Richard Premont

Summary

Affiliation: Duke University Medical Center
Country: USA

Publications

  1. ncbi request reprint The GIT family of ADP-ribosylation factor GTPase-activating proteins. Functional diversity of GIT2 through alternative splicing
    R T Premont
    Departments of Medicine and Biochemistry, Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 275:22373-80. 2000
  2. ncbi request reprint Physiological roles of G protein-coupled receptor kinases and arrestins
    Richard T Premont
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Annu Rev Physiol 69:511-34. 2007
  3. ncbi request reprint Once and future signaling: G protein-coupled receptor kinase control of neuronal sensitivity
    Richard T Premont
    Liver Center, Division of Gastroenterology, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Neuromolecular Med 7:129-47. 2005
  4. doi request reprint Metastasis: wherefore arf thou?
    Richard T Premont
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 19:R1036-8. 2009
  5. ncbi request reprint ACAP1 promotes endocytic recycling by recognizing recycling sorting signals
    Jun Dai
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 7:771-6. 2004
  6. ncbi request reprint Mutational analysis of the Arf1*GTP/Arf GAP interface reveals an Arf1 mutant that selectively affects the Arf GAP ASAP1
    RuiBai Luo
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Curr Biol 15:2164-9. 2005
  7. ncbi request reprint beta-arrestin-dependent, G protein-independent ERK1/2 activation by the beta2 adrenergic receptor
    Sudha K Shenoy
    Howard Hughes Medical Institute at Duke University Medical Center, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    J Biol Chem 281:1261-73. 2006
  8. ncbi request reprint Vasoactive intestinal polypeptide type-1 receptor regulation. Desensitization, phosphorylation, and sequestration
    Michael A Shetzline
    Department of Cell Biology, Division of Gastroenterology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:25519-26. 2002
  9. ncbi request reprint G protein-coupled receptor kinase 5 regulates beta 1-adrenergic receptor association with PSD-95
    Liaoyuan A Hu
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:1607-13. 2002
  10. pmc ARFGAP1 plays a central role in coupling COPI cargo sorting with vesicle formation
    Stella Y Lee
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, and Department of Medicine, Harvard Medical School, Boston, MA 02115
    J Cell Biol 168:281-90. 2005

Research Grants

  1. Dopamine Receptor Regulation by GRKs in Drug Abuse
    Richard Premont; Fiscal Year: 2007
  2. SIGNALING PATHWAYS OF ARF GTPASES AND ARF GAPS
    Richard Premont; Fiscal Year: 2005

Collaborators

Detail Information

Publications33

  1. ncbi request reprint The GIT family of ADP-ribosylation factor GTPase-activating proteins. Functional diversity of GIT2 through alternative splicing
    R T Premont
    Departments of Medicine and Biochemistry, Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 275:22373-80. 2000
    ..Although GIT2 shares many properties with GIT1, it also exhibits both structural and functional diversity due to tissue-specific alternative splicing...
  2. ncbi request reprint Physiological roles of G protein-coupled receptor kinases and arrestins
    Richard T Premont
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Annu Rev Physiol 69:511-34. 2007
    ....
  3. ncbi request reprint Once and future signaling: G protein-coupled receptor kinase control of neuronal sensitivity
    Richard T Premont
    Liver Center, Division of Gastroenterology, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Neuromolecular Med 7:129-47. 2005
    ..An understanding of this regulatory specificity could allow targeting of GRK function to ameliorate diseases involving GPCR dysregulation...
  4. doi request reprint Metastasis: wherefore arf thou?
    Richard T Premont
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Curr Biol 19:R1036-8. 2009
    ..A recent study identifies yet another role for Arf6 in metastasis - as a regulator of plasma-membrane-derived microvesicle release...
  5. ncbi request reprint ACAP1 promotes endocytic recycling by recognizing recycling sorting signals
    Jun Dai
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 7:771-6. 2004
    ..Taken together, these findings indicate that ACAP1 promotes cargo sorting by recognizing recycling sorting signals...
  6. ncbi request reprint Mutational analysis of the Arf1*GTP/Arf GAP interface reveals an Arf1 mutant that selectively affects the Arf GAP ASAP1
    RuiBai Luo
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Curr Biol 15:2164-9. 2005
    ..Based on our results, we conclude that the contribution of specific residues within switch 1 of Arf to binding and achieving a transition state toward GTP hydrolysis differs among Arf GAPs...
  7. ncbi request reprint beta-arrestin-dependent, G protein-independent ERK1/2 activation by the beta2 adrenergic receptor
    Sudha K Shenoy
    Howard Hughes Medical Institute at Duke University Medical Center, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    J Biol Chem 281:1261-73. 2006
    ..These findings demonstrate that the beta2AR can signal to ERK via a GRK5/6-beta-arrestin-dependent pathway, which is independent of G protein coupling...
  8. ncbi request reprint Vasoactive intestinal polypeptide type-1 receptor regulation. Desensitization, phosphorylation, and sequestration
    Michael A Shetzline
    Department of Cell Biology, Division of Gastroenterology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:25519-26. 2002
    ..Moreover, study of endogenously expressed receptors may provide information not evident in overexpressed systems...
  9. ncbi request reprint G protein-coupled receptor kinase 5 regulates beta 1-adrenergic receptor association with PSD-95
    Liaoyuan A Hu
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:1607-13. 2002
    ..Regulation of protein association through receptor phosphorylation may be a general mechanism used by G protein-coupled receptors that associate via PDZ domain-mediated protein/protein interactions...
  10. pmc ARFGAP1 plays a central role in coupling COPI cargo sorting with vesicle formation
    Stella Y Lee
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, and Department of Medicine, Harvard Medical School, Boston, MA 02115
    J Cell Biol 168:281-90. 2005
    ..Together, these findings indicate that GAP plays a central role in coupling cargo sorting and vesicle formation, with implications for simplifying models to describe how these two processes are coupled during COPI transport...
  11. pmc GIT1 utilizes a focal adhesion targeting-homology domain to bind paxillin
    Robert Schmalzigaug
    Division of Gastroenterology, Department of Medicine, Duke University Medical Center, Durham, NC 27710, United States
    Cell Signal 19:1733-44. 2007
    ..Thus, despite apparent structural similarity in their FAT domains, GIT1 and FAK binding to paxillin is differentially regulated...
  12. ncbi request reprint Desensitization of G protein-coupled receptors and neuronal functions
    Raul R Gainetdinov
    Howard Hughes Medical Institute Laboratories, Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Annu Rev Neurosci 27:107-44. 2004
    ..Furthermore, the utility of a pharmacological strategy aimed at targeting this GPCR desensitization machinery to regulate brain functions can be envisaged...
  13. ncbi request reprint Differences between AGAP1, ASAP1 and Arf GAP1 in substrate recognition: interaction with the N-terminus of Arf1
    Hye Young Yoon
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Building 37, Room 4118, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Signal 16:1033-44. 2004
    ..Taken together, our results indicate that (i) Arf GAPs interact with amino acids 2-17 of Arf1 and (ii) each subgroup of Arf GAPs has a unique interface with Arf1...
  14. ncbi request reprint The GIT/PIX complex: an oligomeric assembly of GIT family ARF GTPase-activating proteins and PIX family Rac1/Cdc42 guanine nucleotide exchange factors
    Richard T Premont
    Departments of Medicine and Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    Cell Signal 16:1001-11. 2004
    ..Thus, GIT and PIX proteins are tightly associated as a multimeric nexus capable of linking together important signaling molecules, including PAKs...
  15. ncbi request reprint Mammalian Scribble forms a tight complex with the betaPIX exchange factor
    Stéphane Audebert
    Molecular Pharmacology, Institut de Recherches sur le Cancer de Marseille, Unite mixte de recherche 599 Inserm Institut Paoli Calmettes, 27 Boulevard Lei Roure, 13009 Marseille, France
    Curr Biol 14:987-95. 2004
    ..These data reveal the existence of a tight hScrib/betaPIX interaction and suggest that this complex potentially plays a role in neuronal transmission...
  16. ncbi request reprint The GIT family of proteins forms multimers and associates with the presynaptic cytomatrix protein Piccolo
    Seho Kim
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Korea
    J Biol Chem 278:6291-300. 2003
    ..These results suggest that, through their multimerization and interaction with Piccolo, the GIT family proteins are involved in the organization of the CAZ...
  17. ncbi request reprint Interaction between liprin-alpha and GIT1 is required for AMPA receptor targeting
    Jaewon Ko
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Korea
    J Neurosci 23:1667-77. 2003
    ..These results suggest that the GIT1-liprin-alpha interaction is required for AMPA receptor targeting and that GIT1 may play an important role in the organization of presynaptic and postsynaptic multiprotein complexes...
  18. ncbi request reprint Dopaminergic supersensitivity in G protein-coupled receptor kinase 6-deficient mice
    Raul R Gainetdinov
    Howard Hughes Medical Institute Laboratories, Duke University Medical Center, Durham, NC 27710, USA
    Neuron 38:291-303. 2003
    ..The present study indicates that postsynaptic D2-like dopamine receptors are physiological targets for GRK6 and suggests that this regulatory mechanism contributes to central dopaminergic supersensitivity...
  19. ncbi request reprint GIPC interacts with the beta1-adrenergic receptor and regulates beta1-adrenergic receptor-mediated ERK activation
    Liaoyuan A Hu
    Howard Hughes Medical Institute, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 278:26295-301. 2003
    ..These data suggest that GIPC can regulate beta1-adrenergic receptor-stimulated, Gi-mediated, ERK activation while having no effect on receptor internalization or Gs-mediated cAMP signaling...
  20. ncbi request reprint Endothelin-1 activates endothelial cell nitric-oxide synthase via heterotrimeric G-protein betagamma subunit signaling to protein jinase B/Akt
    Songling Liu
    Duke University Liver Center, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 278:49929-35. 2003
    ..Taken together, the data elucidate a G-protein-coupled receptor signaling pathway for ETB receptor-mediated NO production and call attention to the absolute requirement for heterotrimeric G-protein betagamma subunits in this cascade...
  21. pmc Consensus nomenclature for the human ArfGAP domain-containing proteins
    Richard A Kahn
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    J Cell Biol 182:1039-44. 2008
    ..This article describes the resulting consensus nomenclature and provides a brief description of each of the 10 subfamilies of 31 human genes encoding proteins containing the ArfGAP domain...
  22. ncbi request reprint Differential expression of the ARF GAP genes GIT1 and GIT2 in mouse tissues
    Robert Schmalzigaug
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    J Histochem Cytochem 55:1039-48. 2007
    ....
  23. ncbi request reprint Regulation of neuroendocrine exocytosis by the ARF6 GTPase-activating protein GIT1
    Maria Zeniou Meyer
    Department of Neurotransmission and Neuroendocrine Secretion, Institut des Neurosciences Cellulaires et Intégratives UMR 7168 CNRS Université Louis Pasteur, 5 rue Blaise Pascal, 67084 Strasbourg, France
    J Biol Chem 281:7919-26. 2006
    ..We propose that GIT1 represents part of the pathway that inactivates ARF6-dependent reactions and thereby negatively regulates and/or terminates exocytotic release...
  24. pmc G protein-coupled receptor kinase 6 controls chronicity and severity of dextran sodium sulphate-induced colitis in mice
    Niels Eijkelkamp
    Laboratory for Psychoneuroimmunology, University Medical Center Utrecht, Lundlaan 6, Room KC 03 063 0, 3584 EA Utrecht, The Netherlands
    Gut 56:847-54. 2007
    ..Therefore, it is hypothesised that GRK6 plays a role in determining the course of inflammation...
  25. pmc Beta-arrestin2-mediated inotropic effects of the angiotensin II type 1A receptor in isolated cardiac myocytes
    Keshava Rajagopal
    Department of Surgery, Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 103:16284-9. 2006
    ..Such ligands may have potential as a novel class of therapeutic agents...
  26. ncbi request reprint Regulation of the platelet-derived growth factor receptor-beta by G protein-coupled receptor kinase-5 in vascular smooth muscle cells involves the phosphatase Shp2
    Jiao Hui Wu
    Department of Medicine Cardiology, Duke University, Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 281:37758-72. 2006
    ....
  27. ncbi request reprint A crucial role for GRK2 in regulation of endothelial cell nitric oxide synthase function in portal hypertension
    Songling Liu
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nat Med 11:952-8. 2005
    ..Thus, an important mechanism underlying impaired activity of eNOS in injured sinusoidal endothelial cells is defective phosphorylation of Akt caused by overexpression of GRK2 after injury...
  28. ncbi request reprint The Arf GAPs AGAP1 and AGAP2 distinguish between the adaptor protein complexes AP-1 and AP-3
    Zhongzhen Nie
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Sci 118:3555-66. 2005
    ..Based on these results, we concluded that the closely related Arf GAPs, AGAP1 and AGAP2, distinguish between these related heterotetrameric adaptor protein complexes to specifically regulate AP-3 endosomes and AP-1 recycling endosomes...
  29. pmc Dopamine supersensitivity correlates with D2High states, implying many paths to psychosis
    Philip Seeman
    Pharmacology Department, Medical Science Building, Room 4344, University of Toronto, 1 King s College Circle, Toronto, ON, Canada M5S 1A8
    Proc Natl Acad Sci U S A 102:3513-8. 2005
    ..If many types of brain impairment cause dopamine behavioral supersensitivity and a common increase in D2High states, it suggests that there are many pathways to psychosis, any one of which can be disrupted...
  30. pmc Defective lymphocyte chemotaxis in beta-arrestin2- and GRK6-deficient mice
    Alan M Fong
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 99:7478-83. 2002
    ..Thus, these results indicate that beta-arrestin2 and GRK6 actually play positive regulatory roles in mediating the chemotactic responses of T and B lymphocytes to CXCL12...
  31. ncbi request reprint GRK6 deficiency is associated with enhanced CXCR4-mediated neutrophil chemotaxis in vitro and impaired responsiveness to G-CSF in vivo
    Anne Vroon
    Laboratory of Psychoneuroimmunology, University Medical Center Utrecht, The Netherlands
    J Leukoc Biol 75:698-704. 2004
    ..In view of these data, we suggest that the impaired G-CSF-induced neutrophil mobilization in the absence of GRK6 may be a result of enhanced CXCR4-mediated retention of PMN in the bone marrow...
  32. ncbi request reprint Increased acute inflammation, leukotriene B4-induced chemotaxis, and signaling in mice deficient for G protein-coupled receptor kinase 6
    Annemieke Kavelaars
    Laboratory for Psychoneuroimmunology, University Medical Center Utrecht, Utrecht, The Netherlands
    J Immunol 171:6128-34. 2003
    ..In conclusion, GRK6 deficiency leads to prolonged BLT1 signaling and increased neutrophil migration...
  33. pmc ARFGAP1 promotes the formation of COPI vesicles, suggesting function as a component of the coat
    Jia Shu Yang
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 159:69-78. 2002
    ..Collectively, these findings suggest that ARFGAP1 promotes vesicle formation by functioning as a component of the COPI coat...

Research Grants10

  1. Dopamine Receptor Regulation by GRKs in Drug Abuse
    Richard Premont; Fiscal Year: 2007
    ..The results of these studies will define the role of GRK6 in setting the basal tone of dopamine signaling in the striatum. ..
  2. SIGNALING PATHWAYS OF ARF GTPASES AND ARF GAPS
    Richard Premont; Fiscal Year: 2005
    ..abstract_text> ..