Research Topics
Genomes and GenesSpecies | SALVATORE PIZZOSummaryAffiliation: Duke University Medical Center Country: USA Publications
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Publications
Annexin 2/factor xa-mediated signal transductionSalvatore V Pizzo
Duke University Medical Center, Chair, Department of Pathology, M301 Davison Bldg, Box 3712, Durham, NC 27710-0001, USA
Circ Res 102:389-91. 2008
Ligation of cancer cell surface GRP78 with antibodies directed against its COOH-terminal domain up-regulates p53 activity and promotes apoptosisUma Kant Misra
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
Mol Cancer Ther 8:1350-62. 2009..Antibodies directed against the COOH-terminal domain may play a therapeutic role in cancer patients whose tumors trigger the production of autoantibodies directed against the NH(2)-terminal domain of GRP78...- The effects of varying dietary carbohydrate and fat content on survival in a murine LNCaP prostate cancer xenograft modelJohn C Mavropoulos
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
Cancer Prev Res (Phila) 2:557-65. 2009.... - Anti-tumor necrosis factor-alpha therapy augments dipeptidyl peptidase IV activity and decreases autoantibodies to GRP78/BIP and phosphoglucose isomerase in patients with rheumatoid arthritisJohn C Mavropoulos
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
J Rheumatol 32:2116-24. 2005..CONCLUSION: The reduced activity of DPP IV along with increased titers of circulating antibodies to GRP78 and PGI may play a role in the pathogenesis of RA and can be successfully modified by administration of adalimumab... - Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthaseSulene L Chi
Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
Cancer Res 67:4716-24. 2007..Thus, MAb3D5AB1 shows angiostatin-like properties superior to angiostatin and may be exploited in cancer chemotherapy... 
Characterization of the interaction between alpha2-macroglobulin and fibroblast growth factor-2: the role of hydrophobic interactionsSmitha Mathew
Department of Pathology, Box 3712, Duke University Medical Center, Durham, NC 27710, USA
Biochem J 374:123-9. 2003..Our results demonstrate that the interactions of FGF-2 with alpha2M* and HSPGs are biochemically distinct, suggesting that different FGF-2 sequences are involved...- The role of Grp 78 in alpha 2-macroglobulin-induced signal transduction. Evidence from RNA interference that the low density lipoprotein receptor-related protein is associated with, but not necessary for, GRP 78-mediated signal transductionUma K Misra
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 277:42082-7. 2002.... - Low-carbohydrate diets and prostate cancer: how low is "low enough"?Elizabeth M Masko
Duke University Medical Center, Box 2626, Durham, NC 27710, USA
Cancer Prev Res (Phila) 3:1124-31. 2010..55, respectively). Insulin was significantly lower in the 20% carbohydrate arm (P = 0.03). LAPC-4 xenograft mice fed a low-carbohydrate diet (10-20% carbohydrate kcal) had similar survival as mice consuming NCKD (0% carbohydrate kcal)... - Plasminogen structural domains exhibit different functions when associated with cell surface GRP78 or the voltage-dependent anion channelMario Gonzalez-Gronow
Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
J Biol Chem 282:32811-20. 2007..We show that kringle 5 inhibits 1-LN cell proliferation and promotes caspase-7 activity by a mechanism that requires binding to cell surface voltage-dependent anion channel and is inhibited by human hexokinase I... - Carbohydrate restriction, prostate cancer growth, and the insulin-like growth factor axisStephen J Freedland
Department of Surgery, Durham VA Medical Center, Durham, North Carolina 27710, USA
Prostate 68:11-9. 2008..Recent evidence suggests carbohydrate intake may influence prostate cancer biology. We tested whether a no-carbohydrate ketogenic diet (NCKD) would delay prostate cancer growth relative to Western and low-fat diets in a xenograft model... - Prostate cancer cell proliferation in vitro is modulated by antibodies against glucose-regulated protein 78 isolated from patient serumMario Gonzalez-Gronow
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
Cancer Res 66:11424-31. 2006..Furthermore, increasing concentrations of human anti-GRP78 IgG show a dose-dependent protective effect on apoptosis induced by tumor necrosis factor alpha... - Dipeptidyl peptidase IV (DPP IV/CD26) is a cell-surface plasminogen receptorMario Gonzalez-Gronow
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
Front Biosci 13:1610-8. 2008.... - Association of plasminogen with dipeptidyl peptidase IV and Na+/H+ exchanger isoform NHE3 regulates invasion of human 1-LN prostate tumor cellsMario Gonzalez-Gronow
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 280:27173-8. 2005..These associations suggest that Pg has the potential to simultaneously regulate calcium signaling pathways and Na+/H+ exchanges necessary for tumor cell proliferation and invasiveness... - Soluble alpha2-macroglobulin receptor is increased in endotracheal aspirates from infants and children after cardiopulmonary bypassEric A Williams
Department of Pediatrics, Duke University Medical Center, Durham, NC 27710, USA
J Thorac Cardiovasc Surg 129:1098-103. 2005..Our aim was to determine whether the cytokine binding protein alpha 2 -macroglobulin and its soluble receptor were upregulated in endotracheal aspirates from infants and children undergoing cardiopulmonary bypass... - Angiostatin directly inhibits human prostate tumor cell invasion by blocking plasminogen binding to its cellular receptor, CD26Mario Gonzalez-Gronow
Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
Exp Cell Res 303:22-31. 2005..These studies suggest that, in addition to its ability to inhibit tumor vascularization, angiostatin 2epsilon may also directly block tumor metastasis... - The mechanism of action of angiostatin: can you teach an old dog new tricks?Tammy L Moser
Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
Thromb Haemost 87:394-401. 2002..This review will describe what is known about the mechanism of action of angiostatin from the current literature... - Antibodies against the voltage-dependent anion channel (VDAC) and its protective ligand hexokinase-I in children with autismMario Gonzalez-Gronow
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
J Neuroimmunol 227:153-61. 2010..Because VDAC and hexokinase-I are essential for brain protection from ischemic damage, the presence of these autoantibodies suggests a possible causal role in the neurologic pathogenesis of autism... - The voltage-dependent anion channel is a receptor for plasminogen kringle 5 on human endothelial cellsMario Gonzalez-Gronow
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 278:27312-8. 2003..K5 binding to endothelial cells also induces a decrease in intracellular pH and hyperpolarization of the mitochondrial membrane. These studies suggest that VDAC is a receptor for K5... - Tissue factor is the receptor for plasminogen type 1 on 1-LN human prostate cancer cellsMario Gonzalez-Gronow
Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
Blood 99:4562-7. 2002..The current studies suggest that Pg oligosaccharide chains regulate the binding of Pg 1 and Pg 2 to separate receptors on the cell surface... - Patterns of GRP78 and MTJ1 expression in primary cutaneous malignant melanomaJohn A Papalas
Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
Mod Pathol 23:134-43. 2010.... - GRP78: a multifunctional receptor on the cell surfaceMario Gonzalez-Gronow
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
Antioxid Redox Signal 11:2299-306. 2009..Here, we discuss the significance of these associations, and the possible mechanisms involved in the transportation of GRP78 from the cytosol to the cell surface... - GRP78: a chaperone with diverse roles beyond the endoplasmic reticulumQuintin J Quinones
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
Histol Histopathol 23:1409-16. 2008.... - Cell surface adenosine deaminase binds and stimulates plasminogen activation on 1-LN human prostate cancer cellsMario Gonzalez-Gronow
Department of Pathology, Box 3712, Duke University Medical Center, Durham, NC 27710, USA
J Biol Chem 279:20993-8. 2004..Thus, ADA may be a factor regulating events in prostate cancer cells that occur when Pg binds to the cell surface and is activated... - Increased immunogenicity of HIV envelope subunit complexed with alpha2-macroglobulin when combined with monophosphoryl lipid A and GM-CSFHua Xin Liao
Department of Medicine, Duke Human Vaccine Institute, Duke University Medical Center, Box 3258, Durham, NC 27710, USA
Vaccine 20:2396-403. 2002.... - Is there a role for a low-carbohydrate ketogenic diet in the management of prostate cancer?John C Mavropoulos
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
Urology 68:15-8. 2006 - Cell surface F1Fo ATP synthase: a new paradigm?Sulene L Chi
Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
Ann Med 38:429-38. 2006..This review addresses the expression, interactions, functions, and consequences of inhibition of cell surface ATP synthase, an enzyme now displaying a shift in paradigm, as well as of location... - SREC-I, a type F scavenger receptor, is an endocytic receptor for calreticulinBrent Berwin
Department of Pathology and Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 279:51250-7. 2004..These data identify a novel class of ligands for SREC-I and provide insight into the mechanisms by which APCs and potentially endothelial cells traffic chaperone/antigen complexes... - Alpha 2-macroglobulin binds CpG oligodeoxynucleotides and enhances their immunostimulatory properties by a receptor-dependent mechanismRyan B Anderson
Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
J Leukoc Biol 83:381-92. 2008..CpG ODN bound to alpha 2M* are also protected from degradation by nucleases. This novel targeting technology may improve CpG ODN-based therapeutics by increasing efficacy at reduced doses, thus reducing side-effects and cost... - Coordinate regulation of forskolin-induced cellular proliferation in macrophages by protein kinase A/cAMP-response element-binding protein (CREB) and Epac1-Rap1 signaling: effects of silencing CREB gene expression on Akt activationUma K Misra
Department of Pathology, Duke University, Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 280:38276-89. 2005..We conclude that forskolin mediates cellular proliferation via cAMP-dependent activation of both PKA and Epac... - Ligation of cell surface GRP78 with antibody directed against the COOH-terminal domain of GRP78 suppresses Ras/MAPK and PI 3-kinase/AKT signaling while promoting caspase activation in human prostate cancer cellsUma K Misra
Department of Pathology, Duke University Medical Center, Durham, NC, USA
Cancer Biol Ther 9:142-52. 2010..We conclude that antibody directed against the COOH-terminal domain of GRP78 may prove useful as a pan suppressor of proliferative/survival signaling in cancer cells expressing GRP78 on their cell surface... - Activation of Akt/PDK signaling in macrophages upon binding of receptor-recognized forms of alpha2-macroglobulin to its cellular receptor: effect of silencing the CREB geneU K Misra
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
J Cell Biochem 93:1020-32. 2004..We conclude that in murine peritoneal macrophages, alpha2M*-induced increase of cAMP is involved in cellular proliferation and this process is mediated by the PI 3-kinase signaling cascade... - Mast cell activators: a new class of highly effective vaccine adjuvantsJames B McLachlan
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
Nat Med 14:536-41. 2008..Moreover, they highlight MC activators as a new class of vaccine adjuvants, capable of inducing protective antigen-specific immune responses through needle-free routes of administration... - The cAMP-activated GTP exchange factor, Epac1 upregulates plasma membrane and nuclear Akt kinase activities in 8-CPT-2-O-Me-cAMP-stimulated macrophages: Gene silencing of the cAMP-activated GTP exchange Epac1 prevents 8-CPT-2-O-Me-cAMP activation of Akt aUma K Misra
Department of Pathology, M301 Davison Building, Duke University Medical Center, Durham, North Carolina 27710, United States
Cell Signal 20:1459-70. 2008..In conclusion, we show that cAMP-induced Akt kinase activation and cellular proliferation is mediated by Epac1 which appears to function as an accessory protein for Akt activation... - Angiostatin is directly cytotoxic to tumor cells at low extracellular pH: a mechanism dependent on cell surface-associated ATP synthaseSulene L Chi
Department of Pathology, Duke University Medical Center, Research Drive, Durham, NC 27710, USA
Cancer Res 66:875-82. 2006..Furthermore, these data provide evidence that extracellular ATP synthase plays a role in regulating pH(i) in cells challenged by acidosis... - Protease-activated receptor-2 signaling triggers dendritic cell developmentRyan C Fields
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA
Am J Pathol 162:1817-22. 2003..These results suggest that endogenous serine proteases stimulate DC development in vitro. Thus, serine proteases may help trigger adaptive immune responses in vivo... - Incorporation of low molecular weight molecules into alpha(2)-macroglobulin by nucleophilic exchangeJennifer E Bond
Department of Pathology, Duke University Medical Center, Box 3712, Durham, NC 27710, USA
Biochem Biophys Res Commun 357:433-8. 2007..2 mol GTP-TR/mol alpha(2)M( *). PAGE showed that 70-90% of the GTP-TR is bound in a SDS/2-mercaptoethanol resistant manner. Guanosine, adenosine, and imidazole competed with GTP-TR to form complexes with alpha(2)M( *)... - An Inhibitor of the F1 subunit of ATP synthase (IF1) modulates the activity of angiostatin on the endothelial cell surfaceNick R Burwick
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 280:1740-5. 2005..These data suggest that there is a relationship between the binding sites of IF1 and angiostatin on ATP synthase and that IF1 could be employed to modulate angiogenesis... - Angiostatin's molecular mechanism: aspects of specificity and regulation elucidatedMiriam L Wahl
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
J Cell Biochem 96:242-61. 2005.... - Cytokine profiling for prediction of symptomatic radiation-induced lung injuryJustin P Hart
Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
Int J Radiat Oncol Biol Phys 63:1448-54. 2005..Further studies are necessary to determine whether IL-8 levels are predictive of SRILI in a prospective trial and whether this marker might be used to determine patient eligibility for dose escalation... - The properties of rabbit alpha1-macroglobulin upon activation are distinct from those of rabbit and human alpha2-macroglobulinsAgnieszka Banbula
Department of Pathology, Box 3712, Duke University Medical Center, Durham, NC 27710, USA
J Biochem (Tokyo) 138:527-37. 2005..This suggests that rabbit alpha1M may not play the same proteinase-inhibiting physiological role as human alpha2M... - Angiostatin and anti-angiogenic therapy in human diseaseMiriam L Wahl
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
Recent Prog Horm Res 59:73-104. 2004.... - Antigen delivery by alpha(2)-macroglobulin enhances the cytotoxic T lymphocyte responseEdith V Bowers
Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
J Leukoc Biol 86:1259-68. 2009..These results support alpha(2)M* as an effective antigen-delivery system that may be particularly useful for vaccines based on weakly immunogenic subunits or requiring dose sparing... - Cutting edge: CD91-independent cross-presentation of GRP94(gp96)-associated peptidesBrent Berwin
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
J Immunol 168:4282-6. 2002..These data identify a CD91-independent, GRP94 internalization pathway that functions in peptide Ag re-presentation... - Probing the stability of native and activated forms of alpha2-macroglobulinSteven J Kaczowka
Department of Pathology, Duke University Medical Center, Box 3712, Durham, NC 27710, USA
Int J Biol Macromol 42:62-7. 2008..Differential scanning calorimetry and urea gel electrophoresis demonstrate that both bait region cleavage and covalent incorporation of protein ligands in the thioester pocket play critical roles in the stability of alpha2M complexes... - Interaction between TCL1 and Epac1 in the activation of Akt kinases in plasma membranes and nuclei of 8-CPT-2-O-Me-cAMP-stimulated macrophagesUma K Misra
Department of Pathology, Duke University Medical Center, Durham, NC 27710, United States
Cell Signal 20:130-8. 2008..Our studies suggest that a ternary complex of TCL1, Epac1, and Akt forms in activated macrophages both promoting Akt activation and regulating intracellular distribution of Akt... - A CD91-positive subset of CD11c+ blood dendritic cells: characterization of the APC that functions to enhance adaptive immune responses against CD91-targeted antigensJustin P Hart
Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
J Immunol 172:70-8. 2004..Therefore, CD11c(+) lin(-) DC use CD91/LRP to facilitate the uptake and subsequent presentation of an array of Ags complexed within the CD91/LRP ligand, the activated form of alpha2-macroglobulin (alpha2M*)... - Scavenger receptor-A mediates gp96/GRP94 and calreticulin internalization by antigen-presenting cellsBrent Berwin
Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
EMBO J 22:6127-36. 2003..In addition, macrophages derived from SR-A-/- mice were substantially impaired in gp96 binding and uptake. These data identify new roles for SR-A in the regulation of cellular responses to heat shock proteins... - Mast cell-derived tumor necrosis factor induces hypertrophy of draining lymph nodes during infectionJames B McLachlan
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA
Nat Immunol 4:1199-205. 2003..These results show a critical function for peripheral mast cell-derived tumor necrosis factor in regulating the hypertrophy of draining lymph nodes during infection... - New member of the trefoil factor family of proteins is an alpha-macroglobulin protease inhibitorIda B Thøgersen
Department of Molecular and Structural Biology, University of Arhus, Gustav Wieds Vej 10C, Aarhus, Denmark
Biochim Biophys Acta 1598:131-9. 2002..The proximity of the receptor-binding and trefoil domains implied that the trefoil domain is similarly concealed before bait region cleavage... - Chaperones and diseaseJohn C Mavropoulos
N Engl J Med 353:2821-2; author reply 2821-2. 2005 - Endothelial cell surface ATP synthase-triggered caspase-apoptotic pathway is essential for k1-5-induced antiangiogenesisNiina Veitonmaki
Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden
Cancer Res 64:3679-86. 2004..Thus, our findings provide a mechanistic insight with respect to the angiostatic action and signaling pathway of K1-5 and angiostatin... - Streptokinase promotes development of dipeptidyl peptidase IV (CD26) autoantibodies after fibrinolytic therapy in myocardial infarction patientsMiguel Cuchacovich
Rheumatology Section, University of Chile Clinical Hospital, Santiago, Chile
Clin Diagn Lab Immunol 9:1253-9. 2002.... - Murine low-density lipoprotein receptor-related protein 1 (LRP) is required for phagocytosis of targets bearing LRP ligands but is not required for C1q-triggered enhancement of phagocytosisAnna P Lillis
Center for Vascular and Inflammatory Diseases and Department of Surgery, University of Maryland School of Medicine, Baltimore, MD 21201, USA
J Immunol 181:364-73. 2008....
Research Grants
- Alpha2-Macroglobulin-PA Complexes: Novel Anthrax Vaccin*SALVATORE PIZZO; Fiscal Year: 2003..These studies will provide a new anthrax vaccine candidate with both improved immunogenicity and decreased reactogenicity. ..
- Modulation of Angiogenesis Via the Angiostatin ReceptorSALVATORE PIZZO; Fiscal Year: 2005..Because of the exposed intravascular localization of the ATP synthesizing enzymes, this project will yield a collection of anti-angiogenic compounds with strong potential for rapid translation into clinical studies. ..
- CELL RECEPTORS IN COAGULATION AND ATHEROGENESISSALVATORE PIZZO; Fiscal Year: 1992..Human complement proteins do not inhibit proteinases and human alpha2M does not possess a cell lytic capability...
- CELL RECEPTORS IN COAGULATION AND ATHEROGENESISSALVATORE PIZZO; Fiscal Year: 1991..These studies were undertaken since cis-DDP is an important antitumor drug which possesses two amine groups. It was felt that cis-DDP might react with alpha 2-M in a manner analogous to MeNH2...
- CELL RECEPTORS IN COAGULATION AND ATHEROGENESISSALVATORE PIZZO; Fiscal Year: 1980..The kinetics of binding of growth factor to SMC will also be studied utilizing purified radiolabeled SMGF to determine if a receptor exists, its specificity, saturability and reversibility of binding. ..
- CELL RECEPTORS IN COAGULATION AND ATHEROGENESISSALVATORE PIZZO; Fiscal Year: 2006..Future studies will determine whether this pathway also is essential for alpha2M-antigen processing and presentation. ..