Genomes and Genes
J D York
Affiliation: Duke University Medical Center
- A phospholipase C-dependent inositol polyphosphate kinase pathway required for efficient messenger RNA exportJ D York
Department of Pharmacology, Duke University Medical Center, DUMC 3813, Durham, NC 27710, USA
Science 285:96-100. 1999..The common downstream effects of mutations in each component were deficiencies in IP6 synthesis and messenger RNA export, indicating a role for IP6 in GLE1 function and messenger RNA export...
- A role for nuclear inositol 1,4,5-trisphosphate kinase in transcriptional controlA R Odom
Departments of Pharmacology and Cancer Biology and of Biochemistry, Duke University Medical Center, DUMC 3813, Durham, NC 27710, USA
Science 287:2026-9. 2000..Thus, the phospholipase C pathway produces multiple IP messengers that modulate distinct nuclear processes. The results reveal a direct mechanism by which activation of IP signaling may control gene expression...
- SAC1-like domains of yeast SAC1, INP52, and INP53 and of human synaptojanin encode polyphosphoinositide phosphatasesS Guo
Departments of Pharmacology and Cancer Biology and of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 274:12990-5. 1999..Additionally, the tethering of PPIPase and 5-phosphatase activities indicate a novel mechanism by which concerted phosphoinositide hydrolysis participates in membrane trafficking...
- Tyrosine 1101 of Tie2 is the major site of association of p85 and is required for activation of phosphatidylinositol 3-kinase and AktC D Kontos
Departments of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
Mol Cell Biol 18:4131-40. 1998....
- Cloning and characterization of a mammalian lithium-sensitive bisphosphate 3'-nucleotidase inhibited by inositol 1,4-bisphosphateB D Spiegelberg
Departments of Pharmacology and Cancer Biology and Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 274:13619-28. 1999..We propose that inhibition of human BPntase may account for lithium-induced nephrotoxicity, which may be overcome by supplementation of current therapeutic regimes with inhibitors of nucleotide biosynthesis, such as methionine...
- Phosphatidylinositol 4,5-bisphosphate functions as a second messenger that regulates cytoskeleton-plasma membrane adhesionD Raucher
Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
Cell 100:221-8. 2000..Our study suggests that plasma membrane PIP2 controls dynamic membrane functions and cell shape by locally increasing and decreasing the adhesion between the actin-based cortical cytoskeleton and the plasma membrane...
- INP51, a yeast inositol polyphosphate 5-phosphatase required for phosphatidylinositol 4,5-bisphosphate homeostasis and whose absence confers a cold-resistant phenotypeL E Stolz
Departments of Pharmacology and Cancer Biology and of Biochemistry, Duke Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 273:11852-61. 1998..In addition, we define a novel role for a 5-phosphatase loss of function mutant that improves the growth of cells at colder temperatures without alteration of growth at normal temperatures, which may have useful commercial applications...
- Biochemical and functional characterization of inositol 1,3,4,5, 6-pentakisphosphate 2-kinasesE B Ives
Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Biol Chem 275:36575-83. 2000..This includes two conserved motifs: E(i/l/m)KPKWL(t/y) and LXMTLRDV(t/g)(l/c)(f/y)I. Our data suggest that the mechanism for IP(6) production is conserved across species...
- Pleiotropic alterations in lipid metabolism in yeast sac1 mutants: relationship to "bypass Sec14p" and inositol auxotrophyM P Rivas
Department of Cell Biology, University of Alabama at Birmingham, Birmingham, Alabama 35294 0005, USA
Mol Biol Cell 10:2235-50. 1999..Finally, we determine that CDP-choline pathway activity contributes to the inositol auxotrophy of sac1 strains in a novel manner that does not involve obvious defects in transcriptional expression of the INO1 gene...
- Identification and characterization of an essential family of inositol polyphosphate 5-phosphatases (INP51, INP52 and INP53 gene products) in the yeast Saccharomyces cerevisiaeL E Stolz
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
Genetics 148:1715-29. 1998....
- Specificity determinants in phosphoinositide dephosphorylation: crystal structure of an archetypal inositol polyphosphate 5-phosphataseY Tsujishita
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Cell 105:379-89. 2001..Based on the structure, a series of mutants are described that exhibit altered substrate specificity providing general determinants for substrate recognition...