Research Topics
| S F Al KhaldiSummaryAffiliation: Food and Drug Administration Country: USA Publications
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Detail Information
Publications
DNA microarray technology used for studying foodborne pathogens and microbial habitats: minireviewSufian F Al-Khaldi
U S Food and Drug Administration, CFSAN, Division of Microbiological Studies, College Park, MD 20740 3855, USA
J AOAC Int 85:906-10. 2002..This minireview addresses the use of microarray technology in bacterial identification and gene expression in different microbial systems and in habitats containing mixed populations of bacteria...
Identification and characterization of Clostridium perfringens using single target DNA microarray chipSufian F Al-Khaldi
HFS 517, Division of Microbiological Studies, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740 3855, USA
Int J Food Microbiol 91:289-96. 2004..perfringens was detected by hybridization of amplified ssDNA probes to oligonucleotides on the chip representing one target sequence of each toxin gene. The DNA chip was able to identify eight strains of C. perfringens...
Genotyping of Clostridium perfringens toxins using multiple oligonucleotide microarray hybridizationS F Al-Khaldi
Division of Microbiological Studies, Center for Food Safety and Applied Nutrition, Food and Drug Administration, 5100 Paint Branch Parkway, College Park, MD 20740 3855, USA
Mol Cell Probes 18:359-67. 2004..Results of the study showed sensitivity and specificity of genotyping C. perfringens using multiple microarray-based assays...
Accelerating bacterial identification by infrared spectroscopy by employing microarray deposition of microorganismsSufian F Al-Khaldi
Division of Microbiological Studies, OPDFB, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland 20740 3855, USA
Foodborne Pathog Dis 1:172-7. 2004..Within 3 h, sufficient bacterial cells were obtained to allow accurate identification of the microorganism by infrared spectroscopy. This approach allows a "complete-in-a-single-day" analysis of a large array of samples...
Using PCR amplification to increase the confidence level of Salmonella typhimurium DNA microarray chip hybridizationShannon Courtney
HFS-517, Division of Microbiological Studies, Food and Drug Administration, Center for Food Safety and Applied Nutrition, 5100 Paint Branch Parkway, College Park, MD 20740-3855, USA
Mol Cell Probes 20:163-71. 2006..typhimurium was used as a standard to verify the confidence level of the DNA microarray chip. In conclusion, using PCR amplification to increase the confidence level of the microarray hybridization data was successful...
Application of a disposable transparent filtration membrane to the infrared spectroscopic discrimination among bacterial speciesM M Mossoba
Division of General Scientific Support DGSS, OSAS, Food and Drug Administration FDA, Center for Food Safety and Applied Nutrition CFSAN, College Park, MD, USA
J Microbiol Methods 55:311-4. 2003..Observed cellular infrared spectra were unique and were used to discriminate among the different species. A dataset for the 10 bacterial species investigated was successfully used to correctly identify unknowns included in the dataset...
Gene and bacterial identification using high-throughput technologies: genomics, proteomics, and phonemicsSufian F Al-Khaldi
Division of Microbiological Studies, OPDFB, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland 20740-3855, USA
Nutrition 20:32-8. 2004
Multipathogen oligonucleotide microarray for environmental and biodefense applicationsNikolay Sergeev
FDA Center for Food Safety and Applied Nutrition, College Park, MD, USA
Biosens Bioelectron 20:684-98. 2004..The results presented demonstrate the potential of oligonucleotide microarrays for detection of environmental and biodefense relevant microbial pathogens...
Use of fatty acid profiles to identify food-borne bacterial pathogens and aerobic endospore-forming bacilliPaul Whittaker
Center for Food Safety and Applied Nutrition, Food and Drug Administration, 5100 Paint Branch Parkway, College Park, Maryland 20740 3835, USA
J Agric Food Chem 53:3735-42. 2005..anthracis and B. cereus spores but not in the vegetative cells. In summary, analysis of FAMEs from bacteria and spores can provide a sensitive procedure for the identification of food-borne pathogens...
Application of a novel hydrophilic infrared-transparent membrane to the differentiation between microcolonies of Enterobacter sakazakii and Klebsiella pneumoniaeM M Mossoba
Division of Analytical Chemistry, Center for Food Safety and Applied Nutrition, U S Food and Drug Administration, 5100 Paint Branch Parkway, College Park, Maryland 20740 3835, USA
J Food Prot 70:1241-5. 2007..Multivariate analysis of the observed IR spectra for microcolonies allowed the rapid differentiation between E. sakazakii and K. pneumoniae...
Fourier transform infrared bacteria identification with the use of a focal-plane-array detector and microarray printingJonah Kirkwood
McGill IR Group, Dept. of Food Science and Agricultural Chemistry, McGill University, Montreal, Quebec, Canada
Appl Spectrosc 58:1364-8. 2004
Cloning of the O-acetylserine lyase gene from the ruminal bacterium Selenomonas ruminantium HD4Jeff D Evans
Department of Animal and Dairy Science, 312 Animal and Dairy Science Complex, University of Georgia, Athens, GA 30602-2771, USA
Curr Microbiol 44:161-6. 2002..ruminantium HD4. To our knowledge, this is the first report characterizing genes involved in sulfur metabolism from the genus Selenomonas...
