- Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fluidPrasanna D Khot
Fred Hutchinson Cancer Research Center, Seattle, WA, USA
BMC Infect Dis 8:73. 2008..It is important to adopt quality control measures in PCR assays to address false positives and negatives which can hinder accurate evaluation of diagnostic performance...
- PCR-based diagnosis of human fungal infectionsPrasanna D Khot
Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109 1024, USA
Expert Rev Anti Infect Ther 7:1201-21. 2009..Finally, we evaluate broad-range fungal PCR assays capable of detecting a wide spectrum of human pathogens...
- Sequencing and analysis of fungal rRNA operons for development of broad-range fungal PCR assaysPrasanna D Khot
Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, Seattle, WA 98109, USA
Appl Environ Microbiol 75:1559-65. 2009..These PCR assays hold promise for detection and identification of fungal pathogens in human tissues...
- A small subpopulation of blastospores in candida albicans biofilms exhibit resistance to amphotericin B associated with differential regulation of ergosterol and beta-1,6-glucan pathway genesPrasanna D Khot
Department of Microbiology and Center for Biofilm Engineering, 366 EPS, Montana State University, Bozeman, MT 59717 3980, USA
Antimicrob Agents Chemother 50:3708-16. 2006..We hypothesize that the resistance originates from a synergistic effect involving changes in both the cell membrane and the cell wall...