DEBORAH STEEGE

Summary

Affiliation: Duke University Medical Center
Country: USA

Publications

  1. pmc Emerging features of mRNA decay in bacteria
    D A Steege
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 6:1079-90. 2000
  2. pmc Functional analysis of filamentous phage f1 mRNA processing sites
    M D Stump
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 2:1286-94. 1996
  3. pmc Appropriate expression of filamentous phage f1 DNA replication genes II and X requires RNase E-dependent processing and separate mRNAs
    R J Kokoska
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Bacteriol 180:3245-9. 1998
  4. pmc Roles of polyadenylation and nucleolytic cleavage in the filamentous phage mRNA processing and decay pathways in Escherichia coli
    A F Goodrich
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 5:972-85. 1999

Research Grants

Collaborators

  • A F Goodrich
  • R J Kokoska
  • M D Stump

Detail Information

Publications4

  1. pmc Emerging features of mRNA decay in bacteria
    D A Steege
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 6:1079-90. 2000
    ....
  2. pmc Functional analysis of filamentous phage f1 mRNA processing sites
    M D Stump
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 2:1286-94. 1996
    ..From their functional properties, we suggest that the known processing sites from phage f1 that are dependent on RNase E may be representative of relatively late steps in rne-dependent cleavage pathways...
  3. pmc Appropriate expression of filamentous phage f1 DNA replication genes II and X requires RNase E-dependent processing and separate mRNAs
    R J Kokoska
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Bacteriol 180:3245-9. 1998
    ..Whereas pII levels are limited at the level of mRNA stability, normal pX levels require transcription in sufficient amounts from the promoter for the smaller mRNA encoding only pX...
  4. pmc Roles of polyadenylation and nucleolytic cleavage in the filamentous phage mRNA processing and decay pathways in Escherichia coli
    A F Goodrich
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 5:972-85. 1999
    ..The results place polyadenylation at a later rather than an initiating step of decay. They also provide a clear illustration of how stably structured RNA 3' ends act as barriers to 3' --> 5' exonucleolytic mRNA decay...

Research Grants20

  1. NUCLEIC ACID STRUCTURE-FUNCTION IN GENE EXPRESSION
    DEBORAH STEEGE; Fiscal Year: 1991
    ....
  2. NUCLEIC ACID STRUCTURE-FUNCTION IN GENE EXPRESSION
    DEBORAH STEEGE; Fiscal Year: 1993
    ..As a conclusion to studies using cleavage-defective EcoRI endonuclease mutants as transcriptional roadblocks and probes of elongation complex structure and function, E.coli RNA polymerase movement along the DNA template will be examined...
  3. NUCLEIC ACID STRUCTURE/FUNCTION IN GENE EXPRESSION
    DEBORAH STEEGE; Fiscal Year: 2000
    ..The longterm rationale for this aim is to define as yet unidentified mRNA-rRNA interactions that mediate initiation complex assembly and thereby contribute to current efforts to refine the structure of 16S rRNA. ..
  4. NUCLEIC ACID STRUCTURE-FUNCTION IN GENE EXPRESSION
    DEBORAH STEEGE; Fiscal Year: 2005
    ..In a new direction, we will follow f1 mRNA decay and degradosome localization within the bacterial cell. ..