Steven M Hahn

Summary

Affiliation: Fred Hutchinson Cancer Research Center
Country: USA

Publications

  1. pmc Architecture of the RNA polymerase II preinitiation complex and mechanism of ATP-dependent promoter opening
    Sebastian Grünberg
    Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    Nat Struct Mol Biol 19:788-96. 2012
  2. pmc Architecture of the yeast RNA polymerase II open complex and regulation of activity by TFIIF
    James Fishburn
    Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    Mol Cell Biol 32:12-25. 2012
  3. pmc Transcriptional regulation in Saccharomyces cerevisiae: transcription factor regulation and function, mechanisms of initiation, and roles of activators and coactivators
    Steven Hahn
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genetics 189:705-36. 2011
  4. pmc Structure and mechanism of the RNA polymerase II transcription machinery
    Steven Hahn
    Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, 1100 Fairview Ave N, A1 162, Seattle, Washington 98109, USA
    Nat Struct Mol Biol 11:394-403. 2004
  5. pmc Transcriptional regulation. Meeting on regulatory mechanisms in eukaryotic transcription
    Steven Hahn
    Fred Hutchinson Cancer Research Centre, 1100 Fairview Avenue North, Seattle, Washington 98109, USA
    EMBO Rep 9:612-6. 2008
  6. ncbi request reprint Mapping the location of TFIIB within the RNA polymerase II transcription preinitiation complex: a model for the structure of the PIC
    Hung Ta Chen
    Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, 1100 Fairview Avenue North, Seattle, WA 98109 USA
    Cell 119:169-80. 2004
  7. pmc Position of the general transcription factor TFIIF within the RNA polymerase II transcription preinitiation complex
    Jesse Eichner
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    EMBO J 29:706-16. 2010
  8. pmc The transcription elongation factor TFIIS is a component of RNA polymerase II preinitiation complexes
    Bong Kim
    Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA
    Proc Natl Acad Sci U S A 104:16068-73. 2007
  9. ncbi request reprint Binding of TFIIB to RNA polymerase II: Mapping the binding site for the TFIIB zinc ribbon domain within the preinitiation complex
    Hung Ta Chen
    Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, 1100 Fairview Avenue N, Mail stop A1 162, Seattle, WA 98109, USA
    Mol Cell 12:437-47. 2003
  10. pmc A DNA-tethered cleavage probe reveals the path for promoter DNA in the yeast preinitiation complex
    Gail Miller
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, Seattle, Washington 98109, USA
    Nat Struct Mol Biol 13:603-10. 2006

Collaborators

  • Elton T Young
  • ASEEM ANSARI
  • Bruce A Knutson
  • Hung Ta Chen
  • James Fishburn
  • Linda Warfield
  • Ying Liu
  • Jeffrey A Ranish
  • P Geetha Rani
  • Sebastian Grünberg
  • Jesse Eichner
  • Bong Kim
  • Kevan M Shokat
  • Gail Miller
  • Ruedi Aebersold
  • Jie Luo
  • Jasmina Allen
  • Jeffrey Ranish
  • Walter H Lang
  • Chao Zhang
  • Alexey I Nesvizhskii
  • Neeman Mohibullah
  • Charles Kung
  • Jimmy Eng
  • Xiao Jun Li
  • Eugene C Yi
  • Yu Lu

Detail Information

Publications17

  1. pmc Architecture of the RNA polymerase II preinitiation complex and mechanism of ATP-dependent promoter opening
    Sebastian Grünberg
    Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    Nat Struct Mol Biol 19:788-96. 2012
    ..Right-handed threading of DNA through the Ssl2 binding groove, combined with the fixed position of upstream promoter DNA, leads to DNA unwinding and the open state...
  2. pmc Architecture of the yeast RNA polymerase II open complex and regulation of activity by TFIIF
    James Fishburn
    Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    Mol Cell Biol 32:12-25. 2012
    ..Together, our results show an unexpected architecture of minimal open complexes and the regulation of activity by TFIIF and the TFIIB core domain...
  3. pmc Transcriptional regulation in Saccharomyces cerevisiae: transcription factor regulation and function, mechanisms of initiation, and roles of activators and coactivators
    Steven Hahn
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genetics 189:705-36. 2011
    ..We also examine advances in understanding the RNA polymerase II transcription machinery, conserved coactivator complexes, transcription activation domains, and the cooperation of these factors in gene regulatory mechanisms...
  4. pmc Structure and mechanism of the RNA polymerase II transcription machinery
    Steven Hahn
    Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, 1100 Fairview Ave N, A1 162, Seattle, Washington 98109, USA
    Nat Struct Mol Biol 11:394-403. 2004
    ....
  5. pmc Transcriptional regulation. Meeting on regulatory mechanisms in eukaryotic transcription
    Steven Hahn
    Fred Hutchinson Cancer Research Centre, 1100 Fairview Avenue North, Seattle, Washington 98109, USA
    EMBO Rep 9:612-6. 2008
  6. ncbi request reprint Mapping the location of TFIIB within the RNA polymerase II transcription preinitiation complex: a model for the structure of the PIC
    Hung Ta Chen
    Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, 1100 Fairview Avenue North, Seattle, WA 98109 USA
    Cell 119:169-80. 2004
    ..The TFIIF subunit Tfg1 was found in close proximity to the TFIIB B finger, linker, and core domains, suggesting that these two factors closely cooperate during initiation...
  7. pmc Position of the general transcription factor TFIIF within the RNA polymerase II transcription preinitiation complex
    Jesse Eichner
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    EMBO J 29:706-16. 2010
    ..Consistent with this mechanism, mutations far from the enzyme active site, which alter the binding of either structured TFIIF domains to Pol II, have similar defects in transcription start site usage...
  8. pmc The transcription elongation factor TFIIS is a component of RNA polymerase II preinitiation complexes
    Bong Kim
    Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA
    Proc Natl Acad Sci U S A 104:16068-73. 2007
    ..The results demonstrate that TFIIS is a PIC component that is required for efficient formation and/or stability of the complex...
  9. ncbi request reprint Binding of TFIIB to RNA polymerase II: Mapping the binding site for the TFIIB zinc ribbon domain within the preinitiation complex
    Hung Ta Chen
    Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, 1100 Fairview Avenue N, Mail stop A1 162, Seattle, WA 98109, USA
    Mol Cell 12:437-47. 2003
    ..This surface is best conserved in polymerases that require a TFIIB-like factor. Our results suggest a general mechanism for interaction of TFIIB-like factors and RNA polymerases and a mechanism for the function of the ribbon domain...
  10. pmc A DNA-tethered cleavage probe reveals the path for promoter DNA in the yeast preinitiation complex
    Gail Miller
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, Seattle, Washington 98109, USA
    Nat Struct Mol Biol 13:603-10. 2006
    ..Together, our results show how the general factors and Pol II converge on promoter DNA within the PIC...
  11. ncbi request reprint Function of a eukaryotic transcription activator during the transcription cycle
    James Fishburn
    Howard Hughes Medical Institute, 1100 Fairview Ave North, Mailstop A1 162, Seattle, Washington 98109, USA
    Mol Cell 18:369-78. 2005
    ..Thus, a single activating region contacts multiple factors, and each contact makes differential contributions to transcriptional activation...
  12. pmc Two cyclin-dependent kinases promote RNA polymerase II transcription and formation of the scaffold complex
    Ying Liu
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle, Washington 98109, USA
    Mol Cell Biol 24:1721-35. 2004
    ..In addition to the previously known substrate, the Pol II CTD, it was found that Kin28 phosphorylates two subunits of Mediator and Srb10 targets two subunits of TFIID for phosphorylation...
  13. pmc RNA polymerase II (Pol II)-TFIIF and Pol II-mediator complexes: the major stable Pol II complexes and their activity in transcription initiation and reinitiation
    P Geetha Rani
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Mol Cell Biol 24:1709-20. 2004
    ..These results suggest that both the Pol II-Med and Pol II-TFIIF complexes can be recruited for transcription initiation but that only the Pol II-TFIIF complex is competent for transcription reinitiation...
  14. pmc Domains of Tra1 important for activator recruitment and transcription coactivator functions of SAGA and NuA4 complexes
    Bruce A Knutson
    Fred Hutchinson Cancer Research Center, Division of Basic Sciences, Seattle, WA 98109, USA
    Mol Cell Biol 31:818-31. 2011
    ..Our results show that Tra1 recruitment at Gcn4-dependent and Rap1-dependent promoters requires the same regions of Tra1 and that separate regions of Tra1 contribute to the HAT activity and stability of the SAGA and NuA4 HAT modules...
  15. pmc Phosphorylation of the transcription elongation factor Spt5 by yeast Bur1 kinase stimulates recruitment of the PAF complex
    Ying Liu
    Fred Hutchinson Cancer Research Center, Division of Basic Sciences, Seattle, Washington 98109, USA
    Mol Cell Biol 29:4852-63. 2009
    ..Genetic results suggest that Bur1 is essential for growth because it targets multiple factors that play distinct roles in transcription...
  16. ncbi request reprint Identification of TFB5, a new component of general transcription and DNA repair factor IIH
    Jeffrey A Ranish
    Institute for Systems Biology, 1441 North 34th Street, Seattle, Washington 98103 8904, USA
    Nat Genet 36:707-13. 2004
    ..The identification of a new, evolutionarily conserved, core TFIIH subunit is essential for our understanding of TFIIH function in transcription, DNA repair and human disease...
  17. pmc The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex
    Hung Ta Chen
    Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, Mailstop A1 162, Seattle, Washington 98109, USA
    Nat Struct Mol Biol 14:696-703. 2007
    ..Together with previous biochemical and structural studies, these findings illuminate the structural organization of the PIC and the network of protein-protein interactions involved in transcription start site selection...

Research Grants20

  1. Transcriptional Regulation During Cell Growth Differentiation and Development
    Steven Hahn; Fiscal Year: 2006
    ..The conference will also provide an up to date view of one of the most rapidly moving fields in biology. ..
  2. Molecular Analysis of Eukaryotic Transcription
    Steven Hahn; Fiscal Year: 2005
    ..Analog-sensitive versions of these kinases will be utilized to identify relevant kinase targets. In vitro and in vivo assays will be used to determine the mechanisms whereby kinase activity regulates transcription. ..
  3. Mechanisms of eukaryotic transcription activation
    Steven Hahn; Fiscal Year: 2007
    ..Our proposed work will illuminate important mechanisms and principles of transcriptional regulation. ..
  4. Mechanisms of eukaryotic transcription activation
    Steven Hahn; Fiscal Year: 2005
    ..Our proposed work will illuminate important mechanisms and principles of transcriptional regulation. ..
  5. Molecular Analysis of Eukaryotic Transcription
    Steven M Hahn; Fiscal Year: 2010
    ....
  6. Molecular analysis of RNAP II and III transcription
    Steven Hahn; Fiscal Year: 2002
    ..Finally, the target of the zinc ribbon domains in TFIIB and Brf will be identified, comparing the role of these domains in Pol II and III transcription. ..
  7. Molecular Analysis of Eukaryotic Transcription
    Steven Hahn; Fiscal Year: 2007
    ..Analog-sensitive versions of these kinases will be utilized to identify relevant kinase targets. In vitro and in vivo assays will be used to determine the mechanisms whereby kinase activity regulates transcription. ..
  8. Molecular Analysis of Eukaryotic Transcription
    Steven Hahn; Fiscal Year: 2009
    ....
  9. Mechanisms of eukaryotic transcription activation
    Steven Hahn; Fiscal Year: 2006
    ..Our proposed work will illuminate important mechanisms and principles of transcriptional regulation. ..
  10. Molecular Analysis of Eukaryotic Transcription
    Steven Hahn; Fiscal Year: 2006
    ..Analog-sensitive versions of these kinases will be utilized to identify relevant kinase targets. In vitro and in vivo assays will be used to determine the mechanisms whereby kinase activity regulates transcription. ..
  11. Molecular analysis of RNAP II and III transcription
    Steven Hahn; Fiscal Year: 2004
    ..Finally, the target of the zinc ribbon domains in TFIIB and Brf will be identified, comparing the role of these domains in Pol II and III transcription. ..
  12. Molecular analysis of RNAP II and III transcription
    Steven Hahn; Fiscal Year: 2003
    ..Finally, the target of the zinc ribbon domains in TFIIB and Brf will be identified, comparing the role of these domains in Pol II and III transcription. ..
  13. Molecular analysis of RNAP II and III transcription
    Steven Hahn; Fiscal Year: 2001
    ..Finally, the target of the zinc ribbon domains in TFIIB and Brf will be identified, comparing the role of these domains in Pol II and III transcription. ..
  14. MOLECULAR ANALYSIS OF RNAPIII TRANSCRIPTION INITIATION
    Steven Hahn; Fiscal Year: 1999
    ..His work will involve molecular techniques (gene cloning, mutagenesis, and PCR), biochemical fractionation, in vitro transcription, protein biochemistry, and yeast molecular genetics. ..
  15. Mechanisms of eukaryotic transcription activation
    Steven M Hahn; Fiscal Year: 2010
    ....