Research Topics
| Jeffrey S SmithSummaryAffiliation: Food and Drug Administration Country: USA Publications
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Detail Information
Publications
A quantitative assay for measuring clearance of adenovirus vectors by Kupffer cellsJeffrey S Smith
Division of Cellular and Gene Therapies, Food and Drug Administration, Center for Biologics Evaluation and Research, Bethesda, MD 20892, USA
J Virol Methods 147:54-60. 2008..As an example of the utility of this method, it was found that pre-injecting mice with polyinosinic acid reduces accumulation of Ad in Kupffer cells by approximately 90%...
Rapid Kupffer cell death after intravenous injection of adenovirus vectorsElanchezhiyan Manickan
Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
Mol Ther 13:108-17. 2006..Together these results show that an intravenous injection of adenovirus vector causes synchronous and surprisingly rapid Kupffer cell death...
Interaction of systemically delivered adenovirus vectors with Kupffer cells in mouse liverJeffrey S Smith
Division of Cellular and Gene Therapies, Food and Drug Administration Center for Biologics Evaluation and Research, Bethesda, MD 20892, USA
Hum Gene Ther 19:547-54. 2008..We conclude that Ad vectors kill KCs at a postbinding step and that this cell death can be mitigated if downstream events in viral entry are blocked...
The role of endosomal escape and mitogen-activated protein kinases in adenoviral activation of the innate immune responseJeffrey S Smith
Division of Cellular and Gene Therapies, Food and Drug Administration Center for Biologics Evaluation and Research, Bethesda, Maryland, United States of America
PLoS ONE 6:e26755. 2011..Thus, even though p38 and ERK are rapidly activated after i.v. injection of AdV, cytokine and chemokine responses are mostly independent of these kinases...
Clearance of adenovirus by Kupffer cells is mediated by scavenger receptors, natural antibodies, and complementZhili Xu
Division of Cellular and Gene Therapies, FDA CBER, 8800 Rockville Pike, Bethesda, MD 20892, USA
J Virol 82:11705-13. 2008....
Induction of shock after intravenous injection of adenovirus vectors: a critical role for platelet-activating factorZhili Xu
Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA
Mol Ther 18:609-16. 2010..The identification of this novel pathway suggests strategies to improve the safety of systemic gene therapy with Ad vectors...
Adenovirus activates complement by distinctly different mechanisms in vitro and in vivo: indirect complement activation by virions in vivoJie Tian
Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
J Virol 83:5648-58. 2009..In vitro studies are poorly predictive of in vivo mechanisms because Ad virions activate complement through indirect mechanisms in vivo...
Severe pulmonary pathology after intravenous administration of vectors in cirrhotic ratsJeffrey S Smith
Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
Mol Ther 9:932-41. 2004....
