J E Slater
Affiliation: Food and Drug Administration
- Murine B-cell and T-cell epitopes of the allergen Hev b 5 from natural rubber latexJ E Slater
Center for Molecular Mechanisms of Disease Research, Children s Research Institute, Children s National Medical Center, Washington, DC 20010, USA
Mol Immunol 36:135-43. 1999..Since they may contain major T-cell epitopes but do not exhibit significant antibody binding, peptide regions 38-48 and 75-101 are candidates for specific immunotherapy to Hev b 5 in the BALB/c mouse model...
- Characterization of allergen extractsJ E Slater
Laboratory of Immunobiochemistry, Division of Bacterial Parasitic and Allergenic Products, Office of Vaccines Regulation and Research, CBER USFDA, Bethesda, MD, USA
Dev Biol (Basel) 122:145-52. 2005..The surrogate tests differ for different extracts. National reference standards to which manufactured lots are compared are maintained at FDA/CBER. Allergen standardization has facilitated the establishment of data-driven release limits...
- Competition enzyme-linked immunosorbant assay (ELISA) can be a sensitive method for the specific detection of small quantities of allergen in a complex mixtureE Dobrovolskaia
Center for Biologics Evaluation and Research, US Food and Drug Administration, Bethesda, MD 20852, USA
Clin Exp Allergy 36:525-30. 2006..CONCLUSION: The competition ELISA can be highly sensitive to compositional differences in complex allergen mixtures, even when the specific detecting antibody is present in relatively small amounts...
- Biological potency of German cockroach allergen extracts determined in an inner city populationJ E Slater
Center for Biologics Evaluation and Research, U S Food and Drug Administration, Bethesda, MD, USA
Clin Exp Allergy 37:1033-9. 2007..To perform valid studies on the diagnosis and treatment of cockroach allergy, biological potencies of test extracts need to be established, and a surrogate in vitro test for biological potency should be chosen...
- Exploiting the avian immunoglobulin system to simplify the generation of recombinant antibodies to allergenic proteinsW J J Finlay
Center for Biologics Evaluation and Research, US Food and Drug Administration, Bethesda, MD 20892, USA
Clin Exp Allergy 35:1040-8. 2005..The resulting antibody fragments are of demonstrable use in allergen identification and quantification, in comparison with standard immunoassays...
- Characterization and comparison of commercially available German and American cockroach allergen extractsM L Patterson
Laboratory of Immunobiochemistry, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Bethesda 20852, USA
Clin Exp Allergy 32:721-7. 2002..In this study we examine the variability among unstandardized cockroach allergen extracts...
- Specific monoclonal antibodies and human immunoglobulin E show that Hev b 5 is an abundant allergen in high protein powdered latex glovesM F Sutherland
Department of Allergy, Asthma and Clinical Immunology, The Alfred Hospital and Monash University, Victoria, Australia
Clin Exp Allergy 32:583-9. 2002..This may explain the observed higher frequency of sensitization to this allergen in HCWs...