E K Jaffe

Summary

Affiliation: Fox Chase Cancer Center
Country: USA

Publications

  1. ncbi request reprint An artificial gene for human porphobilinogen synthase allows comparison of an allelic variation implicated in susceptibility to lead poisoning
    E K Jaffe
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    J Biol Chem 275:2619-26. 2000
  2. ncbi request reprint Characterization of the role of the stimulatory magnesium of Escherichia coli porphobilinogen synthase
    E K Jaffe
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111
    Biochemistry 34:244-51. 1995
  3. ncbi request reprint Mechanistic implications of mutations to the active site lysine of porphobilinogen synthase
    L W Mitchell
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    J Biol Chem 276:1538-44. 2001
  4. ncbi request reprint Mechanistic basis for suicide inactivation of porphobilinogen synthase by 4,7-dioxosebacic acid, an inhibitor that shows dramatic species selectivity
    J Kervinen
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA
    Biochemistry 40:8227-36. 2001
  5. ncbi request reprint The molecular mechanism of lead inhibition of human porphobilinogen synthase
    E K Jaffe
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    J Biol Chem 276:1531-7. 2001
  6. ncbi request reprint 13C NMR studies of the enzyme-product complex of Bacillus subtilis chorismate mutase
    J S Rajagopalan
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111
    Biochemistry 32:3965-72. 1993
  7. ncbi request reprint Porphobilinogen synthase from Escherichia coli is a Zn(II) metalloenzyme stimulated by Mg(II)
    L W Mitchell
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111
    Arch Biochem Biophys 300:169-77. 1993

Collaborators

Detail Information

Publications7

  1. ncbi request reprint An artificial gene for human porphobilinogen synthase allows comparison of an allelic variation implicated in susceptibility to lead poisoning
    E K Jaffe
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    J Biol Chem 275:2619-26. 2000
    ..However, there was no differential displacement of Zn(II) by Pb(II) between K59 and N59, and simple Pb(II) inhibition studies revealed no allelic difference...
  2. ncbi request reprint Characterization of the role of the stimulatory magnesium of Escherichia coli porphobilinogen synthase
    E K Jaffe
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111
    Biochemistry 34:244-51. 1995
    ..The addition of unlabeled or 1-13C-labeled ALA does not significantly affect the Mn(II) EPR spectra.(ABSTRACT TRUNCATED AT 250 WORDS)..
  3. ncbi request reprint Mechanistic implications of mutations to the active site lysine of porphobilinogen synthase
    L W Mitchell
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    J Biol Chem 276:1538-44. 2001
    ..Thus, formation of the lysine to ALA Schiff base is not required to initiate the asymmetry that results in half-site reactivity...
  4. ncbi request reprint Mechanistic basis for suicide inactivation of porphobilinogen synthase by 4,7-dioxosebacic acid, an inhibitor that shows dramatic species selectivity
    J Kervinen
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, Pennsylvania 19111, USA
    Biochemistry 40:8227-36. 2001
    ..coli numbering). This is the first structure to show inhibitor binding at the second of two substrate-binding sites...
  5. ncbi request reprint The molecular mechanism of lead inhibition of human porphobilinogen synthase
    E K Jaffe
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    J Biol Chem 276:1531-7. 2001
    ..The data support a novel model where a high affinity lead site is a hybrid of the ZnA and ZnB sites. We propose that the lone electron pair of Pb(II) precludes Pb(II) to function in PBGS catalysis...
  6. ncbi request reprint 13C NMR studies of the enzyme-product complex of Bacillus subtilis chorismate mutase
    J S Rajagopalan
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111
    Biochemistry 32:3965-72. 1993
    ..abstract truncated at 250 words)..
  7. ncbi request reprint Porphobilinogen synthase from Escherichia coli is a Zn(II) metalloenzyme stimulated by Mg(II)
    L W Mitchell
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111
    Arch Biochem Biophys 300:169-77. 1993
    ..Li, and M. P. Timko (1991) J. Biol. Chem. 266, 17060-17066]. This work lays the foundation for identification of functional amino acids based on the sequence similarities between PBGS from bacterial, plant, and mammalian sources...