- Neurobeachin is required postsynaptically for electrical and chemical synapse formationAdam C Miller
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, Seattle, WA 98109, USA Electronic address
Curr Biol 25:16-28. 2015..The molecular pathways required for electrical synaptogenesis are not well understood, and whether they share mechanisms of formation with chemical synapses is not clear...
- RNA-seq-based mapping and candidate identification of mutations from forward genetic screensAdam C Miller
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Genome Res 23:679-86. 2013..Overall, we show that RNA-seq is a fast, reliable, and cost-effective method to map and identify mutations that will greatly facilitate the power of forward genetics in vertebrate models...
- Rapid reverse genetic screening using CRISPR in zebrafishArish N Shah
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
Nat Methods 12:535-40. 2015..By deep sequencing target loci, we found that 90% of the genes were effectively screened. We conclude that CRISPR can be used as a powerful reverse genetic screening strategy in vivo in a vertebrate system. ..