Research Topics
Species | Keith PedenSummaryAffiliation: Food and Drug Administration Country: USA Publications
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Detail Information
Publications
Recovery of strains of the polyomavirus SV40 from rhesus monkey kidney cells dating from the 1950s to the early 1960sKeith Peden
Laboratory of Retrovirus Research, Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
Virology 370:63-76. 2008..These results, and those of others, suggest that a limited number of SV40 strains might exist in rhesus macaques in the United States, and thus determining the origin of the SV40 sequences detected in human tumors might be difficult...
Tumors induced in mice by direct inoculation of plasmid DNA expressing both activated H-ras and c-mycLi Sheng-Fowler
Division of Viral Products, OVRR, CBER, FDA, Bethesda, MD 20892, USA
Int J Biol Sci 6:151-62. 2010..Based on these data, the dual-expression plasmid pMSV-T24-H-ras/MSV-c-myc will be used as the positive control to develop a sensitive and quantitative animal assay that can be used to assess the oncogenic activity of DNA...
A quantitative PCR assay for SV40 neutralization adaptable for high-throughput applicationsHaruhiko Murata
Laboratory of DNA Viruses, Division of Viral Products, CBER, FDA, Bethesda, MD 20892, USA
J Virol Methods 162:236-44. 2009..A similar high-throughput approach might be feasible for related polyomaviruses (e.g., BKV and JCV) as well as for other families of viruses...
Identification of a neutralization epitope in the VP1 capsid protein of SV40Haruhiko Murata
Laboratory of DNA Viruses, Division of Viral Products CBER, FDA Bethesda, MD 20892, USA
Virology 381:116-22. 2008..Structural information regarding the neutralization epitope should be useful for clarifying the extent of cross-reactivity exhibited by the humoral immune response towards related primate polyomaviruses (e.g., SV40, BKV, and JCV)...
Patterns of microRNA expression in non-human primate cells correlate with neoplastic development in vitroBelete Teferedegne
Laboratory of DNA Viruses, Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, United States of America
PLoS ONE 5:e14416. 2010..These results may prove to be relevant to the biology of neoplastic development. In addition, one or more of these miRNAs could be biomarkers for the expression of a tumorigenic phenotype...
Heterogeneity of the tumorigenic phenotype expressed by Madin-Darby canine kidney cellsRomelda L Omeir
Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and DrugAdministration, National Institutes of Health, Bethesda, Maryland, USA
Comp Med 61:243-50. 2011..Such data also are useful when considering MDCK cells as a reagent for vaccine manufacture...
Identification of a mutation in the SV40 capsid protein VP1 that influences plaque morphology, vacuolization, and receptor usageHaruhiko Murata
Cancer Prevention Fellowship Program, Office of Preventive Oncology, Division of Cancer Prevention, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
Virology 370:343-51. 2008..This VP1 mutation was identified as a genetic determinant influencing a number of phenotypes associated with SV40-LP(KT) such as plaque morphology, intracellular vacuole formation, and ganglioside receptor usage...
Oncogenicity of DNA in vivo: tumor induction with expression plasmids for activated H-ras and c-mycLi Sheng
Division of Viral Products, OVRR, CBER, FDA, Building 29A, Room 3D08, 29 Lincoln Drive, Bethesda, MD 20892, USA
Biologicals 36:184-97. 2008..Such information provides a possible way of evaluating and estimating the theoretical oncogenic risk posed by residual cell-substrate DNA in vaccines...
Quantitative determination of the infectivity of the proviral DNA of a retrovirus in vitro: Evaluation of methods for DNA inactivationLi Sheng-Fowler
Laboratory of Retrovirus Research, Division of Viral Products, Center for Biologics Research and Evaluation, Food and Drug Administration, Bethesda, MD 20892, USA
Biologicals 37:259-69. 2009..Thus, the potential risk associated with DNA can be substantially reduced by degradation or by chemical inactivation...
Real-time, quantitative PCR assays for the detection of virus-specific DNA in samples with mixed populations of polyomavirusesAchintya Pal
Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
J Virol Methods 135:32-42. 2006..5 and 1 copy of the JCV genome. We suggest that the virus-specific primer/probe sets in this study be considered sufficiently characterized to initiate the quantification of polyomavirus DNA in biological samples...
Plaque purification as a method to mitigate the risk of adventitious-agent contamination in influenza vaccine virus seedsHaruhiko Murata
Laboratory of DNA Viruses, Division of Viral Products, CBER, FDA, Bethesda, MD 20892, USA
Vaccine 29:3155-61. 2011....
Issues associated with residual cell-substrate DNA in viral vaccinesLi Sheng-Fowler
Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drugs Administration, Bethesda, MD 20892, USA
Biologicals 37:190-5. 2009....
Assessing the tumorigenic phenotype of VERO cells in adult and newborn nude miceManu Manohar
Division of Viral Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, Room 1B10, Building 29A, 29 Lincoln Drive, Bethesda, MD 20892, USA
Biologicals 36:65-72. 2008..Higher tumor incidences and shorter tumor latencies suggest that newborn nude mice may be more sensitive than adults in detecting the expression of a tumorigenic phenotype by some VERO cell lines...
Human immunodeficiency virus (HIV) gp41 escape mutants: cross-resistance to peptide inhibitors of HIV fusion and altered receptor activation of gp120Emmanuel Desmezieres
U.S. Food and Drug Administration, Center for Biologics Evaluation and Research, HFM-466, Bldg. 29, Room 532, 8800 Rockville Pike, Bethesda, MD 20892-4555, USA
J Virol 79:4774-81. 2005..The results are discussed in the context of current models of Env-mediated membrane fusion...
WHO informal consultation on the application of molecular methods to assure the quality, safety and efficacy of vaccines, Geneva, Switzerland, 7-8 April 2005Jinho Shin
World Health Organization, Department of Immunizations, Vaccines and Biologicals, Avenue Appia, CH 1211 Geneva, Switzerland
Biologicals 35:63-71. 2007....
