Research Topics
| Jakob ReiserSummaryAffiliation: Food and Drug Administration Country: USA Publications
Research Grants
| Collaborators
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Detail Information
Publications
Cell-specific targeting of lentiviral vectors mediated by fusion proteins derived from Sindbis virus, vesicular stomatitis virus, or avian sarcoma/leukosis virusXian Yang Zhang
Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA
Retrovirology 7:3. 2010....- Lentiviral gene transfer into the dorsal root ganglion of adult ratsHongwei Yu
Department of Anesthesiology, Medical College of Wisconsin, 8100 Watertown Plank Rd, Milwaukee, Wisconsin 53226, USA
Mol Pain 7:63. 2011.... - Lentiviral vectors encoding tetracycline-dependent repressors and transactivators for reversible knockdown of gene expression: a comparative studyKrzysztof Pluta
Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
BMC Biotechnol 7:41. 2007.... - Optimized lentiviral transduction of mouse bone marrow-derived mesenchymal stem cellsDavid M Ricks
Gene Therapy Program, Department of Medicine, LSU Health Sciences Center, New Orleans, LA 70112, USA
Stem Cells Dev 17:441-50. 2008.... - Improved tagging strategy for protein identification in mammalian cellsAgnieszka Bialkowska
Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA
BMC Genomics 6:113. 2005..The tagging strategy enables full-length endogenous proteins in mammalian cells to be expressed as green fluorescent fusion proteins from their authentic promoters... 
Generation of domestic transgenic cloned kittens using lentivirus vectorsMartha C Gómez
Audubon Center for Research of Endangered Species, New Orleans, Louisiana 70131, USA
Cloning Stem Cells 11:167-76. 2009..In summary, we report the birth of transgenic cloned kittens produced by LV vector-mediated transduction of donor cells and confirm that cloned kittens express the EGFP reporter transgene in all body tissues...- Transduction of bone-marrow-derived mesenchymal stem cells by using lentivirus vectors pseudotyped with modified RD114 envelope glycoproteinsXian-Yang Zhang
Gene Therapy Program, Department of Medicine, LSU Health Sciences Center, Tulane Cancer Center, New Orleans, Louisiana 70112, USA
J Virol 78:1219-29. 2004..Taken together, these results suggest that lentivirus pseudotypes bearing alternative Env GPs provide efficient tools for ex vivo modification of human MSCs... - Nuclear transfer of sand cat cells into enucleated domestic cat oocytes is affected by cryopreservation of donor cellsMartha C Gómez
Audubon Center for Research of Endangered Species, New Orleans, Louisiana 70131, USA
Cloning Stem Cells 10:469-83. 2008..Consequently, the number of embryos that cleaved, implanted, and developed to term-gestation and POU5F1 expression in derived blastocysts indirectly was affected... - Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectorsRobert H Kutner
Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, 533 Bolivar Street, New Orleans, Louisiana 70112, USA
Nat Protoc 4:495-505. 2009..The vector production and titration protocol described here can be completed within 8 d... - Altering the tropism of lentiviral vectors through pseudotypingJames Cronin
Gene Therapy Program, Louisiana State University Health Sciences Center, New Orleans, 70112, USA
Curr Gene Ther 5:387-98. 2005..Particular attention is paid to publications of successfully targeting a specific organ or cell types... 
Tight control of transgene expression by lentivirus vectors containing second-generation tetracycline-responsive promotersKrzysztof Pluta
Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center New Orleans, New Orleans, LA 70112, USA
J Gene Med 7:803-17. 2005..CONCLUSIONS: Inducible lentivirus vectors bearing insulators or second-generation TREs will likely prove useful for applications demanding the lowest levels of background expression...- Stable transgene expression in tumors and metastases after transduction with lentiviral vectors based on human immunodeficiency virus type 1Lili Bao
Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
Hum Gene Ther 15:445-56. 2004..These results demonstrate the usefulness of HIV-1-based lentiviral vectors for sustained and stable transgene expression in human lung and breast cancer cell lines in vitro and in vivo... - A comparative analysis of constitutive and cell-specific promoters in the adult mouse hippocampus using lentivirus vector-mediated gene transferHitoshi Kuroda
Gene Therapy Program, Louisiana State University Health Sciences Center, New Orleans, LA, USA
J Gene Med 10:1163-75. 2008..A key advantage of such approaches is that specific brain regions implicated in a particular disease can be independently targeted... - Production of lentiviral vectors in protein-free mediaHitoshi Kuroda
Gene Therapy Program, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA
Curr Protoc Cell Biol . 2011..The vector production and titration protocol described here can be completed within 8 days... - Cellular uptake and lysosomal delivery of galactocerebrosidase tagged with the HIV Tat protein transduction domainXian Yang Zhang
Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA
J Neurochem 104:1055-64. 2008..Therefore, GALC-TMH not only maintained the features of the native GALC protein including enzymatic function, intracellular transport and location, but also displayed more efficient cellular uptake... - Simplified production and concentration of HIV-1-based lentiviral vectors using HYPERFlask vessels and anion exchange membrane chromatographyRobert H Kutner
Gene Therapy Program, Vector Core, Louisiana State University Health Sciences Center, New Orleans, LA, USA
BMC Biotechnol 9:10. 2009..However, despite significant progress, the production and concentration of high-titer, high-quality LV vector stocks is still cumbersome and costly... - Conditional expression of interferon-gamma to enhance host responses to pulmonary bacterial infectionSanbao Ruan
Section of Pulmonary/Critical Care Medicine, LSU Health Sciences Center, Room 3205, 1901 Perdido Street, New Orleans, LA 70112, USA
Pulm Pharmacol Ther 19:251-7. 2006..0 x 10(5) vs 10+0.8 cells/lung, p<0.05). We conclude that local release of IFN-gamma can be selectively activated to enhance neutrophil recruitment and host resistance to bacterial pneumonia... - Simplified lentivirus vector production in protein-free media using polyethylenimine-mediated transfectionHitoshi Kuroda
Louisiana State University Health Sciences Center, New Orleans, 70112, USA
J Virol Methods 157:113-21. 2009..As a consequence, large-scale lentiviral vector stocks can now be generated with fewer batch-to-batch variations and at reduced costs and with less labor compared to the standard protocols... - Potential of mesenchymal stem cells in gene therapy approaches for inherited and acquired diseasesJakob Reiser
LSU Health Sciences Center, Gene Therapy Program, New Orleans, LA, USA
Expert Opin Biol Ther 5:1571-84. 2005..The ultimate dream is to use MSCs in various forms of cellular therapies, as well as genetic tools that can be used to better understand the mechanisms leading to repair and regeneration of damaged or diseased tissues and organs... - Application of lentivirus-mediated RNAi in studying gene function in mammalian tooth developmentYiqiang Song
Division of Developmental Biology, Department of Cell and Molecular Biology, Tulane University, New Orleans, Louisiana 70118, USA
Dev Dyn 235:1334-44. 2006..Our studies have established a reliable and rapid assay that would permit large-scale analysis of gene function in mammalian tooth development... - Small- to large-scale production of lentivirus vectorsMichael P Marino
Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA, USA
Methods Mol Biol 229:43-55. 2003 - Mesenchymal stem cellsXian-Yang Zhang
Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, USA
Methods Mol Biol 229:131-40. 2003
Research Grants
- Delivery of Therapeutic Enzymes and Genes to the CNSJakob Reiser; Fiscal Year: 2004..3. To use lentiviral vectors to transfer the GALC cDNA into the brains of mice and rhesus monkeys affected with GLD to evaluate the capacity of such vectors to correct the GLD defect and histological abnormalities. ..