Paul Lampe

Summary

Affiliation: Fred Hutchinson Cancer Research Center
Country: USA

Publications

  1. pmc Cruciferous vegetable supplementation in a controlled diet study alters the serum peptidome in a GSTM1-genotype dependent manner
    Heather Ann Brauer
    Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98195, USA
    Nutr J 10:11. 2011
  2. pmc Use of cancer-specific yeast-secreted in vivo biotinylated recombinant antibodies for serum biomarker discovery
    Nathalie Scholler
    Center for Research on Early Detection and Cure of Ovarian Cancer, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Transl Med 6:41. 2008
  3. pmc Analysis of Connexin43 phosphorylated at S325, S328 and S330 in normoxic and ischemic heart
    Paul D Lampe
    Molecular Diagnostics Program, Fred Hutchinson Cancer Research Center, Department of Pathobiology, University of Washington, 1100 Fairview Avenue N, M5C800, PO Box 19024, Seattle, WA 98109, USA
    J Cell Sci 119:3435-42. 2006
  4. pmc Cellular interaction of integrin alpha3beta1 with laminin 5 promotes gap junctional communication
    P D Lampe
    Divisions of Basic Sciences and Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    J Cell Biol 143:1735-47. 1998
  5. pmc The effects of connexin phosphorylation on gap junctional communication
    Paul D Lampe
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North DE 320, Box 19024, Seattle, WA 98109, USA
    Int J Biochem Cell Biol 36:1171-86. 2004
  6. pmc Phosphorylation of connexin43 on serine368 by protein kinase C regulates gap junctional communication
    P D Lampe
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    J Cell Biol 149:1503-12. 2000
  7. ncbi request reprint Gap junction assembly: PTX-sensitive G proteins regulate the distribution of connexin43 within cells
    P D Lampe
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Am J Physiol Cell Physiol 281:C1211-22. 2001
  8. pmc Altered tumor biology and tumorigenesis in irradiated and chemical carcinogen-treated single and combined connexin32/p27Kip1-deficient mice
    Timothy J King
    Molecular Diagnostics Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    Cell Commun Adhes 12:293-305. 2005
  9. pmc Temporal regulation of connexin phosphorylation in embryonic and adult tissues
    Timothy J King
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M5C800, Box 19024, Seattle, WA 98109, USA
    Biochim Biophys Acta 1719:24-35. 2005
  10. ncbi request reprint Quantifying peptide signal in MALDI-TOF mass spectrometry data
    Timothy W Randolph
    Department of Biostatistics, University of Washington, Seattle, Washington 98195, USA
    Mol Cell Proteomics 4:1990-9. 2005

Research Grants

  1. PHOSPHORYLATION OF GAP JUNCTION PROTEINS
    Paul D Lampe; Fiscal Year: 2010
  2. PHOSPHORYLATION OF GAP JUNCTION PROTEINS
    Paul Lampe; Fiscal Year: 2007
  3. Proteomic Biomarkers of Health Behaviors
    Paul Lampe; Fiscal Year: 2005
  4. PHOSPHORYLATION OF GAP JUNCTION PROTEINS
    Paul Lampe; Fiscal Year: 2005
  5. PHOSPHORYLATION OF GAP JUNCTION PROTEINS
    Paul Lampe; Fiscal Year: 2000
  6. PHOSPHORYLATION OF GAP JUNCTION PROTEINS
    Paul Lampe; Fiscal Year: 2009

Collaborators

Detail Information

Publications38

  1. pmc Cruciferous vegetable supplementation in a controlled diet study alters the serum peptidome in a GSTM1-genotype dependent manner
    Heather Ann Brauer
    Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98195, USA
    Nutr J 10:11. 2011
    ..The polymorphic glutathione-S-transferase (GST) gene family encodes several enzymes which catalyze ITC degradation in vivo...
  2. pmc Use of cancer-specific yeast-secreted in vivo biotinylated recombinant antibodies for serum biomarker discovery
    Nathalie Scholler
    Center for Research on Early Detection and Cure of Ovarian Cancer, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Transl Med 6:41. 2008
    ..We developed a novel technology that permits us to isolate recombinant antibodies directed against the potential serum biomarkers, to facilitate the further development of affinity reagents necessary to construct diagnostic tests...
  3. pmc Analysis of Connexin43 phosphorylated at S325, S328 and S330 in normoxic and ischemic heart
    Paul D Lampe
    Molecular Diagnostics Program, Fred Hutchinson Cancer Research Center, Department of Pathobiology, University of Washington, 1100 Fairview Avenue N, M5C800, PO Box 19024, Seattle, WA 98109, USA
    J Cell Sci 119:3435-42. 2006
    ..These data suggest that phosphorylation of Cx43 at serine residues 325, 328 and/or 330 influences channel permselectivity and regulates the efficiency of gap junction assembly...
  4. pmc Cellular interaction of integrin alpha3beta1 with laminin 5 promotes gap junctional communication
    P D Lampe
    Divisions of Basic Sciences and Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    J Cell Biol 143:1735-47. 1998
    ..We suggest that adhesion of epithelial cells to laminin 5 in the basement membrane via alpha3beta1 promotes GJIC that integrates individual cells into synchronized epiboles...
  5. pmc The effects of connexin phosphorylation on gap junctional communication
    Paul D Lampe
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North DE 320, Box 19024, Seattle, WA 98109, USA
    Int J Biochem Cell Biol 36:1171-86. 2004
    ..In several cases, use of site-directed mutants of these sites have shown that these specific phosphorylation events can be linked to changes in gap junctional communication...
  6. pmc Phosphorylation of connexin43 on serine368 by protein kinase C regulates gap junctional communication
    P D Lampe
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    J Cell Biol 149:1503-12. 2000
    ..These data strongly suggest that PKC directly phosphorylates Cx43 on S368 in vivo, which results in a change in single channel behavior that contributes to a decrease in intercellular communication...
  7. ncbi request reprint Gap junction assembly: PTX-sensitive G proteins regulate the distribution of connexin43 within cells
    P D Lampe
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Am J Physiol Cell Physiol 281:C1211-22. 2001
    ..These results suggest that PTX-sensitive G proteins regulate connexin43 trafficking, and, as a result of inhibition with PTX, the number of plasma membrane hemichannels available for GJ assembly is reduced...
  8. pmc Altered tumor biology and tumorigenesis in irradiated and chemical carcinogen-treated single and combined connexin32/p27Kip1-deficient mice
    Timothy J King
    Molecular Diagnostics Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    Cell Commun Adhes 12:293-305. 2005
    ..Furthermore, these studies demonstrate that different carcinogens interact disparately with Cx32/p27Kip1 genotypic backgrounds in situ resulting in varied tumorigenic response...
  9. pmc Temporal regulation of connexin phosphorylation in embryonic and adult tissues
    Timothy J King
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M5C800, Box 19024, Seattle, WA 98109, USA
    Biochim Biophys Acta 1719:24-35. 2005
    ..These new antibody tools and those under development will allow us to correlate specific phosphorylation events with changes in connexin function...
  10. ncbi request reprint Quantifying peptide signal in MALDI-TOF mass spectrometry data
    Timothy W Randolph
    Department of Biostatistics, University of Washington, Seattle, Washington 98195, USA
    Mol Cell Proteomics 4:1990-9. 2005
    ....
  11. ncbi request reprint Effect of a 12-month exercise intervention on patterns of cellular proliferation in colonic crypts: a randomized controlled trial
    Anne McTiernan
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M4 B402, Seattle, WA 98109 1024, USA
    Cancer Epidemiol Biomarkers Prev 15:1588-97. 2006
    ..A hypothesized mechanism for exercise-induced colon cancer risk reduction might be through alterations in colon crypt cell architecture and proliferation...
  12. ncbi request reprint Exercise effect on weight and body fat in men and women
    Anne McTiernan
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, M4 B402, Seattle, WA 98109 1024, USA
    Obesity (Silver Spring) 15:1496-512. 2007
    ..The effect of national exercise recommendations on adiposity is unknown and may differ by sex. We examined long-term effects of aerobic exercise on adiposity in women and men...
  13. pmc Key connexin 43 phosphorylation events regulate the gap junction life cycle
    Joell L Solan
    Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    J Membr Biol 217:35-41. 2007
    ..However, phosphorylation at S262 could explain the shift. By defining these phosphorylation events, we have begun to sort out the critical signaling pathways that regulate gap junction function...
  14. pmc Phosphorylation at S365 is a gatekeeper event that changes the structure of Cx43 and prevents down-regulation by PKC
    Joell L Solan
    Molecular Diagnostics Program and 2Proteomics Shared Resource, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    J Cell Biol 179:1301-9. 2007
    ..Thus, phosphorylation at S365 can serve a "gatekeeper" function that may represent a mechanism to protect cells from ischemia and phorbol ester-induced down-regulation of channel conductance...
  15. ncbi request reprint Risk factors for colorectal cancer in relation to number and size of aberrant crypt foci in humans
    Rebecca E Rudolph
    Veterans Affairs Puget Sound Health Care System, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    Cancer Epidemiol Biomarkers Prev 14:605-8. 2005
    ..Future research should be directed toward developing techniques to identify ACF that are likely to progress to cancer and the modifiable factors that promote or inhibit such progression...
  16. ncbi request reprint Deficiency in the gap junction protein connexin32 alters p27Kip1 tumor suppression and MAPK activation in a tissue-specific manner
    Timothy J King
    Cancer Prevention Research Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Oncogene 24:1718-26. 2005
    ..This study demonstrates that tissues dependent on Cx32 tumor suppression, such as the liver and lung, exhibit altered tumorigenesis and tumor biology (MAPK pathway activation) related to p27 status...
  17. ncbi request reprint The gap junction protein connexin32 is a mouse lung tumor suppressor
    Timothy J King
    Cancer Prevention Research Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, and Department of Pathobiology, University of Washington, Seattle, Washington, USA
    Cancer Res 64:7191-6. 2004
    ..001) compared with wild-type tumors, implicating this signaling pathway in Cx32/gap junction intercellular communication-associated lung tumorigenesis...
  18. ncbi request reprint Connexin43 phosphorylation at S368 is acute during S and G2/M and in response to protein kinase C activation
    Joell L Solan
    Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    J Cell Sci 116:2203-11. 2003
    ..Thus, both gap junctional communication and S368 phosphorylation change during S phase and G2/M, implying that phosphorylation at S368 might play a role in key cell-cycle events...
  19. ncbi request reprint Mice deficient for the gap junction protein Connexin32 exhibit increased radiation-induced tumorigenesis associated with elevated mitogen-activated protein kinase (p44/Erk1, p42/Erk2) activation
    Timothy J King
    Cancer Prevention Research Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Carcinogenesis 25:669-80. 2004
    ....
  20. ncbi request reprint New developments in the gap junction field: report from the 2002 ASCB meeting
    Paul Lampe
    Department of Pathobiology, Fred Hutchinson Cancer Research Center, Seattle, WA, USA
    Cell Commun Adhes 10:55-8. 2003
  21. ncbi request reprint Casein kinase 1 regulates connexin-43 gap junction assembly
    Cynthia D Cooper
    Fred Hutchinson Cancer Research Center and Department of Pathobiology, University of Washington, Seattle, Washington 98109 1024, USA
    J Biol Chem 277:44962-8. 2002
    ..These data suggest CK1delta could regulate Cx43 gap junction assembly by directly phosphorylating Cx43...
  22. pmc Connexin 43 in LA-25 cells with active v-src is phosphorylated on Y247, Y265, S262, S279/282, and S368 via multiple signaling pathways
    Joell L Solan
    Molecular Diagnostics Program, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Cell Commun Adhes 15:75-84. 2008
    ..These results indicate all three signaling pathways could contribute to gap junction down-regulation during src transformation in LA-25 cells...
  23. ncbi request reprint Connexin phosphorylation as a regulatory event linked to gap junction channel assembly
    Joell L Solan
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M5C800, Box 19024, Seattle, Washington 98109, United States
    Biochim Biophys Acta 1711:154-63. 2005
    ....
  24. pmc Protein kinase C spatially and temporally regulates gap junctional communication during human wound repair via phosphorylation of connexin43 on serine368
    Theresa S Richards
    Division of Basic and Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    J Cell Biol 167:555-62. 2004
    ..Our evidence indicates that PKC-dependent phosphorylation of Cx43 at S368 creates dynamic communication compartments that can temporally and spatially regulate wound healing...
  25. pmc Connexin43 phosphorylation: structural changes and biological effects
    Joell L Solan
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M5C800 Box 19024, Seattle, WA 98109, USA
    Biochem J 419:261-72. 2009
    ..The present review assesses our current understanding of the effects of phosphorylation on connexin43 structure and function that in turn regulate gap junction biology, with an emphasis on events occurring in heart and skin...
  26. ncbi request reprint Inducible expression of the gap junction protein connexin43 decreases the neoplastic potential of HT-1080 human fibrosarcoma cells in vitro and in vivo
    Timothy J King
    Cancer Prevention Research Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Mol Carcinog 35:29-41. 2002
    ..This study also further supports the hypothesis that loss of Cx43/GJIC in human tumors may play an important role in the dysregulation of normal growth control...
  27. ncbi request reprint Soy protein containing isoflavones does not decrease colorectal epithelial cell proliferation in a randomized controlled trial
    Kenneth F Adams
    Fred Hutchinson Cancer Research Center, Seattle, WA, USA
    Am J Clin Nutr 82:620-6. 2005
    ..Colorectal epithelial cell proliferation has been used extensively as an intermediate endpoint biomarker for colorectal neoplasia...
  28. ncbi request reprint Evaluation of matrix-assisted laser desorption/ionization-time of flight mass spectrometry proteomic profiling: identification of alpha 2-HS glycoprotein B-chain as a biomarker of diet
    Breeana L Mitchell
    Fred Hutchinson Cancer Research Center, University of Washington, Seattle, 98109, USA
    Proteomics 5:2238-46. 2005
    ..cruciferous) with 76% accuracy. The 2740 m/z peak was identified as the B-chain of alpha 2-HS glycoprotein, a serum protein previously found to vary with diet and be involved in insulin resistance and immune function...
  29. pmc Connexin 43 interacts with zona occludens-1 and -2 proteins in a cell cycle stage-specific manner
    Deepika Singh
    Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    J Biol Chem 280:30416-21. 2005
    ..Since essentially all of the Cx43 in G0 cells is assembled into Triton X-100-resistant junctions, Cx43-ZO-1 interaction may contribute to their stability...
  30. ncbi request reprint Effect of a 12-month exercise intervention on the apoptotic regulating proteins Bax and Bcl-2 in colon crypts: a randomized controlled trial
    Kristin L Campbell
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M4 B402, Seattle, WA 98109 1024, USA
    Cancer Epidemiol Biomarkers Prev 16:1767-74. 2007
    ..The objective of this study is to examine the effects of a 12-month aerobic exercise program on expression of proteins that promote (Bax) or inhibit (Bcl-2) apoptosis in colon crypts...
  31. pmc Methodologies for characterizing phosphoproteins by mass spectrometry
    Philip R Gafken
    Proteomics Facility, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Cell Commun Adhes 13:249-62. 2006
    ....
  32. ncbi request reprint Identification of connexin-43 interacting proteins
    Deepika Singh
    Fred Hutchinson Cancer Research Center and Pathobiology Department, University of Washington, Seattle, WA 98109, USA
    Cell Commun Adhes 10:215-20. 2003
    ..Further in vitro/in vivo analysis of these interacting proteins will help in our understanding of the global role of connexins in regulating development, cell metabolism and growth...
  33. ncbi request reprint No effect of exercise on colon mucosal prostaglandin concentrations: a 12-month randomized controlled trial
    Page E Abrahamson
    Cancer Prevention, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    Cancer Epidemiol Biomarkers Prev 16:2351-6. 2007
    ..We hypothesized that an exercise intervention would decrease prostaglandin E(2) concentrations and increase prostaglandin F(2alpha) in colon biopsies compared with controls...
  34. pmc Gap junction remodeling and cardiac arrhythmogenesis in a murine model of oculodentodigital dysplasia
    Nellie Kalcheva
    Leon H Charney Division of Cardiology, New York University School of Medicine, New York, NY 10016, USA
    Proc Natl Acad Sci U S A 104:20512-6. 2007
    ..In summary, our data demonstrate that the I130T mutation interferes with Cx43 posttranslational processing, resulting in diminished cell-cell coupling, slowing of impulse propagation, and a proarrhythmic substrate...
  35. pmc The C-terminus of connexin43 adopts different conformations in the Golgi and gap junction as detected with structure-specific antibodies
    Gina E Sosinsky
    National Center for Microscopy and Imaging Research, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA 92093 0608, USA
    Biochem J 408:375-85. 2007
    ..These studies indicate that the conformation of Ser364/Ser365 is important for intracellular localization, whereas the tertiary structure of Pro375-Asp379 is essential in targeting and regulation of gap junctional connexin43...
  36. pmc Selectivity of connexin 43 channels is regulated through protein kinase C-dependent phosphorylation
    Jose F Ek-Vitorin
    Department of Physiology, University of Arizona, Tucson 85724, USA
    Circ Res 98:1498-505. 2006
    ..Our data suggest that the selectivity of gap junction channels at intercalated disks is increased early in ischemia...
  37. ncbi request reprint Gap junctions between cells expressing connexin 43 or 32 show inverse permselectivity to adenosine and ATP
    Gary S Goldberg
    Department of Physiology and Biophysics, State University of New York, Stony Brook, New York 11794 8661, USA
    J Biol Chem 277:36725-30. 2002
    ..This suggests functional consequence because the energy status of a cell could be controlled via connexin expression and channel formation...
  38. ncbi request reprint Enhanced myocyte contractility and Ca2+ handling in a calcineurin transgenic model of heart failure
    Guoxiang Chu
    Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, 231 Albert B Sabin Way, OH 45267 0575, USA
    Cardiovasc Res 54:105-16. 2002
    ..We examined whether defects in cardiomyocyte Ca2+ handling properties contribute to the impaired cardiac function in calcineurin transgenic mice...

Research Grants14

  1. PHOSPHORYLATION OF GAP JUNCTION PROTEINS
    Paul D Lampe; Fiscal Year: 2010
    ....
  2. PHOSPHORYLATION OF GAP JUNCTION PROTEINS
    Paul Lampe; Fiscal Year: 2007
    ..We hypothesize that Cx43 phosphorylation changes are linked to these processes in a way that affects cellular function. ..
  3. Proteomic Biomarkers of Health Behaviors
    Paul Lampe; Fiscal Year: 2005
    ..abstract_text> ..
  4. PHOSPHORYLATION OF GAP JUNCTION PROTEINS
    Paul Lampe; Fiscal Year: 2005
    ..We plan to apply our knowledge of connexin phosphorylation and phosphorylation specific probes to the analysis of connexin status in wounded skin and the remodeling of gap junctions during heart disease. ..
  5. PHOSPHORYLATION OF GAP JUNCTION PROTEINS
    Paul Lampe; Fiscal Year: 2000
    ....
  6. PHOSPHORYLATION OF GAP JUNCTION PROTEINS
    Paul Lampe; Fiscal Year: 2009
    ..We hypothesize that Cx43 phosphorylation changes are linked to these processes in a way that affects cellular function. ..