Research Topics
| Abdu I AlayashSummaryAffiliation: Food and Drug Administration Country: USA Publications
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Detail Information
Publications
Setbacks in blood substitutes research and development: a biochemical perspectiveAbdu I Alayash
Division of Hematology, Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration PHS, 8800 Rockville Pike, Bethesda, MD 20892, USA
Clin Lab Med 30:381-9. 2010..It is hoped that this will aid in the development of a safe and effective second generation of HBOCs...- Haptoglobin: the hemoglobin detoxifier in plasmaAbdu I Alayash
Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA Electronic address
Trends Biotechnol 31:2-3. 2013..Potential therapeutic benefits of using Hp for inactivation and clearance of free Hb under a number of clinical settings are considered... 
First-generation blood substitutes: what have we learned? Biochemical and physiological perspectivesAbdu I Alayash
Center for Biologics Evaluation and Research, Food and Drug Administration, Laboratory of Biochemistry and Vascular Biology, Division of Hematology, National Institutes of Health Campus, Bethesda, MD 20892, USA
Expert Opin Biol Ther 7:665-75. 2007..Here the authors provide an overview of their research experiences, novel insights into the molecular basis of toxicities of these products and some lessons learned...- Effects of cross-linking and zero-link polymerization on oxygen transport and redox chemistry of bovine hemoglobinYiping Jia
Laboratory of Biochemistry and Vascular Biology LBVB, Division of Hematology, Center for Biologics Evaluation and Research CBER, Food and Drug Administration FDA, MD 20892, USA
Biochim Biophys Acta 1794:1234-42. 2009.... - Effects of endogenous ascorbate on oxidation, oxygenation, and toxicokinetics of cell-free modified hemoglobin after exchange transfusion in rat and guinea pigPaul W Buehler
Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
J Pharmacol Exp Ther 323:49-60. 2007..The present findings suggest the importance of plasma AA in maintaining the stability of acellular Hb susceptible to oxidation, and they may be relevant to humans, which display a similar plasma/tissue antioxidant status to guinea pig... - Induction of hypoxia inducible factor (HIF-1α) in rat kidneys by iron chelation with the hydroxypyridinone, CP94Jin Hyen Baek
Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
Biochim Biophys Acta 1809:262-8. 2011..In conclusion, we have identified the inhibition of iron-binding pocket of PHD as an underlying mechanism of HIF induction in vivo and in vitro by a bidentate hydroxypyridinone... - Structural basis of peroxide-mediated changes in human hemoglobin: a novel oxidative pathwayYiping Jia
Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
J Biol Chem 282:4894-907. 2007.... - Structural and functional characterization of glutaraldehyde-polymerized bovine hemoglobin and its isolated fractionsPaul W Buehler
Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research (CBER, Food and Drug Administration (FDA, Bethesda, Maryland 20892, USA
Anal Chem 77:3466-78. 2005..Structural modification imparted by glutaraldehyde resulted in nearly identical functional characteristics of PolyHbBv and its fractions with regard to oxygen equilibrium, ligand binding, and autoxidative kinetics... - Oxygen sensing in the circulation: "cross talk" between red blood cells and the vasculaturePaul W Buehler
Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Bethesda, MD 20892, USA
Antioxid Redox Signal 6:1000-10. 2004..We believe that there are important and yet unexplored mechanisms by which RBCs can directly or indirectly communicate via redox intermediates with extravascular sites as part of the global O(2) sensing mechanism... - Oxygen binding and oxidation reactions of human hemoglobin conjugated to carboxylate dextranYiping Jia
Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
Biochim Biophys Acta 1672:164-73. 2004..However, the reduction in the allosteric function of this protein and the lack of apparent quaternary T-->R transition may hinder its physiological role as an oxygen transporter... - Isolated Hb Providence β82Asn and β82Asp fractions are more stable than native HbA(0) under oxidative stress conditionsBindu Abraham
Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, US Food and Drug Administration, Bethesda, Maryland 20892, United States
Biochemistry 50:9752-66. 2011.... - Inactivation of prolyl hydroxylase domain (PHD) protein by epigallocatechin (EGCG) stabilizes hypoxia-inducible factor (HIF-1α) and induces hepcidin (Hamp) in rat kidneyDominador J Manalo
Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
Biochem Biophys Res Commun 416:421-6. 2011..These data demonstrate EGCG's therapeutic potential in modulating hepcidin expression in diseases associated with altered iron metabolism... - O-raffinose crosslinked hemoglobin lacks site-specific chemistry in the central cavity: structural and functional consequences of beta93Cys modificationRobert A Boykins
Laboratory of Biophysics, Division of Bacterial, Parasitic and Allergenic Products, Bethesda, Maryland 20892, USA
Proteins 59:840-55. 2005..Structural data presented here when taken together with the oxidative instability of O-R-PolyHbA0 may provide some basis for the reported toxicity of this oxygen carrier... - Effects of cell-free hemoglobin on hypoxia-inducible factor (HIF-1alpha) and heme oxygenase (HO-1) expressions in endothelial cells subjected to hypoxiaLi Hong Yeh
Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
Antioxid Redox Signal 6:944-53. 2004..DBBF-Hb modulates key cell-signaling pathways by competing with peroxides required for the deactivation of HIF-1alpha, which may modulate important physiological mediators... 
Familial secondary erythrocytosis due to increased oxygen affinity is caused by destabilization of the T state of hemoglobin Brigham (α₂β₂(Pro100Leu))Todd L Mollan
Laboratory of Biochemistry and Vascular Biology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA
Protein Sci 21:1444-55. 2012..These kinetic data help explain the high O₂ affinity characteristics of Hb Brigham and provide further evidence for the importance of the contribution of Pro100 to intersubunit contacts and stabilization of the T quaternary structure...- Heme binding to human alpha-1 proteinase inhibitorElena Karnaukhova
Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
Biochim Biophys Acta 1820:2020-9. 2012..While heme binding by hemopexin, albumin and α(1)-microglobulin has been extensively studied, the role of other plasma proteins remains largely unknown... - Acellular haemoglobin attenuates hypoxia-inducible factor-1alpha (HIF-1alpha) and its target genes in haemodiluted ratsDominador J Manalo
LBVB Laboratory of Biochemistry and Vascular Biology, Division of Hematology, CBER Center for Biologics Evaluation and Research, FDA Food and Drug Administration, NIH National Institutes of Health, Bethesda, MD 20892, USA
Biochem J 414:461-9. 2008.... - Cross-linking with O-raffinose lowers oxygen affinity and stabilizes haemoglobin in a non-cooperative T-state conformationYiping Jia
Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research (CBER, Food and Drug Administration (FDA, Bethesda, Maryland 20892, USA
Biochem J 384:367-75. 2004..Although the physiological ramifications of locking HbA(0) in the T conformation with the O-raffinose are still unknown, valuable insights into haemoglobin function are provided by these studies of O-R-polyHbA(0)... - Effects of (-)-epigallocatechin gallate on the redox reactions of human hemoglobinYiping Jia
Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, National Institutes of Health Campus, Bethesda, MD 20892, USA
Free Radic Biol Med 45:659-66. 2008..A balance between pro and antioxidant properties of EGCG should be taken into account if EGCG is used in combination therapy with redox active acellular Hbs... - A role for the myoglobin redox cycle in the induction of endothelial cell apoptosisFELICE D'AGNILLO
Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
Free Radic Biol Med 33:1153-64. 2002..These results suggest a role for the Mb redox cycle in the induction of endothelial cell apoptosis, which may be relevant in the pathophysiology of diseases characterized by the release of Mb from damaged muscle... - All hemoglobin-based oxygen carriers are not created equallyPaul W Buehler
Laboratory of Biochemistry and Vascular Biology LBVB, Division of Hematology, Center for Biologics Evaluation and Research CBER, Food and Drug Administration FDA, Maryland 20892, USA
Biochim Biophys Acta 1784:1378-81. 2008.... - Oxygen therapeutics: can we tame haemoglobin?Abdu I Alayash
Laboratory of Biochemistry, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, 8800 Rockville Pike, National Institutes of Health, Bethesda, Maryland 20892, USA
Nat Rev Drug Discov 3:152-9. 2004..Current protective strategies designed to produce safe Hb-based products are focused on controlling or suppressing the 'radical' nature of Hb while retaining its oxygen-carrying function... - Toxicities of hemoglobin solutions: in search of in-vitro and in-vivo model systemsPaul W Buehler
Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Bethesda, Maryland, USA
Transfusion 44:1516-30. 2004.... - Redox biology of blood revisited: the role of red blood cells in maintaining circulatory reductive capacityPaul W Buehler
Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA
Antioxid Redox Signal 7:1755-60. 2005..In the current short review, we have revisited the topic of redox biology of blood and focused on yet another emerging area of research, which deals with the reductive power of blood and the physiological Redox Signal... - Blood aging, safety, and transfusion: capturing the "radical" menacePaul W Buehler
Division of Hematology, Center for Biologics Evaluation and Research, U S Food and Drug Administration, Bethesda, Maryland 20892, USA
Antioxid Redox Signal 14:1713-28. 2011..Furthermore, some of the same naturally occurring protective mechanisms can potentially be employed to oxidatively inactivate this redox active protein and control its damaging side reactions when released outside of the RBC... - Structural stabilization in tetrameric or polymeric hemoglobin determines its interaction with endogenous antioxidant scavenger pathwaysPaul W Buehler
Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research CBER, U S Food and Drug Administration FDA, Rockville, Maryland, USA
Antioxid Redox Signal 10:1449-62. 2008..Based on these results, a rational and systematic approach to HBOC design may be used to optimize interaction with endogenous Hb clearance and detoxification pathways... - Chemical characterization of diaspirin cross-linked hemoglobin polymerized with poly(ethylene glycol)Paul W Buehler
Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research (CBER, Food and Drug Administration (FDA, Bethesda, Maryland 20892, USA
Anal Chem 78:4634-41. 2006..e., 64 kDa) has a direct influence on HBOC-mediated vasoactivity and that other protective strategies should be considered to control blood pressure imbalances... - α-Hemoglobin stabilizing protein (AHSP) markedly decreases the redox potential and reactivity of α-subunits of human HbA with hydrogen peroxideTodd L Mollan
Laboratory of Biochemistry and Vascular Biology, Division of Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20852, USA
J Biol Chem 288:4288-98. 2013..Hexacoordination in the AHSP·met-α complex markedly decreases the rate of the initial H(2)O(2) reaction with iron and thus provides α-subunits protection against damaging oxidative reactions... - Stopped-flow fluorescence method for the detection of heme degradation products in solutions of chemically modified hemoglobins and peroxideYiping Jia
Center for Biologics Evaluation and Research (CBER, Food and Drug Administration (FDA, Bethesda, Maryland 20892, USA
Anal Biochem 308:186-8. 2002 - The heme pocket geometry of Lucina pectinata hemoglobin II restricts nitric oxide and peroxide entry: model of ligand control for the design of a stable oxygen carrierWalleska De Jesus Bonilla
Department of Chemistry, University of Puerto Rico, Mayaguez Campus, P O Box 9019, Mayagüez 00681 9019, Puerto Rico
Biochemistry 46:10451-60. 2007..Engineering of second-generation Hb-based oxygen therapeutics that are resistant to NO/H2O2-driven oxidation may ultimately require further optimization of the heme pocket architecture to limit heme exposure to solvent... - Comparison of effects of two hemoglobin-based O(2) carriers on intestinal integrity and microvascular leakageAnn L Baldwin
Department of Physiology, College of Medicine, University of Arizona, Tucson 85724 5051, USA
Am J Physiol Heart Circ Physiol 283:H1292-301. 2002..These results indicate that intravascular PolyHbBv produces significantly less disruption of the intestinal exchange barrier than does DBBF-Hb, probably because the heme is not so easily oxidized... - Sodium selenite reduces hemoglobin-induced venular leakage in the rat mesenteryAnn L Baldwin
Department of Physiology, College of Medicine, University of Arizona, Tucson 85724 5051, USA
Am J Physiol Heart Circ Physiol 284:H81-91. 2003..In vitro, Na(2)SeO(3) reduced the oxidation rate of DBBF-Hb while in the presence of oxidants. These results suggest that Na(2)SeO(3) reduces DBBF-Hb-induced microvascular leakage partly by retarding the oxidation of its heme iron... - Differential effects of sodium selenite in reducing tissue damage caused by three hemoglobin-based oxygen carriersAnn L Baldwin
Department of Physiology, College of Medicine, University of Arizona, Tucson, Arizona 85724 5051, USA
J Appl Physiol 96:893-903. 2004.... - Site-specific cross-linking of human and bovine hemoglobins differentially alters oxygen binding and redox side reactions producing rhombic heme and heme degradationEnika Nagababu
Molecular Dynamics Section, National Institute on Aging/NIH, 5600 Nathan Shock Drive, Baltimore, MD 21224-6823, USA
Biochemistry 41:7407-15. 2002..These findings establish the importance of these secondary oxidative reactions over autoxidation in evaluating the toxicity of HBOCs... - CD163 is the macrophage scavenger receptor for native and chemically modified hemoglobins in the absence of haptoglobinDominik J Schaer
Medical Clinic B Research Unit, University Hospital, CH 8091 Zurich, Switzerland
Blood 107:373-80. 2006..These results identify CD163 as a scavenger receptor for native Hb and small-molecular-weight Hb-based blood substitutes after Hp depletion... - Ascorbate removes key precursors to oxidative damage by cell-free haemoglobin in vitro and in vivoJacqueline Dunne
Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK
Biochem J 399:513-24. 2006..g. haemolytic anaemias, subarachnoid haemorrhage, rhabdomyolysis... - Allosteric effects on oxidative and nitrosative reactions of cell-free hemoglobinsCelia Bonaventura
Nicholas School of the Environment and Earth Sciences, Duke University Marine Laboratory, Beaufort, North Carolina 28516, USA
IUBMB Life 59:498-505. 2007..These redox reactions can drive formation of SNO-Hb and other reactive species and are of significance for the use of cell-free Hbs in vivo... - Hemodilution with stroma-free [correction of stoma-free] hemoglobin at physiologically maintained viscosity delays the onset of vasoconstrictionGéraldine Rochon
Department of Hematology and Physiology, School of Pharmacy, University Henri Poincaré Nancy, France
Hypertension 43:1110-5. 2004.... - Gating the radical hemoglobin to macrophages: the anti-inflammatory role of CD163, a scavenger receptorDominik J Schaer
Medical Clinic B Research Unit, University of Zurich, Switzerland
Antioxid Redox Signal 9:991-9. 2007..Here, an overview and novel insights into the role of CD163 in Hb redox inactivation and clearance are provided... - Redox biology of bloodAbdu I Alayash
Antioxid Redox Signal 6:941-3. 2004