Sudarshan Rajagopal

Summary

Affiliation: Duke University Medical Center
Country: USA

Publications

  1. pmc Total chemical synthesis and biophysical characterization of the minimal isoform of the KChIP2 potassium channel regulatory subunit
    Sudarshan Rajagopal
    Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois 60637, USA
    Protein Sci 16:2056-64. 2007
  2. ncbi request reprint A structural pathway for signaling in the E46Q mutant of photoactive yellow protein
    Sudarshan Rajagopal
    Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637, USA
    Structure 13:55-63. 2005
  3. ncbi request reprint Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein
    Spencer Anderson
    Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA
    Structure 12:1039-45. 2004
  4. ncbi request reprint Analysis of experimental time-resolved crystallographic data by singular value decomposition
    Sudarshan Rajagopal
    Department of Biochemistry and Molecular Biology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Acta Crystallogr D Biol Crystallogr 60:860-71. 2004
  5. ncbi request reprint Analytical trapping: extraction of time-independent structures from time-dependent crystallographic data
    Sudarshan Rajagopal
    Department of Biochemistry and Molecular Biology, The University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    J Struct Biol 147:211-22. 2004
  6. ncbi request reprint Purification and initial characterization of a putative blue light-regulated phosphodiesterase from Escherichia coli
    Sudarshan Rajagopal
    Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA
    Photochem Photobiol 80:542-7. 2004
  7. pmc Crystal structure of a photoactive yellow protein from a sensor histidine kinase: conformational variability and signal transduction
    Sudarshan Rajagopal
    Department of Biochemistry and Molecular Biology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 100:1649-54. 2003
  8. ncbi request reprint The LOV domain family: photoresponsive signaling modules coupled to diverse output domains
    Sean Crosson
    Department of Biochemistry and Molecular Biology, University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA
    Biochemistry 42:2-10. 2003
  9. pmc Protein kinetics: structures of intermediates and reaction mechanism from time-resolved x-ray data
    Marius Schmidt
    Physikdepartment E17, Technische Universitat Munchen, 85747 Garching, Germany
    Proc Natl Acad Sci U S A 101:4799-804. 2004
  10. pmc Application of singular value decomposition to the analysis of time-resolved macromolecular x-ray data
    Marius Schmidt
    Physik Department E17, Technische Universitaet Muenchen, 85747 Garching, Germany
    Biophys J 84:2112-29. 2003

Collaborators

Detail Information

Publications11

  1. pmc Total chemical synthesis and biophysical characterization of the minimal isoform of the KChIP2 potassium channel regulatory subunit
    Sudarshan Rajagopal
    Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois 60637, USA
    Protein Sci 16:2056-64. 2007
    ..2. Planned future studies include the incorporation of fluorescent and spin labeled probes in KChIP2d to yield structural information in parallel with electrophysiologic studies to elucidate KChIP2d's mechanism of action...
  2. ncbi request reprint A structural pathway for signaling in the E46Q mutant of photoactive yellow protein
    Sudarshan Rajagopal
    Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637, USA
    Structure 13:55-63. 2005
    ....
  3. ncbi request reprint Chromophore conformation and the evolution of tertiary structural changes in photoactive yellow protein
    Spencer Anderson
    Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA
    Structure 12:1039-45. 2004
    ....
  4. ncbi request reprint Analysis of experimental time-resolved crystallographic data by singular value decomposition
    Sudarshan Rajagopal
    Department of Biochemistry and Molecular Biology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Acta Crystallogr D Biol Crystallogr 60:860-71. 2004
    ..Procedures for the effective SVD analysis of experimental time-resolved crystallographic data have been established and emphasize the necessity for minimizing systematic errors by modification of the data-collection protocol...
  5. ncbi request reprint Analytical trapping: extraction of time-independent structures from time-dependent crystallographic data
    Sudarshan Rajagopal
    Department of Biochemistry and Molecular Biology, The University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    J Struct Biol 147:211-22. 2004
    ..The strategies are illustrated by using data on the photocycle of the bacterial blue light photoreceptor, photoactive yellow protein...
  6. ncbi request reprint Purification and initial characterization of a putative blue light-regulated phosphodiesterase from Escherichia coli
    Sudarshan Rajagopal
    Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL 60637, USA
    Photochem Photobiol 80:542-7. 2004
    ..Predicted proteins from Klebsiella pneumoniae and from a bacterium in the Sargasso Sea are similar to E. coli Blrp in both their BLUF and EAL domains, which suggests that blue light sensing in these bacteria may follow similar pathways...
  7. pmc Crystal structure of a photoactive yellow protein from a sensor histidine kinase: conformational variability and signal transduction
    Sudarshan Rajagopal
    Department of Biochemistry and Molecular Biology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 100:1649-54. 2003
    ..Because of its direct linkage to a measurable biological output, Ppr-PYP serves as an excellent system for understanding how changes in photocycle dynamics affect signaling by PYPs...
  8. ncbi request reprint The LOV domain family: photoresponsive signaling modules coupled to diverse output domains
    Sean Crosson
    Department of Biochemistry and Molecular Biology, University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA
    Biochemistry 42:2-10. 2003
    ..We discuss the role of this conserved volume of structure in LOV-regulated processes...
  9. pmc Protein kinetics: structures of intermediates and reaction mechanism from time-resolved x-ray data
    Marius Schmidt
    Physikdepartment E17, Technische Universitat Munchen, 85747 Garching, Germany
    Proc Natl Acad Sci U S A 101:4799-804. 2004
    ..Stoichiometric and structural constraints allow the exclusion of one kinetic mechanism proposed for the photocycle but retain other plausible candidate kinetic mechanisms...
  10. pmc Application of singular value decomposition to the analysis of time-resolved macromolecular x-ray data
    Marius Schmidt
    Physik Department E17, Technische Universitaet Muenchen, 85747 Garching, Germany
    Biophys J 84:2112-29. 2003
    ..This demonstrates that SVD will be a powerful tool in the analysis of experimental time-resolved crystallographic data...
  11. pmc Visualizing reaction pathways in photoactive yellow protein from nanoseconds to seconds
    Hyotcherl Ihee
    Department of Chemistry and School of Molecular Science BK21, Korea Advanced Institute of Science and Technology KAIST, Daejeon 305 701, South Korea
    Proc Natl Acad Sci U S A 102:7145-50. 2005
    ..These results yield a comprehensive view of the PYP photocycle when seen in the light of previous biophysical studies on the system...