Research Topics
| Bruce BudowleSummaryAffiliation: Federal Bureau of Investigation Country: USA Publications
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Detail Information
Publications
Forensic aspects of mass disasters: strategic considerations for DNA-based human identificationBruce Budowle
Federal Bureau of Investigation Laboratory, 2501 Investigation Parkway, Quantico, VA 22135, USA
Leg Med (Tokyo) 7:230-43. 2005..Therefore, developing a missing persons DNA identification program would also provide the basis for a mass disaster human remains DNA identification program...
Bruce Budowle profile. FBI's top scientist takes the lead in forensic biologyMartin Enserink
Science 300:41-3. 2003
Toward a system of microbial forensics: from sample collection to interpretation of evidenceBruce Budowle
Federal Bureau of Investigation, Laboratory Division, 2501 Investigation Pkwy, Quantico, VA 22135, USA
Appl Environ Microbiol 71:2209-13. 2005
Partial matches in heterogeneous offender databases do not call into question the validity of random match probability calculationsBruce Budowle
FBI Laboratory, 2501 Investigation Parkway, Quantico, VA 22135, USA
Int J Legal Med 123:59-63. 2009..Defined population data, which are sufficiently abundant, have already demonstrated the validity of the basic assumptions of DNA forensic statistical assumptions...
Null allele sequence structure at the DYS448 locus and implications for profile interpretationBruce Budowle
Laboratory Division, FBI, Quantico, VA, 22135, USA
Int J Legal Med 122:421-7. 2008..The presence of null alleles is not a real concern for interpretation of Y STR loci evidence; current methods for interpreting Y STR profiles easily accommodate such phenomena...
The Microbial Rosetta Stone Database: a compilation of global and emerging infectious microorganisms and bioterrorist threat agentsDavid J Ecker
Ibis Therapeutics, 1891 Rutherford Rd, Carlsbad, CA 92008, USA
BMC Microbiol 5:19. 2005..This ambiguity can be a significant block to effective communication among the diverse communities that must deal with epidemics or bioterrorist attacks...
Genetic analysis and attribution of microbial forensics evidenceBruce Budowle
FBI Laboratory, Quantico, Virginia 22135, USA
Crit Rev Microbiol 31:233-54. 2005..Nucleic acid based typing technologies include: PCR, real-time PCR, MLST, MLVA, whole genome sequencing, and microarrays...
HIV legal precedent useful for microbial forensicsBruce Budowle
FBI Laboratory, 2501 Investigation Parkway, Quantico, VA 22135, USA
Croat Med J 46:514-21. 2005..The scientific evaluation involves epidemiology, molecular biology, phylogenetics, and legal deliberations...
Recommendations for animal DNA forensic and identity testingBruce Budowle
FBI Laboratory, 2501 Investigation Parkway, Quantico, VA 22135, USA
Int J Legal Med 119:295-302. 2005..These should provide a basis for professional societies and/or working groups to establish more formalized recommendations...
Microbial forensics: the next forensic challengeBruce Budowle
Laboratory Division, FBI, Quantico, VA 22135, USA
Int J Legal Med 119:317-30. 2005..Lastly, partnerships with other laboratories will be requisite, because many of the necessary capabilities for analysis will not reside in the traditional forensic laboratory...
SNP typing strategiesBruce Budowle
FBI Laboratory, Quantico, VA 22135, USA
Forensic Sci Int 146:S139-42. 2004..They are SNaP shot, SNPstream Ultra High Throughput System, and electrospray ionization mass spectrometry. The latter approach offers certain advantages for SNP analyses, particularly so for mtDNA typing...
Genetics and attribution issues that confront the microbial forensics fieldBruce Budowle
FBI Laboratory, 2501 Investigation Parkway, Quantico, VA 22135, USA
Forensic Sci Int 146:S185-8. 2004..Differences include: database size and composition, statistical interpretation methods, and confidence/uncertainty in the outcome of an interpretation...
Twelve short tandem repeat loci Y chromosome haplotypes: genetic analysis on populations residing in North AmericaBruce Budowle
Federal Bureau of 2501 Investigation Parkway, Investigation, Quantico, VA 22135, USA
Forensic Sci Int 150:1-15. 2005..e., Caucasian, African American, Hispanic, and Asian). These analyses support use of the haplotype population data for estimating Y-STR profile frequencies for populations residing in North America...
Role of law enforcement response and microbial forensics in investigation of bioterrorismBruce Budowle
Scientific Analysis Section, Federal Bureau of Investigation, Quantico, VA, USA
Croat Med J 48:437-49. 2007..The first is necessary for rapid response when a threat is imminent or an attack has recently occurred. The latter two apply to implementation of routinely used procedures...
Forensically relevant SNP classesBruce Budowle
FBI Laboratory, Quantico, VA, USA
Biotechniques 44:603-8, 610. 2008..In addition to discussing the applications of these different types of SNPs, this article provides some discussion on privacy issues so that society and policymakers can be more informed...
The effects of Asian population substructure on Y STR forensic analysesBruce Budowle
Federal Bureau of Investigation, Quantico, VA 22135, USA
Leg Med (Tokyo) 11:64-9. 2009..However haplotypes with fewer markers may require F(ST) corrections when calculating the rarity of the profile...
Public health. Building microbial forensics as a response to bioterrorismBruce Budowle
Federal Bureau of Investigation, Laboratory Division, Quantico, VA 22135, USA
Science 301:1852-3. 2003....
Quality sample collection, handling, and preservation for an effective microbial forensics programBruce Budowle
Laboratory Division, Federal Bureau of Investigation, Quantico, Virginia 22135, USA
Appl Environ Microbiol 72:6431-8. 2006
Mixture interpretation: defining the relevant features for guidelines for the assessment of mixed DNA profiles in forensic caseworkBruce Budowle
FBI Laboratory, 2501 Investigation Parkway, Quantico, VA 22134, USA
J Forensic Sci 54:810-21. 2009..The intent of this paper is to promote more thought, provide assistance on many aspects for consideration, and to support that more formalized mixture interpretation guidelines are developed...
Population studies on three Native Alaska population groups using STR lociBruce Budowle
FBI Laboratory, 935 Pennsylvania Avenue NW, Washington, DC 20535, USA
Forensic Sci Int 129:51-7. 2002..The F(ST) estimate for Athabaskans, Apaches and Navajos is 0.0180 and for Inupiats and Yupiks is 0.0167. The allele frequency data can be used for estimating DNA profile frequencies for Native Americans residing in Alaska...
Evaluation of circular DNA substrates for whole genome amplification prior to forensic analysisCourtney M Tate
Counterterrorism and Forensic Science Research Unit, Laboratory Division, Federal Bureau of Investigation, 2501 Investigation Parkway, Quantico, VA 22135, USA
Forensic Sci Int Genet 6:185-90. 2012..Further research and development is warranted prior to use of WGA in forensic casework...
Recommendations for consistent treatment of length variants in the human mitochondrial DNA control regionMark R Wilson
Counterterrorism and Forensic Science Research Unit, FBI Academy, Quantico, VA 22135, USA
Forensic Sci Int 129:35-42. 2002..Consistency in alignment and nomenclature avoids inadvertently describing two sequences as different when in fact they are the same...
An improved method for post-PCR purification for mtDNA sequence analysisKerri A Dugan
Laboratory Division, Federal Bureau of Investigation, Quantico, VA 22135, USA
J Forensic Sci 47:811-8. 2002..When the yield of purified amplicons, quality of the sequence profile, and ease of assay were evaluated, the use of ExoSAP-IT reagent for post-amplification purification was chosen to replace the filtration method...
Forensics and mitochondrial DNA: applications, debates, and foundationsBruce Budowle
Laboratory Division, FBI, Washington, DC 20535, USA
Annu Rev Genomics Hum Genet 4:119-41. 2003..We describe the approaches, the impact some issues may have on interpretation of mtDNA analyses, and some issues raised in the courtroom...
Quantification of human mitochondrial DNA using synthesized DNA standardsMark F Kavlick
Counterterrorism and Forensic Science Research Unit, Laboratory Division, Federal Bureau of Investigation, 2501 Investigation Parkway, Quantico, VA 22135, USA
J Forensic Sci 56:1457-63. 2011..The assay described is rapid, reliable, and robust...
Addressing the use of phylogenetics for identification of sequences in error in the SWGDAM mitochondrial DNA databaseBruce Budowle
FBI Laboratory, 2501 Investigation Parkway, Quantico, VA 22135, USA
J Forensic Sci 49:1256-61. 2004..Community wide access and review has enabled identification of errors in the SWGDAM data set and will continue to do so. The result of public accessibility is that the quality of the SWGDAM forensic dataset is always improving...
HVI and HVII mitochondrial DNA data in Apaches and NavajosBruce Budowle
Laboratory Division, FBI, 935 Pennsylvania Avenue, NW, Washington, DC 20535, USA
Int J Legal Med 116:212-5. 2002..0 in the Navajo sample and 7.7 in the Apache sample. The data demonstrate that mtDNA sequencing can be informative in forensic cases where Native American population data are used...
Evaluation of a multicapillary electrophoresis instrument for mitochondrial DNA typingJohn E B Stewart
National Missing Person DNA Database, DNA Analysis Unit II, Scientific Analysis Section, FBI Laboratory, FBI Academy, Quantico, VA 22135, USA
J Forensic Sci 48:571-80. 2003..The data support that valid and reliable results can be obtained using either capillary electrophoresis instrument, and the quality of sequencing results are comparable to or better than those obtained from the ABI 377 instrument...
Comparison of mitotyper rules and phylogenetic-based mtDNA nomenclature systemsDeborah Polanskey
FBI Laboratory, Quantico, VA 22135, USA
J Forensic Sci 55:1184-9. 2010..Using a rule-based approach, commonly occurring as well as private variants are subjected to the same rules for naming, which is particularly advantageous when typing partial sequence data...
Critique of interpretation of high levels of heteroplasmy in the human mitochondrial DNA hypervariable region I from hairBruce Budowle
Laboratory Division, Federal Bureau of Investigation, Washington, DC 20535, USA
Forensic Sci Int 126:30-3. 2002..The results observed in the heteroplasmy in hair study do not apply to other methods of mtDNA analysis and cannot be used to question the reliability of the current forensic mtDNA practices...
A perspective on errors, bias, and interpretation in the forensic sciences and direction for continuing advancementBruce Budowle
FBI Laboratory, 2501 Investigation Parkway, Quantico, VA 22135, USA
J Forensic Sci 54:798-809. 2009..The list is a starting point for discussion that could foster further thought and input in developing an overarching strategic plan for enhancing the forensic sciences...
Guatemala mestizo population data on 15 STR loci (Identifiler Kit)Esther Martinez-Espin
Laboratory of Genetic Identification, Department de Medicina Legal, Facultad de Medicina, Universidad de Granada, E-18012 Granada, Spain
J Forensic Sci 51:1216-8. 2006
Population data for four population groups from the United States for the eleven Y-chromosome STR loci recommended by SWGDAMJaiprakash G Shewale
ReliaGene Technologies Inc, 5525 Mounes St, Suite 101, New Orleans, LA 70123, USA
J Forensic Sci 51:700-2. 2006
STR population diversity in nine ethnic populations living in DubaiFarida Alshamali
Dubai Police Forensic Administration, Dubai, UAE
Forensic Sci Int 152:267-79. 2005
Population data on eight short tandem repeat loci in the Barbadian populationRachel-Anne C Henry
Forensic Sciences Centre, Culloden Road, St. Michael, Barbados
J Forensic Sci 51:440-1. 2006
Identification of a novel polymorphism in the X-chromosome region homologous to the DYS456 locusChien Wei Chang
Applied Biosystems, Foster City, CA 94404, USA
J Forensic Sci 51:344-8. 2006..Full profiles in a mixture of male:female at 1:4000 were obtained using the current configuration of the AmpFlSTR Yfiler kit even in the presence of female DNA containing the G variant...
Identification of a D8S1179 primer binding site mutation and the validation of a primer designed to recover null allelesCraig Leibelt
Applied Biosystems, 850 Lincoln Centre Drive, M S 404 3, Foster City, CA 94404, USA
Forensic Sci Int 133:220-7. 2003..No deleterious effects or non-specific peaks were observed in validation experiments evaluating primer concentration, Mg2+ concentration, annealing temperature and population samples...
Sequence polymorphism of the mitochondrial DNA hypervariable regions I and II in 205 Singapore MalaysHang Yee Wong
DNA Profiling Laboratory, Centre for Forensic Science, Health Sciences Authority, 11 Outram Road, Singapore 169078, Singapore
Leg Med (Tokyo) 9:33-7. 2007..Haplotype diversity and random match probability were estimated to be 0.9961% and 0.87%, respectively...
Minnesota population data on 15 STR loci using the Identifiler kitAnn Marie Gross
Minnesota BCA Forensic Science Laboratory, St. Paul, MN 55106, USA
J Forensic Sci 51:1410-3. 2006
Characterization of human control region sequences for Spanish individuals in a forensic mtDNA data setJ Carlos Alvarez
Department of Legal Medicine, University of Granada, Madrid, Granada, Spain
Leg Med (Tokyo) 9:293-304. 2007..The data were compared with and found to be similar to other published data sets. There were 109 SNPs observed in the data set, including 10 positions not previously reported. The most variable sites are consistent with other studies...
Evaluation of variation in control region sequences for Hispanic individuals in the SWGDAM mtDNA data setMarc W Allard
Department of Biological Sciences, George Washington University, Washington, DC 20052, USA
J Forensic Sci 51:566-73. 2006..The descriptive analyses revealed that the SWGDAM Hispanic data set is similar to published Native American and Hispanic data sets...
Typing of the locus DYS19 from DNA derived from fingernail clippings using PCR Concert rapid purification systemDavid A Gangitano
DNA Section, Chemical Laboratory Division, Federal Police, Argentina
J Forensic Sci 47:175-7. 2002..In this paper, an alternative DNA extraction procedure was employed, and the application of an STR locus residing on the Y chromosome DYS19 was typed to allow for genetic characterization of the perpetrator in such cases...
Characterization of the Caucasian haplogroups present in the SWGDAM forensic mtDNA dataset for 1771 human control region sequences. Scientific Working Group on DNA Analysis MethodsMarc W Allard
Department of Biological Sciences, George Washington University, Washington DC, USA
J Forensic Sci 47:1215-23. 2002..To evaluate the dataset for forensic utility, the haplogroup identifications and frequencies were compared with those reported from other published studies...
A novel SNaPshot assay to detect the mdx mutationSarah A Budowle
Department of Human Nutrition, Food and Exercise, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA
Muscle Nerve 37:731-5. 2008..e., SNaPshot) is described. The SNaPshot assay has been optimized to identify both wild-type and mutant alleles, providing a robust, potentially automatable assay for high-throughput analysis...
Development and validation of a multiplexed Y-chromosome STR genotyping system, Y-PLEX 6, for forensic caseworkSudhir K Sinha
ReliaGene Technologies, Inc 5525 Mounes St Suite 101, New Orleans, LA 70123, USA
J Forensic Sci 48:93-103. 2003..The data indicate that results obtained using the Y-PLEX 6 kit are robust, sensitive, and reliable and can be used in human forensic and male lineage identification cases...
Population data for seven Y-chromosome STR loci from three different population groups residing in ConnecticutHeather Miller Coyle
State of Connecticut Department of Public Safety, Division of Scientific Services, Forensic Science Laboratory, 278 Colony Street, Meriden, CT 06451, USA
J Forensic Sci 48:435-7. 2003
Y-chromosome STR haplotypes in three different population groups from Ecuador (South America)Fabricio González-Andrade
Molecular Genetics Laboratory, Metropolitan Hospital, Quito, Ecuador
J Forensic Sci 53:512-4. 2008..POPULATIONS: Over 102 unrelated Mestizos, 102 Native Amerindian (Kichwas), and 102 African Americans who represent the three largest communities of the country Ecuador (South America)...
Haplotype distribution of nine Y-chromosome STR-loci in two northern Spanish populations (Asturias and Aragon)Begoña Martínez-Jarreta
Department of Forensic Medicine, Faculty of Medicine, University of Zaragoza, Zaragoza, Spain
J Forensic Sci 48:204-5. 2003
Population data for the D5S818, D 13S317, D7S820 and D16S539 STR loci in a Romanian population sampleCatalin Marian
Biochemistry Department, Victor Babes University of Medicine and Pharmacy from Timisoara, Romania
J Forensic Sci 50:1512. 2005
Allele distribution of 15 STR loci used for human identity purposes in the Greek Cypriot population of the island of CyprusMarios A Cariolou
Laboratory of Forensic Genetics and Molecular Genetics Department B, Cyprus Institute of Neurology and Genetics, Airport Av No 6, 2370 Nicosia, Republic of Cyprus
Forensic Sci Int 164:75-8. 2006....
Epidemiology. DNA identifications after the 9/11 World Trade Center attackLeslie G Biesecker
Science 310:1122-3. 2005....
Mexican population data on fifteen STR loci (Identifiler kit) in a Chihuahua (North Central Mexico) sampleLuis J Martinez-Gonzalez
Laboratory of Genetic Identification Dept de Medicina Legal, Facultad de Medicina, Universidad de Granada, E 18012 Granada, Spain
J Forensic Sci 50:236-8. 2005
The application of an automated allele concordance analysis system (CompareCalls) to ensure the accuracy of single-source STR DNA profilesJohn H Ryan
Myriad Genetic Laboratories, 320 Wakara Way, Salt Lake City, Utah 84108, USA
J Forensic Sci 49:492-9. 2004..Implementation of an automated allele concordance analysis system will yield high-quality data for CODIS and free the human DNA data analyst to perform other critical duties within the laboratory...
Autosomic STR loci (HUMTPOX, HUMTH01, HUMVWA, D18S535, D1S1656 and D12S391) in San Salvador (El Salvador, Central America)Begoña Martínez-Jarreta
Department of Legal Medicine, University of Zaragoza, Faculty of Medicine, C Domingo Miral sn, 50009 Zaragoza, Spain
J Forensic Sci 49:633-4. 2004
FBI mtDNA database: a cogent perspectiveBruce Budowle
Science 307:845-7. 2005
STR data for the 13 CODIS loci in Singapore MalaysH C Ang
DNA Profiling Laboratory, Centre for Forensic Science, Health Sciences Authority, 11 Outram Road, Singapore 169078, Singapore
Forensic Sci Int 148:243-5. 2005....
Characterization of human control region sequences of the African American SWGDAM forensic mtDNA data setMarc W Allard
Department of Biological Sciences, George Washington University, Washington, DC 20052, USA
Forensic Sci Int 148:169-79. 2005..The genetic characterization of these relevant data sets is fully consistent with other published mtDNA genetic variation. The sequence diversity observed in this data set makes it a valuable tool for forensic applications...
Control region sequences for East Asian individuals in the Scientific Working Group on DNA Analysis Methods forensic mtDNA data setMarc W Allard
Department of Biological Sciences, George Washington University, Washington, DC 20052, USA
Leg Med (Tokyo) 6:11-24. 2004..European Caucasian, and African haplogroups were rarely observed within the East Asian data sets. The various analyses revealed that the data set was similar to published East Asian data sets such as those from Han Chinese...
Utility of the Y-STR typing systems Y-PLEX 6 and Y-PLEX 5 in forensic casework and 11 Y-STR haplotype database for three major population groups in the United StatesSudhir K Sinha
ReliaGene Technologies, Inc, New Orleans, LA 70123, USA
J Forensic Sci 49:691-700. 2004..Linkage analysis reveals that the frequencies from forensically important autosomal loci can be multiplied with the Y-STR haplotype frequency. The results from Y-PLEX systems have been accepted in courts in the United States...
Validation of a male-specific, 12-locus fluorescent short tandem repeat (STR) multiplexBenjamin E Krenke
Promega Corporation, Genetic Analysis Research and Development, Madison, WI 53716, USA
Forensic Sci Int 148:1-14. 2005..In general, the PowerPlex Y System was shown to have the sensitivity, specificity and reliability required for forensic DNA analysis...
Allele frequencies for markers CSF1PO, TPOX, TH01, F13A01, FESFPS, vWA, D16S539, D7S820, D13S317 in the general population of NicaraguaRafael E Flores-Obando
, Universidad Centroamericana, Apdo. 69, Managua, Nicaragua
J Forensic Sci 49:416-7. 2004..POPULATION: General Hispanic-admixed individuals from Nicaragua...
Brazilian Caucasian population data for 15 STR loci (PowerPlex 16 kit)Mauro S Figueiredo
Fleury Medical Diagnostic Center, Molecular Biology Section, Sao Paulo, Brazil
J Forensic Sci 49:167-9. 2004
Population data on 15 STR loci (PowerPlex 16 kit) in a Costa Rica (Central America) sample populationErna Melendez
OIJ-Laboratorio de Ciencias Forenses, , Heredia, Costa Rica
J Forensic Sci 49:170-2. 2004
Characterization of 17 Y-STR loci in a population from El Salvador (San Salvador, Central America) and their potential for DNA profilingBegoña Martínez-Jarreta
Department of Legal Medicine, University of Zaragoza, Faculty of Medicine, C Domingo Miral s n, 50 009 Zaragoza, Spain
J Forensic Sci 50:1243-6. 2005
Validation of male-specific, 12-locus fluorescent short tandem repeat (STR) multiplexBenjamin E Krenke
Promega Corporation, Genetic Analysis Research and Development, Madison, WI 53716, USA
Forensic Sci Int 151:111-24. 2005..In general, the PowerPlex Y System was shown to have the sensitivity, specificity and reliability required for forensic DNA analysis...
Genetic data for the 13 CODIS STR loci in Singapore IndiansS E S Lim
DNA Profiling Laboratory, Centre for Forensic Science, Health Sciences Authority, 11 Outram Road, Singapore 169078, Singapore
Forensic Sci Int 148:65-7. 2005..Allele frequencies for the 13 CODIS short tandem repeat (STR) loci included in the AmpFISTR Profiler Plus and AmpFISTR Cofiler kits (Applied Biosystems, Foster City, USA) were determined in a sample of 174 unrelated Indians in Singapore...
Base composition analysis of human mitochondrial DNA using electrospray ionization mass spectrometry: a novel tool for the identification and differentiation of humansThomas A Hall
Ibis Therapeutics, A Division of Isis Pharmaceuticals, 1891 Rutherford Road, Carlsbad, CA 92008, USA
Anal Biochem 344:53-69. 2005..This assay can be expanded to assess genetic variation in the coding region of the mtDNA genome and can be automated to facilitate analysis of a large number of samples such as those encountered after a mass disaster...
Genetic data for the 13 CODIS STR loci in Singapore ChineseC K C Syn
DNA Profiling Laboratory, Centre for Forensic Science, Health Sciences Authority, 11 Outram Road, Singapore 169078
Forensic Sci Int 152:285-8. 2005..6 x10(-15) and the overall probability of excluding paternity is 0.9999899. The results demonstrate that the loci are useful for forensic human identification and parentage testing for the Chinese population in Singapore...
Development and validation of the Y-PLEX 5, a Y-chromosome STR genotyping system, for forensic caseworkSudhir K Sinha
ReliaGene Technologies, Inc, 5525 Mounes St, Suite 101, New Orleans, LA 70123, USA
J Forensic Sci 48:985-1000. 2003..Y-STR allele and haplotype frequencies in two populations were generated. The data indicate that the Y-PLEX 5 genotyping system is sensitive and reliable, and can be used in human forensic and male lineage identification cases...
Population study of 11 Y-chromosomal STR loci in Singapore ChineseJune Sheau Wei Tang
DNA Profiling Laboratory, Centre for Forensic Science, Health Sciences Authority, 11 Outram Road, 169078 Singapore, Singapore
Forensic Sci Int 158:65-71. 2006..67%) were unique. The most common haplotype was observed in five (2.35%) individuals. The overall haplotype diversity for the 11 Y-STR loci was 99.81%, and the discrimination capacity was 86.79%...
An assessment of whether SNPs will replace STRs in national DNA databases--joint considerations of the DNA working group of the European Network of Forensic Science Institutes (ENFSI) and the Scientific Working Group on DNA Analysis Methods (SWGDAM)Peter Gill
Sci Justice 44:51-3. 2004
Getting the science and the ethics right in forensic geneticsMark Shriver
Nat Genet 37:449-50; author reply 450-1. 2005
Y-chromosome STR system, Y-PLEX 12, for forensic casework: development and validationJaiprakash G Shewale
ReliaGene Technologies, Inc. 5525 Mounes St. Suite 101, New Orleans, LA 70123, USA
J Forensic Sci 49:1278-90. 2004..Y-PLEX 12 is a sensitive, valid, reliable, and robust multiplex system for forensic analysis, and it can be used in human forensic and male lineage identification cases...
