Stephen C De Rosa

Summary

Affiliation: Fred Hutchinson Cancer Research Center
Country: USA

Publications

  1. pmc Vaccine applications of flow cytometry
    Stephen C De Rosa
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
    Methods 57:383-91. 2012
  2. pmc HIV-DNA priming alters T cell responses to HIV-adenovirus vaccine even when responses to DNA are undetectable
    Stephen C De Rosa
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
    J Immunol 187:3391-401. 2011
  3. pmc Optimization and validation of an 8-color intracellular cytokine staining (ICS) assay to quantify antigen-specific T cells induced by vaccination
    Helen Horton
    Program in Infectious Diseases, Fred Hutchinson Cancer Research Center FHCRC, Seattle, WA 98109, USA
    J Immunol Methods 323:39-54. 2007
  4. pmc Optimization and qualification of a multiplex bead array to assess cytokine and chemokine production by vaccine-specific cells
    Olivier D Defawe
    Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    J Immunol Methods 382:117-28. 2012
  5. pmc Development of an automated analysis system for data from flow cytometric intracellular cytokine staining assays from clinical vaccine trials
    Nicholas Shulman
    LabKey Software Foundation, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Cytometry A 73:847-56. 2008
  6. doi request reprint Association between peripheral γδ T-cell profile and disease progression in individuals infected with HIV-1 or HIV-2 in West Africa
    Natalie N Zheng
    Department of Pathology, University of Washington, Seattle, WA, USA
    J Acquir Immune Defic Syndr 57:92-100. 2011
  7. pmc HIV-1 vaccine-induced immunity in the test-of-concept Step Study: a case-cohort analysis
    M Juliana McElrath
    Vaccine and Infectious Disease Institute and the HIV Vaccine Trials Network, Fred Hutchinson Cancer Research Center, Seattle, WA, USA
    Lancet 372:1894-905. 2008
  8. pmc Merck Ad5/HIV induces broad innate immune activation that predicts CD8⁺ T-cell responses but is attenuated by preexisting Ad5 immunity
    Daniel E Zak
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Proc Natl Acad Sci U S A 109:E3503-12. 2012
  9. ncbi request reprint Preservation of T cell proliferation restricted by protective HLA alleles is critical for immune control of HIV-1 infection
    Helen Horton
    Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    J Immunol 177:7406-15. 2006
  10. pmc Diversity in CD8(+) T cell function and epitope breadth among persons with genital herpes
    Kerry J Laing
    Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA
    J Clin Immunol 30:703-22. 2010

Collaborators

Detail Information

Publications17

  1. pmc Vaccine applications of flow cytometry
    Stephen C De Rosa
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
    Methods 57:383-91. 2012
    ..Cell sorting of vaccine-induced T cells and B cells is used to allow genomic and transcriptional analysis of these cells. Thus, flow cytometric methods are commonly used in trials testing the next generation of vaccines...
  2. pmc HIV-DNA priming alters T cell responses to HIV-adenovirus vaccine even when responses to DNA are undetectable
    Stephen C De Rosa
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
    J Immunol 187:3391-401. 2011
    ....
  3. pmc Optimization and validation of an 8-color intracellular cytokine staining (ICS) assay to quantify antigen-specific T cells induced by vaccination
    Helen Horton
    Program in Infectious Diseases, Fred Hutchinson Cancer Research Center FHCRC, Seattle, WA 98109, USA
    J Immunol Methods 323:39-54. 2007
    ..In conclusion, we provide the first description of a quantitatively validated ICS assay, which permits quantitative and qualitative evaluation of vaccine-induced immunogenicity and analysis of immune correlates of protection...
  4. pmc Optimization and qualification of a multiplex bead array to assess cytokine and chemokine production by vaccine-specific cells
    Olivier D Defawe
    Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    J Immunol Methods 382:117-28. 2012
    ..This assay provides an excellent complement to the existing repertoire of assays for assessing immunogenicity in HIV vaccine clinical trials...
  5. pmc Development of an automated analysis system for data from flow cytometric intracellular cytokine staining assays from clinical vaccine trials
    Nicholas Shulman
    LabKey Software Foundation, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Cytometry A 73:847-56. 2008
    ..Routine use of the system does not require specialized expertise. This high-throughput analysis will provide great utility for rapid evaluation of complex multiparameter flow cytometric measurements collected from large clinical trials...
  6. doi request reprint Association between peripheral γδ T-cell profile and disease progression in individuals infected with HIV-1 or HIV-2 in West Africa
    Natalie N Zheng
    Department of Pathology, University of Washington, Seattle, WA, USA
    J Acquir Immune Defic Syndr 57:92-100. 2011
    ..Human gammadelta (γδ) T cells play an important role in protective immunity in HIV-1 and simian immunodeficiency virus infection; their role in HIV-2 infection is unknown...
  7. pmc HIV-1 vaccine-induced immunity in the test-of-concept Step Study: a case-cohort analysis
    M Juliana McElrath
    Vaccine and Infectious Disease Institute and the HIV Vaccine Trials Network, Fred Hutchinson Cancer Research Center, Seattle, WA, USA
    Lancet 372:1894-905. 2008
    ..We assessed vaccine-induced immunity and its potential contributions to infection risk...
  8. pmc Merck Ad5/HIV induces broad innate immune activation that predicts CD8⁺ T-cell responses but is attenuated by preexisting Ad5 immunity
    Daniel E Zak
    Seattle Biomedical Research Institute, Seattle, WA 98109, USA
    Proc Natl Acad Sci U S A 109:E3503-12. 2012
    ..When applied iteratively, the findings may permit selection of HIV vaccine candidates eliciting innate immune response profiles more likely to drive HIV protective immunity...
  9. ncbi request reprint Preservation of T cell proliferation restricted by protective HLA alleles is critical for immune control of HIV-1 infection
    Helen Horton
    Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    J Immunol 177:7406-15. 2006
    ..Our findings suggest that the capacity to maintain CD8+ T cell proliferation, regardless of MHC-restriction, may serve as an important correlate of disease protection in the event of infection following vaccination...
  10. pmc Diversity in CD8(+) T cell function and epitope breadth among persons with genital herpes
    Kerry J Laing
    Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA
    J Clin Immunol 30:703-22. 2010
    ..The diversity in T cell response in these ex vivo assessments offers the potential of defining immune correlates of HSV-2 reactivation in humans...
  11. ncbi request reprint Gamma delta+ T cells involvement in viral immune control of chronic human herpesvirus 8 infection
    Serge Barcy
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98109, USA
    J Immunol 180:3417-25. 2008
    ..These data indicate that gammadelta T cells play a role in both innate and adaptive T cell responses against HHV-8 in immunocompetent individuals...
  12. pmc ORFeome approach to the clonal, HLA allele-specific CD4 T-cell response to a complex pathogen in humans
    Lichen Jing
    Department of Medicine, University of Washington, 1616 Eastlake Avenue East, Suite 251, Seattle, WA 98102, USA
    J Immunol Methods 347:36-45. 2009
    ....
  13. pmc Human adenovirus-specific T cells modulate HIV-specific T cell responses to an Ad5-vectored HIV-1 vaccine
    Nicole Frahm
    Vaccine and Infectious Disease Division and HIV Vaccine Trials Network, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 1024, USA
    J Clin Invest 122:359-67. 2012
    ..These findings provide what we believe to be a new understanding of how preexisting viral immunity may impact the efficacy of vaccines under current evaluation for prevention of HIV, tuberculosis, and malaria...
  14. pmc Silibinin inhibits HIV-1 infection by reducing cellular activation and proliferation
    Janela McClure
    Department of Laboratory Medicine, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 7:e41832. 2012
    ..Silymarin-derived compounds provide cytoprotection by suppressing virus infection, immune activation, and inflammation, and as such may be relevant for both HIV mono-infected and HIV/HCV co-infected subjects...
  15. ncbi request reprint Multicolor immunophenotyping: human mature immune system
    Stephen C De Rosa
    University of Washington School of Medicine, Department of Laboratory Medicine, Seattle, Washington 98109, USA
    Methods Cell Biol 75:577-94. 2004
  16. ncbi request reprint Does preserving memory correlate with surviving HIV?
    Helen Horton
    Program in Infectious Diseases, Fred Hutchinson Cancer Research Center FHCRC, Seattle, WA 98109, USA
    Trends Microbiol 15:1-3. 2007
    ....
  17. pmc HIV-1-specific enzyme-linked immunosorbent spot assay responses in HIV-1-exposed uninfected partners in discordant relationships compared to those in low-risk controls
    Brandon L Guthrie
    Department of Epidemiology, University of Washington, Seattle, Washington, USA
    Clin Vaccine Immunol 19:1798-805. 2012
    ..238), were not associated with responses in HESN. HIV-1-specific cellular responses may be less relevant to resistance to infection among HESN who are using risk reduction strategies that decrease their direct viral exposure...