Dmitriy V Volokhov

Summary

Affiliation: Food and Drug Administration
Country: USA

Publications

  1. ncbi Mycoplasma testing of cell substrates and biologics: Review of alternative non-microbiological techniques
    Dmitriy V Volokhov
    U S Food and Drug Administration, Center for Biologics Evaluation and Research, Office of Vaccine Research and Review, Division of Viral Products, Laboratory of Methods Development, HFM 470, 1401 Rockville Pike, Rockville, MD 20852, USA
    Mol Cell Probes 25:69-77. 2011
  2. ncbi Lactobacillus brantae sp. nov., isolated from faeces of Canada geese (Branta canadensis)
    Dmitriy V Volokhov
    Center for Biologics Evaluation and Research, US Food and Drug Administration, 1401 Rockville Pike, HFM 470, Rockville, MD 20852, USA
    Int J Syst Evol Microbiol 62:2068-76. 2012
  3. ncbi RNA polymerase beta subunit (rpoB) gene and the 16S-23S rRNA intergenic transcribed spacer region (ITS) as complementary molecular markers in addition to the 16S rRNA gene for phylogenetic analysis and identification of the species of the family Mycoplasm
    Dmitriy V Volokhov
    Center for Biologics Evaluation and Research, US Food and Drug Administration, 1401 Rockville Pike, HFM 470, Rockville, MD 20852, USA
    Mol Phylogenet Evol 62:515-28. 2012
  4. ncbi Genetic analysis of housekeeping genes of members of the genus Acholeplasma: phylogeny and complementary molecular markers to the 16S rRNA gene
    Dmitriy V Volokhov
    Center for Biologics Evaluation and Research, Food and Drug Administration, 1401 Rockville Pike, HFM 470, Rockville, MD 20852, USA
    Mol Phylogenet Evol 44:699-710. 2007
  5. ncbi Biological enrichment of Mycoplasma agents by cocultivation with permissive cell cultures
    Dmitriy V Volokhov
    Center for Biologics Evaluation and Research, Food and Drug Administration, 1401 Rockville Pike, HFM 470, Rockville, Maryland 20852, USA
    Appl Environ Microbiol 74:5383-91. 2008
  6. ncbi Novel hemotrophic mycoplasma identified in naturally infected California sea lions (Zalophus californianus)
    Dmitriy V Volokhov
    Center for Biologics Evaluation and Research, Food and Drug Administration, 1401 Rockville Pike, HFM 470, Rockville, MD 20852, USA
    Vet Microbiol 149:262-8. 2011
  7. ncbi Application of cell culture enrichment for improving the sensitivity of mycoplasma detection methods based on nucleic acid amplification technology (NAT)
    Hyesuk Kong
    Center for Biologics Evaluation and Research, Food and Drug Administration, 1401 Rockville Pike, HFM 470, Rockville, MD 20852, USA
    Appl Microbiol Biotechnol 77:223-32. 2007
  8. ncbi Sequencing of the intergenic 16S-23S rRNA spacer (ITS) region of Mollicutes species and their identification using microarray-based assay and DNA sequencing
    Dmitriy V Volokhov
    Center of Biologics Evaluation and Research, Office of Vaccines Research and Review, Division of Viral Products, Laboratory of Methods Development, US Food and Drug Administration, Rockville, MD 20852, USA
    Appl Microbiol Biotechnol 71:680-98. 2006
  9. ncbi Genotyping of measles virus in clinical specimens on the basis of oligonucleotide microarray hybridization patterns
    Alexander A Neverov
    Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, MD 20852 1448, USA
    J Clin Microbiol 44:3752-9. 2006
  10. ncbi Oligonucleotide microarrays for identification of microbial pathogens and detection of their virulence-associated or drug-resistance determinants
    Dmitriy V Volokhov
    Center for Biologics Evaluation and Research, Food and Drug Administration, Kensington, MD, USA
    Methods Mol Biol 671:55-94. 2011

Collaborators

Detail Information

Publications13

  1. ncbi Mycoplasma testing of cell substrates and biologics: Review of alternative non-microbiological techniques
    Dmitriy V Volokhov
    U S Food and Drug Administration, Center for Biologics Evaluation and Research, Office of Vaccine Research and Review, Division of Viral Products, Laboratory of Methods Development, HFM 470, 1401 Rockville Pike, Rockville, MD 20852, USA
    Mol Cell Probes 25:69-77. 2011
    ..g. from inactivated raw materials, etc.). Our review provides an overview of these alternative methods and discusses the pros and cons of their application for the testing of mycoplasmas in biologics and cell substrates...
  2. ncbi Lactobacillus brantae sp. nov., isolated from faeces of Canada geese (Branta canadensis)
    Dmitriy V Volokhov
    Center for Biologics Evaluation and Research, US Food and Drug Administration, 1401 Rockville Pike, HFM 470, Rockville, MD 20852, USA
    Int J Syst Evol Microbiol 62:2068-76. 2012
    ..nov. is proposed. The type strain is SL1108(T) (= ATCC BAA-2142(T) = LMG 26001(T) = DSM 23927(T)) and two additional strains are SL1170 and SL60106...
  3. ncbi RNA polymerase beta subunit (rpoB) gene and the 16S-23S rRNA intergenic transcribed spacer region (ITS) as complementary molecular markers in addition to the 16S rRNA gene for phylogenetic analysis and identification of the species of the family Mycoplasm
    Dmitriy V Volokhov
    Center for Biologics Evaluation and Research, US Food and Drug Administration, 1401 Rockville Pike, HFM 470, Rockville, MD 20852, USA
    Mol Phylogenet Evol 62:515-28. 2012
    ..We believe that this approach might also become a valuable tool for routine analysis and primary identification of new isolates in medical and veterinary microbiological laboratories...
  4. ncbi Genetic analysis of housekeeping genes of members of the genus Acholeplasma: phylogeny and complementary molecular markers to the 16S rRNA gene
    Dmitriy V Volokhov
    Center for Biologics Evaluation and Research, Food and Drug Administration, 1401 Rockville Pike, HFM 470, Rockville, MD 20852, USA
    Mol Phylogenet Evol 44:699-710. 2007
    ..These data, as well as previously published data on metabolic features of A. multilocale, leads to the proposal to reclassify A. multilocale as a member of the family Entomoplasmataceae...
  5. ncbi Biological enrichment of Mycoplasma agents by cocultivation with permissive cell cultures
    Dmitriy V Volokhov
    Center for Biologics Evaluation and Research, Food and Drug Administration, 1401 Rockville Pike, HFM 470, Rockville, Maryland 20852, USA
    Appl Environ Microbiol 74:5383-91. 2008
    ..We believe that this type of mycoplasma enrichment is one of the most promising approaches for improving the purity and safety testing of cell substrates and other cell-derived biologics and pharmaceuticals...
  6. ncbi Novel hemotrophic mycoplasma identified in naturally infected California sea lions (Zalophus californianus)
    Dmitriy V Volokhov
    Center for Biologics Evaluation and Research, Food and Drug Administration, 1401 Rockville Pike, HFM 470, Rockville, MD 20852, USA
    Vet Microbiol 149:262-8. 2011
    ..nov...
  7. ncbi Application of cell culture enrichment for improving the sensitivity of mycoplasma detection methods based on nucleic acid amplification technology (NAT)
    Hyesuk Kong
    Center for Biologics Evaluation and Research, Food and Drug Administration, 1401 Rockville Pike, HFM 470, Rockville, MD 20852, USA
    Appl Microbiol Biotechnol 77:223-32. 2007
    ....
  8. ncbi Sequencing of the intergenic 16S-23S rRNA spacer (ITS) region of Mollicutes species and their identification using microarray-based assay and DNA sequencing
    Dmitriy V Volokhov
    Center of Biologics Evaluation and Research, Office of Vaccines Research and Review, Division of Viral Products, Laboratory of Methods Development, US Food and Drug Administration, Rockville, MD 20852, USA
    Appl Microbiol Biotechnol 71:680-98. 2006
    ..The microarray analysis of the samples used demonstrated a highly specific assay, which is capable of rapid and accurate discrimination among Mollicutes species...
  9. ncbi Genotyping of measles virus in clinical specimens on the basis of oligonucleotide microarray hybridization patterns
    Alexander A Neverov
    Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, MD 20852 1448, USA
    J Clin Microbiol 44:3752-9. 2006
    ..In addition, the microarray demonstrated the ability to identify potential new genotypes of MV based on the similarity of their hybridization patterns with those of known MV genotypes...
  10. ncbi Oligonucleotide microarrays for identification of microbial pathogens and detection of their virulence-associated or drug-resistance determinants
    Dmitriy V Volokhov
    Center for Biologics Evaluation and Research, Food and Drug Administration, Kensington, MD, USA
    Methods Mol Biol 671:55-94. 2011
    ..In this chapter, we describe key aspects and methodologies important for the development and use of DNA microarrays for microbial diagnostics...
  11. ncbi Molecular detection of drug-resistant Mycobacterium tuberculosis with a scanning-frame oligonucleotide microarray
    Dmitriy V Volokhov
    Center for Biologics Evaluation and Research, US Food and Drug Administration, Rockville, Maryland, USA
    Methods Mol Biol 465:395-417. 2009
    ..Sliding-frame microarrays can be used to identify other drug-resistant TB strains that have mutations in relevant drug resistance genes...
  12. ncbi Evaluation of Mycoplasma inactivation during production of biologics: egg-based viral vaccines as a model
    Selwyn A Wilson David
    Division of Viral Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, HFM 470, Rockville, Maryland 20852, USA
    Appl Environ Microbiol 76:2718-28. 2010
    ....
  13. ncbi The presence of the internalin gene in natural atypically hemolytic Listeria innocua strains suggests descent from L. monocytogenes
    Dmitriy V Volokhov
    FDA, 5100 Paint Branch Pkwy, College Park, MD 20740, USA
    Appl Environ Microbiol 73:1928-39. 2007
    ..innocua strains altered by horizontal transfer. It is more likely that they are distinct relics of the evolution of L. innocua from an ancestral L. monocytogenes, as postulated by others...