Genomes and Genes
Affiliation: Fred Hutchinson Cancer Research Center
- Optimizing viable leukocyte sampling from the female genital tract for clinical trials: an international multi-site studyLyle R McKinnon
Department of Medicine, University of Toronto, Toronto, Canada Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya
PLoS ONE 9:e85675. 2014..However, the best female genital tract sampling technique is unclear...
- Preventing mucosal HIV transmission with topical microbicides: challenges and opportunitiesFlorian Hladik
Department of Obstetrics and Gynecology, University of Washington, 1959 NE Pacific Street, Seattle, WA 98195, USA
Antiviral Res 88:S3-9. 2010..This article forms part of a special supplement on presentations covering HIV transmission and microbicides, based on the symposium "Trends in Microbicide Formulations", held on 25 and 26 January 2010, Arlington, VA...
- HIV infection of the genital mucosa in womenFlorian Hladik
Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, D3 100, Seattle, WA 98109 1024, USA
Curr HIV/AIDS Rep 6:20-8. 2009..Studies using human explant tissues and in vivo animal studies have improved our understanding of the cellular and molecular pathways of infection; this information could be harnessed to design effective HIV vaccines and microbicides...
- Atopic dermatitis with increased severity along a line of BlaschkoFlorian Hladik
Division of Immunology, Department of Dermatology, Allergy and Infectious Diseases, University of Vienna Medical School, Vienna, Austria
J Am Acad Dermatol 53:S221-4. 2005..Individuals such as our patient offer an opportunity to identify intraindividual genetic variations marking loci involved in the pathogenesis of atopy and atopic eczema...
- Repeat-region polymorphisms in the gene for the dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin-related molecule: effects on HIV-1 susceptibilityHuanliang Liu
Department of Laboratory Medicine, University of Washington School of Medicine, Seattle, Washington 98195 8070, USA
J Infect Dis 193:698-702. 2006..5% in high-risk HIV-1-seronegative individuals vs. 27.6% in HIV-1-seropositive individuals; P=.0291). These findings suggest that DC-SIGNR polymorphisms may influence susceptibility to HIV-1...
- Persistence of HIV-1 receptor-positive cells after HSV-2 reactivation is a potential mechanism for increased HIV-1 acquisitionJia Zhu
Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
Nat Med 15:886-92. 2009..The persistence and enrichment of HIV receptor-positive inflammatory cells in the genitalia help explain the inability of anti-HSV-2 therapy to reduce HIV acquisition...
- Ex vivo comparison of microbicide efficacies for preventing HIV-1 genomic integration in intraepithelial vaginal cellsM Juliana McElrath
Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Antimicrob Agents Chemother 54:763-72. 2010..8 microg/ml). These results highlight the merit of our model for screening the mucosal efficacies of novel microbicides and their formulations and potentially rank ordering candidates for clinical evaluation...
- Vaginal langerhans cells nonproductively transporting HIV-1 mediate infection of T cellsLamar Ballweber
Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, D3 100, Seattle, WA 98109 1024, USA
J Virol 85:13443-7. 2011..Transport by migratory LCs to local lymphatics in a nonproductive but infectious form may aid HIV-1 in evasion of topical microbicides that target its intracellular productive life cycle...
- Combined effect of CCR5-Delta32 heterozygosity and the CCR5 promoter polymorphism -2459 A/G on CCR5 expression and resistance to human immunodeficiency virus type 1 transmissionFlorian Hladik
Fred Hutchinson Cancer Research Center, Clinical Research Division, Program in Infectious Diseases, 1100 Fairview Ave N, P O Box 19024, D3 100, Seattle, WA 98109 1024, USA
J Virol 79:11677-84. 2005..We conclude that the CCR5 ORF delta32/wt-CCR5 -2459 A/G genotype combination offers an advantage in resisting sexual HIV-1 transmission and that this effect is mediated by a relative paucity of CCR5 on potential target cells of HIV-1...
- Initial events in establishing vaginal entry and infection by human immunodeficiency virus type-1Florian Hladik
Program in Infectious Diseases, Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Immunity 26:257-70. 2007..Our findings shed light on the very earliest steps of mucosal HIV infection in vivo and may guide the design of effective strategies to block local transmission and prevent HIV-1 spread...
- Impaired viral entry cannot explain reduced CD4+ T cell susceptibility to HIV type 1 in certain highly exposed individualsEmily C Speelmon
Medical Scientist Training Program, University of Washington, Seattle, Washington 98105, USA
AIDS Res Hum Retroviruses 24:1415-27. 2008..Identifying the host-virus interactions responsible for diminished in vitro susceptibility may contribute to the development of novel therapeutic strategies...
- Virus-specific CD8+ T cells accumulate near sensory nerve endings in genital skin during subclinical HSV-2 reactivationJia Zhu
Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
J Exp Med 204:595-603. 2007..These findings indicate that the frequency and clinical course of HSV-2 reactivation in humans is influenced by virus-specific CD8(+) T cells that persist in peripheral mucosa and genital skin after resolution of herpes lesions...
- Most DC-SIGNR transcripts at mucosal HIV transmission sites are alternatively spliced isoformsHuanliang Liu
Department of Laboratory Medicine, University of Washington School of Medicine, Seattle, WA 98195, USA
Eur J Hum Genet 13:707-15. 2005..Soluble isoforms, in particular, may modulate the efficiency of viral transmission and dissemination...
- Comprehensive assessment of HIV target cells in the distal human gut suggests increasing HIV susceptibility toward the anusM J McElrath
Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
J Acquir Immune Defic Syndr 63:263-71. 2013..Yet, little is known about the specific target-cell milieu in the human rectum other than inferences made from the colon...
- Vpu-deficient HIV strains stimulate innate immune signaling responses in target cellsBrian P Doehle
University of Washington School of Medicine, Department of Immunology, Seattle, Washington, USA
J Virol 86:8499-506. 2012..These observations show that HIV-1 can indeed be recognized as a pathogen in infected cells and provide a novel and effective platform for defining the native innate immune programs of target cells of HIV-1 infection...
- Human immunodeficiency virus type 1 mediates global disruption of innate antiviral signaling and immune defenses within infected cellsBrian P Doehle
University of Washington School of Medicine, Department of Immunology, Seattle, WA 98195, USA
J Virol 83:10395-405. 2009..IRF-3 may direct an innate antiviral response that regulates HIV-1 replication and viral set point while governing susceptibility to opportunistic virus coinfections...
- Setting the stage: host invasion by HIVFlorian Hladik
Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Nat Rev Immunol 8:447-57. 2008..Furthermore, we discuss the gaps in our knowledge that are relevant to future studies, which will shape strategies for effective HIV prevention...
- Most highly exposed seronegative men lack HIV-1-specific, IFN-gamma-secreting T cellsFlorian Hladik
Program in Infectious Diseases, Clinical Research Division, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, D3 100, Seattle, WA 98109, USA
J Immunol 171:2671-83. 2003..HIV-1-specific, IFN-gamma-secreting T cells are thus unlikely to substantially contribute to resistance against infection in most exposed seronegative men having sex with men...
- Mechanisms of Sexual HIV-1 TransmissionFlorian Hladik; Fiscal Year: 2006..These studies will improve our knowledge of the human genital immune system and shed light on the mechanisms of HIV-l targeting and strain selection in the female genital tract. ..
- Mechanisms of HIV-1 Transmission in the Genital Mucosa of WomenFlorian Hladik; Fiscal Year: 2008..In this proposal, we will use a novel organ culture model of human vaginal tissue obtained from surgeries to characterize the entry pathways of HIV during the earliest stages of sexual transmission. [unreadable] [unreadable] [unreadable]..