Research Topics
Species | Huizhong ChenSummaryAffiliation: Food and Drug Administration Country: USA Publications
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Detail Information
Publications
Functional role of Trp-105 of Enterococcus faecalis azoreductase (AzoA) as resolved by structural and mutational analysisHuizhong Chen
Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079 9502, USA
Microbiology 154:2659-67. 2008..2, 30.6 and 8.2-fold, respectively). However, these mutated enzymes maintained K(m) values similar to the wild-type enzyme. This study provides an insight into the catalytic properties of AzoA in FMN stabilization and enzyme activity...
Identification and molecular characterization of a novel flavin-free NADPH preferred azoreductase encoded by azoB in Pigmentiphaga kullae K24Huizhong Chen
Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079 9502, USA
BMC Biochem 11:13. 2010..In this study, we report the identification of a novel flavin-free NADPH preferred azoreductase encoded by azoB in Pigmentiphaga kullae K24...- Decolorization of water and oil-soluble azo dyes by Lactobacillus acidophilus and Lactobacillus fermentumHuizhong Chen
Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079 9502, USA
J Ind Microbiol Biotechnol 36:1459-66. 2009..Aniline and o-toluidine (2-methylaniline), both potentially carcinogenic aromatic amines, were metabolites of Sudan III and IV, respectively... 
Isolation of extremely AT-rich genomic DNA and analysis of genes encoding carbohydrate-degrading enzymes from Orpinomyces sp. strain PC-2Huizhong Chen
Division of Microbiology, National Center for Toxicological Research, U S FDA, Jefferson, AR, 72079, USA
Curr Microbiol 53:396-400. 2006....- High level expression and characterization of the cyclophilin B gene from the anaerobic fungus Orpinomyces sp. strain PC-2Huizhong Chen
Division of Microbiology, National Center for Toxicological Research, U S FDA, 3900 NCTR Rd, Jefferson, AR 72079, USA
Protein Pept Lett 13:727-32. 2006..The overall architecture of the CypB molecule is very similar to that of human CypB... - Probing the NADH- and Methyl Red-binding site of a FMN-dependent azoreductase (AzoA) from Enterococcus faecalisJinhui Feng
Division of Microbiology, National Center for Toxicological Research, U S Food and Drug Administration, Jefferson, AR 72079, United States
Arch Biochem Biophys 520:99-107. 2012.... - Sudan azo dyes and Para Red degradation by prevalent bacteria of the human gastrointestinal tractHaiyan Xu
Division of Microbiology, National Center for Toxicological Research, U S Food and Drug Administration, Jefferson, AR 72079 9502, USA
Anaerobe 16:114-9. 2010..faecalis were isolated and identified by HPLC and LC/ESI-MS analyses and compared with authentic standards. Thus it appears that the ability to reduce Sudan dyes and Para Red except Sudan II is common among bacteria in the human colon... - Anaerobic metabolism of 1-amino-2-naphthol-based azo dyes (Sudan dyes) by human intestinal microfloraHaiyan Xu
Division of Microbiology, National Center for Toxicological Research, U S FDA, 3900 NCTR Rd, Jefferson, AR 72079 9502, USA
Appl Environ Microbiol 73:7759-62. 2007..These data indicate that human intestinal bacteria are able to reduce Sudan dyes to form potentially carcinogenic aromatic amines... - Effects of Orange II and Sudan III azo dyes and their metabolites on Staphylococcus aureusHongmiao Pan
Division of Microbiology, National Center for Toxicological Research, US FDA, 3900 NCTR Rd, Jefferson, AR 72079 9502, USA
J Ind Microbiol Biotechnol 38:1729-38. 2011..p-Phenylenediamine exhibited a synergistic effect with 1-amino-2-naphthol on cell growth inhibition. All of the dye metabolites had no significant effects on cell viability of the bacterium... - Molecular cloning, overexpression, purification, and characterization of an aerobic FMN-dependent azoreductase from Enterococcus faecalisHuizhong Chen
Division of Microbiology, National Center for Toxicological Research, US FDA, 3900 NCTR Rd, Jefferson, AR 72079 9502, USA
Protein Expr Purif 34:302-10. 2004..AzoA is the first aerobic azoreductase to be identified and characterized from human intestinal gram-positive bacteria... - Metabolism of azo dyes by human skin microbiotaRobin L Stingley
Office of Scientific Coordination, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079 9502, USA
J Med Microbiol 59:108-14. 2010..delphini, Staph. sciuri subsp. sciuri and Staphylococcus auricularis. The data presented in this report will be useful in the risk assessment process for evaluation of public exposure to products containing these dyes... - Evaluation of impact of exposure of Sudan azo dyes and their metabolites on human intestinal bacteriaHongmiao Pan
Division of Microbiology, National Center for Toxicological Research, U S Food and Drug Administration, Jefferson, AR 72079, USA
Anaerobe 18:445-53. 2012..The present results suggested that Sudan azo dyes and their metabolites potentially affect the human intestinal bacterial ecology by selectively inhibiting some bacterial species, which may have an adverse effect on human health... - Biochemical and molecular characterization of an azoreductase from Staphylococcus aureus, a tetrameric NADPH-dependent flavoproteinHuizhong Chen
Division of Microbiology, National Center for Toxicological Research, US FDA, 3900 NCTR Road, Jefferson, AR 72079 9502, USA
Microbiology 151:1433-41. 2005..4 microM min(-1) (mg protein)(-1). Azo1 was also able to metabolize Orange II, Amaranth, Ponceau BS and Ponceau S azo dyes. Azo1 represents the first azoreductase to be identified and characterized from human skin microflora... - Azoreductase in Staphylococcus aureusWen Zou
National Center for Toxicological Research FDA, Jefferson, Arkansas, USA
Curr Protoc Toxicol . 2009..This unit will describe the current methods for measuring azoreductase from Staphylococcus aureus, which has been suggested to serve as a model strain to study the azo dye degradation by human skin microflora... - Toxicological significance of azo dye metabolism by human intestinal microbiotaJinhui Feng
Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079 9502, USA
Front Biosci (Elite Ed) 4:568-86. 2012..This review highlights how azo dyes are metabolized by intestinal bacteria, mechanisms of azo reduction, and the potential contribution in the carcinogenesis/mutagenesis of the reduction of the azo dyes by intestinal microbiota... - Recent advances in azo dye degrading enzyme researchHuizhong Chen
Division of Microbiology, National Center for Toxicological Research, U S FDA, 3900 NCTR Rd, Jefferson, AR 72079 9502, USA
Curr Protein Pept Sci 7:101-11. 2006..In this review, structures and carcinogenicity of azo colorants, protein structure, enzymatic function, and substrate specificity, as well as application of the azo dyes and azoreductases will be discussed... - Microarray method to monitor 40 intestinal bacterial species in the study of azo dye reductionRong Fu Wang
Microbiology Division, National Center for Toxicological Research, FDA, Jefferson, AR 72079, USA
Biosens Bioelectron 20:699-705. 2004..Results from this study showed 26-30 species are present in the cultured fecal samples. The representative bacteria were then examined for the azo dye reduction activity... - Identification of the enzyme responsible for N-acetylation of norfloxacin by Microbacterium sp. Strain 4N2-2Dae Wi Kim
Divisions of Microbiology, U S Food and Drug Administration, Jefferson, Arkansas, USA
Appl Environ Microbiol 79:314-21. 2013..The results suggest that environmental bacteria whose enzymes modify fluoroquinolones may be able to survive in the presence of low fluoroquinolone concentrations... - Crystal structure of an aerobic FMN-dependent azoreductase (AzoA) from Enterococcus faecalisZhi Jie Liu
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
Arch Biochem Biophys 463:68-77. 2007..A putative NADH binding site could be identified and a plausible mechanism for substrate reduction is proposed. Expression studies revealed azoA gene to be expressed constitutively in E. faecalis... - Properties of a recombinant beta-glucosidase from polycentric anaerobic fungus Orpinomyces PC-2 and its application for cellulose hydrolysisXin Liang Li
Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, USDA ARS, 1815 N University Street, Peoria, IL 61604 3902, USA
Appl Biochem Biotechnol 113:233-50. 2004..6 mM. Beta-glucosidase significantly enhanced the conversion of cellulosic materials into glucose by Trichoderma reesei cellulase preparations, demonstrating its potential for use in biofuel and feedstock chemical production... - CelF of Orpinomyces PC-2 has an intron and encodes a cellulase (CelF) containing a carbohydrate-binding moduleHuizhong Chen
Center for Biological Resource Recovery, Department of Biochemistry and Molecular Biology, Life Science Building, The University of Georgia, Athens, GA 30602-7229
Appl Biochem Biotechnol 105:775-85. 2003..The gene has a 111-bp intron, located within the CBM-coding region. Some biochemical properties of the purified recombinant enzyme are described... - A mannanase, ManA, of the polycentric anaerobic fungus Orpinomyces sp. strain PC-2 has carbohydrate binding and docking modulesEduardo A Ximenes
Laboratorio De Enzimologia, Departmento de Biologia Celular, Universidade de Brasilia, Brazil
Can J Microbiol 51:559-68. 2005..coli bound preferably to insoluble lignocellulosic substrates, suggesting that it might play an important role in the complex enzyme system of the fungus for lignocellulose degradation...