Richard Hardy

Summary

Affiliation: Fox Chase Cancer Center
Country: USA

Publications

  1. ncbi request reprint The protean nature of cells in the B lymphocyte lineage
    Richard R Hardy
    The Division of Basic Sciences, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    Immunity 26:703-14. 2007
  2. pmc Lineage specification and plasticity in CD19- early B cell precursors
    Lynn L Rumfelt
    Division of Basic Sciences, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    J Exp Med 203:675-87. 2006
  3. doi request reprint Positive and negative selection of natural autoreactive B cells
    Richard R Hardy
    Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA
    Adv Exp Med Biol 750:227-38. 2012
  4. ncbi request reprint B-1 B cell development
    Richard R Hardy
    Division of Basic Sciences, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    J Immunol 177:2749-54. 2006
  5. ncbi request reprint B-1 B cells: development, selection, natural autoantibody and leukemia
    Richard R Hardy
    Division of Basic Science, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    Curr Opin Immunol 18:547-55. 2006
  6. ncbi request reprint Development of B cells producing natural autoantibodies to thymocytes and senescent erythrocytes
    Richard R Hardy
    Division of Basic Sciences, Fox Chase Cancer Center, 333 Cottman Ave, Philadelphia, PA 19111, USA
    Springer Semin Immunopathol 26:363-75. 2005
  7. ncbi request reprint Selection during development of VH11+ B cells: a model for natural autoantibody-producing CD5+ B cells
    Richard R Hardy
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA 19111 2497, USA
    Immunol Rev 197:60-74. 2004
  8. ncbi request reprint B-cell commitment: deciding on the players
    Richard R Hardy
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA
    Curr Opin Immunol 15:158-65. 2003
  9. ncbi request reprint Development of VH11+ B cells: a model for selection of B cells producing natural autoantibodies
    Richard R Hardy
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA, USA
    Curr Dir Autoimmun 6:196-211. 2003
  10. ncbi request reprint B cell development pathways
    R R Hardy
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Ave, Philadelphia, Pennsylvania 19111, USA
    Annu Rev Immunol 19:595-621. 2001

Research Grants

Collaborators

Detail Information

Publications34

  1. ncbi request reprint The protean nature of cells in the B lymphocyte lineage
    Richard R Hardy
    The Division of Basic Sciences, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    Immunity 26:703-14. 2007
    ....
  2. pmc Lineage specification and plasticity in CD19- early B cell precursors
    Lynn L Rumfelt
    Division of Basic Sciences, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    J Exp Med 203:675-87. 2006
    ..A) stage, and B/T lymphoid plasticity persists until the CD19+ pro-B stage. Thus, MLP, CLP, and Fr. A represent progressively B lineage-specified stages in development, before the CD19+ B lineage-committed pro-B stage...
  3. doi request reprint Positive and negative selection of natural autoreactive B cells
    Richard R Hardy
    Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA
    Adv Exp Med Biol 750:227-38. 2012
    ....
  4. ncbi request reprint B-1 B cell development
    Richard R Hardy
    Division of Basic Sciences, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    J Immunol 177:2749-54. 2006
    ....
  5. ncbi request reprint B-1 B cells: development, selection, natural autoantibody and leukemia
    Richard R Hardy
    Division of Basic Science, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    Curr Opin Immunol 18:547-55. 2006
    ..The relationship of normal B-1 cells with B-cell lymphomas and leukemias continues to be a subject of interest and debate...
  6. ncbi request reprint Development of B cells producing natural autoantibodies to thymocytes and senescent erythrocytes
    Richard R Hardy
    Division of Basic Sciences, Fox Chase Cancer Center, 333 Cottman Ave, Philadelphia, PA 19111, USA
    Springer Semin Immunopathol 26:363-75. 2005
    ....
  7. ncbi request reprint Selection during development of VH11+ B cells: a model for natural autoantibody-producing CD5+ B cells
    Richard R Hardy
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA 19111 2497, USA
    Immunol Rev 197:60-74. 2004
    ..Together, these constrain VH11 generation to fetal development and may favor production of B cells with the prototype VH11Vkappa9 BCR...
  8. ncbi request reprint B-cell commitment: deciding on the players
    Richard R Hardy
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA
    Curr Opin Immunol 15:158-65. 2003
    ..Increasingly powerful flow cytometry analysis of genetically altered animals (knockouts and transgenics) combined with sensitive functional assays may provide an answer, a "scorecard" for the cell stages...
  9. ncbi request reprint Development of VH11+ B cells: a model for selection of B cells producing natural autoantibodies
    Richard R Hardy
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA, USA
    Curr Dir Autoimmun 6:196-211. 2003
  10. ncbi request reprint B cell development pathways
    R R Hardy
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Ave, Philadelphia, Pennsylvania 19111, USA
    Annu Rev Immunol 19:595-621. 2001
    ....
  11. ncbi request reprint Response by B cell precursors to pre-B receptor assembly: differences between fetal liver and bone marrow
    R R Hardy
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    Curr Top Microbiol Immunol 252:25-30. 2000
    ..The consequence is very distinct primary repertoires for development of fetal B1 cells and adult bone marrow B2 cells...
  12. ncbi request reprint B-cell commitment, development and selection
    R R Hardy
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    Immunol Rev 175:23-32. 2000
    ..Finally, we describe a new VH3609 antithymocyte Ig transgenic mouse model system that has provided the first definitive evidence for the role of self-antigen in development and maintenance of natural autoreactive B cells...
  13. ncbi request reprint A single VH gene is utilized predominantly in anti-BrMRBC hybridomas derived from purified Ly-1 B cells. Definition of the VH11 family
    R R Hardy
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA 19111
    J Immunol 142:3643-51. 1989
    ....
  14. ncbi request reprint Absence of MHC class II expression distinguishes fetal from adult B lymphopoiesis in mice
    K Hayakawa
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA 19111
    J Immunol 152:4801-7. 1994
    ..Furthermore, we suggest that such distinct programs of class II expression during fetal and adult lymphopoiesis could result in differences in susceptibility to tolerance...
  15. pmc Commitment to the B lymphoid lineage occurs before DH-JH recombination
    D Allman
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    J Exp Med 189:735-40. 1999
    ....
  16. ncbi request reprint Positive selection of natural autoreactive B cells
    K Hayakawa
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA
    Science 285:113-6. 1999
    ..Thus, B cells can be subject to positive selection, generated, and maintained on the basis of their autoreactivity...
  17. ncbi request reprint Resolution of three nonproliferative immature splenic B cell subsets reveals multiple selection points during peripheral B cell maturation
    D Allman
    Department of Pathology and Laboratory Medicine and Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Immunol 167:6834-40. 2001
    ....
  18. ncbi request reprint TCR beta chain influences but does not solely control autoreactivity of V alpha 14J281T cells
    M Gui
    Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    J Immunol 167:6239-46. 2001
    ..Thus, heterogeneity of Valpha14+ T cell reactivity is due to both beta-chain diversity and control mechanism(s) beyond primary TCR structure...
  19. pmc HD mice: a novel mouse mutant with a specific defect in the generation of CD4(+) T cells
    V P Dave
    Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA
    Proc Natl Acad Sci U S A 95:8187-92. 1998
    ..This is the first genetic defect of its kind to be described in the mouse and may prove highly informative in understanding the molecular pathways underlying lineage commitment...
  20. ncbi request reprint Early B cell factor cooperates with Runx1 and mediates epigenetic changes associated with mb-1 transcription
    Holly Maier
    Integrated Department of Immunology, National Jewish Medical and Research Center, Denver, Colorado 80206, USA
    Nat Immunol 5:1069-77. 2004
    ..EBF function was enhanced by interaction with the transcription factor Runx1. These data suggest a molecular basis for the hierarchical dependence of Pax5 function on EBF and E2A in B lymphocyte development...
  21. doi request reprint Isolation of Ly-1+/CD5+ B cells by cell sorting
    Richard R Hardy
    Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA
    Curr Protoc Immunol . 2003
    ..g., size and internal granularity) and correlated expression of several surface molecules...
  22. ncbi request reprint B cell receptor basal signaling regulates antigen-induced Ig light chain rearrangements
    Brian R Schram
    Center for Immunology, Department of Medicine, University of Minnesota Medical School, University of Minnesota, Minneapolis, MN 55455, USA
    J Immunol 180:4728-41. 2008
    ..These findings support a model whereby Ag-induced receptor editing is inhibited by BCR basal signaling on developing B cells; BCR down-regulation removes this basal signal, thereby initiating receptor editing...
  23. pmc Autoreactive B-1 B cells: constraints on natural autoantibody B cell antigen receptors
    Ben Rowley
    Division of Basic Science, Fox Chase Cancer Center, 333 Cottman Ave, Philadelphia, PA 19111, USA
    J Autoimmun 29:236-45. 2007
    ..Here, we examine another possible bottleneck to the B1 cell repertoire: light chain pairing with V(H)11 heavy chain, finding very significant preferences...
  24. pmc Human chronic lymphocytic leukemia modeled in mouse by targeted TCL1 expression
    Roberta Bichi
    Kimmel Cancer Center, Jefferson Medical College, 233 South 10th Street, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 99:6955-60. 2002
    ..Our findings provide an animal model for CLL, the most common human leukemia, and demonstrate that deregulation of the Tcl1 pathway plays a crucial role in CLL pathogenesis...
  25. ncbi request reprint Ablation of ribosomal protein L22 selectively impairs alphabeta T cell development by activation of a p53-dependent checkpoint
    Stephen J Anderson
    Division of Immunology and Hematology, Lexicon Genetics, Inc, 8800 Technology Forest Place, The Woodlands, TX 77381, USA
    Immunity 26:759-72. 2007
    ....
  26. pmc Control of the B cell-intrinsic tolerance programs by ubiquitin ligases Cbl and Cbl-b
    Yasuyuki Kitaura
    Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Immunity 26:567-78. 2007
    ..Thus, Cbl proteins control B cell-intrinsic checkpoint of immune tolerance, possibly through coordinating multiple BCR-proximal signaling pathways during anergy induction...
  27. pmc Positive selection of anti-thy-1 autoreactive B-1 cells and natural serum autoantibody production independent from bone marrow B cell development
    Kyoko Hayakawa
    Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    J Exp Med 197:87-99. 2003
    ..These findings demonstrate that B-1 positive selection, resulting in the production of natural serum ATA, arises independently from the major pathway of BM B cell development and selection...
  28. ncbi request reprint Characterization of B lymphopoiesis in mouse bone marrow and spleen
    Richard R Hardy
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA, USA
    Methods Mol Biol 271:1-24. 2004
    ....
  29. ncbi request reprint Surface phenotype of Peyer's patch germinal center cells: implications for the role of germinal centers in B cell differentiation. 1982
    Eugene C Butcher
    J Immunol 175:1363-72. 2005
  30. ncbi request reprint Association of B-1 B cells with follicular dendritic cells in spleen
    Lijun Wen
    Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    J Immunol 174:6918-26. 2005
    ..These results indicate that B-1 B cells are the first B cells to express fully mature levels of CXCR5, thereby promoting the development of FDC...
  31. pmc Basal immunoglobulin signaling actively maintains developmental stage in immature B cells
    Lina E Tze
    Center for Immunology, University of Minnesota Medical School, Minneapolis, Minnesota, USA
    PLoS Biol 3:e82. 2005
    ..These studies identify a previously unappreciated level of plasticity in the B cell developmental program, and have important implications for our understanding of central tolerance mechanisms...
  32. ncbi request reprint Evidence of marginal-zone B cell-positive selection in spleen
    Lijun Wen
    Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111, USA
    Immunity 23:297-308. 2005
    ..These data indicate that positive selection can occur in developing B cells and that BCR signal strength is a key factor in deciding between two functionally distinct mature B cell compartments in the microenvironment of the spleen...
  33. pmc Pax5 and linker histone H1 coordinate DNA methylation and histone modifications in the 3' regulatory region of the immunoglobulin heavy chain locus
    Vincenzo Giambra
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Mol Cell Biol 28:6123-33. 2008
    ..Palindromic enhancers become demethylated later in B-cell differentiation, in B and plasma cells...
  34. doi request reprint The Immunological Genome Project: networks of gene expression in immune cells
    Tracy S P Heng
    Section on Immunology and Immunogenetics, Joslin Diabetes Center and Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02215, USA
    Nat Immunol 9:1091-4. 2008
    ..The Immunological Genome Project combines immunology and computational biology laboratories in an effort to establish a complete 'road map' of gene-expression and regulatory networks in all immune cells...

Research Grants31

  1. LY-1 B CELLS: DEVELOPMENTAL ORIGINS AND V GENE BIASES
    Richard R Hardy; Fiscal Year: 2010
    ....
  2. BD Biosciences LSR Flow Cytometer
    Richard Hardy; Fiscal Year: 2002
    ..abstract_text> ..
  3. LY-1 B CELLS: DEVELOPMENTAL ORIGINS AND V GENE BIASES
    Richard Hardy; Fiscal Year: 2005
    ..abstract_text> ..
  4. IMMUNOGLOBULIN V GENES AND B CELL DEVELOPMENT
    Richard Hardy; Fiscal Year: 2006
    ..The results of these studies will help to establish the types of selection that shape the expressed B cell repertoire, both fetally and in the adult, with implications for modulating normal and autoimmune responses. ..
  5. LY-1 B CELLS: DEVELOPMENTAL ORIGINS AND V GENE BIASES
    Richard Hardy; Fiscal Year: 2007
    ....
  6. IMMUNOGLOBULIN V GENES AND B CELL DEVELOPMENT
    Richard Hardy; Fiscal Year: 2001
    ..The results of these studies will help to establish the types of selection that shape the expressed B cell repertoire, fetally and in the adult, with implications for modulating both normal and autoimmune responses. ..
  7. LY-1 B-CELLS--DEVELOPMENTAL ORIGINS AND V GENE BIASES
    Richard Hardy; Fiscal Year: 1993
    ..This work may eventually help us to understand the relationship of special features of fetal B cell development with autoreactivity nd B cell neoplasia...
  8. LY-1 B CELLS--LINEAGE DETERMINATION/ANTIBODY REPERTOIRE
    Richard Hardy; Fiscal Year: 1992
    ..Specificities encoded by these V genes to be examined in detail will include both anti-thymocyte and anti-bromelain-treated mouse erythrocyte autoantibodies, both distinctively enriched in this population...
  9. LY-1 B CELLS--DEVELOPMENTAL ORIGINS AND V GENE
    Richard Hardy; Fiscal Year: 2000
    ..Cumulatively, these studies will provide a clearer picture of fetal and adult B lymphopoiesis and distinguish whether the modes of selection affecting developing B cells are similar or different during these two ontogenic timi s. ..