Paraskevi Giannakakou

Summary

Affiliation: Emory University School of Medicine
Country: USA

Publications

  1. pmc Enhanced microtubule-dependent trafficking and p53 nuclear accumulation by suppression of microtubule dynamics
    Paraskevi Giannakakou
    Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30322, USA
    Proc Natl Acad Sci U S A 99:10855-60. 2002
  2. pmc A folate receptor-targeting nanoparticle minimizes drug resistance in a human cancer model
    Xu Wang
    Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, Georgia, USA
    ACS Nano 5:6184-94. 2011
  3. pmc EF24, a novel curcumin analog, disrupts the microtubule cytoskeleton and inhibits HIF-1
    Shala L Thomas
    Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia, USA
    Cell Cycle 7:2409-17. 2008
  4. pmc The protein farnesyltransferase regulates HDAC6 activity in a microtubule-dependent manner
    Jun Zhou
    Winship Cancer Institute, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 284:9648-55. 2009
  5. pmc Resistance to microtubule-stabilizing drugs involves two events: beta-tubulin mutation in one allele followed by loss of the second allele
    Yuefang Wang
    Winship Cancer Institute, Emory University School of Medicine, Atlanta, Georgia, USA
    Cell Cycle 4:1847-53. 2005
  6. pmc Farnesyltransferase inhibitors reverse taxane resistance
    Adam I Marcus
    Winship Cancer Institute, Emory University, Atlanta, Georgia, USA
    Cancer Res 66:8838-46. 2006
  7. ncbi request reprint Farnesyl transferase inhibitors impair chromosomal maintenance in cell lines and human tumors by compromising CENP-E and CENP-F function
    Katherine Schafer-Hales
    Winship Cancer Institute, Emory University, Atlanta, GA 30322, USA
    Mol Cancer Ther 6:1317-28. 2007
  8. pmc The synergistic combination of the farnesyl transferase inhibitor lonafarnib and paclitaxel enhances tubulin acetylation and requires a functional tubulin deacetylase
    Adam I Marcus
    Winship Cancer Institute, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Cancer Res 65:3883-93. 2005
  9. ncbi request reprint Targeting microtubules for cancer chemotherapy
    Jun Zhou
    Department of Cell Biology, Emory University School of Medicine, 615 Michael Street, Room 455, Atlanta, GA 30322, USA
    Curr Med Chem Anticancer Agents 5:65-71. 2005
  10. ncbi request reprint Both microtubule-stabilizing and microtubule-destabilizing drugs inhibit hypoxia-inducible factor-1alpha accumulation and activity by disrupting microtubule function
    Daniel Escuin
    Department of Hematology and Oncology, Winship Cancer Institute, Robert Woodruff Health Sciences Center, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Cancer Res 65:9021-8. 2005

Collaborators

Detail Information

Publications33

  1. pmc Enhanced microtubule-dependent trafficking and p53 nuclear accumulation by suppression of microtubule dynamics
    Paraskevi Giannakakou
    Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30322, USA
    Proc Natl Acad Sci U S A 99:10855-60. 2002
    ..We propose that suppression of MT dynamics by low concentrations of MTCs enhances MT-dependent trafficking toward the minus ends of MTs and facilitates nuclear targeting...
  2. pmc A folate receptor-targeting nanoparticle minimizes drug resistance in a human cancer model
    Xu Wang
    Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, Georgia, USA
    ACS Nano 5:6184-94. 2011
    ..These results indicated that the targeted HFT-T nanoparticle may be promising in minimizing P-gp related drug resistance and enhancing therapeutic efficacy compared with the free form of paclitaxel...
  3. pmc EF24, a novel curcumin analog, disrupts the microtubule cytoskeleton and inhibits HIF-1
    Shala L Thomas
    Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia, USA
    Cell Cycle 7:2409-17. 2008
    ....
  4. pmc The protein farnesyltransferase regulates HDAC6 activity in a microtubule-dependent manner
    Jun Zhou
    Winship Cancer Institute, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 284:9648-55. 2009
    ..Altogether, these data suggest that FTase, via its tubulin-association, is a critical upstream regulator of HDAC6 activity and that FTase expression could help stratify cancer patients that would most benefit from this treatment...
  5. pmc Resistance to microtubule-stabilizing drugs involves two events: beta-tubulin mutation in one allele followed by loss of the second allele
    Yuefang Wang
    Winship Cancer Institute, Emory University School of Medicine, Atlanta, Georgia, USA
    Cell Cycle 4:1847-53. 2005
    ..Acquired tubulin mutations in conjunction with LOH for the wt tubulin resulted in a highly resistant phenotype, revealing a new mechanism for taxane resistance...
  6. pmc Farnesyltransferase inhibitors reverse taxane resistance
    Adam I Marcus
    Winship Cancer Institute, Emory University, Atlanta, Georgia, USA
    Cancer Res 66:8838-46. 2006
    ....
  7. ncbi request reprint Farnesyl transferase inhibitors impair chromosomal maintenance in cell lines and human tumors by compromising CENP-E and CENP-F function
    Katherine Schafer-Hales
    Winship Cancer Institute, Emory University, Atlanta, GA 30322, USA
    Mol Cancer Ther 6:1317-28. 2007
    ..This analysis revealed three hydrophobic patches on the tubulin dimer for insertion of a farnesyl group, alluding to the possibility of an association between a farnesyl group and the microtubule...
  8. pmc The synergistic combination of the farnesyl transferase inhibitor lonafarnib and paclitaxel enhances tubulin acetylation and requires a functional tubulin deacetylase
    Adam I Marcus
    Winship Cancer Institute, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Cancer Res 65:3883-93. 2005
    ..Taken together, these data suggest a relationship between FT inhibition, HDAC6 function, and cell death, providing insight into the putative molecular basis of the lonafarnib/taxane synergistic antiproliferative combination...
  9. ncbi request reprint Targeting microtubules for cancer chemotherapy
    Jun Zhou
    Department of Cell Biology, Emory University School of Medicine, 615 Michael Street, Room 455, Atlanta, GA 30322, USA
    Curr Med Chem Anticancer Agents 5:65-71. 2005
    ....
  10. ncbi request reprint Both microtubule-stabilizing and microtubule-destabilizing drugs inhibit hypoxia-inducible factor-1alpha accumulation and activity by disrupting microtubule function
    Daniel Escuin
    Department of Hematology and Oncology, Winship Cancer Institute, Robert Woodruff Health Sciences Center, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Cancer Res 65:9021-8. 2005
    ..These data directly link beta-tubulin drug binding with HIF-1alpha protein inhibition. Our results further provide a strong rationale for testing taxanes and epothilones in clinical trials targeting HIF-1 in cancer patients...
  11. ncbi request reprint Automatic microtubule tracking for QD-based in vivo cell imaging and drug efficacy study
    Koon Yin Kong
    Sch of Electr and Comput Eng, Georgia Inst of Technol, Atlanta, GA 30332, USA
    Conf Proc IEEE Eng Med Biol Soc 1:3321-4. 2006
    ..This algorithm has been validated using simulated images, images of untreated MCF-7 breast cancer cells, and image of cells treated with the microtubule-targeting chemotherapeutic agent, Taxol...
  12. pmc HFT-T, a targeting nanoparticle, enhances specific delivery of paclitaxel to folate receptor-positive tumors
    Xu Wang
    Department of Hematology and Medical Oncology, Winship Cancer Institute, Atlanta, Georgia, USA
    ACS Nano 3:3165-74. 2009
    ..These results indicate that specific delivery of paclitaxel with a ternary structured nanoparticle (HFT-T) targeting FR-positive tumor is a promising strategy to enhance chemotherapy efficacy and minimize adverse effects...
  13. ncbi request reprint Survivin deregulation in beta-tubulin mutant ovarian cancer cells underlies their compromised mitotic response to taxol
    Jun Zhou
    Winship Cancer Institute and Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Cancer Res 64:8708-14. 2004
    ..Our results show the importance of survivin for the mitotic response in the context of Taxol resistance and provide novel insights into the mechanisms of mitotic arrest and apoptosis induced by microtubule-targeting agents...
  14. pmc Mitotic kinesin inhibitors induce mitotic arrest and cell death in Taxol-resistant and -sensitive cancer cells
    Adam I Marcus
    Winship Cancer Institute, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 280:11569-77. 2005
    ..Taken together, our results show that Eg5 inhibitors have promising anticancer activity and can be potentially used to overcome Taxol resistance in the clinical setting...
  15. ncbi request reprint Identification of a novel small-molecule inhibitor of the hypoxia-inducible factor 1 pathway
    Chalet Tan
    Department of Neurosurgery, Emory University School of Medicine, Atlanta, GA 30322, USA
    Cancer Res 65:605-12. 2005
    ..Further studies on the mechanism of action of 103D5R will likely provide new insights into its validity/applicability for the pharmacologic targeting of HIF-1alpha for therapeutic purposes...
  16. pmc Syntheses and biological activities of novel 2-methoxyestradiol analogs, 2-fluoroethoxyestradiol and 2-fluoropropanoxyestradiol, and a radiosynthesis of 2-[(18)F]fluoroethoxyestradiol for positron emission tomography
    JiYoung Mun
    Department of Radiology, Emory University, Atlanta, GA 30322, USA
    Nucl Med Biol 35:615-22. 2008
    ....
  17. ncbi request reprint Antitumor effect of 2-methoxyestradiol in a rat orthotopic brain tumor model
    Seung Hee Kang
    Department of Hematology Oncology, Emory University, School of Medicine, Atlanta, Georgia 30322, USA
    Cancer Res 66:11991-7. 2006
    ....
  18. pmc Farnesyl transferase expression determines clinical response to the docetaxel-lonafarnib combination in patients with advanced malignancies
    John Kauh
    Department of Hematology and Medical Oncology, Emory University School of Medicine and Winship Cancer Institute, Atlanta, Georgia, USA
    Cancer 117:4049-59. 2011
    ..This pilot study evaluated the biological effects of LNF and docetaxel (DTX) combination therapy in refractory solid tumors by comparing pretreatment and post-treatment tumor biopsies...
  19. ncbi request reprint Exploitation of the HIF axis for cancer therapy
    Daniel Escuin
    Winship Cancer Institute, Emory University, Atlanta, Georgia 30322, USA
    Cancer Biol Ther 3:608-11. 2004
    ..We will overview inhibitors of HIF-1alpha and will discuss their potential use for cancer therapy...
  20. doi request reprint Integrating image analysis algorithms in a web interface for the quantification of microtubule dynamics
    Koon Yin Kong
    School of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
    Int J Comput Biol Drug Des 5:298-313. 2012
    ..We also demonstrate how to re-evaluate existing data in a case study using real imaging data. Lastly, we show the added functionalities of the interface including how to share image data and analysis results...
  21. ncbi request reprint Paclitaxel-resistant human ovarian cancer cells undergo c-Jun NH2-terminal kinase-mediated apoptosis in response to noscapine
    Jun Zhou
    Graduate Program in Biochemistry, Cell and Developmental Biology, Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 277:39777-85. 2002
    ..In addition, our results suggest that the JNK pathway plays an essential role in microtubule inhibitor-induced apoptosis...
  22. ncbi request reprint 2ME2 inhibits tumor growth and angiogenesis by disrupting microtubules and dysregulating HIF
    Nicola J Mabjeesh
    Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30322, USA
    Cancer Cell 3:363-75. 2003
    ..These data establish 2ME2 as a small molecule inhibitor of HIF-1 and provide a mechanistic link between the disruption of the MT cytoskeleton and inhibition of angiogenesis...
  23. ncbi request reprint The importance of p53 location: nuclear or cytoplasmic zip code?
    Aurora O'Brate
    Winship Cancer Institute, Emory University, 1365 C Clifton Road, N E, Room C4078, Atlanta, GA 30322, USA
    Drug Resist Updat 6:313-22. 2003
    ..Finally, a portion of p53 is localized to the mitochondria as part of the non-transcriptional apoptotic response. In this review we strive to present the most recent data on how the activity of p53 is regulated by its location...
  24. ncbi request reprint Microtubule interactions with chemically diverse stabilizing agents: thermodynamics of binding to the paclitaxel site predicts cytotoxicity
    Ruben M Buey
    Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Cientificas, Ramiro de Maeztu 9, 28040 Madrid, Spain
    Chem Biol 12:1269-79. 2005
    ....
  25. ncbi request reprint Interaction of epothilone analogs with the paclitaxel binding site: relationship between binding affinity, microtubule stabilization, and cytotoxicity
    Ruben M Buey
    Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Cientificas, Ramiro de Maeztu 9, 28040 Madrid, Spain
    Chem Biol 11:225-36. 2004
    ..The binding constants correlate well with IC(50) values, demonstrating that affinity measurements are a useful tool for drug design...
  26. ncbi request reprint Cyclostreptin (FR182877), an antitumor tubulin-polymerizing agent deficient in enhancing tubulin assembly despite its high affinity for the taxoid site
    Michael C Edler
    Screening Technologies Branch, Developmental Therapeutics Program, Division of Cancer Treatment and Diagnosis, National Cancer Institute at Frederick, National Institutes of Health, Frederick, Maryland 21702, USA
    Biochemistry 44:11525-38. 2005
    ..Initial studies also indicate that, relative to paclitaxel, cyclostreptin is more deficient in nucleation than elongation of polymer...
  27. pmc Evaluation of the tubulin-bound paclitaxel conformation: synthesis, biology, and SAR studies of C-4 to C-3' bridged paclitaxel analogues
    Thota Ganesh
    Department of Chemistry, M C 0212, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA
    J Med Chem 50:713-25. 2007
    ..In this study we report additional experimental data and calculations that delineate the allowable parameters for effective paclitaxel-tubulin interactions...
  28. ncbi request reprint Total synthesis and biological evaluation of (-)apicularen A and analogues thereof
    K C Nicolaou
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Chemistry 9:6177-91. 2003
    ....
  29. ncbi request reprint Total synthesis of apoptolidin: completion of the synthesis and analogue synthesis and evaluation
    K C Nicolaou
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    J Am Chem Soc 125:15443-54. 2003
    ..The developed synthetic technology was applied to the construction of several analogues including 74, 75, and 77 which exhibit significant bioactivity against tumor cells...
  30. ncbi request reprint Design, synthesis, and biological properties of highly potent epothilone B analogues
    K C Nicolaou
    Department of Chemistry and, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Angew Chem Int Ed Engl 42:3515-20. 2003
  31. ncbi request reprint beta-Tubulin mutations are associated with resistance to 2-methoxyestradiol in MDA-MB-435 cancer cells
    Yesim Gokmen-Polar
    Department of Medicine, Indiana University School of Medicine, and Indiana Centers for Applied Protein Sciences, Indianapolis, IN 46202 5254, USA
    Cancer Res 65:9406-14. 2005
    ....
  32. ncbi request reprint Molecular design and chemical synthesis of a highly potent epothilone
    K C Nicolaou
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    ChemMedChem 1:41-4. 2006
  33. ncbi request reprint The microtubule stabilizing agent laulimalide does not bind in the taxoid site, kills cells resistant to paclitaxel and epothilones, and may not require its epoxide moiety for activity
    Donald E Pryor
    Screening Technologies Branch, Developmental Therapeutics Program, Division of Cancer Treatment and Diagnosis, National Cancer Institute at Frederick, National Institutes of Health, Frederick, Maryland 21702, USA
    Biochemistry 41:9109-15. 2002
    ..Further exploration of the role of the epoxide in the interaction of laulimalide with tubulin is therefore justified...