Daniel L Kaplan
Affiliation: Florida State University
Pérez Arnaiz P, Bruck I, Kaplan D. Mcm10 coordinates the timely assembly and activation of the replication fork helicase. Nucleic Acids Res. 2016;44:315-29 pubmed publisher
..Mcm10 is first involved in the recruitment of Cdc45 to the Mcm2-7 complex. After Cdc45-Mcm2-7 complex assembly, Mcm10 promotes origin melting by stimulating DDK phosphorylation of Mcm2, which thereby leads to GINS attachment to Mcm2-7. ..
Dhingra N, Bruck I, Smith S, Ning B, Kaplan D. Dpb11 protein helps control assembly of the Cdc45Â·Mcm2-7Â·GINS replication fork helicase. J Biol Chem. 2015;290:7586-601 pubmed publisher
..Finally, we propose that Dpb11 functions with Sld2 and Sld3 to help control the assembly of the replication fork helicase. ..
Pérez Arnaiz P, Kaplan D. An Mcm10 Mutant Defective in ssDNA Binding Shows Defects in DNA Replication Initiation. J Mol Biol. 2016;428:4608-4625 pubmed publisher
..These data suggest that DNA binding by Mcm10 is essential for the initiation of DNA replication. ..
Martinez M, Wacker A, Bruck I, Kaplan D. Eukaryotic Replicative Helicase Subunit Interaction with DNA and Its Role in DNA Replication. Genes (Basel). 2017;8: pubmed publisher
Bruck I, Kaplan D. Conserved mechanism for coordinating replication fork helicase assembly with phosphorylation of the helicase. Proc Natl Acad Sci U S A. 2015;112:11223-8 pubmed publisher
..These data suggest a conserved mechanism for replication initiation: Sld3/Treslin coordinates Cdc45 recruitment to Mcm2-7 with DDK phosphorylation of Mcm2 during S phase. ..
Bruck I, Dhingra N, Kaplan D. A Positive Amplification Mechanism Involving a Kinase and Replication Initiation Factor Helps Assemble the Replication Fork Helicase. J Biol Chem. 2017;292:3062-3073 pubmed publisher
..We conclude that Dpb11 functions with DDK and Mcm4 in a positive amplification mechanism to trigger the assembly of the replication fork helicase. ..
Martinez M, Jones J, Bruck I, Kaplan D. Origin DNA Melting-An Essential Process with Divergent Mechanisms. Genes (Basel). 2017;8: pubmed publisher
..We propose that although the process of origin melting is essential for the various domains of life, the mechanism for origin melting may be quite different among the different DNA replication initiation systems. ..