Dennis J Kopecko

Summary

Affiliation: Food and Drug Administration
Country: USA

Publications

  1. pmc Molecular cloning and characterization of genes for Shigella sonnei form I O polysaccharide: proposed biosynthetic pathway and stable expression in a live salmonella vaccine vector
    De Qi Xu
    Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA
    Infect Immun 70:4414-23. 2002
  2. doi request reprint Bacterial delivery of siRNAs: a new approach to solid tumor therapy
    De Qi Xu
    Laboratory of Enteric and Sexually Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD, USA
    Methods Mol Biol 487:161-87. 2009
  3. ncbi request reprint Core-linked LPS expression of Shigella dysenteriae serotype 1 O-antigen in live Salmonella Typhi vaccine vector Ty21a: preclinical evidence of immunogenicity and protection
    De Qi Xu
    Laboratory of Enteric and Sexually Transmitted Diseases, FDA CBER, Bethesda, MD 20892, United States
    Vaccine 25:6167-75. 2007
  4. ncbi request reprint Considerations in the development of live biotherapeutic products for clinical use
    Jennifer J Ross
    Division of Vaccines and Related Products Applications, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration PHS, Rockville, MD, USA
    Curr Issues Mol Biol 10:13-6. 2008
  5. pmc Anthrax protective antigen delivered by Salmonella enterica serovar Typhi Ty21a protects mice from a lethal anthrax spore challenge
    Manuel Osorio
    Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
    Infect Immun 77:1475-82. 2009
  6. doi request reprint Stable expression of Shigella sonnei form I O-polysaccharide genes recombineered into the chromosome of live Salmonella oral vaccine vector Ty21a
    Madushini N Dharmasena
    Laboratory of Enteric and Sexually Transmitted Diseases, U S FDA Center for Biologics Evaluation and Research, NIH Campus Bldg 29, 8800 Rockville Pike, Mail Code HFM440, Bethesda, MD 20892, USA
    Int J Med Microbiol 303:105-13. 2013
  7. pmc Campylobacter jejuni-mediated induction of CC and CXC chemokines and chemokine receptors in human dendritic cells
    Lan Hu
    Laboratory of Enteric and Sexually Transmitted Diseases, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Bethesda, Maryland, USA
    Infect Immun 80:2929-39. 2012
  8. doi request reprint Genetic stability of vaccine strain Salmonella Typhi Ty21a over 25 years
    Dennis J Kopecko
    Laboratory of Enteric and Sexually Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, 29 Lincoln Drive, NIH Campus, Bldg 29 420, HFM440, Bethesda, MD 20892, USA
    Int J Med Microbiol 299:233-46. 2009
  9. ncbi request reprint Ca2+ release from host intracellular stores and related signal transduction during Campylobacter jejuni 81-176 internalization into human intestinal cells
    Lan Hu
    Laboratory of Enteric and Sexually Transmitted Diseases, FDA Center for Biologics Evaluation and Research, 29 Lincoln Drive, Bldg 29 420 HFM440, Bethesda, MD 20892, USA
    Microbiology 151:3097-105. 2005
  10. doi request reprint Effects of plasmid-based Stat3-specific short hairpin RNA and GRIM-19 on PC-3M tumor cell growth
    Ling Zhang
    Prostate Diseases Prevention and Treatment Research Center and Department of Pathophysiology, School of Basic Medicine, Jilin University, Changchun, PR China
    Clin Cancer Res 14:559-68. 2008

Collaborators

  • Jennifer J Ross
  • Guido Dietrich
  • Manuel Osorio
  • De Qi Xu
  • Lan Hu
  • Ling Zhang
  • Madushini N Dharmasena
  • Tint T Wai
  • Lifang Gao
  • Yueting Shao
  • John O Cisar
  • Shu ichi Nakayama
  • Brock W Hanisch
  • Mechelle D Bray
  • Yansheng Geng
  • Lijing Zhao
  • Baofeng Guo
  • Guimiao Lin
  • Hao Yu
  • Jiadi Hu
  • Yang Li
  • Dhananjaya V Kalvakolanu
  • Kun Ji
  • Xuejian Zhao
  • Richard B Raybourne
  • Haruo Watanabe
  • Ryu Ichiro Tanaka
  • Akira Kushiro
  • Takashi Asahara
  • Donald H Burr
  • Nicholas Ambulos Jr

Detail Information

Publications11

  1. pmc Molecular cloning and characterization of genes for Shigella sonnei form I O polysaccharide: proposed biosynthetic pathway and stable expression in a live salmonella vaccine vector
    De Qi Xu
    Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA
    Infect Immun 70:4414-23. 2002
    ..sonnei challenge, thereby supporting the promise of live, oral vaccines for the prevention of shigellosis...
  2. doi request reprint Bacterial delivery of siRNAs: a new approach to solid tumor therapy
    De Qi Xu
    Laboratory of Enteric and Sexually Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD, USA
    Methods Mol Biol 487:161-87. 2009
    ..This chapter describes the use of attenuated bacteria as tumor-targeting delivery systems for cancer therapy...
  3. ncbi request reprint Core-linked LPS expression of Shigella dysenteriae serotype 1 O-antigen in live Salmonella Typhi vaccine vector Ty21a: preclinical evidence of immunogenicity and protection
    De Qi Xu
    Laboratory of Enteric and Sexually Transmitted Diseases, FDA CBER, Bethesda, MD 20892, United States
    Vaccine 25:6167-75. 2007
    ..These data further demonstrate the utility of S. Typhi Ty21a as a live, bacterial vaccine delivery system for heterologous O-antigens, supporting the promise of a bifunctional oral vaccine for prevention of shigellosis and typhoid fever...
  4. ncbi request reprint Considerations in the development of live biotherapeutic products for clinical use
    Jennifer J Ross
    Division of Vaccines and Related Products Applications, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration PHS, Rockville, MD, USA
    Curr Issues Mol Biol 10:13-6. 2008
    ..S. for a specific clinical use. Product information is an important component of an IND to support a proposed clinical study...
  5. pmc Anthrax protective antigen delivered by Salmonella enterica serovar Typhi Ty21a protects mice from a lethal anthrax spore challenge
    Manuel Osorio
    Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
    Infect Immun 77:1475-82. 2009
    ..anthracis 7702. The ultimate goal is a temperature-stable, safe, oral human vaccine against anthrax infection that can be self-administered in a few doses over a short period of time...
  6. doi request reprint Stable expression of Shigella sonnei form I O-polysaccharide genes recombineered into the chromosome of live Salmonella oral vaccine vector Ty21a
    Madushini N Dharmasena
    Laboratory of Enteric and Sexually Transmitted Diseases, U S FDA Center for Biologics Evaluation and Research, NIH Campus Bldg 29, 8800 Rockville Pike, Mail Code HFM440, Bethesda, MD 20892, USA
    Int J Med Microbiol 303:105-13. 2013
    ..sonnei...
  7. pmc Campylobacter jejuni-mediated induction of CC and CXC chemokines and chemokine receptors in human dendritic cells
    Lan Hu
    Laboratory of Enteric and Sexually Transmitted Diseases, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Bethesda, Maryland, USA
    Infect Immun 80:2929-39. 2012
    ..These results suggest that DCs play a significant role in the initiation and modulation of the inflammatory response by enlisting monocytes, neutrophils, and T lymphocytes during human intestinal infection with Campylobacter...
  8. doi request reprint Genetic stability of vaccine strain Salmonella Typhi Ty21a over 25 years
    Dennis J Kopecko
    Laboratory of Enteric and Sexually Transmitted Diseases, Center for Biologics Evaluation and Research, Food and Drug Administration, 29 Lincoln Drive, NIH Campus, Bldg 29 420, HFM440, Bethesda, MD 20892, USA
    Int J Med Microbiol 299:233-46. 2009
    ..Finally, we have confirmed the correlation of these genetic mutations with the expected phenotypic attenuations for the seed lots used in vaccine manufacture over 25 years...
  9. ncbi request reprint Ca2+ release from host intracellular stores and related signal transduction during Campylobacter jejuni 81-176 internalization into human intestinal cells
    Lan Hu
    Laboratory of Enteric and Sexually Transmitted Diseases, FDA Center for Biologics Evaluation and Research, 29 Lincoln Drive, Bldg 29 420 HFM440, Bethesda, MD 20892, USA
    Microbiology 151:3097-105. 2005
    ..jejuni invasion. These results demonstrate that C. jejuni induces the mobilization of Ca(2+) from host intracellular stores, which is an essential step in the invasion of intestinal cells by this pathogen...
  10. doi request reprint Effects of plasmid-based Stat3-specific short hairpin RNA and GRIM-19 on PC-3M tumor cell growth
    Ling Zhang
    Prostate Diseases Prevention and Treatment Research Center and Department of Pathophysiology, School of Basic Medicine, Jilin University, Changchun, PR China
    Clin Cancer Res 14:559-68. 2008
    ..To enhance the therapeutic efficacy of Stat3-specific shRNA, we applied a combination treatment involving gene associated with retinoid-IFN-induced mortality 19 (GRIM-19), another inhibitor of STAT3, along with shRNA...
  11. ncbi request reprint Activation of hilA expression at low pH requires the signal sensor CpxA, but not the cognate response regulator CpxR, in Salmonella enterica serovar Typhimurium
    Shu ichi Nakayama
    Department of Bacteriology, National Institute of Infectious Diseases, Toyama, 1 23 1, Shinjuku ku, Tokyo 162 8640, Japan
    Microbiology 149:2809-17. 2003
    ..Further, the results show that this CpxA-mediated activation does not require its putative cognate response regulator, CpxR. This suggests that CpxA may interact with regulator(s) other than CpxR to achieve activation at low pH...