signatures in breast cancer
    Curtis T Rueden
    Laboratory for Optical and Computational Instrumentation, University of Wisconsin, Madison, WI 53706 USA
    Conf Proc IEEE Eng Med Biol Soc 2009:4077-80. 2009
    ..In particular we present the latest development in our SLIM Plotter application, an open source visualization program for interactive visualization and inspection of combined spectral lifetime (SLIM) data...
  6. pmc Contact guidance mediated three-dimensional cell migration is regulated by Rho/ROCK-dependent matrix reorganization
    Paolo P Provenzano
    Department of Pharmacology, Laboratory of Molecular Biology, University of Wisconsin Paul P Carbone Comprehensive Cancer Center, University of Wisconsin, Madison, Wisconsin 53706, USA
    Biophys J 95:5374-84. 2008
    ....
  7. pmc Engineering three-dimensional collagen matrices to provide contact guidance during 3D cell migration
    Paolo P Provenzano
    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    Curr Protoc Cell Biol . 2010
    ..These assays are readily adaptable to a variety of cells and biological questions related to three-dimensional cell migration...
  8. doi Multiphoton microscopy and fluorescence lifetime imaging microscopy (FLIM) to monitor metastasis and the tumor microenvironment
    Paolo P Provenzano
    Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
    Clin Exp Metastasis 26:357-70. 2009
    ..Herein, we review recent developments of multiphoton FLIM and SLIM to study 2D and 3D cell migration, invasion into the tumor microenvironment, and metastasis...
  9. doi Fluorescence lifetime imaging of endogenous fluorophores in histopathology sections reveals differences between normal and tumor epithelium in carcinoma in situ of the breast
    Matthew W Conklin
    Department of Pharmacology, University of Wisconsin, 1525 Linden Dr, Madison, WI 53706, USA
    Cell Biochem Biophys 53:145-57. 2009
    ..Most forms of cancer have altered metabolism and redox ratios; here we present a method that has potential for early detection of these changes, which are preserved in fixed tissue samples such as classic histopathology slides...
  10. pmc Mammary epithelial-specific disruption of focal adhesion kinase retards tumor formation and metastasis in a transgenic mouse model of human breast cancer
    Paolo P Provenzano
    Department of Pharmacology, Laboratory of Molecular Biology, Madison, WI 53706, USA ppproven wisc edu
    Am J Pathol 173:1551-65. 2008
    ..Therefore, FAK acts as a primary node in the activated signaling network in transformed motile cells and is a prime candidate for novel therapeutic interventions to treat aggressive human breast cancers...
  11. pmc Collagen density promotes mammary tumor initiation and progression
    Paolo P Provenzano
    Department of Pharmacology, University of Wisconsin, Madison, WI 53706, USA
    BMC Med 6:11. 2008
    ..Importantly, regions of high breast density are associated with increased stromal collagen. Thus, the influence of the extracellular matrix on breast carcinoma development and the underlying molecular mechanisms are not understood...
  12. ncbi Investigating integrin regulation and signaling events in three-dimensional systems
    Patricia J Keely
    Department of Pharmacology, University of Wisconsin Medical School, University of Wisconsin Madison, Madison, Wisconsin, USA
    Methods Enzymol 426:27-45. 2007
    ..The purpose of this chapter is to provide a few overall strategies for working within 3D culture systems, focusing on biochemical and molecular imaging approaches...
  13. pmc Collagen reorganization at the tumor-stromal interface facilitates local invasion
    Paolo P Provenzano
    Department of Pharmacology, University of Wisconsin, Madison, WI, USA
    BMC Med 4:38. 2006
    ....