Gregory B Melikyan
Affiliation: Emory University
Marin M, Kushnareva Y, Mason C, Chanda S, Melikyan G. HIV-1 Fusion with CD4+ T cells Is Promoted by Proteins Involved in Endocytosis and Intracellular Membrane Trafficking. Viruses. 2019;11: pubmed publisher
..Ectopic expression of Rab5A in non-permissive cells harboring Rab5A shRNAs partially restored the HIV-cell fusion. Together, these results implicate endocytic machinery in productive HIV-1 entry into CD4+ T cells. ..
Padilla Parra S, Marin M, Kondo N, Melikyan G. Pinpointing retrovirus entry sites in cells expressing alternatively spliced receptor isoforms by single virus imaging. Retrovirology. 2014;11:47 pubmed publisher
..Our findings demonstrate the ability of this retrovirus to enter cells via alternative endocytic pathways and establish infection by releasing its content from distinct endosomal compartments. ..
Kondo N, Marin M, Kim J, Desai T, Melikyan G. Distinct requirements for HIV-cell fusion and HIV-mediated cell-cell fusion. J Biol Chem. 2015;290:6558-73 pubmed publisher
..We surmise, however, that cell-cell contacts enabling HIV-1 fusion with the plasma membrane could be favored at the sites of high density of target cells, such as lymph nodes. ..
Giroud C, Marin M, Hammonds J, Spearman P, Melikyan G. P2X1 Receptor Antagonists Inhibit HIV-1 Fusion by Blocking Virus-Coreceptor Interactions. J Virol. 2015;89:9368-82 pubmed publisher
..P2X1 receptor antagonists could thus represent a new class of dual-coreceptor inhibitors with a structure and a mechanism of action that are distinct from those of known HIV-1 coreceptor antagonists. ..
Francis A, Melikyan G. Live-Cell Imaging of Early Steps of Single HIV-1 Infection. Viruses. 2018;10: pubmed publisher
..Here, we discuss single virus imaging strategies, focusing on live-cell imaging of single virus fusion and productive uncoating that culminates in HIV-1 infection. ..
Desai T, Marin M, Sood C, Shi J, Nawaz F, Aiken C, et al
. Fluorescent protein-tagged Vpr dissociates from HIV-1 core after viral fusion and rapidly enters the cell nucleus. Retrovirology. 2015;12:88 pubmed publisher
..Future studies will address the question whether the quick and efficient nuclear delivery of Vpr derived from incoming viruses can regulate subsequent steps of HIV-1 infection. ..
Oum Y, Desai T, Marin M, Melikyan G. Click labeling of unnatural sugars metabolically incorporated into viral envelope glycoproteins enables visualization of single particle fusion. J Virol Methods. 2016;233:62-71 pubmed publisher
..Incorporation of a content marker into surface-labeled viral particles enables sensitive detection of single virus fusion with live cells. ..
Francis A, Melikyan G. Single HIV-1 Imaging Reveals Progression of Infection through CA-Dependent Steps of Docking at the Nuclear Pore, Uncoating, and Nuclear Transport. Cell Host Microbe. 2018;23:536-548.e6 pubmed publisher
..Thus, CA protects HIV-1 complexes from degradation, mediates docking at the nuclear pore before uncoating, and determines the depth of nuclear penetration en route to integration. ..
Sood C, Marin M, Chande A, Pizzato M, Melikyan G. SERINC5 protein inhibits HIV-1 fusion pore formation by promoting functional inactivation of envelope glycoproteins. J Biol Chem. 2017;292:6014-6026 pubmed publisher
..The increased HIV-1 sensitivity to anti-gp41 antibodies and peptides suggests that SER5 also delays refolding of the remaining fusion-competent Env trimers. ..