Research Topics
| ADAM P WAXSummaryAffiliation: Duke University Medical Center Country: USA Publications
Research Grants
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Detail Information
Publications
In situ detection of neoplastic transformation and chemopreventive effects in rat esophagus epithelium using angle-resolved low-coherence interferometryAdam Wax
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
Cancer Res 63:3556-9. 2003..This technique also may provide a new screening tool for intraepithelial neoplasia...- In situ nuclear morphology measurements using light scattering as biomarkers of neoplastic change in animal models of carcinogenesisAdam Wax
Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, Duke University, Durham, NC, USA
Dis Markers 25:291-301. 2008..These models provide the opportunity to develop biomarkers and test chemopreventive and therapy strategies before application in humans... 
Nuclear morphology measurements with angle-resolved low coherence interferometry for application to cell biology and early cancer detectionAdam Wax
Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, Duke University, Durham, NC 27708, USA
Anal Cell Pathol (Amst) 34:207-22. 2011..Experiments with in vitro cell samples also show the utility of a/LCI in observing structural changes due to environmental stimuli as well as detecting apoptosis due to chemotherapeutic agents...
Rapid, depth-resolved light scattering measurements using Fourier domain, angle-resolved low coherence interferometryJohn W Pyhtila
Department of Biomedical Engineering and the Fitzpatrick Center for Photonics and Communication Systems, Duke University, Durham, NC 27708
Opt Express 12:6178-83. 2004..The data are used to determine the microsphere size with good accuracy. Future clinical application to measuring the size of cell nuclei in living epithelial tissues using backscattered light is discussed...- Prospective grading of neoplastic change in rat esophagus epithelium using angle-resolved low-coherence interferometryAdam Wax
Duke University, Department of Biomedical Engineering, Durham, North Carolina 27708, USA
J Biomed Opt 10:051604. 2005..We demonstrate that a/LCI is highly effective for monitoring neoplastic change and can be applied to assessing the efficacy of chemopreventive agents in the rat esophagus... - Fourier-domain low-coherence interferometry for light-scattering spectroscopyAdam Wax
Department of Biomedical Engineering and the Fitzpatrick Center for Photonics, Duke University, Durham, North Carolina 27708, USA
Opt Lett 28:1230-2. 2003..Application of the technique to probing the size of cell nuclei in living epithelial tissues is discussed... - Experimental verification of T-matrix-based inverse light scattering analysis for assessing structure of spheroids as models of cell nucleiCyrus Amoozegar
Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, Duke University, Durham, North Carolina 27708, USA
Appl Opt 48:D20-5. 2009..In comparison, analysis using the T-matrix method is highly accurate and more reliable yet requires measurements from only a single orientation... - Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometryJohn W Pyhtila
Duke University, Department of Biomedical Engineering, Fitzpatrick Center for Photonics, Durham, North Carolina 27708
J Biomed Opt 11:34022. 2006..The a/LCI results show that the impact of coherent scattering on nuclear morphology measurements can be eliminated through data filtering... - Measuring morphological features using light-scattering spectroscopy and Fourier-domain low-coherence interferometryFrancisco E Robles
Department of Medical Physics, Duke University, Durham, North Carolina 27708, USA
Opt Lett 35:360-2. 2010..The spectral information from localized regions in the sample is used to determine scatterer structure. The results show that the two scatterer populations can be differentiated using LSS and fLCI... - Detecting precancerous lesions in the hamster cheek pouch using spectroscopic white-light optical coherence tomography to assess nuclear morphology via spectral oscillationsRobert N Graf
Duke University, Department of Biomedical Engineering, 136 Hudson Hall, Durham, North Carolina 27708, USA
J Biomed Opt 14:064030. 2009..The structural measurements show a highly statistically significant difference between untreated (normal) and treated (hyperplastic/dysplastic) tissues, indicating the potential utility of this approach as a diagnostic method... - Application of the T-matrix method to determine the structure of spheroidal cell nuclei with angle-resolved light scatteringMichael G Giacomelli
Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, Duke University, Durham, North Carolina 27708, USA
Opt Lett 33:2452-4. 2008..These improvements represent a significant upgrade for the a/LCI technique, expanding both the range of tissue in which it is applicable and potentially increasing its value as a diagnostic tool... - Molecular imaging and quantitative measurement of epidermal growth factor receptor expression in live cancer cells using immunolabeled gold nanoparticlesMatthew J Crow
Department of Biomedical Engineering, Duke University, Box 3808, Durham, NC 27710, USA
AJR Am J Roentgenol 192:1021-8. 2009..The goal of this study was to assess whether immunolabeled nanoparticle biomarkers are comparable to fluorescent marker imaging in measuring epidermal growth factor receptor (EGFR) expression... - Dual window method for processing spectroscopic optical coherence tomography signals with simultaneously high spectral and temporal resolutionFrancisco Robles
Department of Medical Physics, Duke University, Durham, NC 27708, USA
Opt Express 17:6799-812. 2009..The DW method yields TFDs that maintain high spectral and temporal resolution and are free from the artifacts and limitations commonly observed with other processing methods... - Scanning fiber angle-resolved low coherence interferometryYizheng Zhu
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
Opt Lett 34:3196-8. 2009..We characterize the optical performance of the probe and demonstrate the ability to execute depth-resolved sizing with subwavelength accuracy by using a double-layer phantom containing two sizes of polystyrene microspheres... - In situ detection of nuclear atypia in Barrett's esophagus by using angle-resolved low-coherence interferometryJohn W Pyhtila
Department of Biomedical Engineering and the Fitzpatrick Institute for Photonics, Duke University Medical Center, Durham, NC, USA
Gastrointest Endosc 65:487-91. 2007..Monitoring of patients with Barrett's esophagus (BE) for dysplasia, currently done by systematic biopsy, can be improved through increasing the proportion of at-risk tissue examined... - Light scattering measurements of subcellular structure provide noninvasive early detection of chemotherapy-induced apoptosisKevin J Chalut
Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, Duke University, Durham, North Carolina 27708, USA
Cancer Res 69:1199-204. 2009..These results suggest that a/LCI may have utility in detecting early apoptotic events for both clinical and basic science applications... - Two-step-only phase-shifting interferometry with optimized detector bandwidth for microscopy of live cellsNatan T Shaked
Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, Duke University Durham, North Carolina 27708, USA
Opt Express 17:15585-91. 2009..Experimental and theoretical comparisons between the proposed method and these traditional interferometric approaches are given. The method is experimentally demonstrated via phase microscopy of live human skin cancer cells... - Parallel on-axis holographic phase microscopy of biological cells and unicellular microorganism dynamicsNatan T Shaked
1Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, Duke University, Durham, North Carolina 27708, USA
Appl Opt 49:2872-8. 2010..The method is demonstrated experimentally through phase microscopy of neurons and unicellular microorganisms... - Simultaneous two-wavelength transmission quantitative phase microscopy with a color cameraMatthew T Rinehart
Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, Duke University, Durham, North Carolina 27708, USA
Opt Lett 35:2612-4. 2010..In addition, the phase noise contribution arising from spectral channel crosstalk on the color camera is quantified... - Rational selection of gold nanorod geometry for label-free plasmonic biosensorsGreg J Nusz
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
ACS Nano 3:795-806. 2009..Further, we discuss how the model can be utilized to guide the development of future generations of LSPR biosensors... - Spectral-domain differential interference contrast microscopyYizheng Zhu
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
Opt Lett 36:430-2. 2011..Live cardiomyocytes were also imaged, achieving a resolution of 36 pm for pathlength gradient measurements. The dynamics of cardiomyocyte contraction were recorded with high sensitivity at selected sites on the cells... - Dual-interference-channel quantitative-phase microscopy of live cell dynamicsNatan T Shaked
Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, Duke University, Durham, NC 27708, USA
Opt Lett 34:767-9. 2009.... - Fourier-domain angle-resolved low coherence interferometry through an endoscopic fiber bundle for light-scattering spectroscopyJohn W Pyhtila
Department of Biomedical Engineering and the Fitzpatrick Center for Photonics and Communication Systems, Duke University, Durham, North Carolina 27708, USA
Opt Lett 31:772-4. 2006..The data collected are processed in real time, producing a depth-resolved angular scattering distribution in 0.37 s. The data are used to determine the sizes of polystyrene microspheres with subwavelength precision and accuracy... - Improved interferometric detection of scattered light with a 4f imaging systemJohn W Pyhtila
Department of Biomedical Engineering and the Fitzpatrick Center for Photonics, Duke University, Durham, North Carolina 27708, USA
Appl Opt 44:1785-91. 2005..Of the three systems analyzed, the 4f imaging system is determined to be most effective, because it accurately reproduces both the phase and the amplitude of the scattered field at the detector... - Photothermal optical coherence tomography of epidermal growth factor receptor in live cells using immunotargeted gold nanospheresMelissa C Skala
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
Nano Lett 8:3461-7. 2008..This technique could potentially augment confocal and multiphoton microscopy as a method for deep-tissue, depth-resolved molecular imaging with relatively high resolution and target sensitivity, without photobleaching or cytotoxicity... - Application of Mie theory to assess structure of spheroidal scattering in backscattering geometriesKevin J Chalut
Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, Duke University, Durham, NC 27708, USA
J Opt Soc Am A Opt Image Sci Vis 25:1866-74. 2008..Over this wide range of parameters, our results indicate that this method provides a good estimate of spheroidal structure... - Low-cost, scalable laser scanning module for real-time reflectance and fluorescence confocal microscopyDerrick R Chou
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
Appl Opt 44:2013-8. 2005..This laser scanning module is a flexible, scalable, high-speed alternative to commercial laser scanning systems suitable for applications requiring a simple imaging tool and for teaching laboratories... - Label-free, high-throughput measurements of dynamic changes in cell nuclei using angle-resolved low coherence interferometryKevin J Chalut
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
Biophys J 94:4948-56. 2008..Given the high-throughput nature of the measurements, this deviation may be acceptable for many biological applications that seek to establish connections between morphology and function... - Molecular imaging of epidermal growth factor receptor in live cells with refractive index sensitivity using dark-field microspectroscopy and immunotargeted nanoparticlesAdam C Curry
Duke University, Department of Biomedical Engineering and Fitzpatrick Institute of Photonics, Durham, North Carolina 27708, USA
J Biomed Opt 13:014022. 2008..By demonstrating RI sensitivity over nanometer length scales, this study also presents a potential new avenue for assessing the structure and dynamics of live cells... - Label-free plasmonic detection of biomolecular binding by a single gold nanorodGreg J Nusz
Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA
Anal Chem 80:984-9. 2008..3 nm. Binding of streptavidin at 1 nM concentration induces a mean resonant wavelength shift of 0.59 nm suggesting that we are currently operating at close to the limit of detection of the system... - Polarization effects on scatterer sizing accuracy analyzed with frequency-domain angle-resolved low-coherence interferometryJohn W Pyhtila
Department of Biomedical Engineering, Duke University, North Carolina 27708, USA
Appl Opt 46:1735-41. 2007.... - Analysis of total uncertainty in spectral peak measurements for plasmonic nanoparticle-based biosensorsAdam Curry
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
Appl Opt 46:1931-9. 2007..We estimate thresholds of 310 streptavidin molecules for the presented scheme and 20 molecules with system improvements... - Application of Mie theory to determine the structure of spheroidal scatterers in biological materialsJustin D Keener
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
Opt Lett 32:1326-8. 2007.... - Temporal coherence and time-frequency distributions in spectroscopic optical coherence tomographyRobert N Graf
Department of Biomedical Engineering and the Fitzpatrick Institute for Photonics, Duke University, Durham, North Carolina 27708, USA
J Opt Soc Am A Opt Image Sci Vis 24:2186-95. 2007..The utility of time-frequency distributions is illustrated through analysis of calculated signals... - Fiber-optic interferometric two-dimensional scattering-measurement systemYizheng Zhu
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
Opt Lett 35:1641-3. 2010..Experimental data from samples with different sizes and acquired with different polarizations show excellent agreement with Mie theory, producing structural measurements with subwavelength accuracy... - In situ assessment of intraepithelial neoplasia in hamster trachea epithelium using angle-resolved low-coherence interferometryKevin J Chalut
Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
Cancer Epidemiol Biomarkers Prev 16:223-7. 2007.... - Separating the scattering and absorption coefficients using the real and imaginary parts of the refractive index with low-coherence interferometryFrancisco E Robles
Department of Biomedical Engineering and Medical Physics Program, Duke University, Durham, North Carolina 27708, USA
Opt Lett 35:2843-5. 2010..A derivation relating the real part of the RI to the nonlinear phase term of the signal is presented, along with measurements from scattering and nonscattering samples that exhibit absorption due to hemoglobin... - Multiplexed low coherence interferometry instrument for measuring microbicide gel thickness distributionTyler K Drake
Department of Medical Physics, Duke University, Durham, North Carolina 27708, USA
Appl Opt 48:D14-9. 2009..The potential impact of using a multiplexed LCI system for in vivo measurements is also discussed... - Size and shape determination of spheroidal scatterers using two-dimensional angle resolved scatteringMichael Giacomelli
Dept of Biomedical Engineering and Fitzpatrick Center for Photonics, Duke University, Durham, NC 27708, USA
Opt Express 18:14616-26. 2010..T-matrix based inverse analysis of these two-dimensional angular measurements yields completely unique size and aspect ratio determinations with subwavelength precision over a large range of possible scatterer geometries... - Nonlinear osmotic properties of the cell nucleusJohn D Finan
Department of Surgery, Duke University Medical Center, 375 MSRB, Box 3093, Durham, NC 27710, USA
Ann Biomed Eng 37:477-91. 2009..As nuclear morphology has been associated previously with cell phenotype, these findings may provide new insight into the role of mechanical and osmotic signals in regulating cell physiology...
Research Grants
- Assessing nuclear morphology in thick tissues using fLCIAdam Wax; Fiscal Year: 2007..3) Animal Studies: We will execute experiments with animal epithelial tissues which will demonstrate the ability of fLCI to detect nuclear morphology within intact samples and for detecting pre-cancerous changes. ..
- In vivo detection of pre-cancerous lesions using a/LCLAdam Wax; Fiscal Year: 2007..abstract_text> ..
- Assessing Deployment of Microbicidal Gels With Label-Free Optical MeasurementAdam Wax; Fiscal Year: 2007..The optical tool proposed here will allow the comparison of various formulations and deployment strategies of microbicidal gels as well as enabling assessment of the amount of time that a gel effectively coats the target surface. ..
- Coherence Imaging for Assessing Colorectal NeoplasiaAdam Wax; Fiscal Year: 2010..abstract_text> ..