James A McCubrey

Summary

Affiliation: East Carolina University
Country: USA

Publications

  1. ncbi request reprint Docetaxel cytotoxicity is enhanced by inhibition of the Raf/MEK/ERK signal transduction pathway
    Patrick M Navolanic
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, North Carolina 27858, USA
    Cancer Biol Ther 2:677-8. 2003
  2. ncbi request reprint Ability of the activated PI3K/Akt oncoproteins to synergize with MEK1 and induce cell cycle progression and abrogate the cytokine-dependence of hematopoietic cells
    John G Shelton
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina 27858, USA
    Cell Cycle 3:503-12. 2004
  3. ncbi request reprint Upping the bid on engineered inducible cell death
    James A McCubrey
    Department of Microbiology and Immunology, Leo Jenkins Cancer Center, Brody School of Medicine at East Carolina University, Greenville, NC 27858, USA
    Cancer Biol Ther 4:537. 2005
  4. ncbi request reprint Effects of a conditionally active v-ErbB and an EGF-R inhibitor on transformation of NIH-3T3 cells and abrogation of cytokine dependency of hematopoietic cells
    James A McCubrey
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC 27858, USA
    Oncogene 23:7810-20. 2004
  5. pmc Enhancing therapeutic efficacy by targeting non-oncogene addicted cells with combinations of signal transduction inhibitors and chemotherapy
    Stephen L Abrams
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC, USA
    Cell Cycle 9:1839-46. 2010
  6. pmc The Raf/MEK/ERK pathway can govern drug resistance, apoptosis and sensitivity to targeted therapy
    Stephen L Abrams
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC, USA
    Cell Cycle 9:1781-91. 2010
  7. pmc Ectopic NGAL expression can alter sensitivity of breast cancer cells to EGFR, Bcl-2, CaM-K inhibitors and the plant natural product berberine
    William H Chappell
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC USA
    Cell Cycle 11:4447-61. 2012
  8. pmc Involvement of Akt and mTOR in chemotherapeutic- and hormonal-based drug resistance and response to radiation in breast cancer cells
    Linda S Steelman
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC, USA
    Cell Cycle 10:3003-15. 2011
  9. ncbi request reprint Molecular pathways leading to oxidative stress-induced phosphorylation of Akt
    Michelle M Lahair
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, North Carolina 27834, USA
    Antioxid Redox Signal 8:1749-56. 2006
  10. doi request reprint Emerging MEK inhibitors
    James A McCubrey
    Brody School of Medicine at East Carolina University, Department of Microbiology and Immunology, 600 Moye Boulevard, Greenville, NC 27858, USA
    Expert Opin Emerg Drugs 15:203-23. 2010

Collaborators

Detail Information

Publications89

  1. ncbi request reprint Docetaxel cytotoxicity is enhanced by inhibition of the Raf/MEK/ERK signal transduction pathway
    Patrick M Navolanic
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, North Carolina 27858, USA
    Cancer Biol Ther 2:677-8. 2003
  2. ncbi request reprint Ability of the activated PI3K/Akt oncoproteins to synergize with MEK1 and induce cell cycle progression and abrogate the cytokine-dependence of hematopoietic cells
    John G Shelton
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina 27858, USA
    Cell Cycle 3:503-12. 2004
    ..Likewise, suppression of multiple signal transduction pathways is a more effective means to inhibit cell cycle progression and induce apoptosis in leukemic cells...
  3. ncbi request reprint Upping the bid on engineered inducible cell death
    James A McCubrey
    Department of Microbiology and Immunology, Leo Jenkins Cancer Center, Brody School of Medicine at East Carolina University, Greenville, NC 27858, USA
    Cancer Biol Ther 4:537. 2005
  4. ncbi request reprint Effects of a conditionally active v-ErbB and an EGF-R inhibitor on transformation of NIH-3T3 cells and abrogation of cytokine dependency of hematopoietic cells
    James A McCubrey
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC 27858, USA
    Oncogene 23:7810-20. 2004
    ....
  5. pmc Enhancing therapeutic efficacy by targeting non-oncogene addicted cells with combinations of signal transduction inhibitors and chemotherapy
    Stephen L Abrams
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC, USA
    Cell Cycle 9:1839-46. 2010
    ..These results demonstrate the enhanced effectiveness of targeting early hematopoietic progenitor cells with combinations of chemotherapeutic drugs and signal transduction inhibitors...
  6. pmc The Raf/MEK/ERK pathway can govern drug resistance, apoptosis and sensitivity to targeted therapy
    Stephen L Abrams
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC, USA
    Cell Cycle 9:1781-91. 2010
    ..These results indicate that targeting the Raf/MEK/ERK and PI3K/Akt/mTOR pathways may be an effective approach for therapeutic intervention in drug resistant cancers that have mutations activating these cascades...
  7. pmc Ectopic NGAL expression can alter sensitivity of breast cancer cells to EGFR, Bcl-2, CaM-K inhibitors and the plant natural product berberine
    William H Chappell
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC USA
    Cell Cycle 11:4447-61. 2012
    ....
  8. pmc Involvement of Akt and mTOR in chemotherapeutic- and hormonal-based drug resistance and response to radiation in breast cancer cells
    Linda S Steelman
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC, USA
    Cell Cycle 10:3003-15. 2011
    ..Understanding how breast cancers respond to chemo- and hormonal-based therapies and radiation may enhance the ability to treat breast cancer more effectively...
  9. ncbi request reprint Molecular pathways leading to oxidative stress-induced phosphorylation of Akt
    Michelle M Lahair
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, North Carolina 27834, USA
    Antioxid Redox Signal 8:1749-56. 2006
    ..In this pathway, neither the Src kinases nor PI3K are required. The other pathway mediated by hydrogen peroxide results in the phosphorylation of Akt on S473 and requires CaM-K, PI3K, and Src activity...
  10. doi request reprint Emerging MEK inhibitors
    James A McCubrey
    Brody School of Medicine at East Carolina University, Department of Microbiology and Immunology, 600 Moye Boulevard, Greenville, NC 27858, USA
    Expert Opin Emerg Drugs 15:203-23. 2010
    ..This review will cover the scientific literature from 1980 to present and is a follow on from a review which focused on Emerging Raf Inhibitors published in this same review series...
  11. ncbi request reprint Roles of the RAF/MEK/ERK and PI3K/PTEN/AKT pathways in malignant transformation and drug resistance
    James A McCubrey
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC 27858, USA
    Adv Enzyme Regul 46:249-79. 2006
    ..These signaling and anti-apoptotic pathways can have different effects on growth, prevention of apoptosis and induction of drug resistance in cells of various lineages which may be due to the expression of lineage-specific factors...
  12. pmc Targeting the RAF/MEK/ERK, PI3K/AKT and p53 pathways in hematopoietic drug resistance
    James A McCubrey
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University Greenville, NC 27858, USA
    Adv Enzyme Regul 47:64-103. 2007
  13. ncbi request reprint Conditional EGFR promotes cell cycle progression and prevention of apoptosis in the absence of autocrine cytokines
    John G Shelton
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina 27858, USA
    Cell Cycle 4:822-30. 2005
    ..Thus this conditional ErbB construct is useful to elucidate EGFR signaling and anti-apoptotic pathways in the absence of autocrine cytokine expression...
  14. pmc Roles of the Raf/MEK/ERK pathway in cell growth, malignant transformation and drug resistance
    James A McCubrey
    Department of Microbiology and Immunology, Leo Jenkins Cancer Center, Brody School of Medicine at East Carolina University, Greenville, NC 27858, USA
    Biochim Biophys Acta 1773:1263-84. 2007
    ....
  15. doi request reprint Emerging Raf inhibitors
    James A McCubrey
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC 27858, USA
    Expert Opin Emerg Drugs 14:633-48. 2009
    ..The Raf/MAPK kinase/extracellular-signal-regulated kinase pathway has profound effects on proliferative, apoptotic and differentiation pathways as well as the sensitivity and resistance to chemotherapeutic drugs...
  16. pmc Dominant roles of the Raf/MEK/ERK pathway in cell cycle progression, prevention of apoptosis and sensitivity to chemotherapeutic drugs
    Linda S Steelman
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC, USA
    Cell Cycle 9:1629-38. 2010
    ..Targeting the Raf/MEK/ERK and PI3K/PTEN/Akt/mTOR pathways may be an effective approach for therapeutic intervention in those cancers which have upstream mutations which result in activation of these pathways...
  17. ncbi request reprint EGF induces cell motility and multi-drug resistance gene expression in breast cancer cells
    Ruben Garcia
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, North Carolina 27858, USA
    Cell Cycle 5:2820-6. 2006
    ..In conclusion, treatment of MCF-7 breast cancer cells with EGF, in the absence of other growth factors, resulted in activation of EGFR signal transduction pathways; which were related with cell motility and drug resistance...
  18. ncbi request reprint Raf promotes human herpesvirus-8 (HHV-8/KSHV) infection
    Shaw M Akula
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27858, USA
    Oncogene 23:5227-41. 2004
    ..Here we report Raf expression to significantly enhance HHV-8 infection of target cells. These findings implicate a role for Raf not only in the infectious entry of HHV-8 but also in modulating KS pathogenesis...
  19. ncbi request reprint Inhibition of CREB transcriptional activity in human T lymphocytes by oxidative stress
    Oswaldo G Rodriguez-Mora
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC 27834, USA
    Free Radic Biol Med 38:1653-61. 2005
    ..These data provide insight into a potential mechanism by which oxidative stress can alter T cell receptor-induced CREB activation and responsiveness...
  20. ncbi request reprint Inhibition of the CaM-kinases augments cell death in response to oxygen radicals and oxygen radical inducing cancer therapies in MCF-7 human breast cancer cells
    Oswaldo G Rodriguez-Mora
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, North Carolina 27834, USA
    Cancer Biol Ther 5:1022-30. 2006
    ..This is the first time that CaM-K inhibition is reported to sensitize cancer cells to reactive oxygen intermediate inducing cancer treatments...
  21. ncbi request reprint Targeting the PI3K and MAPK pathways to treat Kaposi's-sarcoma-associated herpes virus infection and pathogenesis
    Phelps J Lambert
    Brody School of Medicine at East Carolina University, Department of Microbiology and Immunology, Greenville, NC 27834, USA
    Expert Opin Ther Targets 11:589-99. 2007
    ..Manipulation of the PI3K and MAPK pathways also plays a role in malignant transformation. Here, the authors review the potential to target the PI3K and MAPK signaling pathways to inhibit KSHV infection and pathogenesis...
  22. ncbi request reprint Raf-1 and Bcl-2 induce distinct and common pathways that contribute to breast cancer drug resistance
    Julianne M Davis
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC 27858, USA
    Clin Cancer Res 9:1161-70. 2003
    ..These observations suggest both independent and overlapping roles for Raf-1 and Bcl-2 oncogenes in the resistance to growth inhibition by doxorubicin...
  23. ncbi request reprint Elucidation of signal transduction pathways by transfection of cells with modified oncogenes
    Linda S Steelman
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC, USA
    Methods Mol Biol 218:203-20. 2003
    ..Various strong and weak points about three different techniques of stable gene transfer, including calcium-phosphate DNA precipitation, transfection via liposomes, and transfection via electroporation, will be discussed...
  24. ncbi request reprint B-raf and insulin synergistically prevent apoptosis and induce cell cycle progression in hematopoietic cells
    John G Shelton
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina 27858, USA
    Cell Cycle 3:189-96. 2004
    ..Raf isoforms differ in their abilities to control apoptosis and cell cycle progression and B-Raf requires insulin-activated pathways for full antiapoptotic and proliferative activity...
  25. ncbi request reprint Akt inactivates ERK causing decreased response to chemotherapeutic drugs in advanced CaP cells
    John T Lee
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, North Carolina 27858, USA
    Cell Cycle 7:631-6. 2008
    ..The genotype of the tumor must be considered for an effective response to these and other antineoplastic drugs...
  26. pmc Targeting prostate cancer based on signal transduction and cell cycle pathways
    John T Lee
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, North Carolina 27858, USA
    Cell Cycle 7:1745-62. 2008
    ..g., p53, p21(Cip1), p27(Kip1), Rb, etc.,). This review focuses on the aberrant interactions of signal transduction and cell cycle genes products and how they can contribute to prostate cancer and alter therapeutic effectiveness...
  27. ncbi request reprint Effects of the RAF/MEK/ERK and PI3K/AKT signal transduction pathways on the abrogation of cytokine-dependence and prevention of apoptosis in hematopoietic cells
    John G Shelton
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27858, USA
    Oncogene 22:2478-92. 2003
    ....
  28. pmc Ras/Raf/MEK/ERK and PI3K/PTEN/Akt/mTOR cascade inhibitors: how mutations can result in therapy resistance and how to overcome resistance
    James A McCubrey
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC, USA
    Oncotarget 3:1068-111. 2012
    ....
  29. ncbi request reprint Activated EGFR promotes the survival of B-lineage acute leukemia in the absence of stromal cells
    Justin D Spengeman
    Department of Microbiology and Immunology, The Brody School of Medicine at East Carolina University, Greenville, North Carolina 27834, USA
    Cell Cycle 4:483-7. 2005
    ..These data provide evidence that stimulation of the EGFR pathway is one mechanism by which the bone marrow microenvironment may contribute to the growth and survival of B-cell acute leukemia...
  30. ncbi request reprint Modulation of Raf/MEK/ERK kinase activity does not affect the chemoresistance profile of advanced prostate cancer cells
    John T Lee
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC 27858, USA
    Int J Oncol 26:1637-44. 2005
    ..Together, these data demonstrate that the response of prostatic tumors to the chemotherapeutic compounds doxorubicin and paclitaxel is independent of Raf/MEK/ERK signaling...
  31. pmc Ras/Raf/MEK/ERK and PI3K/PTEN/Akt/mTOR inhibitors: rationale and importance to inhibiting these pathways in human health
    William H Chappell
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, USA
    Oncotarget 2:135-64. 2011
    ..Inhibitors targeting these pathways have many potential uses from suppression of cancer, proliferative diseases as well as aging...
  32. ncbi request reprint Elucidation of signal transduction pathways by retroviral infection of cells with modified oncogenes
    Linda S Steelman
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC, USA
    Methods Mol Biol 218:221-52. 2003
    ....
  33. pmc Mutations and deregulation of Ras/Raf/MEK/ERK and PI3K/PTEN/Akt/mTOR cascades which alter therapy response
    James A McCubrey
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC, USA
    Oncotarget 3:954-87. 2012
    ..This review will first describe these pathways and discuss how genetic mutations and epigenetic alterations can result in resistance to various inhibitors...
  34. pmc Distinct roles for p107 and p130 in Rb-independent cellular senescence
    Brian D Lehmann
    Department of Anatomy and Cell Biology, Brody School of Medicine at East Carolina University, Greenville, North Carolina, USA
    Cell Cycle 7:1262-8. 2008
    ....
  35. ncbi request reprint Two targets are better than one. Promising combination therapy to treat breast cancer
    Richard A Franklin
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, North Carolina 27834, USA
    Cancer Biol Ther 4:1190-1. 2005
  36. doi request reprint Therapeutic resistance resulting from mutations in Raf/MEK/ERK and PI3K/PTEN/Akt/mTOR signaling pathways
    James A McCubrey
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, North Carolina 27858, USA
    J Cell Physiol 226:2762-81. 2011
    ..Controlling the expression of these pathways could improve cancer therapy and ameliorate human health...
  37. ncbi request reprint Effects of endogenous epidermal growth factor receptor signaling on DNA synthesis and ERK activation in a cytokine-dependent hematopoietic cell line
    John G Shelton
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina 27858, USA
    Cell Cycle 4:818-21. 2005
    ..These results document the functional importance of endogenous EGFR signaling pathway in some hematopoietic cells...
  38. ncbi request reprint Phosphatidylinositol 3'-kinase activation leads to multidrug resistance protein-1 expression and subsequent chemoresistance in advanced prostate cancer cells
    John T Lee
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, North Carolina, USA
    Cancer Res 64:8397-404. 2004
    ..Thus, inhibition of PI3K activity with concomitant administration of chemotoxic compounds may prove beneficial in preventing the development of drug resistance in patients with hormone-refractory prostate cancer...
  39. ncbi request reprint Regulation of cell cycle progression and apoptosis by the Ras/Raf/MEK/ERK pathway (Review)
    Fumin Chang
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC 27858, USA
    Int J Oncol 22:469-80. 2003
    ..This review will discuss the regulation of the Ras/Raf/MEK/ERK pathway and how it modulates cell cycle progression and cell survival...
  40. ncbi request reprint Targeting the cancer initiating cell: the ultimate target for cancer therapy
    James A McCubrey
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC 27858, USA
    Curr Pharm Des 18:1784-95. 2012
    ..New approaches should be developed to effectively target the CIC which could vastly improve cancer therapies and outcomes. This review will discuss recent concepts of targeting CICs in certain leukemia models...
  41. ncbi request reprint GSK-3beta is a critical mediator of tetrandrine induced cell cycle arrest and cytotoxicity
    James A McCubrey
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, North Carolina 27858, USA
    Cancer Biol Ther 7:1079. 2008
  42. ncbi request reprint Targeting the Raf/MEK/ERK pathway with small-molecule inhibitors
    James A McCubrey
    Brody School of Medicine at East Carolina University, Department of Microbiology and Immunology, 600 Moye Boulevard, Greenville, NC 27858, USA
    Curr Opin Investig Drugs 9:614-30. 2008
    ..The effective targeting of this pathway may result in the suppression of cell growth, and death of malignant cells. This review focuses on targeting the Raf/MEK/ERK pathway with small-molecule inhibitors for the treatment of cancer...
  43. ncbi request reprint Vascular endothelial growth factor augments human herpesvirus-8 (HHV-8/KSHV) infection
    Patrick W Ford
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina 27834, USA
    Cancer Biol Ther 3:876-81. 2004
    ..These findings suggest that targeting VEGF/VEGFR may prove efficacious in controlling HHV-8 associated pathogenesis...
  44. ncbi request reprint B-Raf-dependent expression of vascular endothelial growth factor-A in Kaposi sarcoma-associated herpesvirus-infected human B cells
    Shaw M Akula
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA
    Blood 105:4516-22. 2005
    ..Taken together, we report for the first time the ability of Raf-associated signaling to play a role in the expression of VEGF-A in KSHV-infected hematopoietic cells...
  45. ncbi request reprint EGFR family signaling and its association with breast cancer development and resistance to chemotherapy (Review)
    Patrick M Navolanic
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27858, USA
    Int J Oncol 22:237-52. 2003
    ..An appreciation of how these signaling pathways relate to development of breast cancer and its response to chemotherapy may lead to improved prevention, diagnosis, and treatment of this disease...
  46. ncbi request reprint Calcium/calmodulin-dependent kinase I and calcium/calmodulin-dependent kinase kinase participate in the control of cell cycle progression in MCF-7 human breast cancer cells
    Oswaldo G Rodriguez-Mora
    Department of Microbiology and Immunology and the Leo Jenkins Cancer Center, Brody School of Medicine at East Carolina University, Greenville, North Carolina 27834, USA
    Cancer Res 65:5408-16. 2005
    ..To the best of our knowledge, this is the first time that CaM-KK has been reported to be involved in mammalian cell cycle regulation and that CaM-Ks are regulating breast cancer cell cycle...
  47. pmc Advances in targeting signal transduction pathways
    James A McCubrey
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, USA
    Oncotarget 3:1505-21. 2012
    ....
  48. pmc Effects of ectopic expression of NGAL on doxorubicin sensitivity
    William H Chappell
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA
    Oncotarget 3:1236-45. 2012
    ..Thus, although NGAL expression is often detected after chemotherapeutic drug treatment, it by itself, does not lead to doxorubicin resistance...
  49. ncbi request reprint Synergy between PI3K/Akt and Raf/MEK/ERK pathways in IGF-1R mediated cell cycle progression and prevention of apoptosis in hematopoietic cells
    John G Shelton
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina 27858, USA
    Cell Cycle 3:372-9. 2004
    ..These results demonstrate that the PI3K/Akt and Raf/MEK/ERK pathways are intimately involved in IGF-1R-mediated cell cycle progression and prevention of apoptosis in hematopoietic cells...
  50. ncbi request reprint Redox regulation of the calcium/calmodulin-dependent protein kinases
    Christopher J Howe
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, North Carolina 27834, USA
    J Biol Chem 279:44573-81. 2004
    ..Overall, these results demonstrate a novel mechanism by which ROI can induce CaM kinase activation in T lymphocytes...
  51. ncbi request reprint The complexity of PTEN: mutation, marker and potential target for therapeutic intervention
    Linda S Steelman
    East Carolina University, Department of Microbiology and Immunology, Brody School of Medicine, Brody Building 5N98C, Greenville, NC 27834, USA
    Expert Opin Ther Targets 8:537-50. 2004
    ..This review will discuss the role of PTEN in signal transduction and cancer as well as pharmacological approaches to combat PTEN loss in human cancer...
  52. doi request reprint Targeting the cancer initiating cell: the Achilles' heel of cancer
    James A McCubrey
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA
    Adv Enzyme Regul 51:152-62. 2011
    ..It is clear from our studies that low doses of genistein can increase the sensitivity of prostate CICs to drugs such as docetaxel and cyclopamine, two drugs either used or under consideration for prostate cancer therapy...
  53. ncbi request reprint Cell death of MCF-7 human breast cancer cells induced by EGFR activation in the absence of other growth factors
    Ruben Garcia
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, North Carolina 27858, USA
    Cell Cycle 5:1840-6. 2006
    ..These results indicate that EGFR activation by itself is not sufficient for the proliferation of breast cancer cells and suggest the existence of a mechanism that induces apoptosis upon EGFR activation...
  54. ncbi request reprint The effects of beta-estradiol on Raf activity, cell cycle progression and growth factor synthesis in the MCF-7 breast cancer cell line
    Caroline R Weinstein-Oppenheimer
    Department of Microbiology ond Immunology, Brody School of Medicine at East Carolina University, Greenville, North Carolina 27858, USA
    Cancer Biol Ther 1:256-62. 2002
    ..These findings suggest that targeting Raf may prove efficacious in breast cancer therapies...
  55. pmc p53 expression controls prostate cancer sensitivity to chemotherapy and the MDM2 inhibitor Nutlin-3
    William H Chappell
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC USA
    Cell Cycle 11:4579-88. 2012
    ..These results demonstrate that the functional status of the tumor suppressor p53 is important in the progression of prostate cancer and dictates the overall effectiveness a given drug would have on disease treatment...
  56. pmc Senescence-associated exosome release from human prostate cancer cells
    Brian D Lehmann
    Department of Anatomy and Cell Biology and Microbiology and Immunology, Leo W Jenkins Cancer Center, Brody School of Medicine, East Carolina University, Greenville, North Carolina 27834, USA
    Cancer Res 68:7864-71. 2008
    ....
  57. ncbi request reprint The epidermal growth factor receptor gene family as a target for therapeutic intervention in numerous cancers: what's genetics got to do with it?
    John G Shelton
    Brody School of Medicine at East Carolina University, Department of Microbiology and Immunology, Greenville, NC 27858, USA
    Expert Opin Ther Targets 9:1009-30. 2005
    ..Clearly, the EGF/EGFR signalling cascade has, and continues to play, an important role in the development of novel anticancer targeted therapies...
  58. ncbi request reprint Role for Raf in the entry of viruses associated with AIDS (review)
    Audy G Whitman
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 28578, USA
    Int J Oncol 25:469-80. 2004
    ..In this review we present an update on entry of various viruses commonly associated with AIDS and yet another novel way of analyzing virus entry...
  59. ncbi request reprint Neosis and its potential role in cancer development and chemoresistance
    Patrick M Navolanic
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, North Carolina 27858, USA
    Cancer Biol Ther 3:219-20. 2004
  60. ncbi request reprint Cigarette smoke concentrate inhibits Kaposi's sarcoma-associated herpesvirus infection
    Patrick W Ford
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA
    Virus Res 114:172-6. 2005
    ..Radiolabeled-binding assays indicated that CSC inhibited KSHV infection of cells at a post attachment stage of entry. Taken together, we report for the first time the ability of CSC to specifically inhibit KSHV infection of cells...
  61. ncbi request reprint Fibroblastic, hematopoietic, and hormone responsive epithelial cell lines and culture conditions for elucidation of signal transduction and drug resistance pathways by gene transfer
    Linda S Steelman
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC, USA
    Methods Mol Biol 218:185-201. 2003
    ..Furthermore, this chapter will provide a general background for understanding how gene transfer techniques can be used to elucidate signal transduction pathways as well as various pitfalls commonly encountered with their usage...
  62. pmc Roles of the Raf/MEK/ERK and PI3K/PTEN/Akt/mTOR pathways in controlling growth and sensitivity to therapy-implications for cancer and aging
    Linda S Steelman
    Department of Microbiology and Immunology, East Carolina University, Greenville, NC 27858, USA
    Aging (Albany NY) 3:192-222. 2011
    ..Finally the recently described roles of these pathways in cancer stem cells, cellular senescence and aging will be evaluated. Controlling the expression of these pathways could ameliorate human health...
  63. ncbi request reprint Increased protein expression of the PTEN tumor suppressor in the presence of constitutively active Notch-1
    William H Chappell
    Department of Microbiology and Immunology, The Brody School of Medicine at East Carolina University, Greenville, North Carolina 27834, USA
    Cell Cycle 4:1389-95. 2005
    ..These data indicate that Notch-1 can participate in cross-talk with other signaling pathways such as Ras/Raf/MEK/ERK through the regulation of the PTEN tumor suppressor...
  64. ncbi request reprint Akt as a therapeutic target in cancer
    Linda S Steelman
    Brody School of Medicine at East Carolina University, Department of Microbiology and Immunology, Greenville, NC 27858, USA
    Expert Opin Ther Targets 12:1139-65. 2008
    ....
  65. ncbi request reprint Activation of the calcium/calmodulin-dependent protein kinases as a consequence of oxidative stress
    Richard A Franklin
    Department of Microbiology and Immunology, and the Leo W Jenkins Cancer Center, Brody School of Medicine at East Carolina University, Greenville, North Carolina 27834, USA
    Antioxid Redox Signal 8:1807-17. 2006
    ..Contrasting these studies, evidence is presented that exposure of the CaM-kinases directly to hydrogen peroxide also has the apparent ability to inhibit their activity...
  66. ncbi request reprint OSU-03012 in the treatment of glioblastoma
    James A McCubrey
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Brody Building, Greenville, NC 27834, USA
    Mol Pharmacol 70:437-9. 2006
    ..Overall, these are exciting results and suggest that new more effective treatment options may be obtainable for people suffering from these deadly tumors...
  67. pmc Raf-induced vascular endothelial growth factor augments Kaposi's sarcoma-associated herpesvirus infection
    Khalief E Hamden
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA
    J Virol 78:13381-90. 2004
    ..In addition, we observed enhancement of the KSHV infection in HFF cells transfected with human VEGF(121). These results confirm the ability of Raf-induced VEGF to augment KSHV infection of cells...
  68. ncbi request reprint Combining chemo-, hormonal and targeted therapies to treat breast cancer (Review)
    Linda S Steelman
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC 27858, USA
    Mol Med Report 1:139-60. 2008
    ..This review focuses on the targeting of the Raf/MEK/ERK and PI3K/Akt pathways with small molecule inhibitors, as well as on the effects of conventional chemo- and hormonal therapies in the treatment of breast cancer...
  69. ncbi request reprint Biology of Kaposi's sarcoma-associated herpesvirus
    Benjaman A Bryan
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA
    Front Biosci 10:2882-91. 2005
    ..In this chapter, we delineate some of the latest findings about KSHV infection and pathogenesis...
  70. ncbi request reprint Reactive oxygen species-induced activation of the MAP kinase signaling pathways
    James A McCubrey
    Department of Microbiology and Immunology, and the Leo W Jenkins Cancer Center, Brody School of Medicine at East Carolina University, Greenville, North Carolina 27834, USA
    Antioxid Redox Signal 8:1775-89. 2006
    ..The purpose of this review is to provide the reader with an overall understanding and appreciation of oxidative stress-induced MAPK signaling...
  71. ncbi request reprint Targeting the Raf kinase cascade in cancer therapy--novel molecular targets and therapeutic strategies
    John T Lee
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Greenville, NC, USA
    Expert Opin Ther Targets 6:659-78. 2002
    ..Finally, the potential therapeutic promise of many of these compounds is addressed in a manner that encompasses the complexities of MAPK signal transduction, in addition to concerns surrounding the development of drug resistance...
  72. ncbi request reprint Acquired immunodeficiency syndrome associated lymphoma
    Fred E Bertrand
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, 600 Moye Blvd, Greenville, NC 27834, USA
    Front Biosci 10:2972-7. 2005
    ..The treatment and etiology of lymphoma is a burgeoning issue in the care of HIV positive populations. This review will provide a basic overview of the association between HIV and lymphoma...
  73. ncbi request reprint Polyphenols in breast cancer treatment
    Richard A Franklin
    Department of Microbiology and Immunology, Brody School of Medicine at East Carolina University, Brody Building, Greenville, North Carolina 27834, USA
    Cancer Biol Ther 6:62-3. 2007
  74. ncbi request reprint Raf-induced cell cycle progression in human TF-1 hematopoietic cells
    Fumin Chang
    Department of Microbiology and Immunology Brody School of Medicine at East Carolina University Greenville, North Carolina 27858, USA
    Cell Cycle 1:220-6. 2002
    ..Furthermore, the differences that the various Raf isoforms have on Cdk4 activity and other cell cycle regulatory molecules can be determined in these cells...
  75. ncbi request reprint Integrin signaling links protein kinase Cepsilon to the protein kinase B/Akt survival pathway in recurrent prostate cancer cells
    Daqing Wu
    Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University, Greenville, NC 27858, USA
    Oncogene 23:8659-72. 2004
    ....
  76. ncbi request reprint Pharmacological breast cancer therapy (review)
    Patrick M Navolanic
    Department of Microbiology and Immunology, East Carolina University, Greenville, NC 27858, USA
    Int J Oncol 27:1341-4. 2005
    ..These agents inhibit breast cancer progression by a variety of different mechanisms. This review describes each of these drugs and the varying effects each of them have upon breast cancer cells...
  77. ncbi request reprint BAY-43-9006 Bayer/Onyx
    John T Lee
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, East Fifth Street, Greenville, NC 27858 4553, USA
    Curr Opin Investig Drugs 4:757-63. 2003
    ..A US IND was filed in May 2000 and by February 2003 BAY-43-9006 was in phase II trials, with phase III trials expected to begin later in 2003...
  78. ncbi request reprint Calcium/calmodulin-dependent protein kinases as potential targets in cancer therapy
    Oswaldo Rodriguez-Mora
    Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA
    Expert Opin Ther Targets 9:791-808. 2005
    ..Based on these roles the authors suggest that inhibition of the CaM-kinases not only has the potential to sensitise cancer cells, but also has the potential to induce cytostasis in these cells...
  79. pmc Microarray and pathway analysis reveals decreased CDC25A and increased CDC42 associated with slow growth of BCL2 overexpressing immortalized breast cell line
    Jacquelyn M Long
    Department of Biology, East Carolina University, Greenville, North Carolina 27858, USA
    Cell Cycle 7:3062-73. 2008
    ..Increased levels of GTPase Cdc42 could be adding to this effect. PAGE and IPA are valuable tools for microarray analysis, but protein expression results do not always follow mRNA expression results...
  80. pmc Proapoptotic activity and chemosensitizing effect of the novel Akt inhibitor (2S)-1-(1H-Indol-3-yl)-3-[5-(3-methyl-2H-indazol-5-yl)pyridin-3-yl]oxypropan2-amine (A443654) in T-cell acute lymphoblastic leukemia
    Federica Fala
    Department of Human Anatomical Sciences, University of Bologna, 40126 Bologna, Italy
    Mol Pharmacol 74:884-95. 2008
    ..Because activated AKT is seen in a large percentage of patients with T-ALL, A443654, either alone or in combination with existing drugs, may be a useful therapy for primary and drug-resistant T-ALL...
  81. ncbi request reprint Detection of BRAF gene mutation in primary choroidal melanoma tissue
    Grazia Malaponte
    Department of Biomedical Sciences, Vittorio Emanuele II Hospital, Catania, Italy
    Cancer Biol Ther 5:225-7. 2006
    ..The presence of V600E mutation indicates that the RAF/MEK/ERK pathway, in addition to cutaneous melanoma progression, may play a role in the choroidal melanoma development...
  82. pmc PI3K activation is associated with intracellular sodium/iodide symporter protein expression in breast cancer
    Katherine A B Knostman
    Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio 43210, USA
    BMC Cancer 7:137. 2007
    ..We previously identified PI3K signaling as important in NIS upregulation in transgenic mouse models of breast cancer, and the PI3K pathway is commonly activated in human breast cancer...
  83. pmc Overcoming resistance to molecularly targeted anticancer therapies: Rational drug combinations based on EGFR and MAPK inhibition for solid tumours and haematologic malignancies
    Giampaolo Tortora
    Dipartimento di Endocrinologia e Oncologia Molecolare e Clinica, Universita di Napoli Federico II, Naples, Italy
    Drug Resist Updat 10:81-100. 2007
    ....
  84. ncbi request reprint Development of a conditional in vivo model to evaluate the efficacy of small molecule inhibitors for the treatment of Raf-transformed hematopoietic cells
    Marina Konopleva
    Department of Blood and Marrow Transplantation, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Cancer Res 65:9962-70. 2005
    ..This DeltaRaf:ER system can serve as a preclinical model to evaluate the effects of signal transduction inhibitors which target the Raf and MEK proteins...
  85. ncbi request reprint Selective killing of adriamycin-resistant (G2 checkpoint-deficient and MRP1-expressing) cancer cells by docetaxel
    Zoya N Demidenko
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York, USA
    Cancer Res 65:4401-7. 2005
    ..We propose a therapeutic strategy to prevent normal cells from entering mitosis while increasing apoptosis selectively in mitotic cancer cells...
  86. ncbi request reprint A novel ring-substituted diindolylmethane,1,1-bis[3'-(5-methoxyindolyl)]-1-(p-t-butylphenyl) methane, inhibits extracellular signal-regulated kinase activation and induces apoptosis in acute myelogenous leukemia
    Rooha Contractor
    Department of Blood and Marrow Transplantation, University of Texas M D Anderson Cancer Center, Houston 77030, USA
    Cancer Res 65:2890-8. 2005
    ..Together, these findings suggest that diindolylmethanes are a new class of compounds that selectively induce apoptosis in AML cells through the modulation of the extracellular signal-regulated kinase and PPARgamma signaling pathways...
  87. ncbi request reprint Kinase-addiction and bi-phasic sensitivity-resistance of Bcr-Abl- and Raf-1-expressing cells to imatinib and geldanamycin
    Zoya N Demidenko
    Brander Cancer Research Institute, New York Medical College, Valhalla, New York, USA
    Cancer Biol Ther 4:484-90. 2005
    ..Although Bcr-Abl renders cells hyper-sensitive, an excess of Bcr-Abl results in resistance (due to the remaining activity). We discuss therapeutic approaches to overcome bi-phasic resistance to mechanisms-based agents...
  88. ncbi request reprint Absence of BRAF gene mutation in non-melanoma skin tumors
    Massimo Libra
    Department of Biomedical Sciences, University of Catania, Italy
    Cell Cycle 5:968-70. 2006
    ..Our results indicate that the BRAF gene does not appear to be frequently mutated in nonmelanoma skin tumors such as BCC. These data suggest that other gene alterations may cause tumor development...
  89. ncbi request reprint Signaling through 3',5'-cyclic adenosine monophosphate and phosphoinositide-3 kinase induces sodium/iodide symporter expression in breast cancer
    Katherine A B Knostman
    Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio 43210, USA
    J Clin Endocrinol Metab 89:5196-203. 2004
    ..This study is the first to demonstrate that NIS expression is induced by cAMP and/or PI3K in breast cancer both in vivo and in vitro...