Keith D Wilkinson

Summary

Affiliation: Emory University
Country: USA

Publications

  1. pmc The discovery of ubiquitin-dependent proteolysis
    Keith D Wilkinson
    Department of Biochemistry, Emory University School of Medicine, 4017 Rollins Research Building, 1510 Clifton Road, Atlanta, GA 30322, USA
    Proc Natl Acad Sci U S A 102:15280-2. 2005
  2. pmc The ubiquitin signal: assembly, recognition and termination. Symposium on ubiquitin and signaling
    Keith D Wilkinson
    Department of Biochemistry, 4017 Rollins Research Building, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    EMBO Rep 6:815-20. 2005
  3. ncbi request reprint Ubiquitin: a Nobel protein
    Keith D Wilkinson
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Cell 119:741-5. 2004
  4. pmc Recognition of polyubiquitin isoforms by the multiple ubiquitin binding modules of isopeptidase T
    Francisca E Reyes-Turcu
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 283:19581-92. 2008
  5. pmc Prion induction by the short-lived, stress-induced protein Lsb2 is regulated by ubiquitination and association with the actin cytoskeleton
    Tatiana A Chernova
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Mol Cell 43:242-52. 2011
  6. pmc Doa1 is a Cdc48 adapter that possesses a novel ubiquitin binding domain
    James E Mullally
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Mol Cell Biol 26:822-30. 2006
  7. ncbi request reprint Effects of ubiquitin system alterations on the formation and loss of a yeast prion
    Kim D Allen
    School of Biology and Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332 0230, USA
    J Biol Chem 282:3004-13. 2007
  8. ncbi request reprint Modulating huntingtin half-life alters polyglutamine-dependent aggregate formation and cell toxicity
    Michael D Kaytor
    Department of Human Genetics, Emory University School of Medicine, Atlanta, Georgia, USA
    J Neurochem 89:962-73. 2004
  9. pmc Hsp70 chaperones as modulators of prion life cycle: novel effects of Ssa and Ssb on the Saccharomyces cerevisiae prion [PSI+]
    Kim D Allen
    School of Biology and Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, 30332 0363, USA
    Genetics 169:1227-42. 2005
  10. ncbi request reprint Ubiquitin-proteasome degradation of KLF5 transcription factor in cancer and untransformed epithelial cells
    Ceshi Chen
    Department of Oncology and Hematology, Winship Cancer Institute, Emory University School of Medicine, 1365 C Clifton Road, Atlanta, GA, USA
    Oncogene 24:3319-27. 2005

Collaborators

Detail Information

Publications35

  1. pmc The discovery of ubiquitin-dependent proteolysis
    Keith D Wilkinson
    Department of Biochemistry, Emory University School of Medicine, 4017 Rollins Research Building, 1510 Clifton Road, Atlanta, GA 30322, USA
    Proc Natl Acad Sci U S A 102:15280-2. 2005
    ..The story of this discovery is a textbook example of the confluence of intellectual curiosity, unselfish collaboration, chance, luck, and preparation...
  2. pmc The ubiquitin signal: assembly, recognition and termination. Symposium on ubiquitin and signaling
    Keith D Wilkinson
    Department of Biochemistry, 4017 Rollins Research Building, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    EMBO Rep 6:815-20. 2005
  3. ncbi request reprint Ubiquitin: a Nobel protein
    Keith D Wilkinson
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Cell 119:741-5. 2004
  4. pmc Recognition of polyubiquitin isoforms by the multiple ubiquitin binding modules of isopeptidase T
    Francisca E Reyes-Turcu
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 283:19581-92. 2008
    ..These data suggest that recognition of the polyubiquitin isoforms by IsoT involves considerable conformational mobility in the polyubiquitin ligand, in the enzyme, or in both...
  5. pmc Prion induction by the short-lived, stress-induced protein Lsb2 is regulated by ubiquitination and association with the actin cytoskeleton
    Tatiana A Chernova
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Mol Cell 43:242-52. 2011
    ..These findings directly implicate the UPS and actin cytoskeleton in regulating prions via a stress-inducible QN-rich protein...
  6. pmc Doa1 is a Cdc48 adapter that possesses a novel ubiquitin binding domain
    James E Mullally
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Mol Cell Biol 26:822-30. 2006
    ..Combined, our data suggest that Doa1 plays a physiological role as a ubiquitin binding cofactor of Cdc48 and that human PLAA may play an analogous role via its interaction with p97/VCP...
  7. ncbi request reprint Effects of ubiquitin system alterations on the formation and loss of a yeast prion
    Kim D Allen
    School of Biology and Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332 0230, USA
    J Biol Chem 282:3004-13. 2007
    ..We propose that UPS alterations induce an adaptive response, resulting in accumulation of the large "aggresome"-like aggregates that promote de novo prion generation and prion recovery from the chaperone treatment...
  8. ncbi request reprint Modulating huntingtin half-life alters polyglutamine-dependent aggregate formation and cell toxicity
    Michael D Kaytor
    Department of Human Genetics, Emory University School of Medicine, Atlanta, Georgia, USA
    J Neurochem 89:962-73. 2004
    ..Polyglutamine-induced neurotoxicity may therefore be triggered by non-aggregated protein, and aggregate formation itself may be a cellular defense mechanism...
  9. pmc Hsp70 chaperones as modulators of prion life cycle: novel effects of Ssa and Ssb on the Saccharomyces cerevisiae prion [PSI+]
    Kim D Allen
    School of Biology and Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, 30332 0363, USA
    Genetics 169:1227-42. 2005
    ..In contrast, Ssb either stimulates refolding into nonprion conformation or targets misfolded proteins for degradation, in this way counteracting prion formation and propagation...
  10. ncbi request reprint Ubiquitin-proteasome degradation of KLF5 transcription factor in cancer and untransformed epithelial cells
    Ceshi Chen
    Department of Oncology and Hematology, Winship Cancer Institute, Emory University School of Medicine, 1365 C Clifton Road, Atlanta, GA, USA
    Oncogene 24:3319-27. 2005
    ..These results suggest that KLF5 protein is degraded at least in part through ubiquitination-proteasome pathway, which may have become hyperactive for KLF5 in cancer cells...
  11. pmc Protein partners of deubiquitinating enzymes
    Karen H Ventii
    Department of Biochemistry, Rollins Research Building, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    Biochem J 414:161-75. 2008
    ..In the present review we suggest that, like ligases, much of the regulation and specificity of deubiquitination arises from the association of DUBs with these protein partners...
  12. pmc BRCA1-associated protein-1 is a tumor suppressor that requires deubiquitinating activity and nuclear localization
    Karen H Ventii
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Cancer Res 68:6953-62. 2008
    ..The identification of the deubiquitinating enzyme BAP1 as a tumor suppressor may lead to further understanding of how the ubiquitin proteasome system contributes to cancer and aid in the identification of new targets for cancer therapy...
  13. ncbi request reprint The ubiquitin binding domain ZnF UBP recognizes the C-terminal diglycine motif of unanchored ubiquitin
    Francisca E Reyes-Turcu
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Cell 124:1197-208. 2006
    ..These data suggest that binding the ubiquitin C terminus may be necessary for the function of other proteins...
  14. pmc Distribution and paralogue specificity of mammalian deSUMOylating enzymes
    Nagamalleswari Kolli
    Department of Biochemistry, Emory University, Atlanta, GA 30322, U S A
    Biochem J 430:335-44. 2010
    ..Our data suggest that SUMO2 metabolism is more dynamic than that of SUMO1 since most SENPs display a marked preference for SUMO2...
  15. ncbi request reprint Familial Parkinson's disease-associated L166P mutation disrupts DJ-1 protein folding and function
    James A Olzmann
    Departments of Pharmacology, Biochemistry, and Neurology, Center for Neurodegenerative Disease, Emory University School of Medicine, Atlanta, Georgia 30322 3090, USA
    J Biol Chem 279:8506-15. 2004
    ..Together these findings provide insights into the molecular mechanism by which loss-of-function mutations in DJ-1 lead to Parkinson's disease...
  16. pmc An emerging model for BAP1's role in regulating cell cycle progression
    Ziad M Eletr
    Department of Biochemistry, Emory University, Atlanta, GA 30322, USA
    Cell Biochem Biophys 60:3-11. 2011
    ..Given the robust associations between BAP1/HCF-1 and HCF-1/E2Fs, it is reasonable to speculate that BAP1 influences cell proliferation at G1/S by co-regulating transcription from HCF-1/E2F-governed promoters...
  17. ncbi request reprint Deubiquitinating enzyme purification, assay inhibitors, and characterization
    Nathaniel S Russell
    Graduate Program in Biochemistry, Cell, and Developmental Biology, Emory University School of Medicine, Atlanta, GA, USA
    Methods Mol Biol 301:207-19. 2005
    ..coli using low-temperature expression as well as ion-exchange and affinity chromatography...
  18. pmc Dynamic compartmentalization of base excision repair proteins in response to nuclear and mitochondrial oxidative stress
    Lyra M Griffiths
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Mol Cell Biol 29:794-807. 2009
    ..These studies demonstrate that trafficking of DNA repair proteins to organelles containing high levels of oxidative DNA damage may be a central point for regulating BER in response to oxidative stress...
  19. ncbi request reprint Identification of a novel 29-linked polyubiquitin binding protein, Ufd3, using polyubiquitin chain analogues
    Nathaniel S Russell
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Biochemistry 43:4844-54. 2004
    ..Identification of these proteins will lead to a greater understanding of the physiological relevance of different polyubiquitin linkages...
  20. ncbi request reprint Pleiotropic effects of Ubp6 loss on drug sensitivities and yeast prion are due to depletion of the free ubiquitin pool
    Tatiana A Chernova
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 278:52102-15. 2003
    ..These data link the Ub system to prion expression and propagation and have broad implications for other neuronal inclusion body diseases...
  21. ncbi request reprint Derivitization of the C-terminus of ubiquitin and ubiquitin-like proteins using intein chemistry: methods and uses
    Keith D Wilkinson
    Department of Biochemistry, Emory University, Atlanta, Georgia, USA
    Methods Enzymol 399:37-51. 2005
    ..Only active enzymes are efficiently detected with these reagents. Published results indicate that specificity is not strictly defined by the evolutionary relationships of these DUBs...
  22. ncbi request reprint Quantitative analysis of protein-protein interactions
    Keith D Wilkinson
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA
    Methods Mol Biol 261:15-32. 2004
    ..This chapter discusses some common design considerations that are necessary to obtain valid measurements, as well as the assumptions and analytical methods that are relevant to the quantitation of these interactions...
  23. pmc Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages
    John Shanks
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Infect Immun 77:1636-48. 2009
    ..Although the physiological substrate(s) is currently unknown, the TssM deubiquitinase may provide B. mallei a selective advantage in the intracellular environment during infection...
  24. ncbi request reprint Identification and characterization of DEN1, a deneddylase of the ULP family
    Tudeviin Gan-Erdene
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 278:28892-900. 2003
    ..This selectivity was unexpected as DEN1 is most closely related to enzymes that catalyze desumoylation. This observation expands to four the number of DUB families with members that can process the C terminus of Nedd8...
  25. ncbi request reprint Varshavsky's contributions
    Wolfgang Baumeister
    Science 306:1290-2. 2004
  26. ncbi request reprint Cell biology: unchaining the condemned
    Keith D Wilkinson
    Nature 419:351-3. 2002
  27. ncbi request reprint Chemistry-based functional proteomics reveals novel members of the deubiquitinating enzyme family
    Anna Borodovsky
    Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Chem Biol 9:1149-59. 2002
    ..We show that this polypeptide reacts with the C terminus of Ub, thus demonstrating DUB-like enzymatic activity for this novel superfamily of proteases...
  28. pmc Characterization of ubiquitin and ubiquitin-like-protein isopeptidase activities
    Benjamin Nicholson
    Division of Research and Development, Progenra, Inc, Malvern, Pennsylvania 19355, USA
    Protein Sci 17:1035-43. 2008
    ..Taken together, these studies illustrate the utility of the reporter-based approach to measuring isopeptidase activity...
  29. pmc Structure and mechanisms of the proteasome-associated deubiquitinating enzyme USP14
    Min Hu
    Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Princeton, NJ, USA
    EMBO J 24:3747-56. 2005
    ..These structural observations, in conjunction with biochemical characterization, identify important regulatory mechanisms for USP14...
  30. ncbi request reprint DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1
    Kenneth Wu
    Derald H Ruttenberg Cancer Center and Department of Human Genetics, The Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    J Biol Chem 278:28882-91. 2003
    ..These results suggest a unique role for DEN1 in regulating the modification of cullins by Nedd8...
  31. ncbi request reprint Signal transduction: aspirin, ubiquitin and cancer
    Keith D Wilkinson
    Nature 424:738-9. 2003
  32. doi request reprint OTU takes the chains OUT
    Maxim Y Balakirev
    Nat Chem Biol 4:227-8. 2008
  33. pmc Structural basis for ubiquitin recognition by the Otu1 ovarian tumor domain protein
    Troy Eugene Messick
    Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 283:11038-49. 2008
    ..Together, these studies provide new insights into ubiquitin binding and hydrolysis by yeast Otu1 and other OTU domain-containing proteins...
  34. pmc Proteolytic processing and deubiquitinating activity of papain-like proteases of human coronavirus NL63
    Zhongbin Chen
    Department of Microbiology and Immunology, Loyola University Medical Center, 2160 South First Avenue, Bldg 105, Rm 3929, Maywood, IL 60153, USA
    J Virol 81:6007-18. 2007
    ....
  35. doi request reprint DeTEKting ubiquitination of APC/C substrates
    Arthur L Haas
    Department of Biochemistry and Molecular Biology, Louisiana State University School of Medicine, New Orleans, LA 70112, USA
    Cell 133:570-2. 2008
    ..Reporting in this issue, Jin et al. (2008) identify a TEK motif in both ubiquitin and substrates of APC/C that mediates assembly of these degradation signals...