Research Topics
Genomes and Genes | Paul LampeSummaryAffiliation: Fred Hutchinson Cancer Research Center Country: USA Publications
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Publications
Cruciferous vegetable supplementation in a controlled diet study alters the serum peptidome in a GSTM1-genotype dependent mannerHeather Ann Brauer
Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98195, USA
Nutr J 10:11. 2011..The polymorphic glutathione-S-transferase (GST) gene family encodes several enzymes which catalyze ITC degradation in vivo...- Use of cancer-specific yeast-secreted in vivo biotinylated recombinant antibodies for serum biomarker discoveryNathalie Scholler
Center for Research on Early Detection and Cure of Ovarian Cancer, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
J Transl Med 6:41. 2008..We developed a novel technology that permits us to isolate recombinant antibodies directed against the potential serum biomarkers, to facilitate the further development of affinity reagents necessary to construct diagnostic tests... - Analysis of Connexin43 phosphorylated at S325, S328 and S330 in normoxic and ischemic heartPaul D Lampe
Molecular Diagnostics Program, Fred Hutchinson Cancer Research Center, Department of Pathobiology, University of Washington, 1100 Fairview Avenue N, M5C800, PO Box 19024, Seattle, WA 98109, USA
J Cell Sci 119:3435-42. 2006..These data suggest that phosphorylation of Cx43 at serine residues 325, 328 and/or 330 influences channel permselectivity and regulates the efficiency of gap junction assembly... - Cellular interaction of integrin alpha3beta1 with laminin 5 promotes gap junctional communicationP D Lampe
Divisions of Basic Sciences and Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
J Cell Biol 143:1735-47. 1998..We suggest that adhesion of epithelial cells to laminin 5 in the basement membrane via alpha3beta1 promotes GJIC that integrates individual cells into synchronized epiboles... - The effects of connexin phosphorylation on gap junctional communicationPaul D Lampe
Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North DE 320, Box 19024, Seattle, WA 98109, USA
Int J Biochem Cell Biol 36:1171-86. 2004..In several cases, use of site-directed mutants of these sites have shown that these specific phosphorylation events can be linked to changes in gap junctional communication... - Phosphorylation of connexin43 on serine368 by protein kinase C regulates gap junctional communicationP D Lampe
Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
J Cell Biol 149:1503-12. 2000..These data strongly suggest that PKC directly phosphorylates Cx43 on S368 in vivo, which results in a change in single channel behavior that contributes to a decrease in intercellular communication... 
Gap junction assembly: PTX-sensitive G proteins regulate the distribution of connexin43 within cellsP D Lampe
Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Am J Physiol Cell Physiol 281:C1211-22. 2001..These results suggest that PTX-sensitive G proteins regulate connexin43 trafficking, and, as a result of inhibition with PTX, the number of plasma membrane hemichannels available for GJ assembly is reduced...- Altered tumor biology and tumorigenesis in irradiated and chemical carcinogen-treated single and combined connexin32/p27Kip1-deficient miceTimothy J King
Molecular Diagnostics Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
Cell Commun Adhes 12:293-305. 2005..Furthermore, these studies demonstrate that different carcinogens interact disparately with Cx32/p27Kip1 genotypic backgrounds in situ resulting in varied tumorigenic response... - Temporal regulation of connexin phosphorylation in embryonic and adult tissuesTimothy J King
Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M5C800, Box 19024, Seattle, WA 98109, USA
Biochim Biophys Acta 1719:24-35. 2005..These new antibody tools and those under development will allow us to correlate specific phosphorylation events with changes in connexin function... - Quantifying peptide signal in MALDI-TOF mass spectrometry dataTimothy W Randolph
Department of Biostatistics, University of Washington, Seattle, Washington 98195, USA
Mol Cell Proteomics 4:1990-9. 2005.... - Effect of a 12-month exercise intervention on patterns of cellular proliferation in colonic crypts: a randomized controlled trialAnne McTiernan
Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M4 B402, Seattle, WA 98109 1024, USA
Cancer Epidemiol Biomarkers Prev 15:1588-97. 2006..A hypothesized mechanism for exercise-induced colon cancer risk reduction might be through alterations in colon crypt cell architecture and proliferation... - Exercise effect on weight and body fat in men and womenAnne McTiernan
Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, M4 B402, Seattle, WA 98109 1024, USA
Obesity (Silver Spring) 15:1496-512. 2007..The effect of national exercise recommendations on adiposity is unknown and may differ by sex. We examined long-term effects of aerobic exercise on adiposity in women and men... - Key connexin 43 phosphorylation events regulate the gap junction life cycleJoell L Solan
Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
J Membr Biol 217:35-41. 2007..However, phosphorylation at S262 could explain the shift. By defining these phosphorylation events, we have begun to sort out the critical signaling pathways that regulate gap junction function... - Phosphorylation at S365 is a gatekeeper event that changes the structure of Cx43 and prevents down-regulation by PKCJoell L Solan
Molecular Diagnostics Program and 2Proteomics Shared Resource, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
J Cell Biol 179:1301-9. 2007..Thus, phosphorylation at S365 can serve a "gatekeeper" function that may represent a mechanism to protect cells from ischemia and phorbol ester-induced down-regulation of channel conductance... - Risk factors for colorectal cancer in relation to number and size of aberrant crypt foci in humansRebecca E Rudolph
Veterans Affairs Puget Sound Health Care System, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
Cancer Epidemiol Biomarkers Prev 14:605-8. 2005..Future research should be directed toward developing techniques to identify ACF that are likely to progress to cancer and the modifiable factors that promote or inhibit such progression... - Deficiency in the gap junction protein connexin32 alters p27Kip1 tumor suppression and MAPK activation in a tissue-specific mannerTimothy J King
Cancer Prevention Research Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Oncogene 24:1718-26. 2005..This study demonstrates that tissues dependent on Cx32 tumor suppression, such as the liver and lung, exhibit altered tumorigenesis and tumor biology (MAPK pathway activation) related to p27 status... - The gap junction protein connexin32 is a mouse lung tumor suppressorTimothy J King
Cancer Prevention Research Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, and Department of Pathobiology, University of Washington, Seattle, Washington, USA
Cancer Res 64:7191-6. 2004..001) compared with wild-type tumors, implicating this signaling pathway in Cx32/gap junction intercellular communication-associated lung tumorigenesis... - Connexin43 phosphorylation at S368 is acute during S and G2/M and in response to protein kinase C activationJoell L Solan
Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
J Cell Sci 116:2203-11. 2003..Thus, both gap junctional communication and S368 phosphorylation change during S phase and G2/M, implying that phosphorylation at S368 might play a role in key cell-cycle events... - Mice deficient for the gap junction protein Connexin32 exhibit increased radiation-induced tumorigenesis associated with elevated mitogen-activated protein kinase (p44/Erk1, p42/Erk2) activationTimothy J King
Cancer Prevention Research Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Carcinogenesis 25:669-80. 2004.... - New developments in the gap junction field: report from the 2002 ASCB meetingPaul Lampe
Department of Pathobiology, Fred Hutchinson Cancer Research Center, Seattle, WA, USA
Cell Commun Adhes 10:55-8. 2003 - Casein kinase 1 regulates connexin-43 gap junction assemblyCynthia D Cooper
Fred Hutchinson Cancer Research Center and Department of Pathobiology, University of Washington, Seattle, Washington 98109 1024, USA
J Biol Chem 277:44962-8. 2002..These data suggest CK1delta could regulate Cx43 gap junction assembly by directly phosphorylating Cx43... - Connexin 43 in LA-25 cells with active v-src is phosphorylated on Y247, Y265, S262, S279/282, and S368 via multiple signaling pathwaysJoell L Solan
Molecular Diagnostics Program, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Cell Commun Adhes 15:75-84. 2008..These results indicate all three signaling pathways could contribute to gap junction down-regulation during src transformation in LA-25 cells... - Connexin phosphorylation as a regulatory event linked to gap junction channel assemblyJoell L Solan
Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M5C800, Box 19024, Seattle, Washington 98109, United States
Biochim Biophys Acta 1711:154-63. 2005.... - Protein kinase C spatially and temporally regulates gap junctional communication during human wound repair via phosphorylation of connexin43 on serine368Theresa S Richards
Division of Basic and Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
J Cell Biol 167:555-62. 2004..Our evidence indicates that PKC-dependent phosphorylation of Cx43 at S368 creates dynamic communication compartments that can temporally and spatially regulate wound healing... - Connexin43 phosphorylation: structural changes and biological effectsJoell L Solan
Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M5C800 Box 19024, Seattle, WA 98109, USA
Biochem J 419:261-72. 2009..The present review assesses our current understanding of the effects of phosphorylation on connexin43 structure and function that in turn regulate gap junction biology, with an emphasis on events occurring in heart and skin... - Inducible expression of the gap junction protein connexin43 decreases the neoplastic potential of HT-1080 human fibrosarcoma cells in vitro and in vivoTimothy J King
Cancer Prevention Research Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Mol Carcinog 35:29-41. 2002..This study also further supports the hypothesis that loss of Cx43/GJIC in human tumors may play an important role in the dysregulation of normal growth control... - Soy protein containing isoflavones does not decrease colorectal epithelial cell proliferation in a randomized controlled trialKenneth F Adams
Fred Hutchinson Cancer Research Center, Seattle, WA, USA
Am J Clin Nutr 82:620-6. 2005.... - Evaluation of matrix-assisted laser desorption/ionization-time of flight mass spectrometry proteomic profiling: identification of alpha 2-HS glycoprotein B-chain as a biomarker of dietBreeana L Mitchell
Fred Hutchinson Cancer Research Center, University of Washington, Seattle, 98109, USA
Proteomics 5:2238-46. 2005..cruciferous) with 76% accuracy. The 2740 m/z peak was identified as the B-chain of alpha 2-HS glycoprotein, a serum protein previously found to vary with diet and be involved in insulin resistance and immune function... - Connexin 43 interacts with zona occludens-1 and -2 proteins in a cell cycle stage-specific mannerDeepika Singh
Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
J Biol Chem 280:30416-21. 2005..Since essentially all of the Cx43 in G0 cells is assembled into Triton X-100-resistant junctions, Cx43-ZO-1 interaction may contribute to their stability... - Effect of a 12-month exercise intervention on the apoptotic regulating proteins Bax and Bcl-2 in colon crypts: a randomized controlled trialKristin L Campbell
Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, M4 B402, Seattle, WA 98109 1024, USA
Cancer Epidemiol Biomarkers Prev 16:1767-74. 2007..The objective of this study is to examine the effects of a 12-month aerobic exercise program on expression of proteins that promote (Bax) or inhibit (Bcl-2) apoptosis in colon crypts... - Methodologies for characterizing phosphoproteins by mass spectrometryPhilip R Gafken
Proteomics Facility, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Cell Commun Adhes 13:249-62. 2006.... - Identification of connexin-43 interacting proteinsDeepika Singh
Fred Hutchinson Cancer Research Center and Pathobiology Department, University of Washington, Seattle, WA 98109, USA
Cell Commun Adhes 10:215-20. 2003..Further in vitro/in vivo analysis of these interacting proteins will help in our understanding of the global role of connexins in regulating development, cell metabolism and growth... - No effect of exercise on colon mucosal prostaglandin concentrations: a 12-month randomized controlled trialPage E Abrahamson
Cancer Prevention, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
Cancer Epidemiol Biomarkers Prev 16:2351-6. 2007..We hypothesized that an exercise intervention would decrease prostaglandin E(2) concentrations and increase prostaglandin F(2alpha) in colon biopsies compared with controls... - Gap junction remodeling and cardiac arrhythmogenesis in a murine model of oculodentodigital dysplasiaNellie Kalcheva
Leon H Charney Division of Cardiology, New York University School of Medicine, New York, NY 10016, USA
Proc Natl Acad Sci U S A 104:20512-6. 2007..In summary, our data demonstrate that the I130T mutation interferes with Cx43 posttranslational processing, resulting in diminished cell-cell coupling, slowing of impulse propagation, and a proarrhythmic substrate... - The C-terminus of connexin43 adopts different conformations in the Golgi and gap junction as detected with structure-specific antibodiesGina E Sosinsky
National Center for Microscopy and Imaging Research, Center for Research in Biological Systems, University of California, San Diego, La Jolla, CA 92093 0608, USA
Biochem J 408:375-85. 2007..These studies indicate that the conformation of Ser364/Ser365 is important for intracellular localization, whereas the tertiary structure of Pro375-Asp379 is essential in targeting and regulation of gap junctional connexin43... - Selectivity of connexin 43 channels is regulated through protein kinase C-dependent phosphorylationJose F Ek-Vitorin
Department of Physiology, University of Arizona, Tucson 85724, USA
Circ Res 98:1498-505. 2006..Our data suggest that the selectivity of gap junction channels at intercalated disks is increased early in ischemia... - Gap junctions between cells expressing connexin 43 or 32 show inverse permselectivity to adenosine and ATPGary S Goldberg
Department of Physiology and Biophysics, State University of New York, Stony Brook, New York 11794 8661, USA
J Biol Chem 277:36725-30. 2002..This suggests functional consequence because the energy status of a cell could be controlled via connexin expression and channel formation... - Enhanced myocyte contractility and Ca2+ handling in a calcineurin transgenic model of heart failureGuoxiang Chu
Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, 231 Albert B. Sabin Way, OH 45267-0575, USA
Cardiovasc Res 54:105-16. 2002..CONCLUSION: Despite enhanced SR Ca2+ handling and contractility in myocytes, pathological remodeling and defects in intercellular coupling may underlie contractile dysfunction of the calcineurin hearts...
Research Grants
- PHOSPHORYLATION OF GAP JUNCTION PROTEINSPaul D Lampe; Fiscal Year: 2010....
- PHOSPHORYLATION OF GAP JUNCTION PROTEINSPaul Lampe; Fiscal Year: 2007..We hypothesize that Cx43 phosphorylation changes are linked to these processes in a way that affects cellular function. ..
- Proteomic Biomarkers of Health BehaviorsPaul Lampe; Fiscal Year: 2005..abstract_text> ..
- PHOSPHORYLATION OF GAP JUNCTION PROTEINSPaul Lampe; Fiscal Year: 2005..We plan to apply our knowledge of connexin phosphorylation and phosphorylation specific probes to the analysis of connexin status in wounded skin and the remodeling of gap junctions during heart disease. ..
- PHOSPHORYLATION OF GAP JUNCTION PROTEINSPaul Lampe; Fiscal Year: 2000....
- PHOSPHORYLATION OF GAP JUNCTION PROTEINSPaul Lampe; Fiscal Year: 2009..We hypothesize that Cx43 phosphorylation changes are linked to these processes in a way that affects cellular function. ..