Huizhong Chen

Summary

Affiliation: Food and Drug Administration
Country: USA

Publications

  1. doi request reprint Functional role of Trp-105 of Enterococcus faecalis azoreductase (AzoA) as resolved by structural and mutational analysis
    Huizhong Chen
    Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079 9502, USA
    Microbiology 154:2659-67. 2008
  2. doi request reprint Decolorization of water and oil-soluble azo dyes by Lactobacillus acidophilus and Lactobacillus fermentum
    Huizhong Chen
    Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079 9502, USA
    J Ind Microbiol Biotechnol 36:1459-66. 2009
  3. ncbi request reprint Isolation of extremely AT-rich genomic DNA and analysis of genes encoding carbohydrate-degrading enzymes from Orpinomyces sp. strain PC-2
    Huizhong Chen
    Division of Microbiology, National Center for Toxicological Research, U S FDA, Jefferson, AR, 72079, USA
    Curr Microbiol 53:396-400. 2006
  4. ncbi request reprint High level expression and characterization of the cyclophilin B gene from the anaerobic fungus Orpinomyces sp. strain PC-2
    Huizhong Chen
    Division of Microbiology, National Center for Toxicological Research, U S FDA, 3900 NCTR Rd, Jefferson, AR 72079, USA
    Protein Pept Lett 13:727-32. 2006
  5. pmc Identification and molecular characterization of a novel flavin-free NADPH preferred azoreductase encoded by azoB in Pigmentiphaga kullae K24
    Huizhong Chen
    Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079 9502, USA
    BMC Biochem 11:13. 2010
  6. doi request reprint Probing the NADH- and Methyl Red-binding site of a FMN-dependent azoreductase (AzoA) from Enterococcus faecalis
    Jinhui Feng
    Division of Microbiology, National Center for Toxicological Research, U S Food and Drug Administration, Jefferson, AR 72079, United States
    Arch Biochem Biophys 520:99-107. 2012
  7. pmc Anaerobic metabolism of 1-amino-2-naphthol-based azo dyes (Sudan dyes) by human intestinal microflora
    Haiyan Xu
    Division of Microbiology, National Center for Toxicological Research, U S FDA, 3900 NCTR Rd, Jefferson, AR 72079 9502, USA
    Appl Environ Microbiol 73:7759-62. 2007
  8. doi request reprint Sudan azo dyes and Para Red degradation by prevalent bacteria of the human gastrointestinal tract
    Haiyan Xu
    Division of Microbiology, National Center for Toxicological Research, U S Food and Drug Administration, Jefferson, AR 72079 9502, USA
    Anaerobe 16:114-9. 2010
  9. doi request reprint Effects of Orange II and Sudan III azo dyes and their metabolites on Staphylococcus aureus
    Hongmiao Pan
    Division of Microbiology, National Center for Toxicological Research, US FDA, 3900 NCTR Rd, Jefferson, AR 72079 9502, USA
    J Ind Microbiol Biotechnol 38:1729-38. 2011
  10. doi request reprint Metabolism of azo dyes by human skin microbiota
    Robin L Stingley
    Office of Scientific Coordination, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079 9502, USA
    J Med Microbiol 59:108-14. 2010

Collaborators

Detail Information

Publications22

  1. doi request reprint Functional role of Trp-105 of Enterococcus faecalis azoreductase (AzoA) as resolved by structural and mutational analysis
    Huizhong Chen
    Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079 9502, USA
    Microbiology 154:2659-67. 2008
    ..2, 30.6 and 8.2-fold, respectively). However, these mutated enzymes maintained K(m) values similar to the wild-type enzyme. This study provides an insight into the catalytic properties of AzoA in FMN stabilization and enzyme activity...
  2. doi request reprint Decolorization of water and oil-soluble azo dyes by Lactobacillus acidophilus and Lactobacillus fermentum
    Huizhong Chen
    Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079 9502, USA
    J Ind Microbiol Biotechnol 36:1459-66. 2009
    ..Aniline and o-toluidine (2-methylaniline), both potentially carcinogenic aromatic amines, were metabolites of Sudan III and IV, respectively...
  3. ncbi request reprint Isolation of extremely AT-rich genomic DNA and analysis of genes encoding carbohydrate-degrading enzymes from Orpinomyces sp. strain PC-2
    Huizhong Chen
    Division of Microbiology, National Center for Toxicological Research, U S FDA, Jefferson, AR, 72079, USA
    Curr Microbiol 53:396-400. 2006
    ....
  4. ncbi request reprint High level expression and characterization of the cyclophilin B gene from the anaerobic fungus Orpinomyces sp. strain PC-2
    Huizhong Chen
    Division of Microbiology, National Center for Toxicological Research, U S FDA, 3900 NCTR Rd, Jefferson, AR 72079, USA
    Protein Pept Lett 13:727-32. 2006
    ..The overall architecture of the CypB molecule is very similar to that of human CypB...
  5. pmc Identification and molecular characterization of a novel flavin-free NADPH preferred azoreductase encoded by azoB in Pigmentiphaga kullae K24
    Huizhong Chen
    Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079 9502, USA
    BMC Biochem 11:13. 2010
    ..In this study, we report the identification of a novel flavin-free NADPH preferred azoreductase encoded by azoB in Pigmentiphaga kullae K24...
  6. doi request reprint Probing the NADH- and Methyl Red-binding site of a FMN-dependent azoreductase (AzoA) from Enterococcus faecalis
    Jinhui Feng
    Division of Microbiology, National Center for Toxicological Research, U S Food and Drug Administration, Jefferson, AR 72079, United States
    Arch Biochem Biophys 520:99-107. 2012
    ....
  7. pmc Anaerobic metabolism of 1-amino-2-naphthol-based azo dyes (Sudan dyes) by human intestinal microflora
    Haiyan Xu
    Division of Microbiology, National Center for Toxicological Research, U S FDA, 3900 NCTR Rd, Jefferson, AR 72079 9502, USA
    Appl Environ Microbiol 73:7759-62. 2007
    ..These data indicate that human intestinal bacteria are able to reduce Sudan dyes to form potentially carcinogenic aromatic amines...
  8. doi request reprint Sudan azo dyes and Para Red degradation by prevalent bacteria of the human gastrointestinal tract
    Haiyan Xu
    Division of Microbiology, National Center for Toxicological Research, U S Food and Drug Administration, Jefferson, AR 72079 9502, USA
    Anaerobe 16:114-9. 2010
    ..faecalis were isolated and identified by HPLC and LC/ESI-MS analyses and compared with authentic standards. Thus it appears that the ability to reduce Sudan dyes and Para Red except Sudan II is common among bacteria in the human colon...
  9. doi request reprint Effects of Orange II and Sudan III azo dyes and their metabolites on Staphylococcus aureus
    Hongmiao Pan
    Division of Microbiology, National Center for Toxicological Research, US FDA, 3900 NCTR Rd, Jefferson, AR 72079 9502, USA
    J Ind Microbiol Biotechnol 38:1729-38. 2011
    ..p-Phenylenediamine exhibited a synergistic effect with 1-amino-2-naphthol on cell growth inhibition. All of the dye metabolites had no significant effects on cell viability of the bacterium...
  10. doi request reprint Metabolism of azo dyes by human skin microbiota
    Robin L Stingley
    Office of Scientific Coordination, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079 9502, USA
    J Med Microbiol 59:108-14. 2010
    ..delphini, Staph. sciuri subsp. sciuri and Staphylococcus auricularis. The data presented in this report will be useful in the risk assessment process for evaluation of public exposure to products containing these dyes...
  11. ncbi request reprint Molecular cloning, overexpression, purification, and characterization of an aerobic FMN-dependent azoreductase from Enterococcus faecalis
    Huizhong Chen
    Division of Microbiology, National Center for Toxicological Research, US FDA, 3900 NCTR Rd, Jefferson, AR 72079 9502, USA
    Protein Expr Purif 34:302-10. 2004
    ..AzoA is the first aerobic azoreductase to be identified and characterized from human intestinal gram-positive bacteria...
  12. doi request reprint Evaluation of impact of exposure of Sudan azo dyes and their metabolites on human intestinal bacteria
    Hongmiao Pan
    Division of Microbiology, National Center for Toxicological Research, U S Food and Drug Administration, Jefferson, AR 72079, USA
    Anaerobe 18:445-53. 2012
    ..The present results suggested that Sudan azo dyes and their metabolites potentially affect the human intestinal bacterial ecology by selectively inhibiting some bacterial species, which may have an adverse effect on human health...
  13. ncbi request reprint Biochemical and molecular characterization of an azoreductase from Staphylococcus aureus, a tetrameric NADPH-dependent flavoprotein
    Huizhong Chen
    Division of Microbiology, National Center for Toxicological Research, US FDA, 3900 NCTR Road, Jefferson, AR 72079 9502, USA
    Microbiology 151:1433-41. 2005
    ..4 microM min(-1) (mg protein)(-1). Azo1 was also able to metabolize Orange II, Amaranth, Ponceau BS and Ponceau S azo dyes. Azo1 represents the first azoreductase to be identified and characterized from human skin microflora...
  14. pmc Identification of the enzyme responsible for N-acetylation of norfloxacin by Microbacterium sp. Strain 4N2-2
    Dae Wi Kim
    Divisions of Microbiology, U S Food and Drug Administration, Jefferson, Arkansas, USA
    Appl Environ Microbiol 79:314-21. 2013
    ..The results suggest that environmental bacteria whose enzymes modify fluoroquinolones may be able to survive in the presence of low fluoroquinolone concentrations...
  15. doi request reprint Azoreductase in Staphylococcus aureus
    Wen Zou
    National Center for Toxicological Research FDA, Jefferson, Arkansas, USA
    Curr Protoc Toxicol . 2009
    ..This unit will describe the current methods for measuring azoreductase from Staphylococcus aureus, which has been suggested to serve as a model strain to study the azo dye degradation by human skin microflora...
  16. ncbi request reprint Toxicological significance of azo dye metabolism by human intestinal microbiota
    Jinhui Feng
    Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079 9502, USA
    Front Biosci (Elite Ed) 4:568-86. 2012
    ..This review highlights how azo dyes are metabolized by intestinal bacteria, mechanisms of azo reduction, and the potential contribution in the carcinogenesis/mutagenesis of the reduction of the azo dyes by intestinal microbiota...
  17. ncbi request reprint Recent advances in azo dye degrading enzyme research
    Huizhong Chen
    Division of Microbiology, National Center for Toxicological Research, U S FDA, 3900 NCTR Rd, Jefferson, AR 72079 9502, USA
    Curr Protein Pept Sci 7:101-11. 2006
    ..In this review, structures and carcinogenicity of azo colorants, protein structure, enzymatic function, and substrate specificity, as well as application of the azo dyes and azoreductases will be discussed...
  18. ncbi request reprint Microarray method to monitor 40 intestinal bacterial species in the study of azo dye reduction
    Rong Fu Wang
    Microbiology Division, National Center for Toxicological Research, FDA, Jefferson, AR 72079, USA
    Biosens Bioelectron 20:699-705. 2004
    ..Results from this study showed 26-30 species are present in the cultured fecal samples. The representative bacteria were then examined for the azo dye reduction activity...
  19. ncbi request reprint Crystal structure of an aerobic FMN-dependent azoreductase (AzoA) from Enterococcus faecalis
    Zhi Jie Liu
    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
    Arch Biochem Biophys 463:68-77. 2007
    ..A putative NADH binding site could be identified and a plausible mechanism for substrate reduction is proposed. Expression studies revealed azoA gene to be expressed constitutively in E. faecalis...
  20. ncbi request reprint Properties of a recombinant beta-glucosidase from polycentric anaerobic fungus Orpinomyces PC-2 and its application for cellulose hydrolysis
    Xin Liang Li
    Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, USDA ARS, 1815 N University Street, Peoria, IL 61604 3902, USA
    Appl Biochem Biotechnol 113:233-50. 2004
    ..6 mM. Beta-glucosidase significantly enhanced the conversion of cellulosic materials into glucose by Trichoderma reesei cellulase preparations, demonstrating its potential for use in biofuel and feedstock chemical production...
  21. ncbi request reprint CelF of Orpinomyces PC-2 has an intron and encodes a cellulase (CelF) containing a carbohydrate-binding module
    Huizhong Chen
    Center for Biological Resource Recovery, Department of Biochemistry and Molecular Biology, Life Science Building, The University of Georgia, Athens, GA 30602 7229
    Appl Biochem Biotechnol 105:775-85. 2003
    ..The gene has a 111-bp intron, located within the CBM-coding region. Some biochemical properties of the purified recombinant enzyme are described...
  22. ncbi request reprint A mannanase, ManA, of the polycentric anaerobic fungus Orpinomyces sp. strain PC-2 has carbohydrate binding and docking modules
    Eduardo A Ximenes
    Laboratorio De Enzimologia, Departmento de Biologia Celular, Universidade de Brasilia, Brazil
    Can J Microbiol 51:559-68. 2005
    ..coli bound preferably to insoluble lignocellulosic substrates, suggesting that it might play an important role in the complex enzyme system of the fungus for lignocellulose degradation...