Genomes and Genes
Jason M Hansen
Affiliation: Emory University
- Oxidative stress as a mechanism of teratogenesisJason M Hansen
Department of Pediatrics, Emory School of Medicine, Emory University, Atlanta, Georgia 30322, USA
Birth Defects Res C Embryo Today 78:293-307. 2006..Due to the ability of many known and suspected teratogens to induce oxidative stress and the many signaling pathways that have redox-sensitive components, further research is warranted to fully understand these mechanisms...
- Commensal bacteria modulate cullin-dependent signaling via generation of reactive oxygen speciesAmrita Kumar
Epithelial Pathobiology Unit, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA
EMBO J 26:4457-66. 2007....
- Reactive aldehyde modification of thioredoxin-1 activates early steps of inflammation and cell adhesionYoung Mi Go
Department of Medicine Division of Pulmonary Medicine, Emory University, Atlanta, GA 30322, USA
Am J Pathol 171:1670-81. 2007..Chemical modification of Trx1 by common environmental and endogenously generated reactive aldehydes can contribute to atherosclerosis development by interfering with antioxidant and redox signaling functions of Trx1...
- Redox control of teratogenesisJason M Hansen
Division of Pulmonary, Allergy Immunology, Cystic Fibrosis and Sleep, Department of Pediatrics, Emory School of Medicine, Emory University, Atlanta, GA 30322, United States
Reprod Toxicol 35:165-79. 2013....
- In vivo models of developmental toxicologyJason M Hansen
Department of Pediatrics, Emory University, Atlanta, GA, USA
Methods Mol Biol 889:7-13. 2012..This chapter is dedicated to reviewing the advantages of specific animal models and how these animal models may be used to assess toxicity in the embryo, both descriptively and mechanistically...
- Thioredoxin redox status assessment during embryonic development: the redox WesternJason M Hansen
Department of Pediatrics, Emory University, Atlanta, GA, USA
Methods Mol Biol 889:305-13. 2012..These approaches provide a means to evaluate this unique redox couple during development and allow for the measurement of compartmentalized (mitochondrial vs. cytosolic) oxidation during chemical insult...
- Nuclear and cytoplasmic peroxiredoxin-1 differentially regulate NF-kappaB activitiesJason M Hansen
Department of Pediatrics, Emory School of Medicine, Emory University, 2015 Uppergate Drive 350, Atlanta, GA 30322, USA
Free Radic Biol Med 43:282-8. 2007..Such spatial resolution and protein-specific redox differences imply that the balance of peroxide generation/metabolism in microcompartments provides an important specific component of redox signaling...
- Mitochondrial thioredoxin-2 has a key role in determining tumor necrosis factor-alpha-induced reactive oxygen species generation, NF-kappaB activation, and apoptosisJason M Hansen
Division of Pulmonary, Asthma, Cystic Fibrosis and Sleep, Department of Pediatrics, School of Medicine, Emory University, Atlanta, Georgia 30322, USA
Toxicol Sci 91:643-50. 2006..These findings support the interpretation that mitochondrial-generated ROS is a principal component in TNF-alpha-induced effects and that Trx2 blocks TNF-alpha-induced ROS generation and downstream NF-kappaB activation and apoptosis...
- Nuclear and mitochondrial compartmentation of oxidative stress and redox signalingJason M Hansen
Department of Medicine and Clinical Biomarkers Laboratory, Division of Pulmonary Medicine, Emory University, Atlanta, GA 30322, USA
Annu Rev Pharmacol Toxicol 46:215-34. 2006..The new methods are applicable to a broad range of experimental systems and their use will provide improved understanding of the pharmacologic and toxicologic actions of drugs and toxicants...
- Differential oxidation of thioredoxin-1, thioredoxin-2, and glutathione by metal ionsJason M Hansen
Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, School of Medicine, 615 Michael Street, 225 Whitehead Biomedical Research Building, Emory University, Atlanta, GA 30322, USA
Free Radic Biol Med 40:138-45. 2006....
- Selective oxidative stress in cell nuclei by nuclear-targeted D-amino acid oxidasePatrick J Halvey
Division of Pulmonary, Allergy, Cystic Fibrosis and Sleep, Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, USA
Antioxid Redox Signal 9:807-16. 2007..Furthermore, the attenuation of NF-kappaB reporter activity in the absence of nuclear Trx1 oxidation suggests that critical nuclear redox proteins are subject to control by S-glutathionylation during oxidative stress in the nucleus...
- Selective protection of nuclear thioredoxin-1 and glutathione redox systems against oxidation during glucose and glutamine deficiency in human colonic epithelial cellsYoung Mi Go
Division of Pulmonary Medicine, Emory University, Atlanta, GA 30322, USA
Free Radic Biol Med 42:363-70. 2007..These results further suggest that energy and/or substrate supply may contribute to sensitivity of mitochondrial and cytoplasmic systems to oxidative damage...
- Compartmental oxidation of thiol-disulphide redox couples during epidermal growth factor signallingPatrick J Halvey
Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, School of Medicine, Room 225, Emory University, 615 Michael Street, Atlanta, GA 30322, USA
Biochem J 386:215-9. 2005..Results showed that only cytosolic Trx1 undergoes significant oxidation. Thus EGF signalling involves subcellular compartmental oxidation of Trx1 in the absence of a generalized cellular oxidation...
- Extracellular redox status regulates Nrf2 activation through mitochondrial reactive oxygen speciesBarry R Imhoff
Division of Pulmonary, Allergy Immunology, Cystic Fibrosis and Sleep, Department of Pediatrics, Emory School of Medicine, Emory University, Atlanta, GA 30322, USA
Biochem J 424:491-500. 2009....
- Lactobacillus rhamnosus blocks inflammatory signaling in vivo via reactive oxygen species generationPatricia W Lin
Division of Neonatal Perinatal Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
Free Radic Biol Med 47:1205-11. 2009....
- Extracellular redox environments regulate adipocyte differentiationBarry R Imhoff
Division of Pulmonary, Allergy Immunology, Cystic Fibrosis and Sleep, Emory Department of Pediatrics, Emory University, Atlanta, GA 30322, USA
Differentiation 80:31-9. 2010..Data presented here show that adipocytes/excessive adipose preferentially alter extracellular E(h) to a more oxidized state in vivo and in vitro and may promote further adipogenesis...
- Prohibitin is a novel regulator of antioxidant response that attenuates colonic inflammation in miceArianne L Theiss
Division of Digestive Diseases, Emory University, Atlanta, Georgia 30322, USA
Gastroenterology 137:199-208, 208.e1-6. 2009....
- Maneb and paraquat-mediated neurotoxicity: involvement of peroxiredoxin/thioredoxin systemJAMES R ROEDE
Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, Emory University, Atlanta, Georgia 30322, USA
Toxicol Sci 121:368-75. 2011....
- Tert-butylhydroquinone induces mitochondrial oxidative stress causing Nrf2 activationBarry R Imhoff
Division of Pulmonary, Allergy and Immunology, Cystic Fibrosis and Sleep, Department of Pediatrics, Emory School of Medicine, Emory University, 2015 Uppergate Drive 350, Atlanta, GA 30322, USA
Cell Biol Toxicol 26:541-51. 2010..These data show a compartment-specific effect where tBHQ-induced Nrf2 signaling is mediated by Trx2 and suggest that antioxidant status in various compartments would provide different levels of control of redox signaling...
- Differential redox potential profiles during adipogenesis and osteogenesisBarry R Imhoff
Division of Pulmonary, Allergy Immunology, Cystic Fibrosis and Sleep, Department of Pediatrics, Emory School of Medicine, Emory University Atlanta, 2015 Uppergate Drive Suite 350, Altanta, GA 30322, USA
Cell Mol Biol Lett 16:149-61. 2011....
- Compartmentation of Nrf-2 redox control: regulation of cytoplasmic activation by glutathione and DNA binding by thioredoxin-1Jason M Hansen
Department of Medicine and Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
Toxicol Sci 82:308-17. 2004..In contrast, the ARE reporter was principally controlled by nuclear-targeted Trx-1 and not by GSH. The data show that the GSH and TRX systems have unique, compartmented functions in the control of transcriptional regulation by Nrf-2/ARE...
- A reducing redox environment promotes C2C12 myogenesis: implications for regeneration in aged muscleJason M Hansen
Department of Anesthesiology, School of Medicine, Emory University, 1462 Clifton Road NE, Suite 420, Atlanta, GA 30322, USA
Cell Biol Int 31:546-53. 2007..Thus, oxidative intracellular environments impair myoblast differentiation, while reducing environments favor myogenesis...
- Identification of thioredoxin-2 as a regulator of the mitochondrial permeability transitionMin He
Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Emory University, Atlanta, GA 30322, USA
Toxicol Sci 105:44-50. 2008..Unexpectedly, Trx2 also protected against the MPT induced by Ca(2+) in the absence of added peroxide. The results indicate that in addition to protecting against oxidative stress, Trx2 is an endogenous regulator of the MPT...
- Divergent mechanisms of paraquat, MPP+, and rotenone toxicity: oxidation of thioredoxin and caspase-3 activationSampath Ramachandiran
Center for Neurodegenerative Disease, School of Medicine, Emory University, Atlanta, Georgia 30322, USA
Toxicol Sci 95:163-71. 2007..In addition, intracellular dopamine does not seem to exacerbate the toxicity of these dopaminergic neurotoxicants in this model...
- Loss of total and visceral adipose tissue mass predicts decreases in oxidative stress after weight-loss surgeryNana Gletsu-Miller
Department of Surgery, Emory University School of Medicine, Atlanta, Georgia, USA
Obesity (Silver Spring) 17:439-46. 2009..38 and -0.42). This study found longitudinal associations between general, and more specifically intra-abdominal adiposity, and systemic lipid peroxides, suggesting that adipose tissue mass contributes to oxidative stress...
- Spatial activities and induction of glutamate-cysteine ligase (GCL) in the postimplantation rat embryo and visceral yolk sacJason M Hansen
Department of Biochemistry, Emory University, Atlanta, Georgia 30322, USA
Toxicol Sci 81:371-8. 2004..Following treatments, a marked increase was induced in embryonic GCL(C) content with both DEM (85%) and diamide (19%), but in the VYS, only DEM caused an increase in GCL(C) protein (38%)...
- Disruption of endothelial peroxisome proliferator-activated receptor-gamma reduces vascular nitric oxide productionJennifer M Kleinhenz
Department of Medicine, Atlanta Veterans Affairs and Emory University Medical Centers, Atlanta, Georgia, USA
Am J Physiol Heart Circ Physiol 297:H1647-54. 2009..These findings demonstrate that endothelial PPARgamma regulates vascular nitric oxide production and that the disruption of endothelial PPARgamma contributes to endothelial dysfunction in vivo...
- Methanol, formaldehyde, and sodium formate exposure in rat and mouse conceptuses: a potential role of the visceral yolk sac in embryotoxicityJason M Hansen
Department of Biochemistry, School of Medicine, Emory University, Atlanta, Georgia, USA
Birth Defects Res A Clin Mol Teratol 73:72-82. 2005..Methanol (CH3OH) is believed to be teratogenic based on rodent studies. The mouse is more sensitive than the rat, but mechanisms of toxicity and identification of teratogenic metabolites are uncertain...
- Retinoic acid stimulation of VEGF secretion from human endometrial stromal cells is mediated by production of reactive oxygen speciesJuanjuan Wu
Department of Gynecology and Obstetrics, Emory University School of Medicine, 1639 Pierce Drive, Atlanta, GA 30322, USA
J Physiol 589:863-75. 2011..Together, these findings predict a redox-mediated mechanism for retinoid regulation of localized VEGF secretion in the human endometrium that may be necessary for the successful establishment of pregnancy...
- The role of mitochondrial oxidation in endotoxin-induced liver-dependent swine pulmonary edemaAmsel M Siore
Center for Translational Research in the Lungs, McKelvey Center for Lung Transplantation, Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine and Predictive Health Institute, Emory University School of Medicine, Atlanta, GA 30322, USA
Pulm Pharmacol Ther 25:407-12. 2012..Procysteine reduced thioredoxin-2 oxidation, attenuated hemodynamic, gas exchange, hepatocellular dysfunction, and cytokine responses and prevented lung edema. n-acetylcysteine had more modest effects and did not prevent lung edema...
- SOD1 targeted to the mitochondrial intermembrane space prevents motor neuropathy in the Sod1 knockout mouseLindsey R Fischer
Department of Neurology, Emory University School of Medicine, Atlanta, GA 30322, USA
Brain 134:196-209. 2011..These results suggest that SOD1 in the mitochondrial intermembrane space is fundamental for motor axon maintenance, and implicate oxidative damage initiated at mitochondrial sites in the pathogenesis of motor axon degeneration...
- Thalidomide modulates nuclear redox status and preferentially depletes glutathione in rabbit limb versus rat limbJason M Hansen
University of Michigan, Department of Environmental Health Sciences Toxicology Program, Ann Arbor, Michigan, USA
J Pharmacol Exp Ther 300:768-76. 2002..Redox shifts in the nucleus may result in the misregulation of transcription factor/DNA interactions and cause defective growth and development...
- A novel hypothesis for thalidomide-induced limb teratogenesis: redox misregulation of the NF-kappaB pathwayJason M Hansen
Antioxid Redox Signal 6:1-14. 2004..We hypothesize that species-selective alterations in redox microenvironment caused by free radical production from thalidomide results in attenuation of the NF-kappaB-mediated gene expression that is responsible for limb outgrowth...
- Misregulation of gene expression in the redox-sensitive NF-kappab-dependent limb outgrowth pathway by thalidomideJason M Hansen
Toxicology Program, Environmental Health Sciences, School of Public Health, University of Michigan, Ann Arbor, Michigan 48109, USA
Dev Dyn 225:186-94. 2002..These findings show that NF-kappaB binding is diminished due to selective thalidomide-induced redox changes in the rabbit, resulting in the significant attenuation of expression of genes necessary for limb outgrowth...
- Effect of mercury(II) on Nrf2, thioredoxin reductase-1 and thioredoxin-1 in human monocytesJohn C Wataha
University of Washington, Seattle, WA 98195 7456, USA
Dent Mater 24:765-72. 2008..In the current study, we tested a hypothesis that concentrations of mercury <100nM altered levels and activities of key proteins that maintain monocytic redox balance...
- Redox control of Nrf2/ARE by thioredoxin and GSHJason Hansen; Fiscal Year: 2005..The roles of GSH and cytosolic and nuclear TRX during NRF2 activation and binding will provide an essential foundation for future developmental toxicology. ..