S Shenolikar

Summary

Affiliation: Duke University Medical Center
Country: USA

Publications

  1. doi request reprint Urine electrolyte, mineral, and protein excretion in NHERF-2 and NHERF-1 null mice
    Rochelle Cunningham
    Univ of Maryland School of Medicine, 22 S Greene St, N3W143, Baltimore, MD 21201, USA
    Am J Physiol Renal Physiol 294:F1001-7. 2008
  2. ncbi request reprint N-terminal PDZ domain is required for NHERF dimerization
    S Shenolikar
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    FEBS Lett 489:233-6. 2001
  3. ncbi request reprint NHERF: targeting and trafficking membrane proteins
    S Shenolikar
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Am J Physiol Renal Physiol 280:F389-95. 2001
  4. ncbi request reprint Meeting report: targeting protein phosphatases-medicines for the new millenium
    S Shenolikar
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC, USA
    Sci STKE 2000:pe1. 2000
  5. pmc Targeted disruption of the mouse NHERF-1 gene promotes internalization of proximal tubule sodium-phosphate cotransporter type IIa and renal phosphate wasting
    S Shenolikar
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 99:11470-5. 2002
  6. pmc Growth arrest and DNA damage-inducible protein GADD34 assembles a novel signaling complex containing protein phosphatase 1 and inhibitor 1
    J H Connor
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Cell Biol 21:6841-50. 2001
  7. ncbi request reprint Expanding the role of NHERF, a PDZ-domain containing protein adapter, to growth regulation
    J W Voltz
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, NC 27710 USA
    Oncogene 20:6309-14. 2001
  8. ncbi request reprint The beta2-adrenergic receptor interacts with the Na+/H+-exchanger regulatory factor to control Na+/H+ exchange
    R A Hall
    Howard Hughes Medical Institute, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nature 392:626-30. 1998
  9. ncbi request reprint Multiple structural elements define the specificity of recombinant human inhibitor-1 as a protein phosphatase-1 inhibitor
    S Endo
    Department of Pharmacology, Duke University Medical Center, Durham, North Carolina 27708, USA
    Biochemistry 35:5220-8. 1996
  10. ncbi request reprint Detection of phosphorylation by enzymatic techniques
    S Shenolikar
    Duke University Medical Center, Durham, North Carolina, USA
    Curr Protoc Protein Sci . 2001

Research Grants

  1. PHOSPHATASE INHIBITOR 1, AN AMPLIFIER OF CAMP SIGNALS
    Shirish Shenolikar; Fiscal Year: 2001
  2. Protein Phosphatase-1-Targeting the Cytoskeleton
    Shirish Shenolikar; Fiscal Year: 2005
  3. Phosphatase Inhibitor-1, An Amplifier of cAMP Signals
    Shirish Shenolikar; Fiscal Year: 2005

Collaborators

Detail Information

Publications31

  1. doi request reprint Urine electrolyte, mineral, and protein excretion in NHERF-2 and NHERF-1 null mice
    Rochelle Cunningham
    Univ of Maryland School of Medicine, 22 S Greene St, N3W143, Baltimore, MD 21201, USA
    Am J Physiol Renal Physiol 294:F1001-7. 2008
    ....
  2. ncbi request reprint N-terminal PDZ domain is required for NHERF dimerization
    S Shenolikar
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    FEBS Lett 489:233-6. 2001
    ..In PS120 cells, reversible protein phosphorylation modulated NHERF dimerization and suggested a role for NHERF dimers in hormonal signaling...
  3. ncbi request reprint NHERF: targeting and trafficking membrane proteins
    S Shenolikar
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Am J Physiol Renal Physiol 280:F389-95. 2001
    ..This article reviews the new evidence that implicates NHERF in wider aspects of epithelial membrane biology...
  4. ncbi request reprint Meeting report: targeting protein phosphatases-medicines for the new millenium
    S Shenolikar
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC, USA
    Sci STKE 2000:pe1. 2000
    ..A second important issue presented related to identification and characterization of various phosphatase-binding proteins that regulate phosphatase action...
  5. pmc Targeted disruption of the mouse NHERF-1 gene promotes internalization of proximal tubule sodium-phosphate cotransporter type IIa and renal phosphate wasting
    S Shenolikar
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 99:11470-5. 2002
    ..Phosphate wasting seen in the NHERF-1(-/-) null mice provided a new experimental system for defining the role of PDZ adapters in the hormonal control of ion transport and renal disease...
  6. pmc Growth arrest and DNA damage-inducible protein GADD34 assembles a novel signaling complex containing protein phosphatase 1 and inhibitor 1
    J H Connor
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Cell Biol 21:6841-50. 2001
    ..These studies suggested that modification of the I-1/GADD34/PP1 signaling complex regulates the initiation of protein translation in mammalian tissues...
  7. ncbi request reprint Expanding the role of NHERF, a PDZ-domain containing protein adapter, to growth regulation
    J W Voltz
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, NC 27710 USA
    Oncogene 20:6309-14. 2001
    ....
  8. ncbi request reprint The beta2-adrenergic receptor interacts with the Na+/H+-exchanger regulatory factor to control Na+/H+ exchange
    R A Hall
    Howard Hughes Medical Institute, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nature 392:626-30. 1998
    ..Our findings indicate that agonist-dependent beta2-adrenergic receptor binding of NHERF plays a role in beta2-adrenergic receptor-mediated regulation of Na+/H+ exchange...
  9. ncbi request reprint Multiple structural elements define the specificity of recombinant human inhibitor-1 as a protein phosphatase-1 inhibitor
    S Endo
    Department of Pharmacology, Duke University Medical Center, Durham, North Carolina 27708, USA
    Biochemistry 35:5220-8. 1996
    ..Our data point to multiple interactions between the I-1 functional domain. and the PP1 catalytic subunit that define this phosphoprotein as a physiological regulator of the type-1 protein phosphatase...
  10. ncbi request reprint Detection of phosphorylation by enzymatic techniques
    S Shenolikar
    Duke University Medical Center, Durham, North Carolina, USA
    Curr Protoc Protein Sci . 2001
    ..A support protocol has been included to identify the radiolabel as (32)P(I) based on its ability to form a complex with ammonium molybdate...
  11. ncbi request reprint Detection of phosphorylation by enzymatic techniques
    S Shenolikar
    Duke University Medical Center, Durham, North Carolina, USA
    Curr Protoc Mol Biol . 2001
    ..A support protocol has been included to identify the radiolabel as (32)Pi based on its ability to form a complex with ammonium molybdate...
  12. ncbi request reprint Cystic fibrosis transmembrane conductance regulator activation is reduced in the small intestine of Na+/H+ exchanger 3 regulatory factor 1 (NHERF-1)- but Not NHERF-2-deficient mice
    Nellie Broere
    Department of Biochemistry, Erasmus University Medical Center, 3015 GE, Rotterdam, The Netherlands
    J Biol Chem 282:37575-84. 2007
    ....
  13. ncbi request reprint Phosphorylation of PDZ1 domain attenuates NHERF-1 binding to cellular targets
    James W Voltz
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 282:33879-87. 2007
    ....
  14. pmc Parathyroid hormone inhibits renal phosphate transport by phosphorylation of serine 77 of sodium-hydrogen exchanger regulatory factor-1
    Edward J Weinman
    Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Clin Invest 117:3412-20. 2007
    ..These results indicate that PTH-mediated inhibition of renal phosphate transport involves phosphorylation of S77 of the NHERF-1 PDZ I domain and the dissociation of NHERF-1/Npt2a complexes...
  15. ncbi request reprint Sodium-hydrogen exchanger regulatory factor-1 interacts with mouse urate transporter 1 to regulate renal proximal tubule uric acid transport
    Rochelle Cunningham
    Department of Medicine, University of Maryland School of Medicine, 22 S Greene St, N3W143, Baltimore, MD 21201, USA
    J Am Soc Nephrol 18:1419-25. 2007
    ..These findings indicate that NHERF-1 exerts a significant effect on the renal tubular reabsorption of uric acid in the mouse by modulating the BBM abundance of mURAT1 and possibly other BBM uric acid transporters...
  16. ncbi request reprint Defective coupling of apical PTH receptors to phospholipase C prevents internalization of the Na+-phosphate cotransporter NaPi-IIa in Nherf1-deficient mice
    Paola Capuano
    Institute of Physiology, Univ of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Am J Physiol Cell Physiol 292:C927-34. 2007
    ..These data suggest that NHERF1 in the proximal tubule is important for PTH-induced internalization of NaP(i)-IIa and, specifically, couples the apical PTHR to PLC...
  17. ncbi request reprint Adenoviral expression of NHERF-1 in NHERF-1 null mouse renal proximal tubule cells restores Npt2a regulation by low phosphate media and parathyroid hormone
    Rochelle Cunningham
    Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Am J Physiol Renal Physiol 291:F896-901. 2006
    ..Together, these results highlight the requirement for NHERF-1 for physiological control of Npt2a trafficking and suggest that the Npt2a/NHERF-1 complex represents a unique PTH-responsive pool of Npt2a in renal microvilli...
  18. ncbi request reprint Longitudinal study of urinary excretion of phosphate, calcium, and uric acid in mutant NHERF-1 null mice
    Edward J Weinman
    Department of Medicine, University of Maryland School of Medicine, Baltimore 21202, USA
    Am J Physiol Renal Physiol 290:F838-43. 2006
    ..These studies indicate defects in the renal tubule transport of phosphate, calcium, and uric acid in NHERF-1(-/-) male and female mice that could account for the increased deposition of calcium in the papilla of null mice...
  19. ncbi request reprint Defective PTH regulation of sodium-dependent phosphate transport in NHERF-1-/- renal proximal tubule cells and wild-type cells adapted to low-phosphate media
    Rochelle Cunningham
    Department of Medicine, Department of Veterans Affairs Medical Center, Baltimore, Maryland, USA
    Am J Physiol Renal Physiol 289:F933-8. 2005
    ..These studies also suggest that there is a blunted phosphaturic effect of PTH in cells adapted to low-phosphate media...
  20. pmc The role of NHERF-1 in the regulation of renal proximal tubule sodium-hydrogen exchanger 3 and sodium-dependent phosphate cotransporter 2a
    Edward J Weinman
    Department of Medicine, Division of Nephrology, University of Maryland, School of Medicine, 22, South Greene Street, Baltimore, MD 21202, USA
    J Physiol 567:27-32. 2005
    ..These studies indicate a unique requirement for NHERF-1 in cAMP regulation of NHE3 and in the trafficking of Npt2a...
  21. ncbi request reprint NHERF and regulation of the renal sodium-hydrogen exchanger NHE3
    Edward J Weinman
    Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Pflugers Arch 450:137-44. 2005
    ..In this review, we trace the discovery of NHERF-1 and NHERF-2, and update our current understanding of the relation between these proteins and the regulation and trafficking of NHE3...
  22. ncbi request reprint Defective parathyroid hormone regulation of NHE3 activity and phosphate adaptation in cultured NHERF-1-/- renal proximal tubule cells
    Rochelle Cunningham
    Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 279:37815-21. 2004
    ..NHERF-1(-/-) cells also do not adapt to alterations in the phosphate content of the media indicating that the defect resides within the cells of the proximal tubule and is not dependent on systemic factors...
  23. ncbi request reprint A C-terminal PDZ motif in NHE3 binds NHERF-1 and enhances cAMP inhibition of sodium-hydrogen exchange
    Edward J Weinman
    Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Biochemistry 42:12662-8. 2003
    ..Together, the data suggested that the PDZ interaction between the NHE3 C-terminus and a NHERF-1 PDZ domain enhanced the regulation of sodium-hydrogen exchange by cAMP-elevating hormones...
  24. ncbi request reprint NHERF-1 is required for renal adaptation to a low-phosphate diet
    Edward J Weinman
    Department of Medicine, Division of Nephrology, University of Maryland School of Medicine, Rm N3W143, UHM, 22 South Greene Street, Baltimore, MD 21201, USA
    Am J Physiol Renal Physiol 285:F1225-32. 2003
    ..These experiments demonstrate that full renal adaptation to a low-phosphate diet requires NHERF-1, which serves to increase BBM expression of Npt2...
  25. ncbi request reprint Localization and interaction of NHERF isoforms in the renal proximal tubule of the mouse
    James B Wade
    Dept of Physiology, 655 W, Baltimore St, Univ of Maryland, Baltimore, MD 21201, USA
    Am J Physiol Cell Physiol 285:C1494-503. 2003
    ..These findings suggest that the organized subcellular distribution of the NHERF isoforms may play a role in the specific interactions mediating physiological control of transporter function...
  26. ncbi request reprint NHERF-1 uniquely transduces the cAMP signals that inhibit sodium-hydrogen exchange in mouse renal apical membranes
    Edward J Weinman
    Department of Medicine, Division Nephrology, University of Maryland School of Medicine, Room N3W143, UHM, 22 South Greene Street, Baltimore, MD 21201, USA
    FEBS Lett 536:141-4. 2003
    ..As the protein levels for NHE3, NHERF-2, PKA and ezrin were not changed in the NHERF-1 (-/-) BBMs, the data suggest a unique role for NHERF-1 in cAMP-mediated inhibition of NHE3 activity in the renal proximal tubule of the mouse...
  27. ncbi request reprint Neurabins recruit protein phosphatase-1 and inhibitor-2 to the actin cytoskeleton
    Ryan T Terry-Lorenzo
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:46535-43. 2002
    ..I-2 complex to actin cytoskeleton and regulate cell morphology...
  28. ncbi request reprint Expression of human protein phosphatase-1 in Saccharomyces cerevisiae highlights the role of phosphatase isoforms in regulating eukaryotic functions
    Jennifer A Gibbons
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA
    J Biol Chem 282:21838-47. 2007
    ..Thus, PP1 expression in yeast should facilitate future structure-function studies of this protein serine/threonine phosphatase...
  29. ncbi request reprint The association of NHERF adaptor proteins with g protein-coupled receptors and receptor tyrosine kinases
    Edward J Weinman
    Department of Medicine, University of Maryland School of Medicine, and Medical Service, Department of Veterans Affairs Medical Center, Baltimore, Maryland 21201, USA
    Annu Rev Physiol 68:491-505. 2006
    ..This review summarizes the emerging data on the biochemical mechanisms, physiologic outcomes, and potential clinical implications of the assembly and disassembly of receptor/NHERF complexes...
  30. pmc Targeting protein phosphatase 1 (PP1) to the actin cytoskeleton: the neurabin I/PP1 complex regulates cell morphology
    Carey J Oliver
    Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Cell Biol 22:4690-701. 2002
    ..These studies provided new insights into the assembly and regulation of a neurabin I/PP1 complex that controls actin rearrangement to promote spine development in mammalian neurons...
  31. ncbi request reprint Regulation of ion transport by the NHERF family of PDZ proteins
    Shirish Shenolikar
    Department Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Physiology (Bethesda) 19:362-9. 2004
    ....

Research Grants14

  1. PHOSPHATASE INHIBITOR 1, AN AMPLIFIER OF CAMP SIGNALS
    Shirish Shenolikar; Fiscal Year: 2001
    ..These studies are relevant to targeting phosphatases to modulate hormonal responses and ameliorate metabolic dysfunctions in many human diseases. ..
  2. Protein Phosphatase-1-Targeting the Cytoskeleton
    Shirish Shenolikar; Fiscal Year: 2005
    ..Elucidating neurabin-mediated PP1 signaling may define the defects in plasticity that contribute to mental retardation, epilepsy and neurodegenerative disease. ..
  3. Phosphatase Inhibitor-1, An Amplifier of cAMP Signals
    Shirish Shenolikar; Fiscal Year: 2005
    ..2) Define the role of GADD34 in the assembly of the mammalian eIF2alpha phosphatase and the transduction of physiological signals that regulate protein synthesis and dictate the switch between cell death and survival. ..