Andrew Kowalczyk

Summary

Affiliation: Emory University
Country: USA

Publications

  1. ncbi request reprint VE-cadherin: adhesion at arm's length
    Peter A Vincent
    Dept of Dermatology, Emory Univ School of Medicine, Woodruff Memorial Bldg, 1639 Pierce Drive, Atlanta, GA 30322, USA
    Am J Physiol Cell Physiol 286:C987-97. 2004
  2. pmc Cellular levels of p120 catenin function as a set point for cadherin expression levels in microvascular endothelial cells
    Kanyan Xiao
    Department of Dermatology, School of Medicine, Emory University, Atlanta, GA 30322, USA
    J Cell Biol 163:535-45. 2003
  3. pmc Desmosome assembly and disassembly are membrane raft-dependent
    Sara N Stahley
    Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia, United States of America Graduate Program in Biochemistry, Cell and Developmental Biology, Emory University School of Medicine, Atlanta, Georgia, United States of America
    PLoS ONE 9:e87809. 2014
  4. pmc Signaling dependent and independent mechanisms in pemphigus vulgaris blister formation
    Masataka Saito
    Departments of Cell Biology, Dermatology and the Winship Cancer Institute, Emory University, Atlanta, Georgia, United States of America
    PLoS ONE 7:e50696. 2012
  5. pmc p120-catenin binding masks an endocytic signal conserved in classical cadherins
    Benjamin A Nanes
    Graduate Program in Biochemistry, Cell, and Developmental Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Cell Biol 199:365-80. 2012
  6. pmc Adherens junction turnover: regulating adhesion through cadherin endocytosis, degradation, and recycling
    Andrew P Kowalczyk
    Department of Cell Biology, Emory University School of Medicine, 30332, Atlanta, GA, USA
    Subcell Biochem 60:197-222. 2012
  7. ncbi request reprint VE-cadherin and desmoplakin are assembled into dermal microvascular endothelial intercellular junctions: a pivotal role for plakoglobin in the recruitment of desmoplakin to intercellular junctions
    A P Kowalczyk
    Departments of Dermatology, Pathology, and the Robert H Lurie Cancer Center, Northwestern University Medical School, Chicago, IL, USA
    J Cell Sci 111:3045-57. 1998
  8. ncbi request reprint Protecting your tail: regulation of cadherin degradation by p120-catenin
    Andrew P Kowalczyk
    Departments of Dermatology and Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Curr Opin Cell Biol 16:522-7. 2004
  9. ncbi request reprint Anthrax toxin receptor 1/tumor endothelium marker 8 mediates cell spreading by coupling extracellular ligands to the actin cytoskeleton
    Erica Werner
    Department of Cell Biology, Emory University, Atlanta, Georgia 30322 3030, USA
    J Biol Chem 281:23227-36. 2006
  10. pmc p120-catenin inhibits VE-cadherin internalization through a Rho-independent mechanism
    Christine M Chiasson
    Graduate Program in Biochemistry, Cell, and Developmental Biology, and Department of Cell Biology, Emory University, Atlanta, GA 30322, USA
    Mol Biol Cell 20:1970-80. 2009

Research Grants

Collaborators

  • Kathleen M Buckley
  • KATHLEEN JANEE GREEN
  • Kanyan Xiao
  • E Dejana
  • Albert B Reynolds
  • Gloria Salazar
  • JONATHAN C contact JONES
  • ERICA MARLIS WERNER
  • Masayuki Amagai
  • Mechthild Hatzfeld
  • Hans Grossniklaus
  • A Ishiko
  • Victor Faundez
  • Emmanuella Delva
  • Catharine C Calkins
  • Peter A Vincent
  • Melanie L Styers
  • Sara N Stahley
  • Masataka Saito
  • Susan Summers
  • Taofei Yin
  • Christine M Chiasson
  • Shannon V Setzer
  • Margaret D Kottke
  • Benjamin A Nanes
  • Dana K Tucker
  • Rebecca G Oas
  • Kristin B Wittich
  • Amy K Gira
  • Joseph L Charest
  • Nikhil M Urs
  • Jean Marie Jennings
  • Julie M Anderton
  • Spiro Getsios
  • Eliane J Muller
  • Reto Caldelari
  • Xing Cheng
  • Alexa L Mattheyses
  • Kala Venkiteswaran
  • Michael Koval
  • Anthony M Lowery
  • Christine Chiasson-Mackenzie
  • Noboru Ishiyama
  • Xuming Mao
  • Aimee S Payne
  • Mitsuhiko Ikura
  • Christopher Y Caughman
  • W David Martin
  • Andres J Garcia
  • Jean M Jennings
  • Robert A Swerlick
  • William P King
  • Yue Feng
  • Harish Radhakrishna
  • Edwin W Ades
  • Gowrisankar Rajam
  • George M Carlone
  • Sandra Romero-Steiner
  • Jacquelyn S Sampson
  • Susan Summer
  • Kazuyuki Tsunoda
  • Lisa M Godsel
  • Peter J Koch
  • Jennifer Garner
  • Rachal Love
  • Zhining Den
  • Andrew A Peden
  • Atsushi Shimizu
  • Kusal Mihindukulasuriya
  • Matthew R Novak
  • Bridgett L Hoepner
  • Christine M Law
  • Kevin Pumiglia

Detail Information

Publications33

  1. ncbi request reprint VE-cadherin: adhesion at arm's length
    Peter A Vincent
    Dept of Dermatology, Emory Univ School of Medicine, Woodruff Memorial Bldg, 1639 Pierce Drive, Atlanta, GA 30322, USA
    Am J Physiol Cell Physiol 286:C987-97. 2004
    ..These recent studies place p120(ctn) at the hub of a cadherin-catenin regulatory mechanism that controls cadherin plasma membrane levels in cells of both epithelial and endothelial origin...
  2. pmc Cellular levels of p120 catenin function as a set point for cadherin expression levels in microvascular endothelial cells
    Kanyan Xiao
    Department of Dermatology, School of Medicine, Emory University, Atlanta, GA 30322, USA
    J Cell Biol 163:535-45. 2003
    ..These results demonstrate that cellular levels of p120ctn function as a set point mechanism that regulates cadherin expression levels, and that a major function of p120ctn is to control cadherin internalization and degradation...
  3. pmc Desmosome assembly and disassembly are membrane raft-dependent
    Sara N Stahley
    Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia, United States of America Graduate Program in Biochemistry, Cell and Developmental Biology, Emory University School of Medicine, Atlanta, Georgia, United States of America
    PLoS ONE 9:e87809. 2014
    ..These findings demonstrate that membrane rafts are required for desmosome assembly and disassembly dynamics, suggesting therapeutic potential for raft targeting agents in desmosomal diseases such as PV. ..
  4. pmc Signaling dependent and independent mechanisms in pemphigus vulgaris blister formation
    Masataka Saito
    Departments of Cell Biology, Dermatology and the Winship Cancer Institute, Emory University, Atlanta, Georgia, United States of America
    PLoS ONE 7:e50696. 2012
    ..These findings have important implications for understanding pemphigus pathophysiology, and for the design of pemphigus model systems and therapeutic interventions...
  5. pmc p120-catenin binding masks an endocytic signal conserved in classical cadherins
    Benjamin A Nanes
    Graduate Program in Biochemistry, Cell, and Developmental Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Cell Biol 199:365-80. 2012
    ..These results reveal the mechanistic basis by which p120 stabilizes cadherins and demonstrate that VE-cadherin endocytosis is crucial for endothelial cell migration in response to an angiogenic growth factor...
  6. pmc Adherens junction turnover: regulating adhesion through cadherin endocytosis, degradation, and recycling
    Andrew P Kowalczyk
    Department of Cell Biology, Emory University School of Medicine, 30332, Atlanta, GA, USA
    Subcell Biochem 60:197-222. 2012
    ..Lastly, we discuss the proteolytic cleavage of cadherins at the plasma membrane...
  7. ncbi request reprint VE-cadherin and desmoplakin are assembled into dermal microvascular endothelial intercellular junctions: a pivotal role for plakoglobin in the recruitment of desmoplakin to intercellular junctions
    A P Kowalczyk
    Departments of Dermatology, Pathology, and the Robert H Lurie Cancer Center, Northwestern University Medical School, Chicago, IL, USA
    J Cell Sci 111:3045-57. 1998
    ..These results suggest that endothelial cells assemble unique junctional complexes that couple VE-cadherin to both the actin and intermediate filament cytoskeleton...
  8. ncbi request reprint Protecting your tail: regulation of cadherin degradation by p120-catenin
    Andrew P Kowalczyk
    Departments of Dermatology and Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Curr Opin Cell Biol 16:522-7. 2004
    ....
  9. ncbi request reprint Anthrax toxin receptor 1/tumor endothelium marker 8 mediates cell spreading by coupling extracellular ligands to the actin cytoskeleton
    Erica Werner
    Department of Cell Biology, Emory University, Atlanta, Georgia 30322 3030, USA
    J Biol Chem 281:23227-36. 2006
    ..Thus, our results indicate that TEM8 is a new adhesion molecule linking collagen I or PA to the actin cytoskeleton...
  10. pmc p120-catenin inhibits VE-cadherin internalization through a Rho-independent mechanism
    Christine M Chiasson
    Graduate Program in Biochemistry, Cell, and Developmental Biology, and Department of Cell Biology, Emory University, Atlanta, GA 30322, USA
    Mol Biol Cell 20:1970-80. 2009
    ....
  11. pmc p120-Catenin regulates clathrin-dependent endocytosis of VE-cadherin
    Kanyan Xiao
    Department of Dermatology, Emory University, Atlanta, GA 30322, USA
    Mol Biol Cell 16:5141-51. 2005
    ....
  12. ncbi request reprint Role of p120-catenin in cadherin trafficking
    Kanyan Xiao
    Department of Cell Biology and Dermatology, Emory University School of Medicine, Whitehead Biomedical Research Building, 615 Michael Street, Atlanta, GA 30322, USA
    Biochim Biophys Acta 1773:8-16. 2007
    ..Through these mechanisms p120 exerts influence over a wide range of biological processes that are dependent upon tight regulation of cell surface cadherin levels...
  13. ncbi request reprint Desmoglein endocytosis and desmosome disassembly are coordinated responses to pemphigus autoantibodies
    Catharine C Calkins
    Departments of Dermatology and Cell Biology, Emory University School of Medicine, Whitehead Biomedical Research Building, 615 Michael Street, Atlanta, GA 30322, USA
    J Biol Chem 281:7623-34. 2006
    ..These findings demonstrate that Dsg3 endocytosis, keratin filament retraction, and the loss of keratinocyte cell-cell adhesion are coordinated responses to PV IgG...
  14. ncbi request reprint Regulation of endothelial barrier function and growth by VE-cadherin, plakoglobin, and beta-catenin
    Kala Venkiteswaran
    Department of Dermatology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Am J Physiol Cell Physiol 283:C811-21. 2002
    ..These observations suggest that intercellular junction proteins function as components of an adhesion and signaling system that regulates vascular barrier function and growth...
  15. ncbi request reprint The Armadillo family protein p0071 is a VE-cadherin- and desmoplakin-binding protein
    Catharine C Calkins
    Department of Dermatology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 278:1774-83. 2003
    ....
  16. ncbi request reprint Intermediate filaments and vesicular membrane traffic: the odd couple's first dance?
    Melanie L Styers
    Department of Cell Biology, Emory University, Atlanta, GA, USA
    Traffic 6:359-65. 2005
    ..Furthermore, we will discuss potential mechanisms by which IFs may regulate the localization and function of organelles...
  17. ncbi request reprint Mechanisms of VE-cadherin processing and degradation in microvascular endothelial cells
    Kanyan Xiao
    Department of Dermatology and Emory Skin Diseases Research Center, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 278:19199-208. 2003
    ..These results provide new insights into the mechanisms of VE-cadherin processing and degradation in microvascular endothelial cells...
  18. pmc Regulation of cadherin trafficking
    Emmanuella Delva
    Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30332, USA
    Traffic 10:259-67. 2009
    ..Particular emphasis is placed on the regulation of cadherin trafficking by catenins and the interplay between growth factor signaling pathways and cadherin endocytosis...
  19. ncbi request reprint Architecture of the vimentin cytoskeleton is modified by perturbation of the GTPase ARF1
    Melanie L Styers
    Graduate Program in Biochemistry, Cell, and Developmental Biology, Emory University, Atlanta, GA 30322, USA
    J Cell Sci 119:3643-54. 2006
    ..These observations indicate that perturbation of the vesicular membrane transport machinery lead to reciprocal changes in the architecture of vimentin networks...
  20. ncbi request reprint Mechanisms of plakoglobin-dependent adhesion: desmosome-specific functions in assembly and regulation by epidermal growth factor receptor
    Taofei Yin
    Department of Pathology and Dermatology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611, USA
    J Biol Chem 280:40355-63. 2005
    ..As a target for modulation by EGF, regulation of PG-dependent adhesion may play an important role during wound healing and tumor metastasis...
  21. pmc Plakoglobin suppresses keratinocyte motility through both cell-cell adhesion-dependent and -independent mechanisms
    Taofei Yin
    Departments of Pathology and Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, 303 East Chicago Avenue, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 102:5420-5. 2005
    ....
  22. pmc p120-Catenin is required for mouse vascular development
    Rebecca G Oas
    Department of Cell Biology, 615 Michael St, Room 465, Atlanta, GA 30322, USA
    Circ Res 106:941-51. 2010
    ..p120-catenin (p120) is an armadillo family protein that binds to the cytoplasmic domain of classical cadherins and prevents cadherin endocytosis. The role of p120 in vascular development is unknown...
  23. pmc Pemphigus vulgaris IgG-induced desmoglein-3 endocytosis and desmosomal disassembly are mediated by a clathrin- and dynamin-independent mechanism
    Emmanuella Delva
    Graduate Program in Biochemistry, Cell, and Developmental Biology, Department of Cell Biology, Emory University, Atlanta, GA 30332, USA
    J Biol Chem 283:18303-13. 2008
    ..Altogether, these results suggest that PV IgG-induced Dsg3 internalization is mediated through a clathrin- and dynamin-independent pathway and that Dsg3 endocytosis is tightly coupled to the pathogenic activity of PV IgG...
  24. ncbi request reprint Comparative analysis of armadillo family proteins in the regulation of a431 epithelial cell junction assembly, adhesion and migration
    Shannon V Setzer
    Departments of Dermatology and Cell Biology, Emory University School of Medicine, 101 Woodruff Circle, Atlanta, GA 30322, USA
    J Invest Dermatol 123:426-33. 2004
    ..These results suggest that p120ctn/plakophilin family proteins interact with intercellular junction binding partners to differentially modulate the adhesive and migratory behavior of epithelial cells...
  25. pmc The desmosome
    Emmanuella Delva
    Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Cold Spring Harb Perspect Biol 1:a002543. 2009
    ..This article outlines the structure and function of the major desmosomal proteins, and explores the contributions of this protein complex to tissue architecture and morphogenesis...
  26. ncbi request reprint Photoreceptor cells in flies and mammals: Crumby homology?
    Andrew P Kowalczyk
    Department of Dermatology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Dev Cell 2:253-4. 2002
    ..This supports the previous observation that disruption of CRB1 function can cause retinal degeneration in humans...
  27. doi request reprint Iron chelators and hypoxia mimetics inhibit IFNgamma-mediated Jak-STAT signaling
    Amy K Gira
    Department of Dermatology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Invest Dermatol 129:723-9. 2009
    ..These data suggest that the target for chelator effects may be through iron requirements for oxygen-requiring dioxygenase enzymes...
  28. pmc Cadherin-mediated cell-cell contact regulates keratinocyte differentiation
    Joseph L Charest
    George W Woodruff School of Mechanical Engineering, Atlanta, GA, USA
    J Invest Dermatol 129:564-72. 2009
    ..These results demonstrate that cadherin-mediated cell-cell contact regulates early keratinocyte differentiation independently from changes in cell shape...
  29. ncbi request reprint Different mechanisms regulate lysophosphatidic acid (LPA)-dependent versus phorbol ester-dependent internalization of the LPA1 receptor
    Nikhil M Urs
    School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    J Biol Chem 283:5249-57. 2008
    ..These results indicate that distinct molecular mechanisms regulate agonist-dependent and PMA-dependent internalization of the LPA 1 receptor...
  30. ncbi request reprint E-cadherin is a receptor for the common protein pneumococcal surface adhesin A (PsaA) of Streptococcus pneumoniae
    Julie M Anderton
    Division of Bacterial Diseases, National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Bldg 18 B104, M S G 05, Atlanta, GA 30333, USA
    Microb Pathog 42:225-36. 2007
    ..This work identifies E-cadherin as a receptor on human epithelial cells for the pneumococcal surface adhesin, PsaA...
  31. pmc The endo-lysosomal sorting machinery interacts with the intermediate filament cytoskeleton
    Melanie L Styers
    Graduate Division of Biological and Biomedical Sciences, Emory University, Atlanta, GA 30322, USA
    Mol Biol Cell 15:5369-82. 2004
    ..Our results reveal a novel role of the intermediate filament cytoskeleton in organelle/adaptor positioning and in regulation of the adaptor complex AP-3...
  32. ncbi request reprint The desmosome: cell science lessons from human diseases
    Margaret D Kottke
    Department of Dermatology, Emory University School of Medicine, Emory University, Atlanta, GA 30322, USA
    J Cell Sci 119:797-806. 2006
    ..These human diseases reveal key roles for desmosomes in maintaining tissue integrity, but also suggest functions for desmosomal components in signal transduction pathways and epidermal organization...
  33. pmc Assessment of splice variant-specific functions of desmocollin 1 in the skin
    Xing Cheng
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 24:154-63. 2004
    ..However, a comparison of our mutants with dsc1-null mice suggests that the Dsc1 extracellular domain is necessary to maintain structural integrity of the skin...

Research Grants17

  1. Role of p0071 in Cutaneous Intercellular Junctions
    Andrew Kowalczyk; Fiscal Year: 2006
    ..abstract_text> ..
  2. Role of p0071 in Cutaneous Intercellular Junctions
    Andrew Kowalczyk; Fiscal Year: 2002
    ..abstract_text> ..
  3. Mechanisms of Desmosome Regulation and Disassembly in the Skin Disease Pemphigus
    Andrew P Kowalczyk; Fiscal Year: 2010
    ....
  4. Cadherin Regulation in Dermal Endothelial Cells
    Andrew Kowalczyk; Fiscal Year: 2009
    ....
  5. Mechanisms of Desmosome Regulation and Disassembly in the Skin Disease Pemphigus
    Andrew Kowalczyk; Fiscal Year: 2007
    ..abstract_text> ..
  6. Cadherin Regulation in Dermal Endothelial Cells
    Andrew Kowalczyk; Fiscal Year: 2007
    ..abstract_text> ..
  7. Cadherin Regulation in Dermal Endothelial Cells
    Andrew Kowalczyk; Fiscal Year: 2006
    ..abstract_text> ..
  8. Cadherin Regulation in Dermal Endothelial Cells
    Andrew Kowalczyk; Fiscal Year: 2005
    ..abstract_text> ..
  9. Role of p0071 in Cutaneous Intercellular Junctions
    Andrew Kowalczyk; Fiscal Year: 2005
    ..abstract_text> ..
  10. Cadherin Regulation in Dermal Endothelial Cells
    Andrew Kowalczyk; Fiscal Year: 2004
    ..abstract_text> ..
  11. Pemphigus IgG Internalization and Desmosome Disassembly
    Andrew Kowalczyk; Fiscal Year: 2004
    ..The long-term goal of these studies is to expose new cellular pathways that might be targeted therapeutically to make keratinocytes more resistant to PV IgG in patients suffering from this devastating disease. ..
  12. Role of p0071 in Cutaneous Intercellular Junctions
    Andrew Kowalczyk; Fiscal Year: 2004
    ..abstract_text> ..
  13. Role of p0071 in Cutaneous Intercellular Junctions
    Andrew Kowalczyk; Fiscal Year: 2003
    ..abstract_text> ..
  14. Cadherin Regulation in Dermal Endothelial Cells
    Andrew P Kowalczyk; Fiscal Year: 2010
    ....
  15. Mechanisms of Desmosome Regulation and Disassembly in the Skin Disease Pemphigus
    Andrew Kowalczyk; Fiscal Year: 2009
    ....