Genomes and Genes
M H Kothary
Affiliation: Food and Drug Administration
- Characterization of the zinc-containing metalloprotease encoded by zpx and development of a species-specific detection method for Enterobacter sakazakiiM H Kothary
MOD 1 Facility, Virulence Mechanisms Branch, Division of Virulence Assessment, OARSA, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, Laurel, MD 20708, USA
Appl Environ Microbiol 73:4142-51. 2007..23. The sequence possesses three well-characterized zinc-binding and active-site motifs present in other bacterial zinc metalloproteases...
- Identification and characterization of Cronobacter iron acquisition systemsC J Grim
CFSAN, FDA, Laurel, Maryland, USA
Appl Environ Microbiol 78:6035-50. 2012..Taken together, these results support the proposition that C. sakazakii and C. malonaticus may be more associated with the human host and C. dublinensis and C. muytjensii with plants...
- Characterization of putative virulence genes on the related RepFIB plasmids harbored by Cronobacter sppA A Franco
Center for Food Safety and Applied Nutrition, U S Food and Drug Administration, Laurel, MD, USA
Appl Environ Microbiol 77:3255-67. 2011..These results support the hypothesis that these plasmids have evolved from a single archetypical plasmid backbone through the cointegration, or deletion, of specific virulence traits in each species...
- The Pathogen-annotated Tracking Resource Network (PATRN) system: a web-based resource to aid food safety, regulatory science, and investigations of foodborne pathogens and diseaseG Gopinath
CFSAN, FDA, Laurel, MD 20708, USA
Food Microbiol 34:303-18. 2013..In summary, PATRN is a web-based integrated platform containing tools for the management, analysis and visualization of data about foodborne pathogens...
- Purification and characterization of a vulnificolysin-like cytolysin produced by Vibrio tubiashiiM H Kothary
Divisions of Virulence Assessment, Center for Food Safety and Applied Nutrition, U S Food and Drug Administration, Washington, DC 20204, USA
Appl Environ Microbiol 67:3707-11. 2001..Twelve of the first 17 N-terminal amino acid residues (Asp-Asp-Tyr-Val-Pro-Val-Val-Glu-Lys-Val-Tyr-Tyr-Ile-Thr-Ser-Ser-Lys) are identical to those of the Vibrio vulnificus cytolysin...
- Two-step purification and partial characterization of a variant of the Vibrio cholerae non-O1 hemolysinB A McCardell
Division of Virulence Assessment, Center for Food Safety and Applied Nutrition, FDA, Washington, DC 20204, USA
FEMS Microbiol Lett 180:177-82. 1999..3, respectively. The N-terminal amino acid sequence was ASPAPANSETNTLPHVAFYI. Purified toxin was cytolytic for Chinese hamster ovary cells and erythrocytes from several animal species...
- Identification of a CHO cell-elongating factor produced by Vibrio cholerae O1B A McCardell
U S Food and Drug Administration, Division of Virulence Assessment, 200 C St SW, Washington, DC 20204, USA
Microb Pathog 29:1-8. 2000..Partly purified Cef did not increase cyclic AMP or prostaglandin E(2)levels in CHO cells which suggests that its mechanism of action differs from that of cholera toxin...
- Isolation and characterization of Vibrio tubiashii outer membrane proteins and determination of a toxR homologJ Jean Gilles Beaubrun
Howard University, Washington, DC 20050, USA
Appl Environ Microbiol 74:907-11. 2008..Results showed the presence of a 38- to 40-kDa OmpU-like protein and ompU gene, a maltoporin-like protein, several novel OMPs, and a regulatory toxR homolog...
- Isolation and characterization of a zinc-containing metalloprotease expressed by Vibrio tubiashiiR B Delston
Division of Microbiological Studies, Center for Food Safety and Applied Nutrition, U S Food and Drug Administration, College Park, MD 20740, USA
Can J Microbiol 49:525-9. 2003..The N-terminal amino acid sequence (Ala-Gln-Ala-Thr-Gly-Thr-Gly- Pro-Gly-Gly-Asn-Gln-Lys-Thr-Gly-Gln- Tyr-Asn-Phe-Gly) has strong homology to other Vibrio proteases...
- Characterization of Vibrio fluvialis-like strains implicated in limp lobster diseaseB D Tall
Center for Food Safety and Applied Nutrition, U S Food and Drug Administration, College Park, Maryland 20740, USA
Appl Environ Microbiol 69:7435-46. 2003..fluvialis-like organisms were responsible for the originally described systemic disease, which is now called limp lobster disease...
- Cpa, the outer membrane protease of Cronobacter sakazakii, activates plasminogen and mediates resistance to serum bactericidal activityA A Franco
Center for Food Safety and Applied Nutrition, U S Food and Drug Administration, Laurel, Maryland, USA
Infect Immun 79:1578-87. 2011..These results strongly suggest that Cpa may be an important virulence factor involved in serum resistance, as well as in the spread and invasion of C. sakazakii...
- Perkinsus marinus extracellular protease modulates survival of Vibrio vulnificus in Eastern oyster (Crassostrea virginica) hemocytesB D Tall
Divisions of Microbiological Studies, Center for Food Safety and Applied Nutrition, Food and Drug Administration, Washington, D C 20204, USA
Appl Environ Microbiol 65:4261-3. 1999..marinus suppresses the vibriocidal activity of oyster hemocytes to effectively eliminate V. vulnificus, potentially leading to conditions favoring higher numbers of vibrios in oyster tissues...