Melissa Skala

Summary

Affiliation: Duke University Medical Center
Country: USA

Publications

  1. pmc Photothermal optical coherence tomography of epidermal growth factor receptor in live cells using immunotargeted gold nanospheres
    Melissa C Skala
    Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
    Nano Lett 8:3461-7. 2008
  2. pmc Combined hyperspectral and spectral domain optical coherence tomography microscope for noninvasive hemodynamic imaging
    Melissa C Skala
    Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA
    Opt Lett 34:289-91. 2009
  3. pmc Multiphoton redox ratio imaging for metabolic monitoring in vivo
    Melissa Skala
    Department of Biomedical Engineering, Duke University, CIEMAS, Durham, NC, USA
    Methods Mol Biol 594:155-62. 2010
  4. pmc Longitudinal optical imaging of tumor metabolism and hemodynamics
    Melissa C Skala
    Duke University, Department of Biomedical Engineering, Durham, North Carolina 27708, USA
    J Biomed Opt 15:011112. 2010
  5. pmc In vivo multiphoton fluorescence lifetime imaging of protein-bound and free nicotinamide adenine dinucleotide in normal and precancerous epithelia
    Melissa C Skala
    Duke University, Department of Biomedical Engineering, Durham, North Carolina 27708, USA
    J Biomed Opt 12:024014. 2007
  6. pmc Investigation of fiber-optic probe designs for optical spectroscopic diagnosis of epithelial pre-cancers
    Melissa C Skala
    Department of Biomedical Engineering, University of Wisconsin, 1550 Engineering Drive, Madison, WI 53706, USA
    Lasers Surg Med 34:25-38. 2004
  7. pmc In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia
    Melissa C Skala
    Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA
    Proc Natl Acad Sci U S A 104:19494-9. 2007

Research Grants

Detail Information

Publications7

  1. pmc Photothermal optical coherence tomography of epidermal growth factor receptor in live cells using immunotargeted gold nanospheres
    Melissa C Skala
    Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
    Nano Lett 8:3461-7. 2008
    ..This technique could potentially augment confocal and multiphoton microscopy as a method for deep-tissue, depth-resolved molecular imaging with relatively high resolution and target sensitivity, without photobleaching or cytotoxicity...
  2. pmc Combined hyperspectral and spectral domain optical coherence tomography microscope for noninvasive hemodynamic imaging
    Melissa C Skala
    Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA
    Opt Lett 34:289-91. 2009
    ..There was a strong correlation between volumetric flow and hemoglobin saturation (rho=0.89, p=9x10(-6), N=15) and a moderate correlation between shear rate on the vessel wall and hemoglobin saturation (rho=0.56, p=0.03, N=15)...
  3. pmc Multiphoton redox ratio imaging for metabolic monitoring in vivo
    Melissa Skala
    Department of Biomedical Engineering, Duke University, CIEMAS, Durham, NC, USA
    Methods Mol Biol 594:155-62. 2010
    ..NADH and FAD are endogenous tissue fluorophores, and thus this method does not require exogenous stains or tissue excision. We describe the principles and protocols of multiphoton redox ratio imaging in vivo...
  4. pmc Longitudinal optical imaging of tumor metabolism and hemodynamics
    Melissa C Skala
    Duke University, Department of Biomedical Engineering, Durham, North Carolina 27708, USA
    J Biomed Opt 15:011112. 2010
    ....
  5. pmc In vivo multiphoton fluorescence lifetime imaging of protein-bound and free nicotinamide adenine dinucleotide in normal and precancerous epithelia
    Melissa C Skala
    Duke University, Department of Biomedical Engineering, Durham, North Carolina 27708, USA
    J Biomed Opt 12:024014. 2007
    ..We demonstrate that multiphoton FLIM is a powerful tool for the noninvasive characterization and detection of epithelial precancers in vivo...
  6. pmc Investigation of fiber-optic probe designs for optical spectroscopic diagnosis of epithelial pre-cancers
    Melissa C Skala
    Department of Biomedical Engineering, University of Wisconsin, 1550 Engineering Drive, Madison, WI 53706, USA
    Lasers Surg Med 34:25-38. 2004
    ..The second objective was to design a novel, angled illumination fiber-optic probe to maximally exploit the depth-dependent fluorescence properties of epithelial tissues...
  7. pmc In vivo multiphoton microscopy of NADH and FAD redox states, fluorescence lifetimes, and cellular morphology in precancerous epithelia
    Melissa C Skala
    Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA
    Proc Natl Acad Sci U S A 104:19494-9. 2007
    ..Increased intracellular variability in the redox ratio, NADH, and FAD fluorescence lifetimes were observed in precancerous cells compared with normal cells...

Research Grants2

  1. Molecular optical coherence tomography for monitoring cancer therapy
    Melissa Skala; Fiscal Year: 2007
    ..In the future, this technology could be used to provide individualized care to cancer patients, thus decreasing cancer morbidity and mortality. ..