Karen L Elkins

Summary

Affiliation: Food and Drug Administration
Country: USA

Publications

  1. pmc In vivo clearance of an intracellular bacterium, Francisella tularensis LVS, is dependent on the p40 subunit of interleukin-12 (IL-12) but not on IL-12 p70
    Karen L Elkins
    Laboratory of Mycobacteria, Division of Bacterial, Parasitic, and Allergenic Products, CBER FDA, Rockville, Maryland 20852, USA
    Infect Immun 70:1936-48. 2002
  2. ncbi request reprint Francisella novicida LPS has greater immunobiological activity in mice than F. tularensis LPS, and contributes to F. novicida murine pathogenesis
    Tara L Kieffer
    Laboratory of Mycobacterial Diseases and Cellular Immunity, Division of Bacterial and Parasitic Products, CBER FDA, 1401 Rockville Pike, HFM 431, Bethesda, Rockville, MD 20852, USA
    Microbes Infect 5:397-403. 2003
  3. ncbi request reprint Francisella: a little bug hits the big time
    Karen L Elkins
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, CBER FDA, 1401 Rockville Pike, HFM 431, Rockville, MD 20852, USA
    Expert Rev Vaccines 2:735-8. 2003
  4. ncbi request reprint Innate and adaptive immunity to Francisella
    Karen L Elkins
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic, and Allergenic Products, CBER U S FDA, Bethesda, Maryland, USA
    Ann N Y Acad Sci 1105:284-324. 2007
  5. doi request reprint Survival of secondary lethal systemic Francisella LVS challenge depends largely on interferon gamma
    Karen L Elkins
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic, and Allergenic Products, CBER FDA, HFM 431, Rockville, MD 20852, USA
    Microbes Infect 12:28-36. 2010
  6. doi request reprint NK cells activated in vivo by bacterial DNA control the intracellular growth of Francisella tularensis LVS
    Karen L Elkins
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic, and Allergenic Products, CBER FDA, 1401 Rockville Pike, HFM 431, Rockville, MD 20852, USA
    Microbes Infect 11:49-56. 2009
  7. pmc T cells from lungs and livers of Francisella tularensis-immune mice control the growth of intracellular bacteria
    Carmen M Collazo
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Rockville, Maryland 20852, USA
    Infect Immun 77:2010-21. 2009
  8. ncbi request reprint Differential requirements by CD4+ and CD8+ T cells for soluble and membrane TNF in control of Francisella tularensis live vaccine strain intramacrophage growth
    Siobhán C Cowley
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Research and Evaluation, US Food and Drug Administration, Rockville, MD 20852, USA
    J Immunol 179:7709-19. 2007
  9. ncbi request reprint Lung CD4-CD8- double-negative T cells are prominent producers of IL-17A and IFN-gamma during primary respiratory murine infection with Francisella tularensis live vaccine strain
    Siobhán C Cowley
    Division of Bacterial, Parasitic and Allergenic Products, Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Rockville, MD 20852, USA
    J Immunol 184:5791-801. 2010
  10. ncbi request reprint CD4+ T cells mediate IFN-gamma-independent control of Mycobacterium tuberculosis infection both in vitro and in vivo
    Siobhán C Cowley
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research Food and Drug Administration, Rockville, MD 20852, USA
    J Immunol 171:4689-99. 2003

Collaborators

Detail Information

Publications32

  1. pmc In vivo clearance of an intracellular bacterium, Francisella tularensis LVS, is dependent on the p40 subunit of interleukin-12 (IL-12) but not on IL-12 p70
    Karen L Elkins
    Laboratory of Mycobacteria, Division of Bacterial, Parasitic, and Allergenic Products, CBER FDA, Rockville, Maryland 20852, USA
    Infect Immun 70:1936-48. 2002
    ..Instead, an additional mechanism dependent on the IL-12 p40 protein, either alone or in another complex such as the newly discovered heterodimer IL-23, appears to be responsible for actual clearance of this intracellular bacterium...
  2. ncbi request reprint Francisella novicida LPS has greater immunobiological activity in mice than F. tularensis LPS, and contributes to F. novicida murine pathogenesis
    Tara L Kieffer
    Laboratory of Mycobacterial Diseases and Cellular Immunity, Division of Bacterial and Parasitic Products, CBER FDA, 1401 Rockville Pike, HFM 431, Bethesda, Rockville, MD 20852, USA
    Microbes Infect 5:397-403. 2003
    ..novicida LPS to stimulate the production of proinflammatory cytokines including TNF-alpha likely contributes to the increased virulence for mice of F. novicida compared to F. tularensis LVS...
  3. ncbi request reprint Francisella: a little bug hits the big time
    Karen L Elkins
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, CBER FDA, 1401 Rockville Pike, HFM 431, Rockville, MD 20852, USA
    Expert Rev Vaccines 2:735-8. 2003
  4. ncbi request reprint Innate and adaptive immunity to Francisella
    Karen L Elkins
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic, and Allergenic Products, CBER U S FDA, Bethesda, Maryland, USA
    Ann N Y Acad Sci 1105:284-324. 2007
    ..Thus a number of known proinflammatory and Th-1 T cell related components of the immune system combat this virulent bacterium; no doubt others remain to be discovered...
  5. doi request reprint Survival of secondary lethal systemic Francisella LVS challenge depends largely on interferon gamma
    Karen L Elkins
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic, and Allergenic Products, CBER FDA, HFM 431, Rockville, MD 20852, USA
    Microbes Infect 12:28-36. 2010
    ..Thus successful priming of protective LVS-immune T cells, as well as complete expression of protection against Francisella during secondary challenge, depends heavily on IFN-gamma...
  6. doi request reprint NK cells activated in vivo by bacterial DNA control the intracellular growth of Francisella tularensis LVS
    Karen L Elkins
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic, and Allergenic Products, CBER FDA, 1401 Rockville Pike, HFM 431, Rockville, MD 20852, USA
    Microbes Infect 11:49-56. 2009
    ..Thus, NK cells contribute to DNA-mediated protection by production of cytokines including IFN-gamma and TNF-alpha, resulting in nitric oxide production and control of intracellular Francisella replication...
  7. pmc T cells from lungs and livers of Francisella tularensis-immune mice control the growth of intracellular bacteria
    Carmen M Collazo
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Rockville, Maryland 20852, USA
    Infect Immun 77:2010-21. 2009
    ..Thus, our results indicate functional similarities between immune T cells residing in spleens, livers, and lungs of LVS-immune mice...
  8. ncbi request reprint Differential requirements by CD4+ and CD8+ T cells for soluble and membrane TNF in control of Francisella tularensis live vaccine strain intramacrophage growth
    Siobhán C Cowley
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Research and Evaluation, US Food and Drug Administration, Rockville, MD 20852, USA
    J Immunol 179:7709-19. 2007
    ....
  9. ncbi request reprint Lung CD4-CD8- double-negative T cells are prominent producers of IL-17A and IFN-gamma during primary respiratory murine infection with Francisella tularensis live vaccine strain
    Siobhán C Cowley
    Division of Bacterial, Parasitic and Allergenic Products, Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Rockville, MD 20852, USA
    J Immunol 184:5791-801. 2010
    ....
  10. ncbi request reprint CD4+ T cells mediate IFN-gamma-independent control of Mycobacterium tuberculosis infection both in vitro and in vivo
    Siobhán C Cowley
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research Food and Drug Administration, Rockville, MD 20852, USA
    J Immunol 171:4689-99. 2003
    ..These findings demonstrate that CD4(+) T cells possess IFN-gamma-independent mechanisms that can limit the growth of an intracellular pathogen and are dominant in secondary responses to M. tuberculosis...
  11. ncbi request reprint Innate and adaptive immune responses to an intracellular bacterium, Francisella tularensis live vaccine strain
    Karen L Elkins
    Laboratory of Mycobacteria, Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, 1401 Rockville Pike, HFM 431, Rockville, MD 20852, USA
    Microbes Infect 5:135-42. 2003
    ..Here we review this infection model, which provides a convenient means of studying protective immune mechanisms not only for Francisella, but also for the large and important class of intracellular pathogens...
  12. doi request reprint The membrane form of tumor necrosis factor is sufficient to mediate partial innate immunity to Francisella tularensis live vaccine strain
    Siobhán C Cowley
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Research and Evaluation, US Food and Drug Administration, Rockville, MD 20852, USA
    J Infect Dis 198:284-92. 2008
    ..Thus, memTNF partially functions to regulate chemokine expression, cell recruitment, and nitric oxide production during primary LVS infection and protects against the induction of apoptosis observed in TNF KO mice...
  13. pmc Development of functional and molecular correlates of vaccine-induced protection for a model intracellular pathogen, F. tularensis LVS
    Roberto De Pascalis
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Rockville, Maryland, United States of America
    PLoS Pathog 8:e1002494. 2012
    ..The overall approach may be applicable to intracellular pathogens in general...
  14. pmc T-bet regulates immunity to Francisella tularensis live vaccine strain infection, particularly in lungs
    Amanda A Melillo
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Rockville, Maryland, USA
    Infect Immun 82:1477-90. 2014
    ..Taken together, T-bet is important in host resistance to primary LVS infection and i.n. secondary challenge. Thus, T-bet represents a true, useful correlate for immunity to LVS. ..
  15. pmc CD4-CD8- T cells control intracellular bacterial infections both in vitro and in vivo
    Siobhán C Cowley
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Rockville, MD 20852, USA
    J Exp Med 202:309-19. 2005
    ..tb. or F. tularensis LVS, and then acquire a memory cell phenotype. Thus, CD4(-)CD8(-) T cells have a role in the control of intracellular infection and may contribute to successful vaccination...
  16. pmc Diverse myeloid and lymphoid cell subpopulations produce gamma interferon during early innate immune responses to Francisella tularensis live vaccine strain
    Roberto De Pascalis
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Research and Evaluation, US FDA, 1401 Rockville Pike, HFM 431, Rockville, Maryland 20852, USA
    Infect Immun 76:4311-21. 2008
    ....
  17. pmc Multiple T cell subsets control Francisella tularensis LVS intracellular growth without stimulation through macrophage interferon gamma receptors
    Siobhán C Cowley
    Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, MD 20852, USA
    J Exp Med 198:379-89. 2003
    ....
  18. doi request reprint Generation of protection against Francisella novicida in mice depends on the pathogenicity protein PdpA, but not PdpC or PdpD
    Alicia Y Chou
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, 1401 Rockville Pike, HFM 431, Rockville, MD 20852, USA
    Microbes Infect 15:816-27. 2013
    ..novicida or LVS. Taken together, these results suggest that successful priming of Th1 cells, and protection against lethal challenge, depends on expression of PdpA. ..
  19. pmc IL-12Rβ2 is critical for survival of primary Francisella tularensis LVS infection
    Amanda A Melillo
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Rockville, Maryland 20852, USA
    J Leukoc Biol 93:657-67. 2013
    ..However, IL-12Rβ2 is important in parenteral and mucosal host resistance to primary LVS infection and in the ability of WT mice to clear LVS infection and serves to restrict liver damage...
  20. ncbi request reprint Myeloid differentiation factor-88 (MyD88) is essential for control of primary in vivo Francisella tularensis LVS infection, but not for control of intra-macrophage bacterial replication
    Carmen M Collazo
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, MD 20852, USA
    Microbes Infect 8:779-90. 2006
    ..Thus MyD88 is essential for innate host resistance to LVS infection, but is not required for macrophage control of intracellular bacterial growth...
  21. pmc Interleukin-6 is essential for primary resistance to Francisella tularensis live vaccine strain infection
    Sherry L Kurtz
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Rockville, MD, USA
    Infect Immun 81:585-97. 2013
    ..Taken together, IL-6 is an integral part of a successful immune response to primary LVS infection, but its exact role in precipitating adaptive immunity remains elusive...
  22. pmc Epicutaneous model of community-acquired Staphylococcus aureus skin infections
    Ranjani Prabhakara
    Center for Biologics Evaluation and Research, FDA, Bethesda, Maryland, USA
    Infect Immun 81:1306-15. 2013
    ..In vivo neutrophil depletion demonstrated that neutrophils play a protective role in preventing bacterial dissemination and fatal invasive infection...
  23. doi request reprint Measurement of macrophage-mediated killing of intracellular bacteria, including Francisella and mycobacteria
    Karen L Elkins
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Division of Bacterial, Parasitic, and Allergenic Products, CBER U S FDA, Rockville, Maryland, USA
    Curr Protoc Immunol . 2011
    ....
  24. ncbi request reprint The CXC chemokine murine monokine induced by IFN-gamma (CXC chemokine ligand 9) is made by APCs, targets lymphocytes including activated B cells, and supports antibody responses to a bacterial pathogen in vivo
    Matthew K Park
    Inflammation Biology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 169:1433-43. 2002
    ....
  25. ncbi request reprint Prime-boost immunization with DNA and modified vaccinia virus ankara vectors expressing herpes simplex virus-2 glycoprotein D elicits greater specific antibody and cytokine responses than DNA vaccine alone
    Clement A Meseda
    Laboratory of DNA Viruses, Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, 1401 Rockville Pike, Bethesda, MD 20852, USA
    J Infect Dis 186:1065-73. 2002
    ....
  26. pmc Analysis of the Fc gamma receptor-dependent component of neutralization measured by anthrax toxin neutralization assays
    Anita Verma
    Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
    Clin Vaccine Immunol 16:1405-12. 2009
    ..These findings should be considered when interpreting anthrax toxin neutralization assay output...
  27. pmc Immunity to francisella
    Siobhán C Cowley
    Center for Biologics Evaluation and Research, U S Food and Drug Administration Bethesda, MD, USA
    Front Microbiol 2:26. 2011
    ..Overall, recent data profile a pathogen that is adept at subverting host immune responses, but susceptible to many elements of the immune system's antimicrobial arsenal...
  28. ncbi request reprint Immunologic consequences of Francisella tularensis live vaccine strain infection: role of the innate immune response in infection and immunity
    Leah E Cole
    Department of Microbiology and Immunology, University of Maryland, Baltimore, School of Medicine, Baltimore, MD 21201, USA
    J Immunol 176:6888-99. 2006
    ....
  29. pmc Toll-like receptor 2-mediated signaling requirements for Francisella tularensis live vaccine strain infection of murine macrophages
    Leah E Cole
    Department of Microbiology and Immunology, University of Maryland, Baltimore, 660 West Redwood Street, Room 324, Baltimore, MD 21201, USA
    Infect Immun 75:4127-37. 2007
    ..Collectively, these data indicate that the primary macrophage response to F. tularensis LVS is overwhelmingly TLR2 dependent, requires de novo bacterial protein synthesis, and is independent of intracellular F. tularensis replication...
  30. pmc Macrophage proinflammatory response to Francisella tularensis live vaccine strain requires coordination of multiple signaling pathways
    Leah E Cole
    Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    J Immunol 180:6885-91. 2008
    ....
  31. pmc A Francisella tularensis pathogenicity island required for intramacrophage growth
    Francis E Nano
    Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada
    J Bacteriol 186:6430-6. 2004
    ..7-kb stretch of the FPI is 26.6%, which is 6.6% below the average G+C content of the F. tularensis genome. This extremely low G+C content suggests that the DNA was imported from a microbe with a very low G+C-containing chromosome...
  32. pmc The Francisella pathogenicity island protein PdpD is required for full virulence and associates with homologues of the type VI secretion system
    Jagjit S Ludu
    Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC V8W 3P6, Canada
    J Bacteriol 190:4584-95. 2008
    ..Finally, deletions of FPI genes encoding proteins that are homologues of known components of type VI secretion systems abolished the altered distribution of IglC and the outer membrane localization of PdpD...