Research Topics
| J E SlaterSummaryAffiliation: Food and Drug Administration Country: USA Publications
| Collaborators
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Detail Information
Publications
Murine B-cell and T-cell epitopes of the allergen Hev b 5 from natural rubber latexJ E Slater
Center for Molecular Mechanisms of Disease Research, Children s Research Institute, Children s National Medical Center, Washington, DC 20010, USA
Mol Immunol 36:135-43. 1999..Since they may contain major T-cell epitopes but do not exhibit significant antibody binding, peptide regions 38-48 and 75-101 are candidates for specific immunotherapy to Hev b 5 in the BALB/c mouse model...
Characterization of allergen extractsJ E Slater
Laboratory of Immunobiochemistry, Division of Bacterial Parasitic and Allergenic Products, Office of Vaccines Regulation and Research, CBER USFDA, Bethesda, MD, USA
Dev Biol (Basel) 122:145-52. 2005..The surrogate tests differ for different extracts. National reference standards to which manufactured lots are compared are maintained at FDA/CBER. Allergen standardization has facilitated the establishment of data-driven release limits...
Competition enzyme-linked immunosorbant assay (ELISA) can be a sensitive method for the specific detection of small quantities of allergen in a complex mixtureE Dobrovolskaia
Center for Biologics Evaluation and Research, US Food and Drug Administration, Bethesda, MD 20852, USA
Clin Exp Allergy 36:525-30. 2006..CONCLUSION: The competition ELISA can be highly sensitive to compositional differences in complex allergen mixtures, even when the specific detecting antibody is present in relatively small amounts...
Biological potency of German cockroach allergen extracts determined in an inner city populationJ E Slater
Center for Biologics Evaluation and Research, U S Food and Drug Administration, Bethesda, MD, USA
Clin Exp Allergy 37:1033-9. 2007..To perform valid studies on the diagnosis and treatment of cockroach allergy, biological potencies of test extracts need to be established, and a surrogate in vitro test for biological potency should be chosen...
Exploiting the avian immunoglobulin system to simplify the generation of recombinant antibodies to allergenic proteinsW J J Finlay
Center for Biologics Evaluation and Research, US Food and Drug Administration, Bethesda, MD 20892, USA
Clin Exp Allergy 35:1040-8. 2005..The resulting antibody fragments are of demonstrable use in allergen identification and quantification, in comparison with standard immunoassays...
Characterization and comparison of commercially available German and American cockroach allergen extractsM L Patterson
Laboratory of Immunobiochemistry, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Bethesda 20852, USA
Clin Exp Allergy 32:721-7. 2002..In this study we examine the variability among unstandardized cockroach allergen extracts...
Specific monoclonal antibodies and human immunoglobulin E show that Hev b 5 is an abundant allergen in high protein powdered latex glovesM F Sutherland
Department of Allergy, Asthma and Clinical Immunology, The Alfred Hospital and Monash University, Victoria, Australia
Clin Exp Allergy 32:583-9. 2002..This may explain the observed higher frequency of sensitization to this allergen in HCWs...
