Tongzhong Ju

Summary

Affiliation: Emory University
Country: USA

Publications

  1. ncbi request reprint Purification, characterization, and subunit structure of rat core 1 Beta1,3-galactosyltransferase
    Tongzhong Ju
    W K Warren Medical Research Institute, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 277:169-77. 2002
  2. ncbi request reprint Cloning and expression of human core 1 beta1,3-galactosyltransferase
    Tongzhong Ju
    W K Warren Medical Research Institute, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 277:178-86. 2002
  3. pmc A unique molecular chaperone Cosmc required for activity of the mammalian core 1 beta 3-galactosyltransferase
    Tongzhong Ju
    Department of Biochemistry and Molecular Biology and the Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Proc Natl Acad Sci U S A 99:16613-8. 2002
  4. ncbi request reprint Protein glycosylation: chaperone mutation in Tn syndrome
    Tongzhong Ju
    Department of Biochemistry and Molecular Biology, and Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Nature 437:1252. 2005
  5. ncbi request reprint Identification of core 1 O-glycan T-synthase from Caenorhabditis elegans
    Tongzhong Ju
    Department of Biochemistry and Molecular Biology, Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Glycobiology 16:947-58. 2006
  6. pmc A novel fluorescent assay for T-synthase activity
    Tongzhong Ju
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Glycobiology 21:352-62. 2011
  7. doi request reprint Human tumor antigens Tn and sialyl Tn arise from mutations in Cosmc
    Tongzhong Ju
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Cancer Res 68:1636-46. 2008
  8. pmc Regulation of protein O-glycosylation by the endoplasmic reticulum-localized molecular chaperone Cosmc
    Tongzhong Ju
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Cell Biol 182:531-42. 2008
  9. pmc Tight complex formation between Cosmc chaperone and its specific client non-native T-synthase leads to enzyme activity and client-driven dissociation
    Rajindra P Aryal
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 287:15317-29. 2012
  10. pmc Platelet biogenesis and functions require correct protein O-glycosylation
    Yingchun Wang
    Departments of Biochemistry and Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA
    Proc Natl Acad Sci U S A 109:16143-8. 2012

Research Grants

  1. Genetic Basis of Non-Familial IgA Nephropathy
    Tongzhong Ju; Fiscal Year: 2009
  2. Genetic Basis of Non-Familial IgA Nephropathy
    Tongzhong Ju; Fiscal Year: 2010

Collaborators

  • Rosemary E Zuna
  • Rodger P McEver
  • Vivianne I Otto
  • Lijun Xia
  • David F Smith
  • Qian Sun
  • Richard D Cummings
  • Rajindra P Aryal
  • Yingchun Wang
  • Sean R Stowell
  • Rongjuan Mi
  • Xiaokun Ding
  • Baoyun Xia
  • Connie M Arthur
  • Lilian C Rodrigues
  • Marcelo Dias-Baruffi
  • Guangyu An
  • Hyojung Choo
  • Arlene B Chapman
  • Shawn M Jobe
  • Sylvain Lehoux
  • Irma van Die
  • Jianmei Wang
  • David R Archer
  • Vanja K Crew
  • Lina Song
  • Junwei Zeng
  • Carlos Rivera-Marrero
  • Ross J Molinaro
  • Miao He
  • Jamie Heimburg-Molinaro
  • Wenyi Wang
  • Jean Philippe Gourdine
  • Jonathan Miner
  • Sougata Karmakar
  • Thalita B Riul
  • Bo Wei
  • J Michael McDaniel
  • Jonathan Braun

Detail Information

Publications23

  1. ncbi request reprint Purification, characterization, and subunit structure of rat core 1 Beta1,3-galactosyltransferase
    Tongzhong Ju
    W K Warren Medical Research Institute, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 277:169-77. 2002
    ..These studies demonstrate that activity of the core 1 beta1,3-Gal-T from rat liver is contained within a single, novel, disulfide-bonded, dimeric enzyme...
  2. ncbi request reprint Cloning and expression of human core 1 beta1,3-galactosyltransferase
    Tongzhong Ju
    W K Warren Medical Research Institute, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 277:178-86. 2002
    ..The core 1 beta3-Gal-T sequence has minimal homology with conserved sequences found in previously described beta3-galactosyltransferases, suggesting this enzyme is only distantly related to the known beta3-galactosyltransferase family...
  3. pmc A unique molecular chaperone Cosmc required for activity of the mammalian core 1 beta 3-galactosyltransferase
    Tongzhong Ju
    Department of Biochemistry and Molecular Biology and the Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Proc Natl Acad Sci U S A 99:16613-8. 2002
    ....
  4. ncbi request reprint Protein glycosylation: chaperone mutation in Tn syndrome
    Tongzhong Ju
    Department of Biochemistry and Molecular Biology, and Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Nature 437:1252. 2005
    ....
  5. ncbi request reprint Identification of core 1 O-glycan T-synthase from Caenorhabditis elegans
    Tongzhong Ju
    Department of Biochemistry and Molecular Biology, Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Glycobiology 16:947-58. 2006
    ..These results show that the Ce-T-synthase is a functional ortholog to the human T-synthase in generating core 1 O-glycans and open new avenues to explore O-glycan function in this model organism...
  6. pmc A novel fluorescent assay for T-synthase activity
    Tongzhong Ju
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Glycobiology 21:352-62. 2011
    ..This new high-throughput assay allows for facile screening of tumor specimens and other biological material for T-synthase activity and could be used diagnostically...
  7. doi request reprint Human tumor antigens Tn and sialyl Tn arise from mutations in Cosmc
    Tongzhong Ju
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Cancer Res 68:1636-46. 2008
    ..This is the first example of somatic mutations in multiple types of cancers that cause global alterations in cell surface carbohydrate antigen expression...
  8. pmc Regulation of protein O-glycosylation by the endoplasmic reticulum-localized molecular chaperone Cosmc
    Tongzhong Ju
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Cell Biol 182:531-42. 2008
    ....
  9. pmc Tight complex formation between Cosmc chaperone and its specific client non-native T-synthase leads to enzyme activity and client-driven dissociation
    Rajindra P Aryal
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 287:15317-29. 2012
    ..Taken together, these results demonstrate a novel mechanism in which Cosmc cycles to bind non-native T-synthase, leading to enzyme activity and release in a client-driven process...
  10. pmc Platelet biogenesis and functions require correct protein O-glycosylation
    Yingchun Wang
    Departments of Biochemistry and Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA
    Proc Natl Acad Sci U S A 109:16143-8. 2012
    ....
  11. pmc Cosmc is an essential chaperone for correct protein O-glycosylation
    Yingchun Wang
    Departments of Biochemistry and Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA
    Proc Natl Acad Sci U S A 107:9228-33. 2010
    ..We generated genetically mosaic mice with a targeted Cosmc deletion and survivors exhibited abnormalities correlated with Tn antigen expression that are related to several human diseases...
  12. pmc The endoplasmic reticulum chaperone Cosmc directly promotes in vitro folding of T-synthase
    Rajindra P Aryal
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 285:2456-62. 2010
    ..Cosmc represents the first ER chaperone identified to be required for folding of a glycosyltransferase...
  13. pmc The transmembrane domain of the molecular chaperone Cosmc directs its localization to the endoplasmic reticulum
    Qian Sun
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 286:11529-42. 2011
    ..These studies reveal that Cosmc has a unique ER-retention motif within its TMD and provide new insights into the molecular mechanisms by which TMDs of resident ER proteins contribute to ER localization...
  14. pmc Epigenetic silencing of the chaperone Cosmc in human leukocytes expressing tn antigen
    Rongjuan Mi
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 287:41523-33. 2012
    ....
  15. pmc Innate immune lectins kill bacteria expressing blood group antigen
    Sean R Stowell
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, USA
    Nat Med 16:295-301. 2010
    ..These results demonstrate that innate defense lectins can provide immunity against pathogens that express blood group-like antigens on their surface...
  16. ncbi request reprint Identification of a novel protein binding motif within the T-synthase for the molecular chaperone Cosmc
    Rajindra P Aryal
    From the Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322
    J Biol Chem 289:11630-41. 2014
    ....
  17. doi request reprint Functional assays for the molecular chaperone cosmc
    Tongzhong Ju
    Department of Biochemistry, Emory University School of Medicine, Rollins Research Center, Atlanta, Georgia, USA
    Methods Enzymol 479:107-22. 2010
    ..The methods described here allow the functional activities of such mutated Cosmc (mCosmc) to be measured and compared to wild-type (wtCosmc)...
  18. doi request reprint The Tn antigen-structural simplicity and biological complexity
    Tongzhong Ju
    Department of Biochemistry, Emory University School of Medicine, O Wayne Rollins Research Center, 1510 Clifton Road, Suite 4001, Atlanta, GA 30322, USA
    Angew Chem Int Ed Engl 50:1770-91. 2011
    ..Because of the association of the Tn antigen with disease, there is much interest in the development of Tn-based vaccines and other therapeutic approaches based on Tn expression...
  19. doi request reprint The Cosmc connection to the Tn antigen in cancer
    Tongzhong Ju
    Department of Biochemistry and the Emory Glycomics Center, Emory University School of Medicine, Atlanta, GA, USA
    Cancer Biomark 14:63-81. 2014
    ..Thus, expression of normal O-glycans is associated with health and homeostasis whereas truncation of O-glycans, e.g. the Tn and/or Sialyl Tn antigens is associated with cancer and other pathologies. ..
  20. doi request reprint A fluorescence-based assay for Core 1 β3galactosyltransferase (T-synthase) activity
    Tongzhong Ju
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, USA
    Methods Mol Biol 1022:15-28. 2013
    ..6-10 and can be easily measured by a fluorescent detector (Ex: 355 nm; Em: 460 nm). This fluorescence-based T-synthase assay is simple, sensitive, reproducible, not affected by enzyme source, and adaptable for high-throughput assays...
  21. pmc Galectin-1 induces reversible phosphatidylserine exposure at the plasma membrane
    Sean R Stowell
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Mol Biol Cell 20:1408-18. 2009
    ..These results demonstrate that Gal-1-induced PS exposure and subsequent phagocytic removal of living cells represents a new paradigm in cellular turnover...
  22. pmc Defective angiogenesis and fatal embryonic hemorrhage in mice lacking core 1-derived O-glycans
    Lijun Xia
    Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, 825 N E 13th St, Oklahoma City, OK 73104, USA
    J Cell Biol 164:451-9. 2004
    ..These data reveal an unexpected requirement for core 1-derived O-glycans during angiogenesis...
  23. pmc Increased susceptibility to colitis and colorectal tumors in mice lacking core 3-derived O-glycans
    Guangyu An
    Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    J Exp Med 204:1417-29. 2007
    ..Moreover, these mice were highly susceptible to experimental triggers of colitis and colorectal adenocarcinoma. These data reveal a requirement for core 3-derived O-glycans in resistance to colonic disease...

Research Grants3

  1. Genetic Basis of Non-Familial IgA Nephropathy
    Tongzhong Ju; Fiscal Year: 2009
    ....
  2. Genetic Basis of Non-Familial IgA Nephropathy
    Tongzhong Ju; Fiscal Year: 2010
    ....