Jakob Reiser

Summary

Affiliation: Food and Drug Administration
Country: USA

Publications

  1. pmc Targeting of herpes simplex virus 1 thymidine kinase gene sequences into the OCT4 locus of human induced pluripotent stem cells
    Wu Ou
    Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, FDA, Bethesda, Maryland, United States of America
    PLoS ONE 8:e81131. 2013
  2. pmc Cell-specific targeting of lentiviral vectors mediated by fusion proteins derived from Sindbis virus, vesicular stomatitis virus, or avian sarcoma/leukosis virus
    Xian Yang Zhang
    Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA
    Retrovirology 7:3. 2010
  3. pmc Lentiviral gene transfer into the dorsal root ganglion of adult rats
    Hongwei Yu
    Department of Anesthesiology, Medical College of Wisconsin, 8100 Watertown Plank Rd, Milwaukee, Wisconsin 53226, USA
    Mol Pain 7:63. 2011
  4. pmc Lentiviral vectors encoding tetracycline-dependent repressors and transactivators for reversible knockdown of gene expression: a comparative study
    Krzysztof Pluta
    Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
    BMC Biotechnol 7:41. 2007
  5. pmc Optimized lentiviral transduction of mouse bone marrow-derived mesenchymal stem cells
    David M Ricks
    Gene Therapy Program, Department of Medicine, LSU Health Sciences Center, New Orleans, LA 70112, USA
    Stem Cells Dev 17:441-50. 2008
  6. pmc Improved tagging strategy for protein identification in mammalian cells
    Agnieszka Bialkowska
    Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA
    BMC Genomics 6:113. 2005
  7. doi request reprint Generation of domestic transgenic cloned kittens using lentivirus vectors
    Martha C Gómez
    Audubon Center for Research of Endangered Species, New Orleans, Louisiana 70131, USA
    Cloning Stem Cells 11:167-76. 2009
  8. doi request reprint Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors
    Robert H Kutner
    Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, 533 Bolivar Street, New Orleans, Louisiana 70112, USA
    Nat Protoc 4:495-505. 2009
  9. doi request reprint Nuclear transfer of sand cat cells into enucleated domestic cat oocytes is affected by cryopreservation of donor cells
    Martha C Gómez
    Audubon Center for Research of Endangered Species, New Orleans, Louisiana 70131, USA
    Cloning Stem Cells 10:469-83. 2008
  10. pmc Altering the tropism of lentiviral vectors through pseudotyping
    James Cronin
    Gene Therapy Program, Louisiana State University Health Sciences Center, New Orleans, 70112, USA
    Curr Gene Ther 5:387-98. 2005

Research Grants

Collaborators

Detail Information

Publications23

  1. pmc Targeting of herpes simplex virus 1 thymidine kinase gene sequences into the OCT4 locus of human induced pluripotent stem cells
    Wu Ou
    Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, FDA, Bethesda, Maryland, United States of America
    PLoS ONE 8:e81131. 2013
    ..These TK-expressing hiPSC clones remained GCV sensitive for up to 90 days, indicating that TK transgene expression was stable. Possible reasons for our failed attempt to selectively target the OCT4 locus are discussed. ..
  2. pmc Cell-specific targeting of lentiviral vectors mediated by fusion proteins derived from Sindbis virus, vesicular stomatitis virus, or avian sarcoma/leukosis virus
    Xian Yang Zhang
    Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA
    Retrovirology 7:3. 2010
    ....
  3. pmc Lentiviral gene transfer into the dorsal root ganglion of adult rats
    Hongwei Yu
    Department of Anesthesiology, Medical College of Wisconsin, 8100 Watertown Plank Rd, Milwaukee, Wisconsin 53226, USA
    Mol Pain 7:63. 2011
    ....
  4. pmc Lentiviral vectors encoding tetracycline-dependent repressors and transactivators for reversible knockdown of gene expression: a comparative study
    Krzysztof Pluta
    Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
    BMC Biotechnol 7:41. 2007
    ....
  5. pmc Optimized lentiviral transduction of mouse bone marrow-derived mesenchymal stem cells
    David M Ricks
    Gene Therapy Program, Department of Medicine, LSU Health Sciences Center, New Orleans, LA 70112, USA
    Stem Cells Dev 17:441-50. 2008
    ....
  6. pmc Improved tagging strategy for protein identification in mammalian cells
    Agnieszka Bialkowska
    Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA
    BMC Genomics 6:113. 2005
    ..The tagging strategy enables full-length endogenous proteins in mammalian cells to be expressed as green fluorescent fusion proteins from their authentic promoters...
  7. doi request reprint Generation of domestic transgenic cloned kittens using lentivirus vectors
    Martha C Gómez
    Audubon Center for Research of Endangered Species, New Orleans, Louisiana 70131, USA
    Cloning Stem Cells 11:167-76. 2009
    ..In summary, we report the birth of transgenic cloned kittens produced by LV vector-mediated transduction of donor cells and confirm that cloned kittens express the EGFP reporter transgene in all body tissues...
  8. doi request reprint Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors
    Robert H Kutner
    Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center, 533 Bolivar Street, New Orleans, Louisiana 70112, USA
    Nat Protoc 4:495-505. 2009
    ..The vector production and titration protocol described here can be completed within 8 d...
  9. doi request reprint Nuclear transfer of sand cat cells into enucleated domestic cat oocytes is affected by cryopreservation of donor cells
    Martha C Gómez
    Audubon Center for Research of Endangered Species, New Orleans, Louisiana 70131, USA
    Cloning Stem Cells 10:469-83. 2008
    ..Consequently, the number of embryos that cleaved, implanted, and developed to term-gestation and POU5F1 expression in derived blastocysts indirectly was affected...
  10. pmc Altering the tropism of lentiviral vectors through pseudotyping
    James Cronin
    Gene Therapy Program, Louisiana State University Health Sciences Center, New Orleans, 70112, USA
    Curr Gene Ther 5:387-98. 2005
    ..Particular attention is paid to publications of successfully targeting a specific organ or cell types...
  11. ncbi request reprint Tight control of transgene expression by lentivirus vectors containing second-generation tetracycline-responsive promoters
    Krzysztof Pluta
    Gene Therapy Program, Department of Medicine, Louisiana State University Health Sciences Center New Orleans, New Orleans, LA 70112, USA
    J Gene Med 7:803-17. 2005
    ....
  12. pmc Transduction of bone-marrow-derived mesenchymal stem cells by using lentivirus vectors pseudotyped with modified RD114 envelope glycoproteins
    Xian Yang Zhang
    Gene Therapy Program, Department of Medicine, LSU Health Sciences Center, Tulane Cancer Center, New Orleans, Louisiana 70112, USA
    J Virol 78:1219-29. 2004
    ..Taken together, these results suggest that lentivirus pseudotypes bearing alternative Env GPs provide efficient tools for ex vivo modification of human MSCs...
  13. ncbi request reprint Stable transgene expression in tumors and metastases after transduction with lentiviral vectors based on human immunodeficiency virus type 1
    Lili Bao
    Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
    Hum Gene Ther 15:445-56. 2004
    ..These results demonstrate the usefulness of HIV-1-based lentiviral vectors for sustained and stable transgene expression in human lung and breast cancer cell lines in vitro and in vivo...
  14. doi request reprint A comparative analysis of constitutive and cell-specific promoters in the adult mouse hippocampus using lentivirus vector-mediated gene transfer
    Hitoshi Kuroda
    Gene Therapy Program, Louisiana State University Health Sciences Center, New Orleans, LA, USA
    J Gene Med 10:1163-75. 2008
    ..A key advantage of such approaches is that specific brain regions implicated in a particular disease can be independently targeted...
  15. doi request reprint Production of lentiviral vectors in protein-free media
    Hitoshi Kuroda
    Gene Therapy Program, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA
    Curr Protoc Cell Biol . 2011
    ..The vector production and titration protocol described here can be completed within 8 days...
  16. ncbi request reprint Cellular uptake and lysosomal delivery of galactocerebrosidase tagged with the HIV Tat protein transduction domain
    Xian Yang Zhang
    Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA
    J Neurochem 104:1055-64. 2008
    ..Therefore, GALC-TMH not only maintained the features of the native GALC protein including enzymatic function, intracellular transport and location, but also displayed more efficient cellular uptake...
  17. pmc Simplified production and concentration of HIV-1-based lentiviral vectors using HYPERFlask vessels and anion exchange membrane chromatography
    Robert H Kutner
    Gene Therapy Program, Vector Core, Louisiana State University Health Sciences Center, New Orleans, LA, USA
    BMC Biotechnol 9:10. 2009
    ..However, despite significant progress, the production and concentration of high-titer, high-quality LV vector stocks is still cumbersome and costly...
  18. ncbi request reprint Conditional expression of interferon-gamma to enhance host responses to pulmonary bacterial infection
    Sanbao Ruan
    Section of Pulmonary Critical Care Medicine, LSU Health Sciences Center, Room 3205, 1901 Perdido Street, New Orleans, LA 70112, USA
    Pulm Pharmacol Ther 19:251-7. 2006
    ..0 x 10(5) vs 10+0.8 cells/lung, p<0.05). We conclude that local release of IFN-gamma can be selectively activated to enhance neutrophil recruitment and host resistance to bacterial pneumonia...
  19. doi request reprint Simplified lentivirus vector production in protein-free media using polyethylenimine-mediated transfection
    Hitoshi Kuroda
    Louisiana State University Health Sciences Center, New Orleans, 70112, USA
    J Virol Methods 157:113-21. 2009
    ..As a consequence, large-scale lentiviral vector stocks can now be generated with fewer batch-to-batch variations and at reduced costs and with less labor compared to the standard protocols...
  20. pmc Potential of mesenchymal stem cells in gene therapy approaches for inherited and acquired diseases
    Jakob Reiser
    LSU Health Sciences Center, Gene Therapy Program, New Orleans, LA, USA
    Expert Opin Biol Ther 5:1571-84. 2005
    ..The ultimate dream is to use MSCs in various forms of cellular therapies, as well as genetic tools that can be used to better understand the mechanisms leading to repair and regeneration of damaged or diseased tissues and organs...
  21. ncbi request reprint Application of lentivirus-mediated RNAi in studying gene function in mammalian tooth development
    Yiqiang Song
    Division of Developmental Biology, Department of Cell and Molecular Biology, Tulane University, New Orleans, Louisiana 70118, USA
    Dev Dyn 235:1334-44. 2006
    ..Our studies have established a reliable and rapid assay that would permit large-scale analysis of gene function in mammalian tooth development...
  22. ncbi request reprint Small- to large-scale production of lentivirus vectors
    Michael P Marino
    Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA, USA
    Methods Mol Biol 229:43-55. 2003
  23. ncbi request reprint Mesenchymal stem cells
    Xian Yang Zhang
    Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, USA
    Methods Mol Biol 229:131-40. 2003

Research Grants2

  1. Delivery of Therapeutic Enzymes and Genes to the CNS
    Jakob Reiser; Fiscal Year: 2004
    ..3. To use lentiviral vectors to transfer the GALC cDNA into the brains of mice and rhesus monkeys affected with GLD to evaluate the capacity of such vectors to correct the GLD defect and histological abnormalities. ..