J H Toyn

Summary

Affiliation: DuPont Pharmaceuticals Company
Country: USA

Publications

  1. ncbi request reprint A counterselection for the tryptophan pathway in yeast: 5-fluoroanthranilic acid resistance
    J H Toyn
    Department of Applied Biotechnology, DuPont Pharmaceuticals Co, Wilmington, DE 19880, USA
    Yeast 16:553-60. 2000
  2. ncbi request reprint A yeast genetic assay for caspase cleavage of the amyloid-beta precursor protein
    P L Gunyuzlu
    DuPont Pharmaceuticals, Experimental Station, Wilmington, Delaware 19880 0336, USA
    Mol Biotechnol 15:29-37. 2000
  3. ncbi request reprint Saccharomyces cerevisiae is capable of de Novo pantothenic acid biosynthesis involving a novel pathway of beta-alanine production from spermine
    W H White
    Department of Applied Biotechnology, DuPont Pharmaceuticals Company, Wilmington, Delaware 19880 0336, USA
    J Biol Chem 276:10794-800. 2001
  4. pmc A Bub2p-dependent spindle checkpoint pathway regulates the Dbf2p kinase in budding yeast
    D Fesquet
    Division of Yeast Genetics, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    EMBO J 18:2424-34. 1999
  5. ncbi request reprint DNA replication is completed in Saccharomyces cerevisiae cells that lack functional Cdc14, a dual-specificity protein phosphatase
    P J Fitzpatrick
    Division of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, UK
    Mol Gen Genet 258:437-41. 1998
  6. pmc DBF2 protein kinase binds to and acts through the cell cycle-regulated MOB1 protein
    S I Komarnitsky
    Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham 03824, USA
    Mol Cell Biol 18:2100-7. 1998
  7. pmc DBF2, a cell cycle-regulated protein kinase, is physically and functionally associated with the CCR4 transcriptional regulatory complex
    H Y Liu
    Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham, NH 03824, USA
    EMBO J 16:5289-98. 1997
  8. pmc The Swi5 transcription factor of Saccharomyces cerevisiae has a role in exit from mitosis through induction of the cdk-inhibitor Sic1 in telophase
    J H Toyn
    Division of Yeast Genetics, National Institute for Medical Research, London, United Kingdom
    Genetics 145:85-96. 1997
  9. pmc Rme1, a negative regulator of meiosis, is also a positive activator of G1 cyclin gene expression
    W M Toone
    Division of Yeast Genetics, National Institute for Medical Research, London, UK
    EMBO J 14:5824-32. 1995
  10. pmc Spo12 is a limiting factor that interacts with the cell cycle protein kinases Dbf2 and Dbf20, which are involved in mitotic chromatid disjunction
    J H Toyn
    Laboratory of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, United Kingdom
    Genetics 135:963-71. 1993

Collaborators

  • FRANCIS LUCA
  • MARK E WINEY
  • CLYDE DENIS
  • L H Johnston
  • W H White
  • P L Gunyuzlu
  • A L Johnson
  • P J Fitzpatrick
  • D Fesquet
  • S I Komarnitsky
  • B L Aerne
  • Y C Chiang
  • H Y Liu
  • G F Hollis
  • W M Toone
  • G L Davis
  • K M Kramer
  • J B Millar
  • J E Shea
  • J D Donovan
  • J Chen
  • M P Draper
  • D Stuart
  • G R Banks
  • C Wittenberg

Detail Information

Publications16

  1. ncbi request reprint A counterselection for the tryptophan pathway in yeast: 5-fluoroanthranilic acid resistance
    J H Toyn
    Department of Applied Biotechnology, DuPont Pharmaceuticals Co, Wilmington, DE 19880, USA
    Yeast 16:553-60. 2000
    ..In addition, novel tryptophan auxotrophs can be selected using 5-fluoroanthranilic acid...
  2. ncbi request reprint A yeast genetic assay for caspase cleavage of the amyloid-beta precursor protein
    P L Gunyuzlu
    DuPont Pharmaceuticals, Experimental Station, Wilmington, Delaware 19880 0336, USA
    Mol Biotechnol 15:29-37. 2000
    ..The activation of caspase-3 involved a mechanism that was independent of aspartic acid residue 175 at the cleavage site normally required for processing of caspase-3...
  3. ncbi request reprint Saccharomyces cerevisiae is capable of de Novo pantothenic acid biosynthesis involving a novel pathway of beta-alanine production from spermine
    W H White
    Department of Applied Biotechnology, DuPont Pharmaceuticals Company, Wilmington, Delaware 19880 0336, USA
    J Biol Chem 276:10794-800. 2001
    ..cerevisiae and raise questions about these pathways in other organisms...
  4. pmc A Bub2p-dependent spindle checkpoint pathway regulates the Dbf2p kinase in budding yeast
    D Fesquet
    Division of Yeast Genetics, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    EMBO J 18:2424-34. 1999
    ..Our data now point to the protein kinase Mps1p triggering a new parallel branch of the spindle checkpoint in which Bub2p blocks Dbf2p function...
  5. ncbi request reprint DNA replication is completed in Saccharomyces cerevisiae cells that lack functional Cdc14, a dual-specificity protein phosphatase
    P J Fitzpatrick
    Division of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, UK
    Mol Gen Genet 258:437-41. 1998
    ..Furthermore, in a promoter shut-off experiment, cells lacking Cdc14 appear to carry out a normal S phase. Thus Cdc14 functions mainly in late mitosis and it has no essential role in S phase...
  6. pmc DBF2 protein kinase binds to and acts through the cell cycle-regulated MOB1 protein
    S I Komarnitsky
    Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham 03824, USA
    Mol Cell Biol 18:2100-7. 1998
    ..We discuss models concerning the role of DBF2 and MOB1 in controlling the telophase/G1 transition...
  7. pmc DBF2, a cell cycle-regulated protein kinase, is physically and functionally associated with the CCR4 transcriptional regulatory complex
    H Y Liu
    Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham, NH 03824, USA
    EMBO J 16:5289-98. 1997
    ..These data indicate that DBF2 is involved in the control of gene expression and suggest that the CCR4 complex regulates transcription during the late mitotic part of the cell cycle...
  8. pmc The Swi5 transcription factor of Saccharomyces cerevisiae has a role in exit from mitosis through induction of the cdk-inhibitor Sic1 in telophase
    J H Toyn
    Division of Yeast Genetics, National Institute for Medical Research, London, United Kingdom
    Genetics 145:85-96. 1997
    ..Second, both are hypersensitive to overexpression of the B cyclin CLB2. Thus Swi5-dependent activation of the SIC1 gene contributes to the deactivation of the B cyclin kinase, and hence exit from mitosis...
  9. pmc Rme1, a negative regulator of meiosis, is also a positive activator of G1 cyclin gene expression
    W M Toone
    Division of Yeast Genetics, National Institute for Medical Research, London, UK
    EMBO J 14:5824-32. 1995
    ..Thus Rme1 may act both to promote mitosis, by activating CLN2 expression, and prevent meiosis, by repressing IME1 expression...
  10. pmc Spo12 is a limiting factor that interacts with the cell cycle protein kinases Dbf2 and Dbf20, which are involved in mitotic chromatid disjunction
    J H Toyn
    Laboratory of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, United Kingdom
    Genetics 135:963-71. 1993
    ..Conventional assays for chromosome loss confirmed this proposal...
  11. pmc The Dbf2 and Dbf20 protein kinases of budding yeast are activated after the metaphase to anaphase cell cycle transition
    J H Toyn
    Laboratory of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, UK
    EMBO J 13:1103-13. 1994
    ..Thus, Dbf2/Dbf20 kinase activity is precisely regulated in the cell cycle by a post-translational mechanism and phosphorylates its target substrates for an event that occurs during anaphase and/or telophase...
  12. ncbi request reprint P40SDB25, a putative CDK inhibitor, has a role in the M/G1 transition in Saccharomyces cerevisiae
    J D Donovan
    Laboratory of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, UK
    Genes Dev 8:1640-53. 1994
    ..Our data suggest that Sdb25 functions in a pathway in late mitosis leading to the down-regulation of Cdc28 kinase activity as cells traverse the M/G1 boundary...
  13. pmc The budding yeast U5 snRNP Prp8 is a highly conserved protein which links RNA splicing with cell cycle progression
    J E Shea
    Laboratory of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, UK
    Nucleic Acids Res 22:5555-64. 1994
    ..This data suggests a hitherto unexpected link between pre-mRNA splicing and the cell cycle...
  14. pmc Swi5 controls a novel wave of cyclin synthesis in late mitosis
    B L Aerne
    Division of Yeast Genetics, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    Mol Biol Cell 9:945-56. 1998
    ..Consistent with this a synthetic interaction was observed between pho85delta and strains deleted for SIC1, SWI5, and SPO12. These and other studies support the notion that the M/G1 switch is a major cell cycle transition...
  15. ncbi request reprint The activation of DNA replication in yeast
    J H Toyn
    Laboratory of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, UK
    Trends Biochem Sci 20:70-3. 1995
    ..In this review, we concentrate largely on those genes on proteins that are required for initiation of DNA replication and for which there is some evidence for a role at origins...
  16. ncbi request reprint The cell-cycle-regulated budding yeast gene DBF2, encoding a putative protein kinase, has a homologue that is not under cell-cycle control
    J H Toyn
    Laboratory of Cell Propagation, National Institute for Medical Research, The Ridgeway, Mill Hill, London, U K
    Gene 104:63-70. 1991
    ..Assuming there is no translational control, the differential expression of the mRNAs would result in a cell-cycle fluctuation of the relative levels of the gene products, which may constitute a novel form of regulation...