Ken J Yoshitomi

Summary

Affiliation: Food and Drug Administration
Country: USA

Publications

  1. ncbi request reprint Detection of Shiga toxin genes stx1, stx2, and the +93 uidA mutation of E. coli O157:H7/H-using SYBR Green I in a real-time multiplex PCR
    Ken J Yoshitomi
    Seafood Products Research Center, US Food and Drug Administration, 22201 23rd Drive SE, Bothell, WA 98021 4421, USA
    Mol Cell Probes 20:31-41. 2006
  2. ncbi request reprint Optimization of a 3'-minor groove binder-DNA probe targeting the uidA gene for rapid identification of Escherichia coli O157:H7 using real-time PCR
    Ken J Yoshitomi
    Seafood Products Research Center, US Food and Drug Administration, 22201 23rd Drive SE, Bothell, WA 98021 4421, USA
    Mol Cell Probes 17:275-80. 2003
  3. pmc Multiplex real-time PCR method to identify Shiga toxin genes stx1 and stx2 and Escherichia coli O157:H7/H- serotype
    Karen C Jinneman
    Seafood Products Research Center, Pacific Regional Laboratory Northwest, U S Food and Drug Administration, Bothell, Washington 98021, USA
    Appl Environ Microbiol 69:6327-33. 2003
  4. doi request reprint Detection and isolation of low levels of E. coli O157:H7 in cilantro by real-time PCR, immunomagnetic separation, and cultural methods with and without an acid treatment
    Ken J Yoshitomi
    Food and Drug Administration, Pacific Regional Laboratory, Northwest, Bothell, WA 98021 4421, USA
    J Food Sci 77:M481-9. 2012
  5. doi request reprint Evaluation of shiga toxin-producing Escherichia coli (STEC) method for the detection and identification of STEC O104 strains from sprouts
    Karen C Jinneman
    Applied Technology Center, Pacific Regional Laboratory Northwest, U S Food and Drug Administration, 22201 23rd Dr SE, Bothell, WA 98021, USA
    Food Microbiol 30:321-8. 2012

Detail Information

Publications5

  1. ncbi request reprint Detection of Shiga toxin genes stx1, stx2, and the +93 uidA mutation of E. coli O157:H7/H-using SYBR Green I in a real-time multiplex PCR
    Ken J Yoshitomi
    Seafood Products Research Center, US Food and Drug Administration, 22201 23rd Drive SE, Bothell, WA 98021 4421, USA
    Mol Cell Probes 20:31-41. 2006
    ..The assay was expanded to a panel of 138 isolates consisting of STEC, E. coli O157:H7, non-toxigenic E. coli, and non-E. coli isolates with melt peaks consistent with those stated above...
  2. ncbi request reprint Optimization of a 3'-minor groove binder-DNA probe targeting the uidA gene for rapid identification of Escherichia coli O157:H7 using real-time PCR
    Ken J Yoshitomi
    Seafood Products Research Center, US Food and Drug Administration, 22201 23rd Drive SE, Bothell, WA 98021 4421, USA
    Mol Cell Probes 17:275-80. 2003
    ..coli O157:H7 and non-O157:H7 strains. The improved specificity of MGB probes for single base pair mismatches such as the +93 uidA mutation provides a novel approach towards rapid identification of E. coli O157:H7...
  3. pmc Multiplex real-time PCR method to identify Shiga toxin genes stx1 and stx2 and Escherichia coli O157:H7/H- serotype
    Karen C Jinneman
    Seafood Products Research Center, Pacific Regional Laboratory Northwest, U S Food and Drug Administration, Bothell, Washington 98021, USA
    Appl Environ Microbiol 69:6327-33. 2003
    ..There is more than 98.6% sensitivity and 100% specificity for all three gene targets based on a panel of 138 isolates. The PCR efficiencies were >/= 1.89, and as few as 6 CFU/reaction could be detected...
  4. doi request reprint Detection and isolation of low levels of E. coli O157:H7 in cilantro by real-time PCR, immunomagnetic separation, and cultural methods with and without an acid treatment
    Ken J Yoshitomi
    Food and Drug Administration, Pacific Regional Laboratory, Northwest, Bothell, WA 98021 4421, USA
    J Food Sci 77:M481-9. 2012
    ..Additional techniques such as acidification of enrichment broths can exploit acid resistance characteristics of pathogens such as E. coli O157:H7, facilitating their isolation in complex food matrices...
  5. doi request reprint Evaluation of shiga toxin-producing Escherichia coli (STEC) method for the detection and identification of STEC O104 strains from sprouts
    Karen C Jinneman
    Applied Technology Center, Pacific Regional Laboratory Northwest, U S Food and Drug Administration, 22201 23rd Dr SE, Bothell, WA 98021, USA
    Food Microbiol 30:321-8. 2012
    ..The use of additional serotype-specific real-time PCR assays and supplemental chromogenic media to assist the detection and recovery of these organisms were also evaluated...