Tongzhong Ju

Summary

Affiliation: Emory University
Country: USA

Publications

  1. ncbi Purification, characterization, and subunit structure of rat core 1 Beta1,3-galactosyltransferase
    Tongzhong Ju
    W. K. Warren Medical Research Institute, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 277:169-77. 2002
  2. ncbi Cloning and expression of human core 1 beta1,3-galactosyltransferase
    Tongzhong Ju
    W K Warren Medical Research Institute, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 277:178-86. 2002
  3. ncbi A unique molecular chaperone Cosmc required for activity of the mammalian core 1 beta 3-galactosyltransferase
    Tongzhong Ju
    Department of Biochemistry and Molecular Biology and the Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Proc Natl Acad Sci U S A 99:16613-8. 2002
  4. ncbi Protein glycosylation: chaperone mutation in Tn syndrome
    Tongzhong Ju
    Department of Biochemistry and Molecular Biology, and Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Nature 437:1252. 2005
  5. ncbi Identification of core 1 O-glycan T-synthase from Caenorhabditis elegans
    Tongzhong Ju
    Department of Biochemistry and Molecular Biology, Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Glycobiology 16:947-58. 2006
  6. ncbi A novel fluorescent assay for T-synthase activity
    Tongzhong Ju
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Glycobiology 21:352-62. 2011
  7. ncbi Human tumor antigens Tn and sialyl Tn arise from mutations in Cosmc
    Tongzhong Ju
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Cancer Res 68:1636-46. 2008
  8. ncbi Regulation of protein O-glycosylation by the endoplasmic reticulum-localized molecular chaperone Cosmc
    Tongzhong Ju
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Cell Biol 182:531-42. 2008
  9. ncbi Tight complex formation between Cosmc chaperone and its specific client non-native T-synthase leads to enzyme activity and client-driven dissociation
    Rajindra P Aryal
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 287:15317-29. 2012
  10. ncbi Platelet biogenesis and functions require correct protein O-glycosylation
    Yingchun Wang
    Departments of Biochemistry and Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA
    Proc Natl Acad Sci U S A 109:16143-8. 2012

Research Grants

  1. Genetic Basis of Non-Familial IgA Nephropathy
    Tongzhong Ju; Fiscal Year: 2009
  2. Genetic Basis of Non-Familial IgA Nephropathy
    Tongzhong Ju; Fiscal Year: 2010

Collaborators

  • Rodger P McEver
  • Vivianne I Otto
  • Lijun Xia
  • David F Smith
  • Qian Sun
  • Richard D Cummings
  • Yingchun Wang
  • Rajindra P Aryal
  • Sean R Stowell
  • Rongjuan Mi
  • Xiaokun Ding
  • Baoyun Xia
  • Connie M Arthur
  • Lilian C Rodrigues
  • Marcelo Dias-Baruffi
  • Guangyu An
  • Shawn M Jobe
  • Sylvain Lehoux
  • Hyojung Choo
  • Jianmei Wang
  • Vanja K Crew
  • Irma van Die
  • David R Archer
  • Arlene B Chapman
  • Junwei Zeng
  • Lina Song
  • Wenyi Wang
  • Ross J Molinaro
  • Carlos Rivera-Marrero
  • Jamie Heimburg-Molinaro
  • Jean Philippe Gourdine
  • Miao He
  • Jonathan Miner
  • Sougata Karmakar
  • Thalita B Riul
  • J Michael McDaniel
  • Bo Wei
  • Jonathan Braun

Detail Information

Publications20

  1. ncbi Purification, characterization, and subunit structure of rat core 1 Beta1,3-galactosyltransferase
    Tongzhong Ju
    W. K. Warren Medical Research Institute, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 277:169-77. 2002
    ..These studies demonstrate that activity of the core 1 beta1,3-Gal-T from rat liver is contained within a single, novel, disulfide-bonded, dimeric enzyme...
  2. ncbi Cloning and expression of human core 1 beta1,3-galactosyltransferase
    Tongzhong Ju
    W K Warren Medical Research Institute, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 277:178-86. 2002
    ..The core 1 beta3-Gal-T sequence has minimal homology with conserved sequences found in previously described beta3-galactosyltransferases, suggesting this enzyme is only distantly related to the known beta3-galactosyltransferase family...
  3. ncbi A unique molecular chaperone Cosmc required for activity of the mammalian core 1 beta 3-galactosyltransferase
    Tongzhong Ju
    Department of Biochemistry and Molecular Biology and the Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Proc Natl Acad Sci U S A 99:16613-8. 2002
    ....
  4. ncbi Protein glycosylation: chaperone mutation in Tn syndrome
    Tongzhong Ju
    Department of Biochemistry and Molecular Biology, and Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Nature 437:1252. 2005
    ....
  5. ncbi Identification of core 1 O-glycan T-synthase from Caenorhabditis elegans
    Tongzhong Ju
    Department of Biochemistry and Molecular Biology, Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Glycobiology 16:947-58. 2006
    ..These results show that the Ce-T-synthase is a functional ortholog to the human T-synthase in generating core 1 O-glycans and open new avenues to explore O-glycan function in this model organism...
  6. ncbi A novel fluorescent assay for T-synthase activity
    Tongzhong Ju
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Glycobiology 21:352-62. 2011
    ..This new high-throughput assay allows for facile screening of tumor specimens and other biological material for T-synthase activity and could be used diagnostically...
  7. ncbi Human tumor antigens Tn and sialyl Tn arise from mutations in Cosmc
    Tongzhong Ju
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Cancer Res 68:1636-46. 2008
    ..This is the first example of somatic mutations in multiple types of cancers that cause global alterations in cell surface carbohydrate antigen expression...
  8. ncbi Regulation of protein O-glycosylation by the endoplasmic reticulum-localized molecular chaperone Cosmc
    Tongzhong Ju
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Cell Biol 182:531-42. 2008
    ....
  9. ncbi Tight complex formation between Cosmc chaperone and its specific client non-native T-synthase leads to enzyme activity and client-driven dissociation
    Rajindra P Aryal
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 287:15317-29. 2012
    ..Taken together, these results demonstrate a novel mechanism in which Cosmc cycles to bind non-native T-synthase, leading to enzyme activity and release in a client-driven process...
  10. ncbi Platelet biogenesis and functions require correct protein O-glycosylation
    Yingchun Wang
    Departments of Biochemistry and Pediatrics, Emory University School of Medicine, Atlanta, GA 30322, USA
    Proc Natl Acad Sci U S A 109:16143-8. 2012
    ....
  11. ncbi The endoplasmic reticulum chaperone Cosmc directly promotes in vitro folding of T-synthase
    Rajindra P Aryal
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 285:2456-62. 2010
    ..Cosmc represents the first ER chaperone identified to be required for folding of a glycosyltransferase...
  12. ncbi Cosmc is an essential chaperone for correct protein O-glycosylation
    Yingchun Wang
    Departments of Biochemistry and Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA
    Proc Natl Acad Sci U S A 107:9228-33. 2010
    ..We generated genetically mosaic mice with a targeted Cosmc deletion and survivors exhibited abnormalities correlated with Tn antigen expression that are related to several human diseases...
  13. ncbi The transmembrane domain of the molecular chaperone Cosmc directs its localization to the endoplasmic reticulum
    Qian Sun
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 286:11529-42. 2011
    ..These studies reveal that Cosmc has a unique ER-retention motif within its TMD and provide new insights into the molecular mechanisms by which TMDs of resident ER proteins contribute to ER localization...
  14. ncbi Epigenetic silencing of the chaperone Cosmc in human leukocytes expressing tn antigen
    Rongjuan Mi
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 287:41523-33. 2012
    ....
  15. ncbi Innate immune lectins kill bacteria expressing blood group antigen
    Sean R Stowell
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia, USA
    Nat Med 16:295-301. 2010
    ..These results demonstrate that innate defense lectins can provide immunity against pathogens that express blood group-like antigens on their surface...
  16. ncbi The Tn antigen-structural simplicity and biological complexity
    Tongzhong Ju
    Department of Biochemistry, Emory University School of Medicine, O Wayne Rollins Research Center, 1510 Clifton Road, Suite 4001, Atlanta, GA 30322, USA
    Angew Chem Int Ed Engl 50:1770-91. 2011
    ..Because of the association of the Tn antigen with disease, there is much interest in the development of Tn-based vaccines and other therapeutic approaches based on Tn expression...
  17. ncbi Functional assays for the molecular chaperone cosmc
    Tongzhong Ju
    Department of Biochemistry, Emory University School of Medicine, Rollins Research Center, Atlanta, Georgia, USA
    Methods Enzymol 479:107-22. 2010
    ..The methods described here allow the functional activities of such mutated Cosmc (mCosmc) to be measured and compared to wild-type (wtCosmc)...
  18. ncbi Galectin-1 induces reversible phosphatidylserine exposure at the plasma membrane
    Sean R Stowell
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Mol Biol Cell 20:1408-18. 2009
    ..These results demonstrate that Gal-1-induced PS exposure and subsequent phagocytic removal of living cells represents a new paradigm in cellular turnover...
  19. ncbi Defective angiogenesis and fatal embryonic hemorrhage in mice lacking core 1-derived O-glycans
    Lijun Xia
    Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, 825 N E 13th St, Oklahoma City, OK 73104, USA
    J Cell Biol 164:451-9. 2004
    ..These data reveal an unexpected requirement for core 1-derived O-glycans during angiogenesis...
  20. ncbi Increased susceptibility to colitis and colorectal tumors in mice lacking core 3-derived O-glycans
    Guangyu An
    Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    J Exp Med 204:1417-29. 2007
    ..Moreover, these mice were highly susceptible to experimental triggers of colitis and colorectal adenocarcinoma. These data reveal a requirement for core 3-derived O-glycans in resistance to colonic disease...

Research Grants3

  1. Genetic Basis of Non-Familial IgA Nephropathy
    Tongzhong Ju; Fiscal Year: 2009
    ....
  2. Genetic Basis of Non-Familial IgA Nephropathy
    Tongzhong Ju; Fiscal Year: 2010
    ....