Zuben E Sauna

Summary

Affiliation: Food and Drug Administration
Country: USA

Publications

  1. pmc Polymorphisms in the F8 gene and MHC-II variants as risk factors for the development of inhibitory anti-factor VIII antibodies during the treatment of hemophilia a: a computational assessment
    Gouri Shankar Pandey
    Laboratory of Hemostasis, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA
    PLoS Comput Biol 9:e1003066. 2013
  2. pmc Aptamers as a sensitive tool to detect subtle modifications in therapeutic proteins
    Ran Zichel
    Laboratory of Hemostasis, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, United States of America
    PLoS ONE 7:e31948. 2012
  3. pmc Characterization of conformation-sensitive antibodies to ADAMTS13, the von Willebrand cleavage protease
    Zuben E Sauna
    Laboratory of Hemostasis, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, United States of America
    PLoS ONE 4:e6506. 2009
  4. doi request reprint Understanding the contribution of synonymous mutations to human disease
    Zuben E Sauna
    Laboratory of Hemostasis, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, 29 Lincoln Drive, Bethesda, Maryland 20892, USA
    Nat Rev Genet 12:683-91. 2011
  5. ncbi request reprint The conserved tyrosine residues 401 and 1044 in ATP sites of human P-glycoprotein are critical for ATP binding and hydrolysis: evidence for a conserved subdomain, the A-loop in the ATP-binding cassette
    In Wha Kim
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 45:7605-16. 2006
  6. ncbi request reprint A "silent" polymorphism in the MDR1 gene changes substrate specificity
    Chava Kimchi-Sarfaty
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Science 315:525-8. 2007
  7. ncbi request reprint Multidrug resistance protein 4 (ABCC4)-mediated ATP hydrolysis: effect of transport substrates and characterization of the post-hydrolysis transition state
    Zuben E Sauna
    Laboratory of Cell Biology, Center for Cancer Research, NCI, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892 4256, USA
    J Biol Chem 279:48855-64. 2004
  8. ncbi request reprint Catalytic cycle of ATP hydrolysis by P-glycoprotein: evidence for formation of the E.S reaction intermediate with ATP-gamma-S, a nonhydrolyzable analogue of ATP
    Zuben E Sauna
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 4256, USA
    Biochemistry 46:13787-99. 2007
  9. pmc Mutations define cross-talk between the N-terminal nucleotide-binding domain and transmembrane helix-2 of the yeast multidrug transporter Pdr5: possible conservation of a signaling interface for coupling ATP hydrolysis to drug transport
    Zuben E Sauna
    Laboratory of Cell Biology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892 4256, USA
    J Biol Chem 283:35010-22. 2008
  10. pmc Detection of intracellular ADAMTS13, a secreted zinc-metalloprotease, via flow cytometry
    S Geetha
    Division of Hematology, Laboratory of Hemostasis, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
    Cytometry A 75:675-81. 2009

Collaborators

Detail Information

Publications34

  1. pmc Polymorphisms in the F8 gene and MHC-II variants as risk factors for the development of inhibitory anti-factor VIII antibodies during the treatment of hemophilia a: a computational assessment
    Gouri Shankar Pandey
    Laboratory of Hemostasis, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA
    PLoS Comput Biol 9:e1003066. 2013
    ....
  2. pmc Aptamers as a sensitive tool to detect subtle modifications in therapeutic proteins
    Ran Zichel
    Laboratory of Hemostasis, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, United States of America
    PLoS ONE 7:e31948. 2012
    ..The technique can provide an early warning of structural changes during the manufacturing process that could have consequential outcomes downstream...
  3. pmc Characterization of conformation-sensitive antibodies to ADAMTS13, the von Willebrand cleavage protease
    Zuben E Sauna
    Laboratory of Hemostasis, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, United States of America
    PLoS ONE 4:e6506. 2009
    ..Conformation sensitive antibodies have been used to monitor protein conformation and to decipher the molecular mechanism of proteins as well as to distinguish functional and non-functional mutants...
  4. doi request reprint Understanding the contribution of synonymous mutations to human disease
    Zuben E Sauna
    Laboratory of Hemostasis, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, 29 Lincoln Drive, Bethesda, Maryland 20892, USA
    Nat Rev Genet 12:683-91. 2011
    ..Here we review current understanding of the extent to which synonymous mutations influence disease, the various molecular mechanisms that underlie these effects and the implications for future research and biomedical applications...
  5. ncbi request reprint The conserved tyrosine residues 401 and 1044 in ATP sites of human P-glycoprotein are critical for ATP binding and hydrolysis: evidence for a conserved subdomain, the A-loop in the ATP-binding cassette
    In Wha Kim
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 45:7605-16. 2006
    ..We named this subdomain the "A-loop" (aromatic residue interacting with the adenine ring of ATP)...
  6. ncbi request reprint A "silent" polymorphism in the MDR1 gene changes substrate specificity
    Chava Kimchi-Sarfaty
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    Science 315:525-8. 2007
    ....
  7. ncbi request reprint Multidrug resistance protein 4 (ABCC4)-mediated ATP hydrolysis: effect of transport substrates and characterization of the post-hydrolysis transition state
    Zuben E Sauna
    Laboratory of Cell Biology, Center for Cancer Research, NCI, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892 4256, USA
    J Biol Chem 279:48855-64. 2004
    ....
  8. ncbi request reprint Catalytic cycle of ATP hydrolysis by P-glycoprotein: evidence for formation of the E.S reaction intermediate with ATP-gamma-S, a nonhydrolyzable analogue of ATP
    Zuben E Sauna
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 4256, USA
    Biochemistry 46:13787-99. 2007
    ....
  9. pmc Mutations define cross-talk between the N-terminal nucleotide-binding domain and transmembrane helix-2 of the yeast multidrug transporter Pdr5: possible conservation of a signaling interface for coupling ATP hydrolysis to drug transport
    Zuben E Sauna
    Laboratory of Cell Biology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892 4256, USA
    J Biol Chem 283:35010-22. 2008
    ....
  10. pmc Detection of intracellular ADAMTS13, a secreted zinc-metalloprotease, via flow cytometry
    S Geetha
    Division of Hematology, Laboratory of Hemostasis, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
    Cytometry A 75:675-81. 2009
    ..Flow cytometry is a convenient, efficient, and cost-effective way to measure the expression levels of ADAMTS13...
  11. ncbi request reprint Genomics and the mechanism of P-glycoprotein (ABCB1)
    Zuben E Sauna
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, DHHS, Building 37, 37 Convent Drive, Bethesda, MD 20892 4256, USA
    J Bioenerg Biomembr 39:481-7. 2007
    ..Our results suggest that the power-stroke is provided only after formation of the pre-hydrolysis transition-like (E.S) state during ATP hydrolysis...
  12. pmc Inhibition of multidrug resistance-linked P-glycoprotein (ABCB1) function by 5'-fluorosulfonylbenzoyl 5'-adenosine: evidence for an ATP analogue that interacts with both drug-substrate-and nucleotide-binding sites
    Shinobu Ohnuma
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 4256, USA
    Biochemistry 50:3724-35. 2011
    ..Thus, FSBA is an ATP analogue that interacts with both the drug-binding and ATP-binding sites of P-gp, but fluorosulfonyl-mediated cross-linking is observed only at the NBDs...
  13. pmc Characterization of coding synonymous and non-synonymous variants in ADAMTS13 using ex vivo and in silico approaches
    Nathan C Edwards
    Laboratory of Hemostasis, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, United States of America
    PLoS ONE 7:e38864. 2012
    ....
  14. ncbi request reprint Exploiting reaction intermediates of the ATPase reaction to elucidate the mechanism of transport by P-glycoprotein (ABCB1)
    Zuben E Sauna
    Laboratory of Cell Biology, Center for Cancer Research, NCI, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892 4256, USA
    J Biol Chem 281:26501-11. 2006
    ..This novel approach applies transition state theory to elucidate the mechanism of P-glycoprotein and other ABC transporters and has wider applications in testing cause-effect hypotheses in coupled systems...
  15. ncbi request reprint Silent polymorphisms speak: how they affect pharmacogenomics and the treatment of cancer
    Zuben E Sauna
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH and Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
    Cancer Res 67:9609-12. 2007
    ..We discuss the importance of polymorphisms in drug metabolizing enzymes and transporters in anticancer therapy and suggest that synonymous polymorphisms may play a more significant role than is currently assumed...
  16. ncbi request reprint The A-loop, a novel conserved aromatic acid subdomain upstream of the Walker A motif in ABC transporters, is critical for ATP binding
    Suresh V Ambudkar
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, DHHS, Bethesda, MD 20892 4256, USA
    FEBS Lett 580:1049-55. 2006
    ....
  17. ncbi request reprint Biochemical basis of polyvalency as a strategy for enhancing the efficacy of P-glycoprotein (ABCB1) modulators: stipiamide homodimers separated with defined-length spacers reverse drug efflux with greater efficacy
    Zuben E Sauna
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 4254, USA
    Biochemistry 43:2262-71. 2004
    ..These results suggest that polyvalency could be a useful strategy for the development of more potent Pgp modulators...
  18. pmc Detection of intracellular Factor VIII protein in peripheral blood mononuclear cells by flow cytometry
    Gouri Shankar Pandey
    Laboratory of Hemostasis, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, 29 Lincoln Drive, Bethesda, MD 20892, USA
    Biomed Res Int 2013:793502. 2013
    ..In summary, our data suggest that intracellular FVIII detection in PBMCs of hemophilia A patients can be a rapid and reliable method to detect intracellular FVIII levels...
  19. ncbi request reprint The power of the pump: mechanisms of action of P-glycoprotein (ABCB1)
    Suresh V Ambudkar
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, DHHS, Bethesda, MD 20892 4256, USA
    Eur J Pharm Sci 27:392-400. 2006
    ....
  20. ncbi request reprint The molecular basis of the action of disulfiram as a modulator of the multidrug resistance-linked ATP binding cassette transporters MDR1 (ABCB1) and MRP1 (ABCC1)
    Zuben E Sauna
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4254, USA
    Mol Pharmacol 65:675-84. 2004
    ....
  21. pmc Cyclosporin A impairs the secretion and activity of ADAMTS13 (a disintegrin and metalloprotease with thrombospondin type 1 repeat)
    Klilah Hershko
    Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20982, USA
    J Biol Chem 287:44361-71. 2012
    ..These results altogether provide a novel mechanistic explanation for CsA-induced TTP in transplant patients...
  22. ncbi request reprint Disulfiram is a potent modulator of multidrug transporter Cdr1p of Candida albicans
    Suneet Shukla
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4256, USA
    Biochem Biophys Res Commun 322:520-5. 2004
    ..Collectively these results demonstrate that disulfiram reverses Cdr1p-mediated drug resistance by interaction with both ATP and substrate-binding sites of the transporter and may be useful for antifungal therapy...
  23. doi request reprint Endogenous factor VIII synthesis from the intron 22-inverted F8 locus may modulate the immunogenicity of replacement therapy for hemophilia A
    Gouri Shankar Pandey
    Laboratory of Hemostasis, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA
    Nat Med 19:1318-24. 2013
    ..This algorithm showed statistically significant accuracy in predicting the presence of inhibitors in 25 unrelated individuals with the intron 22 inversion. ..
  24. doi request reprint Plasma derivatives: new products and new approaches
    Zuben E Sauna
    Laboratory of Hemostasis, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
    Biologicals 40:191-5. 2012
    ..Finally, novel technologies and potential products are emerging that utilize synthetic molecules in lieu of replacement proteins obviating the limitations associated with replacement therapies...
  25. pmc A novel way to spread drug resistance in tumor cells: functional intercellular transfer of P-glycoprotein (ABCB1)
    Suresh V Ambudkar
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4256, USA
    Trends Pharmacol Sci 26:385-7. 2005
    ..Non-genetic transfer of the multidrug resistance phenotype raises fascinating questions about the mechanism and regulation of cell-surface membrane-protein-mediated spread of traits...
  26. pmc Selective toxicity of NSC73306 in MDR1-positive cells as a new strategy to circumvent multidrug resistance in cancer
    Joseph A Ludwig
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892, USA
    Cancer Res 66:4808-15. 2006
    ..This article shows that NSC73306 kills cells with intrinsic or acquired P-gp-induced MDR and indirectly acts to eliminate resistance to MDR1 substrates...
  27. ncbi request reprint About a switch: how P-glycoprotein (ABCB1) harnesses the energy of ATP binding and hydrolysis to do mechanical work
    Zuben E Sauna
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Building 37, Room 2120, 37 Convent Drive, Bethesda, MD 20892 4256, USA
    Mol Cancer Ther 6:13-23. 2007
    ..Based on the mutational and biochemical work on Pgp and structural studies with isolated NBDs, we review proposed schemes for the catalytic cycle of ATP hydrolysis and the transport pathway...
  28. doi request reprint Silent (synonymous) SNPs: should we care about them?
    Ryan Hunt
    Laboratory of Hemostasis, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA
    Methods Mol Biol 578:23-39. 2009
    ..These changes can have a significant effect on the function of proteins, change cellular response to therapeutic targets, and often explain the different responses of individual patients to a certain medication...
  29. ncbi request reprint Disulfiram, an old drug with new potential therapeutic uses for human cancers and fungal infections
    Zuben E Sauna
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, DHHS, Bethesda, Maryland 20892 4256, USA
    Mol Biosyst 1:127-34. 2005
    ..This review discusses the molecular mechanism of action of disulfiram and its potential use in the treatment of human cancers and fungal infections...
  30. ncbi request reprint P-glycoprotein: from genomics to mechanism
    Suresh V Ambudkar
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute NIH, 37 Convent Drive, Building 37, Room 1A 09, Bethesda, MD 20892 4254, USA
    Oncogene 22:7468-85. 2003
    ..Understanding of the biology, genetics, and biochemistry of P-gp promises to improve the treatment of cancer and explain the pharmacokinetics of many commonly used drugs...
  31. doi request reprint Building better drugs: developing and regulating engineered therapeutic proteins
    Chava Kimchi-Sarfaty
    Laboratory of Hemostasis, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA Electronic address
    Trends Pharmacol Sci 34:534-48. 2013
    ..This in turn holds promise for more predictable criteria for the licensure of a class of products that are extremely challenging to develop but represent an increasingly important component of modern medical practice. ..
  32. ncbi request reprint Mutational analysis of ABCG2: role of the GXXXG motif
    Orsolya Polgar
    Cancer Therapeutics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, Maryland 20892, USA
    Biochemistry 43:9448-56. 2004
    ..These studies support a hypothesis that the GXXXG motif promotes proper packing of the transmembrane segments in the functional ABCG2 homodimer, although it does not solely arbitrate dimerization...
  33. pmc Identification of sequence-structure RNA binding motifs for SELEX-derived aptamers
    Jan Hoinka
    National Center for Biotechnology Information, NLM, NIH, 8600 Rockville Pike, Bethesda, MD 20894, USA
    Bioinformatics 28:i215-23. 2012
    ..At the same time, the combination of SELEX with novel sequencing technologies is beginning to provide the data that will allow the examination of a variety of properties of the selection process...
  34. ncbi request reprint Importance of the conserved Walker B glutamate residues, 556 and 1201, for the completion of the catalytic cycle of ATP hydrolysis by human P-glycoprotein (ABCB1)
    Zuben E Sauna
    Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 4254, USA
    Biochemistry 41:13989-4000. 2002
    ....