D J Lampe

Summary

Affiliation: Duquesne University
Country: USA

Publications

  1. ncbi Hyperactive transposase mutants of the Himar1 mariner transposon
    D J Lampe
    Department of Biological Sciences, Duquesne University, Pittsburgh, PA 15282, USA
    Proc Natl Acad Sci U S A 96:11428-33. 1999
  2. ncbi Loss of transposase-DNA interaction may underlie the divergence of mariner family transposable elements and the ability of more than one mariner to occupy the same genome
    D J Lampe
    Department of Biological Sciences, Duquesne University, Pittsburgh, PA 15282, USA
    Mol Biol Evol 18:954-61. 2001
  3. ncbi Bacterial genetic methods to explore the biology of mariner transposons
    David J Lampe
    Department of Biological Sciences, Duquesne University, 600 Forbes Ave, Pittsburgh, PA 15116, USA
    Genetica 138:499-508. 2010
  4. ncbi Recent horizontal transfer of mellifera subfamily mariner transposons into insect lineages representing four different orders shows that selection acts only during horizontal transfer
    David J Lampe
    Department of Biological Sciences, Duquesne University
    Mol Biol Evol 20:554-62. 2003
  5. ncbi A bacterial genetic screen identifies functional coding sequences of the insect mariner transposable element Famar1 amplified from the genome of the earwig, Forficula auricularia
    Elizabeth G Barry
    Department of Biological Sciences, Duquesne University, Pittsburgh, PA 15282, USA
    Genetics 166:823-33. 2004
  6. ncbi Secretion of anti-Plasmodium effector proteins from a natural Pantoea agglomerans isolate by using PelB and HlyA secretion signals
    Dawn C Bisi
    Duquesne University, Department of Biological Sciences, 600 Forbes Ave, Pittsburgh, PA 15282, USA
    Appl Environ Microbiol 77:4669-75. 2011
  7. ncbi Early intermediates of mariner transposition: catalysis without synapsis of the transposon ends suggests a novel architecture of the synaptic complex
    Karen Lipkow
    Department of Biochemistry, University of Oxford, United Kingdom
    Mol Cell Biol 24:8301-11. 2004
  8. ncbi The N-terminus of Himar1 mariner transposase mediates multiple activities during transposition
    Matthew G Butler
    Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA
    Genetica 127:351-66. 2006
  9. ncbi Hyperactive Himar1 transposase mediates transposition in cell culture and enhances gene expression in vivo
    Annahita Keravala
    Department of Molecular Genetics and Biochemistry, School of Medicine, University of Pittsburgh, PA 15261, USA
    Hum Gene Ther 17:1006-18. 2006
  10. ncbi Analysis of gene function in bacterial pathogens by GAMBIT
    Brian J Akerley
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Methods Enzymol 358:100-8. 2002

Research Grants

  1. Secretion systems for anti-malarial bacterial strains
    David Lampe; Fiscal Year: 2006

Collaborators

Detail Information

Publications10

  1. ncbi Hyperactive transposase mutants of the Himar1 mariner transposon
    D J Lampe
    Department of Biological Sciences, Duquesne University, Pittsburgh, PA 15282, USA
    Proc Natl Acad Sci U S A 96:11428-33. 1999
    ..Mutants like those described herein should enable sophisticated analysis of the biochemistry of mariner transposition and should improve the use of these elements as genetic tools, both in vivo and in vitro...
  2. ncbi Loss of transposase-DNA interaction may underlie the divergence of mariner family transposable elements and the ability of more than one mariner to occupy the same genome
    D J Lampe
    Department of Biological Sciences, Duquesne University, Pittsburgh, PA 15282, USA
    Mol Biol Evol 18:954-61. 2001
    ..We further propose a mechanism to explain mariner diversification based on this phenomenon...
  3. ncbi Bacterial genetic methods to explore the biology of mariner transposons
    David J Lampe
    Department of Biological Sciences, Duquesne University, 600 Forbes Ave, Pittsburgh, PA 15116, USA
    Genetica 138:499-508. 2010
    ..Finally, it is possible to uncover functional transposase genes directly from genomes given a suitable bacterial genetic screen...
  4. ncbi Recent horizontal transfer of mellifera subfamily mariner transposons into insect lineages representing four different orders shows that selection acts only during horizontal transfer
    David J Lampe
    Department of Biological Sciences, Duquesne University
    Mol Biol Evol 20:554-62. 2003
    ....
  5. ncbi A bacterial genetic screen identifies functional coding sequences of the insect mariner transposable element Famar1 amplified from the genome of the earwig, Forficula auricularia
    Elizabeth G Barry
    Department of Biological Sciences, Duquesne University, Pittsburgh, PA 15282, USA
    Genetics 166:823-33. 2004
    ..Finally, this method of isolating active copies can be used to isolate other novel active transposons without resorting to reconstruction of ancestral sequences...
  6. ncbi Secretion of anti-Plasmodium effector proteins from a natural Pantoea agglomerans isolate by using PelB and HlyA secretion signals
    Dawn C Bisi
    Duquesne University, Department of Biological Sciences, 600 Forbes Ave, Pittsburgh, PA 15282, USA
    Appl Environ Microbiol 77:4669-75. 2011
    ..Importantly, those strains that secreted efficiently grew as well as wild-type strains under laboratory conditions and, thus, may be expected to be competitive with the native microbiota in the environment of the mosquito midgut...
  7. ncbi Early intermediates of mariner transposition: catalysis without synapsis of the transposon ends suggests a novel architecture of the synaptic complex
    Karen Lipkow
    Department of Biochemistry, University of Oxford, United Kingdom
    Mol Cell Biol 24:8301-11. 2004
    ..We discuss the role of this single-end activity in generating the mutations that inactivate the vast majority of mariner elements in eukaryotes...
  8. ncbi The N-terminus of Himar1 mariner transposase mediates multiple activities during transposition
    Matthew G Butler
    Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA
    Genetica 127:351-66. 2006
    ....
  9. ncbi Hyperactive Himar1 transposase mediates transposition in cell culture and enhances gene expression in vivo
    Annahita Keravala
    Department of Molecular Genetics and Biochemistry, School of Medicine, University of Pittsburgh, PA 15261, USA
    Hum Gene Ther 17:1006-18. 2006
    ..These data suggest that vectors based on the Himar1 transposable element, in conjunction with the hyperactive mutant transposase C9, may be suitable vectors for gene therapy applications...
  10. ncbi Analysis of gene function in bacterial pathogens by GAMBIT
    Brian J Akerley
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Methods Enzymol 358:100-8. 2002

Research Grants1

  1. Secretion systems for anti-malarial bacterial strains
    David Lampe; Fiscal Year: 2006
    ..agglomerans that secrete antimalarial proteins can block the transmission of malaria from mosquitoes to their hosts in a rodent malaria model system. Both Type I and Type II secretion systems will be developed and tested. ..