DAVID NEAL FREDRICKS

Summary

Affiliation: Fred Hutchinson Cancer Research Center
Country: USA

Publications

  1. pmc Detection of hydrogen peroxide-producing Lactobacillus species in the vagina: a comparison of culture and quantitative PCR among HIV-1 seropositive women
    Jennifer E Balkus
    Department of Epidemiology, University of Washington, Box 359909, 325 9th Avenue, Seattle, WA 98104, USA
    BMC Infect Dis 12:188. 2012
  2. pmc Comparison of quantitative real time PCR with Sequencing and ribosomal RNA-FISH for the identification of fungi in formalin fixed, paraffin-embedded tissue specimens
    Volker Rickerts
    Department of Internal Medicine II, University Hospital, Frankfurt, Germany
    BMC Infect Dis 11:202. 2011
  3. pmc Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid
    Prasanna D Khot
    Fred Hutchinson Cancer Research Center, Seattle, WA, USA
    BMC Infect Dis 8:73. 2008
  4. pmc Comparison of oral and vaginal metronidazole for treatment of bacterial vaginosis in pregnancy: impact on fastidious bacteria
    Caroline M Mitchell
    Department of Obstetrics and Gynecology, University of Washington, Seattle, WA, USA
    BMC Infect Dis 9:89. 2009
  5. pmc Targeted PCR for detection of vaginal bacteria associated with bacterial vaginosis
    David N Fredricks
    Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue, North, D3 100, Box 19024, Seattle, WA 98109 1024, USA
    J Clin Microbiol 45:3270-6. 2007
  6. ncbi request reprint Molecular identification of bacteria associated with bacterial vaginosis
    David N Fredricks
    Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    N Engl J Med 353:1899-911. 2005
  7. pmc Comparison of six DNA extraction methods for recovery of fungal DNA as assessed by quantitative PCR
    David N Fredricks
    Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    J Clin Microbiol 43:5122-8. 2005
  8. ncbi request reprint Molecular identification of an invasive gingival bacterial community
    David N Fredricks
    Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    Clin Infect Dis 41:e1-4. 2005
  9. pmc Molecular methods to describe the spectrum and dynamics of the vaginal microbiota
    David N Fredricks
    Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division, Seattle, WA 98109 1024, USA
    Anaerobe 17:191-5. 2011
  10. pmc Changes in vaginal bacterial concentrations with intravaginal metronidazole therapy for bacterial vaginosis as assessed by quantitative PCR
    David N Fredricks
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    J Clin Microbiol 47:721-6. 2009

Research Grants

Collaborators

Detail Information

Publications15

  1. pmc Detection of hydrogen peroxide-producing Lactobacillus species in the vagina: a comparison of culture and quantitative PCR among HIV-1 seropositive women
    Jennifer E Balkus
    Department of Epidemiology, University of Washington, Box 359909, 325 9th Avenue, Seattle, WA 98104, USA
    BMC Infect Dis 12:188. 2012
    ..We assessed the concordance of H(2)O(2)-producing Lactobacillus detected by culture with quantitative PCR (qPCR) detection of Lactobacillus species commonly assumed to be H(2)O(2)-producers...
  2. pmc Comparison of quantitative real time PCR with Sequencing and ribosomal RNA-FISH for the identification of fungi in formalin fixed, paraffin-embedded tissue specimens
    Volker Rickerts
    Department of Internal Medicine II, University Hospital, Frankfurt, Germany
    BMC Infect Dis 11:202. 2011
    ....
  3. pmc Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid
    Prasanna D Khot
    Fred Hutchinson Cancer Research Center, Seattle, WA, USA
    BMC Infect Dis 8:73. 2008
    ..It is important to adopt quality control measures in PCR assays to address false positives and negatives which can hinder accurate evaluation of diagnostic performance...
  4. pmc Comparison of oral and vaginal metronidazole for treatment of bacterial vaginosis in pregnancy: impact on fastidious bacteria
    Caroline M Mitchell
    Department of Obstetrics and Gynecology, University of Washington, Seattle, WA, USA
    BMC Infect Dis 9:89. 2009
    ..Both oral and intravaginal metronidazole can be used to treat bacterial vaginosis in pregnancy, but little is known about the impact of different routes of antibiotic administration on concentrations of fastidious vaginal bacteria...
  5. pmc Targeted PCR for detection of vaginal bacteria associated with bacterial vaginosis
    David N Fredricks
    Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue, North, D3 100, Box 19024, Seattle, WA 98109 1024, USA
    J Clin Microbiol 45:3270-6. 2007
    ..PCR detection of one or more fastidious bacterial species is a more reliable indicator of BV than detection of bacteria, such as Gardnerella vaginalis, previously linked to BV, highlighting the potential of PCR for the diagnosis of BV...
  6. ncbi request reprint Molecular identification of bacteria associated with bacterial vaginosis
    David N Fredricks
    Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    N Engl J Med 353:1899-911. 2005
    ..The cause of bacterial vaginosis remains poorly understood despite numerous studies based on cultures. Bacteria in microbial communities can be identified without cultivation by characterizing their ribosomal DNA (rDNA) sequences...
  7. pmc Comparison of six DNA extraction methods for recovery of fungal DNA as assessed by quantitative PCR
    David N Fredricks
    Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    J Clin Microbiol 43:5122-8. 2005
    ..In conclusion, the six extraction methods produce markedly differing yields of fungal DNA and thus can significantly affect the results of fungal PCR assays. No single extraction method was optimal for all organisms...
  8. ncbi request reprint Molecular identification of an invasive gingival bacterial community
    David N Fredricks
    Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
    Clin Infect Dis 41:e1-4. 2005
    ..Oral bacterial flora may cause invasive gingival disease with hyperplasia in immunocompromised patients...
  9. pmc Molecular methods to describe the spectrum and dynamics of the vaginal microbiota
    David N Fredricks
    Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division, Seattle, WA 98109 1024, USA
    Anaerobe 17:191-5. 2011
    ..Longitudinal studies have demonstrated that the vaginal microbiota can be highly dynamic, with dramatic shifts in bacterial composition and concentrations in response to numerous endogenous and exogenous factors...
  10. pmc Changes in vaginal bacterial concentrations with intravaginal metronidazole therapy for bacterial vaginosis as assessed by quantitative PCR
    David N Fredricks
    Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    J Clin Microbiol 47:721-6. 2009
    ....
  11. pmc Relationship of specific vaginal bacteria and bacterial vaginosis treatment failure in women who have sex with women
    Jeanne M Marrazzo
    University of Washington and the Fred Hutchinson Cancer Research Center, Seattle, Washington, USA
    Ann Intern Med 149:20-8. 2008
    ..Bacterial vaginosis frequently persists after treatment. The role of newly defined bacterial vaginosis-associated bacteria (BVAB), which have a specificity for this condition of 97% or greater, has not been assessed...
  12. pmc Diversity of human vaginal bacterial communities and associations with clinically defined bacterial vaginosis
    Brian B Oakley
    Program in Infectious Diseases, D3 100, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, Seattle, WA 98109 1024, USA
    Appl Environ Microbiol 74:4898-909. 2008
    ..The human vagina hosts many bacteria that are only distantly related to known species, and subjects with BV harbor particularly taxon-rich and diverse bacterial communities...
  13. ncbi request reprint Breakthrough fungal infections in stem cell transplant recipients receiving voriconazole
    Alexander Imhof
    Program in Infectious Diseases, Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Clin Infect Dis 39:743-6. 2004
    ..Minimal inhibitory concentrations were > or =1 microg/mL for all available isolates. Yeasts and molds with decreased susceptibility to voriconazole may cause invasive infection in patients treated successfully for aspergillosis...
  14. ncbi request reprint Cultivation of Tropheryma whipplei from cerebrospinal fluid
    Matthias Maiwald
    Department of Microbiology, Stanford University School of Medicine, Stanford, California, USA
    J Infect Dis 188:801-8. 2003
    ..This first isolation of T. whipplei from CSF provides clear evidence of viable bacteria in the central nervous system in individuals with WD, even after prolonged antibiotic therapy...
  15. ncbi request reprint Human herpesvirus 8 and sarcoidosis
    David N Fredricks
    Clin Infect Dis 34:559-60. 2002

Research Grants13

  1. The Molecular Microbiology of Bacterial Vaginosis
    David Fredricks; Fiscal Year: 2003
    ..In future studies, vaginal fluid samples from a larger cohort of women will be assayed to determine if a candidate pathogen is specifically associated with BV. ..
  2. Novel Cultivation Methods for the Domestication of Vaginal Bacteria
    DAVID NEAL FREDRICKS; Fiscal Year: 2010
    ....
  3. The Role of Uncultivated Bacteria in Bacterial Vaginosis
    DAVID NEAL FREDRICKS; Fiscal Year: 2010
    ..abstract_text> ..
  4. Quantitative PCR for the Detection of Fungal Infections
    David Fredricks; Fiscal Year: 2007
    ..Hypothesis: Fungal 18S rDNA qPCR employing a broad range fungal probe, or a system of multiple specific probes, will be useful for detecting emerging fungal pathogens in the transplant setting. ..
  5. The Role of Uncultivated Bacteria in Bacterial Vaginosis
    David Fredricks; Fiscal Year: 2007
    ....
  6. Novel Computational Tools for Studying the Human Microbiome
    FREDERICK ALBERT MATSEN; Fiscal Year: 2010
    ....