Research Topics
Genomes and GenesSpecies | D S PisetskySummaryAffiliation: Duke University Medical Center Country: USA Publications
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Publications
Antinuclear antibodies in rheumatic disease: a proposal for a function-based classificationD S Pisetsky
Medical Research Service, Durham Veterans Administration Medical Center, Durham, NC 27705, USA
Scand J Immunol 76:223-8. 2012..This classification provides a framework to elucidate the serological features of rheumatic disease and the often uncertain relationship between ANA expression and disease manifestations...
Microparticles as mediators and biomarkers of rheumatic diseaseDavid S Pisetsky
Medical Research Service, Durham Veterans Administration Medical Center, Durham, NC 27705, USA
Rheumatology (Oxford) 51:1737-46. 2012..MPs thus represent novel subcellular structures that can impact on the pathogenesis of rheumatic disease and serve as biomarkers of underlying cellular disturbances...- The origin and properties of extracellular DNA: from PAMP to DAMPDavid S Pisetsky
Medical Research Service, Durham Veterans Administration Medical Center, Durham, NC 27705, USA
Clin Immunol 144:32-40. 2012..Together, these findings suggest that the immune properties of DNA are mutable and diverse, reflecting its context and the array of attached molecules... - Cell death in the pathogenesis of immune-mediated diseases: the role of HMGB1 and DAMP-PAMP complexesD Pisetsky
Durham VA Medical Center, 508 Fulton Street, Durham, NC 27705, USA
Swiss Med Wkly 141:w13256. 2011.... - Effects of progesterone and estradiol sex hormones on the release of microparticles by RAW 264.7 macrophages stimulated by Poly(I:C)David S Pisetsky
Department of Medicine, Duke University Medical Center, 151G Durham VA Medical Center, 508 Fulton Street, Durham, NC 27705, USA
Clin Vaccine Immunol 18:1420-6. 2011..Together, these results indicate that progesterone but not estradiol can inhibit particle release by stimulated macrophages and suggest a mechanism that may contribute to the immunomodulatory effects of this sex hormone... 
A walk on the beachDavid S Pisetsky
Duke University Medical Center; Durham, NC 27710, USA
Ann Intern Med 137:366-7. 2002- The blood nucleome in the pathogenesis of SLEDavid S Pisetsky
Medical Research Service, Durham Veterans Administration Medical Center, Durham, North Carolina 27705, USA
Autoimmun Rev 10:35-7. 2010..Together, these findings suggest that cell death is an important event in lupus pathogenesis and can provide a supply of blood DNA essential for immune complex formation... - Microparticles as autoadjuvants in the pathogenesis of SLEDavid S Pisetsky
Department of Medicine and Immunology, Duke University Medical Center, Medical Research Service, Durham VA Hospital, 151G Durham VAMC, 508 Fulton Street, Durham, NC 27705, USA
Nat Rev Rheumatol 6:368-72. 2010..We would therefore advance the idea that a model for SLE based on MP autoadjuvants can provide a new paradigm to elucidate the mechanisms by which DNA and RNA affect the immune system and critically influence B-cell fate... - The origin of extracellular DNA during the clearance of dead and dying cellsDavid S Pisetsky
Durham VA Medical Center, Duke University, Durham, NC 27705, USA
Autoimmunity 40:281-4. 2007..Together, these results indicate that, while apoptosis and necrosis can lead to a blood DNA response, this process requires macrophages and may be hormonally mediated... - A landmark study on treatment strategies for rheumatoid arthritisDavid S Pisetsky
Duke University Medical Center, Durham, North Carolina, USA
Arthritis Rheum 58:S123-5. 2008 - Clinician's comment on the management of pain in arthritisDavid S Pisetsky
Durham VA Medical Center, 508 Fulton Street, Durham, NC 27705, USA
Health Psychol 26:657-9. 2007..These factors include the following: diagnosis, disease activity, damage, disease stage, patient age and demographics, presence of comorbidities, and availability of alternative or adjunctive approaches... - Role of Toll-like receptors in HMGB1 release from macrophagesDavid S Pisetsky
Medical Research Service, Durham VA Medical Center, Durham, North Carolina 27705, USA
Ann N Y Acad Sci 1109:58-65. 2007..Because the kinetics of HMGB1 release differs from that of a conventional cytokine, it provides a broader therapeutic window and may be an important new target of therapy for inflammatory, autoimmune, and infectious diseases... - The role of nuclear macromolecules in innate immunityDavid S Pisetsky
Durham VA Medical Center and Duke University Medical Center, Box 151G, 508 Fulton St, Durham, NC 27705, USA
Proc Am Thorac Soc 4:258-62. 2007..For both DNA and HMGB1, the immune properties may therefore reflect the array of other endogenous as well as exogenous molecules present... - The generation of extracellular DNA in SLE: the role of death and sexD S Pisetsky
Medical Research Service, Division of Rheumatology and Immunology, Duke University Medical Center, Durham VA Hospital, 508 Fulton Street, Durham, NC 27705, USA
Scand J Immunol 64:200-4. 2006..Together, these studies clarify the origin of extracellular DNA circulating in the blood in SLE and suggest steps in this process that can be interdicted by novel therapy... - The immune response to cell death in SLEDavid S Pisetsky
Medical Research Service, 151G Durham VA Hospital, Division of Rheumatology and Immunology, Duke University Medical Center, 508 Fulton Street, Durham, NC 27705, USA
Autoimmun Rev 3:500-4. 2004.... - Immune responses to DNA in normal and aberrant immunityD S Pisetsky
Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
Immunol Res 22:119-26. 2000.... - Anti-DNA and autoantibodiesD S Pisetsky
Division Rheumatology, Allergy and Clinical Immunology, Durham VA and Duke University Medical Centers, North Carolina 27705, USA
Curr Opin Rheumatol 12:364-8. 2000..The clinical measurement of ANAs, although valuable in assessing diagnosis and prognosis, must nevertheless be interpreted with caution because ANAs, despite their disease associations, can occur in healthy individuals... - Immune response to DNA in systemic lupus erythematosusD S Pisetsky
Departments of Medicine and Immunology, Durham, VA, USA
Isr Med Assoc J 3:850-3. 2001..To the extent that self-DNA drives responses in SLE, anti-DNA production in this disease may reflect a failure of this suppression. The recognition of DNA's immune activities thus suggests novel possibilities for disease pathogenesis... - The influence of DNA size on the binding of antibodies to DNA in the sera of normal human subjects and patients with systemic lupus erythematosus (SLE)D S Pisetsky
Medical Research Service, Department of Veterans Affairs Medical Center, Division of Rheumatology, Allergy and Clinical Immunology, Duke University Medical Center, Durham, NC, USA
Clin Exp Immunol 116:354-9. 1999..These results suggest that SLE and NHS anti-DNA react with different antigenic determinants on DNA, as shown by cross-reactivity as well as size dependency in solid-phase assays... - The influence of base sequence on the immunostimulatory properties of DNAD S Pisetsky
Medical Service Durham VA Medical Center and Division of Rheumatology, Allergy and Clinical Immunology, Duke University Medical Center, NC 27705, USA
Immunol Res 19:35-46. 1999..The array of these sequences may determine the overall immune activity of a DNA molecule and affect such processes as host defense against infection as well as the use of plasmids and synthetic oligonucleotides to treat disease... - The influence of lipofectin on the in vitro stimulation of murine spleen cells by bacterial DNA and plasmid DNA vectorsD S Pisetsky
Medical Service, Durham VA Medical Center, NC 27705, USA
J Interferon Cytokine Res 19:1219-26. 1999..These findings indicate that lipofectin can increase the in vitro immunostimulatory effects of bacterial and plasmid DNA, although the magnitude of the increase may vary among responses... - Influence of backbone chemistry on immune activation by synthetic oligonucleotidesD S Pisetsky
Medical Research Service, Durham Veterans Administration Hospital, Durham, NC 27705, USA
Biochem Pharmacol 58:1981-8. 1999..While suggesting differences in the structure-function relationships of nucleic acids in their immune activities, these findings also raise the possibility of the design of agents with specific patterns of immune modulation... - The role of bacterial DNA in autoantibody inductionD S Pisetsky
VA Medical Center, Durham, NC 27705, USA
Curr Top Microbiol Immunol 247:143-55. 2000..Future studies will hopefully both determine the role of foreign nucleic acids in the induction of autoantibodies and lead to strategies for their elimination... - Interleukin-1, tumor necrosis factor alpha, and interleukin-17 synergistically up-regulate nitric oxide and prostaglandin E2 production in explants of human osteoarthritic knee menisciA Legrand
Duke University Medical Center, Durham, North Carolina 27710, USA
Arthritis Rheum 44:2078-83. 2001..NO and PGE2 exhibited reciprocal regulatory effects on one another, suggesting that pharmaceutical agents designed to inhibit NOS2 or COX-2 production may in fact be influencing both pathways... - The effects of static and intermittent compression on nitric oxide production in articular cartilage explantsB Fermor
Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
J Orthop Res 19:729-37. 2001..These findings indicate that NO production by chondrocytes is influenced by mechanical compression in vitro and suggest that biomechanical factors may in part regulate NO production in vivo... - The effect of dynamic mechanical compression on nitric oxide production in the meniscusC Fink
Department of Surgery, Division of Orthopaedic Surgery, Duke University Medical Center, Durham, NC 27710, USA
Osteoarthritis Cartilage 9:481-7. 2001.... - Host response to infection: the role of CpG DNA in induction of cyclooxygenase 2 and nitric oxide synthase 2 in murine macrophagesD K Ghosh
Department of Medicine, Veterans Affairs and Duke University Medical Centers, Durham, North Carolina 27705, USA
Infect Immun 69:7703-10. 2001..Thus, analogues of microbial DNA (i.e., CpG DNA) activate mouse macrophage lineage cells for the expression of NOS2 and COX2, with the production of NO and that of PGE(2) occurring in an interdependent manner... - Mechanical stress and nitric oxide influence leukotriene production in cartilageB Fermor
Department of Surgery, Division of Orthopedic Surgery, Duke University Medical Center, Durham, NC 27710, USA
Biochem Biophys Res Commun 285:806-10. 2001..These findings provide a direct link between mechanical stress and inflammation in cartilage and may have implications in the pathogenesis and treatment of arthritis... - The role of the macrophage scavenger receptor in immune stimulation by bacterial DNA and synthetic oligonucleotidesF G Zhu
Medical Research Service, Durham Veterans Administration Hospital and Division of Rheumatology, Allergy and Clinical Immunology, Duke University Medical Center, Durham, NC, USA
Immunology 103:226-34. 2001..Together, these findings indicate that, while the SRA may bind DNA, this receptor is not essential for the uptake of CpG DNA or its immunostimulatory activity... - Role of the heat shock protein 90 in immune response stimulation by bacterial DNA and synthetic oligonucleotidesF G Zhu
Medical Research Service, Durham Veterans Administration Hospital, and Division of Rheumatology, Allergy and Clinical Immunology, Duke University Medical Center, Durham, North Carolina 27705, USA
Infect Immun 69:5546-52. 2001.... - Differences in V kappa gene utilization and VH CDR3 sequence among anti-DNA from C3H-lpr mice and lupus mice with nephritisM K Wloch
Department of Medicine, Duke University Medical Center, Durham, USA
Eur J Immunol 26:2225-33. 1996..These results indicate that anti-DNA from C3H-lpr mice differ from anti-DNA from lupus mice with nephritis in patterns of V gene expression and suggest a molecular basis for the lack of pathogenicity of anti-DNA in these mice... - Enhanced renal leukotriene production in murine lupus: role of lipoxygenase metabolitesR F Spurney
Department of Medicine, Duke University, Durham, North Carolina
Kidney Int 39:95-102. 1991..Increased leukotriene production within the kidney may therefore be important in the pathogenesis of lupus nephritis... - Molecular properties of anti-DNA induced in preautoimmune NZB/W mice by immunization with bacterial DNAM K Wloch
Department of Medicine, Duke University Medical Center, Durham, NC 27705, USA
J Immunol 158:4500-6. 1997..The findings suggest that preautoimmune NZB/W mice have immunoregulatory defects that allow activation of mammalian dsDNA reactive B cells by bacterial DNA... - Inhibition of murine macrophage IL-12 production by natural and synthetic DNAD S Pisetsky
Durham VA Medical Center, Durham, North Carolina, 27705, USA
Clin Immunol 96:198-204. 2000..Together, these results indicate that DNA has inhibitory properties, suggesting that mammalian DNA could limit immune activation during inflammation and counteract the effects of bacterial DNA... - Release of DNA from dead and dying lymphocyte and monocyte cell lines in vitroJ-J Choi
Medical Research Service, Durham VA Medical Center, Division of Rheumatology, Duke University Medical Center, 508 Fulton Street, Durham, NC 27705, USA
Scand J Immunol 60:159-66. 2004..These studies suggest that extracellular release of DNA is a consequence of apoptosis and may account for some of the DNA in the blood... 
The role of nitric oxide in the pathogenesis of spontaneous murine autoimmune disease: increased nitric oxide production and nitric oxide synthase expression in MRL-lpr/lpr mice, and reduction of spontaneous glomerulonephritis and arthritis by orally admiJ B Weinberg
Department of Medicine, Veterans Administration Medical Center, Durham, North Carolina 27705
J Exp Med 179:651-60. 1994..These results suggest that elevated nitric oxide production could be important in the pathogenesis of autoimmunity, and that treatments to block the production of nitric oxide or block its effects might be valuable therapeutically...- The use of fluorometric assays to assess the immune response to DNA in murine systemic lupus erythematosusL Björkman
Medical Research Service, Durham Veterans Administration Hospital and Division of Rheumatology, Duke University Medical Center, Durham, NC, USA
Scand J Immunol 57:525-33. 2003..Together, our results suggest that a fluorometric dye can accurately monitor DNA and anti-DNA antibody levels in SLE and may provide important information on immunopathogenesis... - The role of extracellular DNA in autoimmunity in SLEK Y Su
Durham VA Medical Center and Duke University Medical Center, Durham, NC 27705, USA
Scand J Immunol 70:175-83. 2009..Together, these findings indicate that the generation of extracellular DNA in SLE can result from cell death and that steps in this process represent potential targets for new therapies... - Deficiency of 5-lipoxygenase abolishes sex-related survival differences in MRL-lpr/lpr miceJ L Goulet
Division of Nephrology, Department of Medicine, Duke University Medical Center, Durham, NC 27705, USA
J Immunol 163:359-66. 1999..These findings suggest that the presence of a functional 5lo gene confers a survival advantage on male MRL-lpr/lpr mice and that, when 5LO function is inhibited, either genetically or pharmacologically, this advantage is abolished... - The role of cpg sequences in the induction of anti-DNA antibodiesD S Pisetsky
Durham VA and Duke University Medical Centers, Durham, North Carolina 27705, USA
Clin Immunol 100:157-63. 2001..These results thus extend the adjuvant effects of CpG sequences to self antigens and suggest mechanisms by which self and foreign antigens can interact in the generation of autoimmunity... - Stimulation of thymocyte proliferation by phosphorothioate DNA oligonucleotidesR B Mannon
Division of Nephrology, Duke University Medical Center, Durham, North Carolina, 27710, USA
Cell Immunol 201:14-21. 2000..These observations thus extend the range of actions of CpG DNA and suggest additional mechanisms for its function as an immunomodulatory agent or adjuvant... - Influence of hypoxia and reoxygenation on cytokine-induced production of proinflammatory mediators in articular cartilageJulie Cernanec
Duke University Medical Center, Durham, North Carolina 27710, USA
Arthritis Rheum 46:968-75. 2002..Hypoxia and reoxygenation can affect cytokine-induced proinflammatory mediator production, suggesting that oxygen tension may influence inflammation associated with cartilage injury and disease... - Antibodies to DNA: infection or genetics?D S Pisetsky
Division of Rheumatology and Immunology, Duke University Medical Center, Durham, North Carolina 27705, USA
Lupus 18:1176-80. 2009..Given the universal expression of DNA, this model suggests that many different kinds of infections could trigger pathogenic autoantibody responses in SLE, as well as induce flare... - Specificity and immunochemical properties of anti-DNA antibodies induced in normal mice by immunization with mammalian DNA with a CpG oligonucleotide as adjuvantTrinh T Tran
Division of Rheumatology, Duke University Medical Center, Durham, NC 27709, USA
Clin Immunol 109:278-87. 2003..Together, these results indicate that normal mice can produce autoantibodies to dsDNA, with a CpG ODN allowing the generation of antibodies resembling those in spontaneous autoimmunity... - The role of macrophages in the in vitro generation of extracellular DNA from apoptotic and necrotic cellsJin-Jung Choi
Medical Research Service, Durham VA Medical Center, Division of Rheumatology, Duke University Medical Center, Durham, NC 27705, USA
Immunology 115:55-62. 2005..Together, these results indicate that macrophages play an important role in the generation of extracellular DNA from dead and dying cells, with the effect dependent on how the cell died... - Induction of cyclooxygenase-2 by mechanical stress through a nitric oxide-regulated pathwayB Fermor
Department of Surgery, Division of Orthopaedic Surgery, Duke University Medical Center, NC 27710, USA
Osteoarthritis Cartilage 10:792-8. 2002.... - The content of DNA and RNA in microparticles released by Jurkat and HL-60 cells undergoing in vitro apoptosisCharles F Reich
Medical Research Service, 151G Durham VAMC, 508 Fulton Street, Durham, NC 27705, USA
Exp Cell Res 315:760-8. 2009.... - Serum, urinary, and salivary nitric oxide in rheumatoid arthritis: complexities of interpreting nitric oxide measuresJ Brice Weinberg
Veterans Affairs Medical Center, 508 Fulton Street, Durham, NC 27705, USA
Arthritis Res Ther 8:R140. 2006..Despite interest in the use of NO as a marker of disease activity, alterations in renal NOx clearance and fractional excretion in RA make it difficult to assess in vivo NO production even with strict dietary restriction of NOx intake... - Developments in the scientific understanding of lupusStacy P Ardoin
Department of Pediatrics, Duke University Medical Center, 2301 Erwin Road, Durham, NC 27710, USA
Arthritis Res Ther 10:218. 2008..Together, these findings point to new genetic and immunologic markers of disease as well as targets for new therapies... - The role of innate immunity in the induction of autoimmunityDavid S Pisetsky
Division of Rheumatology and Immunology, Duke University Medical Center, Durham, NC, USA
Autoimmun Rev 8:69-72. 2008..Together, these observations suggest that DNA can induce innate as well as adaptive immune responses and promote the pathogenesis of SLE because of its intrinsic immunostimulatory activity... - High-mobility group box protein 1 (HMGB1): an alarmin mediating the pathogenesis of rheumatic diseaseDavid S Pisetsky
Division of Rheumatology and Immunology, Duke University Medical Center, Durham, NC, USA
Arthritis Res Ther 10:209. 2008..New approaches to therapy for these diseases may involve strategies to inhibit HMGB1 release from cells, its interaction with receptors, and downstream signaling... - The role of cell death in the pathogenesis of autoimmune disease: HMGB1 and microparticles as intercellular mediators of inflammationStacy P Ardoin
Departments of Internal Medicine and Pediatrics, Divisions of Rheumatology and Pediatric Rheumatology, Duke University Medical Center, DUMC Box 3212, Durham, NC 27710, USA
Mod Rheumatol 18:319-26. 2008..Given their range of activity and association with active disease, both structures may prove to be targets for effective therapy in these and other disorders... - The expression of plasma nucleosomes in mice undergoing in vivo apoptosisNing Jiang
Division of Rheumatology, Duke University Medical Center, Durham, NC 27709, USA
Clin Immunol 106:139-47. 2003..These results suggest that plasma nucleosome levels reflect specific patterns of cell death and are not an invariable consequence of in vivo apoptosis or immune cell activation... - Mechanisms of Disease: the role of high-mobility group protein 1 in the pathogenesis of inflammatory arthritisWeiwen Jiang
Department of Medicine, Duke University Medical Center, Durham, NC 27705, USA
Nat Clin Pract Rheumatol 3:52-8. 2007..These studies identify a novel pathway in the pathogenesis of inflammatory arthritis, as well as a new target for biologic therapy... - Autoimmunity: the nuclear arsenal of autoimmunityDavid S Pisetsky
1151G Durham VA Hospital, Durham, NC 27705, USA
Immunol Cell Biol 85:344-5. 2007 - Microparticles as biomarkers in autoimmunity: from dust bin to center stageDavid S Pisetsky
Medical Research Service, Durham VA Medical Center, Division of Immunology and Rheumatology, Duke University Medical Center, 151G Durham VAMC, 508 Fulton Street, Durham, NC 27705, USA
Arthritis Res Ther 11:135. 2009..These findings suggest microparticles as novel biomarkers for autoimmunity, with levels reflecting events leading to their loss as well as production... - Use of SYTO 13, a fluorescent dye binding nucleic acids, for the detection of microparticles in in vitro systemsAnirudh J Ullal
Division of Rheumatology and Immunology, Department of Medicine, Duke University, Durham, North Carolina, USA
Cytometry A 77:294-301. 2010..Together, these findings indicate that the nucleic acid content of MPs provides the basis for their detection in in vitro systems and suggests the utility of fluorescent dyes like SYTO 13 for more sensitive quantitative assays... - The influence of oxygen tension on the induction of nitric oxide and prostaglandin E2 by mechanical stress in articular cartilageBeverley Fermor
Department of Surgery, Division of Orthopaedic Surgery, Box 3093, Duke University Medical Center, NC 27710, USA
Osteoarthritis Cartilage 13:935-41. 2005..The objective of this study was to determine the influence of oxygen tension on the induction of NO and PGE(2) production in articular cartilage in response to mechanical stress... - Microparticles as a source of extracellular DNADavid S Pisetsky
Medical Research Service, Durham VAMC, 151G, 508 Fulton Street, Durham, NC 27705, USA
Immunol Res 49:227-34. 2011..This DNA is antigenically active and can bind to lupus anti-DNA autoantibodies. These findings suggest that microparticles are an important source of extracellular DNA to serve as an autoantigen and autoadjuvant in SLE... - DNA as a marker of cell death in systemic lupus erythematosusDavid S Pisetsky
Division of Rheumatology and Immunology, Duke University Medical Center, Medical Research Service, Durham, NC, USA
Rheum Dis Clin North Am 30:575-87, x. 2004..These results indicate that circulating DNA may be a marker of cell death, although its levels likely reflect a complex process involving the interactions of macrophages with dead and dying cells... - OutOfDateDavid S Pisetsky
Division of Rheumatology, Allergy and Clinical Immunology at Duke University Medical Center, Durham, North Carolina 27705, USA
Pharos Alpha Omega Alpha Honor Med Soc 67:17-8. 2004 - Toll-like receptors in the pathogenesis of human diseaseDonald N Cook
Department of Medicine, Duke University Medical Center, and the Durham Veterans Administration Medical Center, Durham, North Carolina 27710, USA
Nat Immunol 5:975-9. 2004..As this body of data grows, it will provide new insights into disease pathogenesis as well as valuable information on the merits of various therapeutic options... - The effect of dexamethasone on the generation of plasma DNA from dead and dying cellsNing Jiang
Division of Rheumatology, Duke University Medical Center, Durham, North Carolina, USA
Am J Pathol 164:1751-9. 2004..These activities may be relevant to the efficacy of glucocorticoids in the treatment of inflammatory disease... - The release of microparticles by Jurkat leukemia T cells treated with staurosporine and related kinase inhibitors to induce apoptosisAnirudh J Ullal
Department of Medicine, Duke University, Durham, NC, USA
Apoptosis 15:586-96. 2010..Together, these results indicate that STS and UCN-01 induce MPs that are phenotypically distinct and reflect specific patterns of kinase inhibition during apoptosis... - Systemic lupus erythematosus and related diseasesTrinh T Tran
Division of Rheumatology, Duke University Medical Center, Durham, NC 27709, USA
Autoimmunity 37:301-4. 2004 - Early rheumatoid arthritisKate L Mitchell
Division of Rheumatology and Immunology, Duke University Medical Center, Durham, NC 27705, USA
Curr Opin Rheumatol 19:278-83. 2007..The purpose of current research is therefore to identify prognostic markers of early disease and to determine the role of aggressive treatment strategies in inducing remission in such patients... - B lymphocytes and systemic lupus erythematosusLisa G Criscione
Division of Rheumatology, Department of Medicine, Duke University Medical Center, 151G Durham VA Medical Center, 508 Fulton Street, Durham, NC 27705, USA
Curr Rheumatol Rep 5:264-9. 2003..New approaches to therapy aim to abrogate autoantibody production by targeting specific steps in B cell activation, including blockade of T cell costimulation... - Role of macrophages in the generation of circulating blood nucleosomes from dead and dying cellsNing Jiang
Division of Rheumatology, Duke University Medical Center, Durham, NC 27705, USA
Blood 102:2243-50. 2003..Together, these findings suggest that the generation of blood nucleosomes depends on macrophages, with apoptosis a concomitant of a high burden of dead and dying cells... - Effect of cytofectins on the immune response of murine macrophages to mammalian DNAFu-Gang Zhu
Durham Veterans Administration Hospital, Departments of Medicine and Immunology, Duke University Medical Center, Durham, NC 27705, USA
Immunology 109:255-62. 2003..Our findings indicate that macrophages vary in their response to DNA depending on uptake pathway, suggesting that activation by DNA reflects not only sequence but also context or intracellular location... - Mechanisms of activation of the RAW264.7 macrophage cell line by transfected mammalian DNAWeiwen Jiang
Department of Medicine, Division of Rheumatology and Immunology, Duke University, Durham, NC, USA
Cell Immunol 229:31-40. 2004..These data indicate that the immune activity of DNA is influenced by context or intracellular location and that, when transfected into cells, mammalian DNA can activate cells through signaling pathways similar to those of bacterial DNA... - The release of microparticles by RAW 264.7 macrophage cells stimulated with TLR ligandsJulie Gauley
Duke University Medical Center, Division of Rheumatology and Immunology, Durham, North Carolina, USA
J Leukoc Biol 87:1115-23. 2010..Together, these experiments demonstrate that TLR stimulation of macrophages can lead to MP release, and NO plays a key role in this response... - The effects of heparins on the liver: application of mechanistic serum biomarkers in a randomized study in healthy volunteersA H Harrill
Hamner University of North Carolina Institute for Drug Safety Sciences, The Hamner Institutes for Health Sciences, Research Triangle Park, NC, USA
Clin Pharmacol Ther 92:214-20. 2012..Biomarker profiles did not differ significantly across heparin treatments. We conclude that heparins as a class cause self-limited and mild hepatocyte necrosis with secondary activation of an innate immune response... - CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunityS Sato
Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA
J Immunol 157:4371-8. 1996.... - Genetic control of inflammatory arthritis and glomerulonephritis in congenic lpr mice and their F1 hybridsG S Gilkeson
Medical Research Service, Durham VA Medical Center, North Carolina
J Autoimmun 4:595-606. 1991..These results indicate that renal disease and arthritis develop independently in lpr mice, possibly on a genetic basis, and that the presence and titer of autoantibodies do not correlate with tissue injury... - Genetic analysis of MRL-lpr mice: relationship of the Fas apoptosis gene to disease manifestations and renal disease-modifying lociM L Watson
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710
J Exp Med 176:1645-56. 1992..Moreover, this study provides a model for dissecting the complex genetic interactions that result in manifestations of autoimmune disease... - Regulation of matrix turnover in meniscal explants: role of mechanical stress, interleukin-1, and nitric oxideSang-Jin Shin
Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
J Appl Physiol 95:308-13. 2003..These finding suggest that IL-1 may modulate the effects of mechanical stress on extracellular matrix turnover through a pathway that is dependent on NO... - The effect of inflammation on the generation of plasma DNA from dead and dying cells in the peritoneumNing Jiang
Division of Rheumatology, Duke University Medical Center, Box 151G, 508 Fulton St, Durham, NC 27705, USA
J Leukoc Biol 77:296-302. 2005.... - The role of IFN-alpha and nitric oxide in the release of HMGB1 by RAW 264.7 cells stimulated with polyinosinic-polycytidylic acid or lipopolysaccharideWeiwen Jiang
Division of Rheumatology and Immunology, Department of Medicine, Duke University, Durham, NC 27710, USA
J Immunol 177:3337-43. 2006..7 cells. Together, these experiments indicate that, although both LPS and poly(I:C) induce HMGB1 release from RAW 264.7 cells and murine macrophages, the response is differentially dependent on NO and IFN-alpha... - The binding of sera of patients with SLE to bacterial and mammalian DNAKimberly J Hamilton
Department of Medicine, Medical Research Service, Durham VA Hospital, 151G, 508 Fulton St, Durham, NC 27705, USA
Clin Immunol 118:209-18. 2006..These findings suggest that anti-DNA antibodies vary in specificity and are consistent with a role of both foreign and self-DNA in anti-DNA induction... - The effects of CpG DNA on HMGB1 release by murine macrophage cell linesWeiwen Jiang
Division of Rheumatology and Immunology, Department of Medicine, Duke University, Durham, NC 27705, USA
J Leukoc Biol 78:930-6. 2005.... - The extracellular release of HMGB1 during apoptotic cell deathCharles W Bell
Division of Rheumatology and Immunology, Duke University Medical Center, and Medical Research Service, Durham Veterans Affairs Hospital, Durham, North Carolina 27705, USA
Am J Physiol Cell Physiol 291:C1318-25. 2006..Together, these studies indicate that HMGB1 release can occur during the course of apoptosis as well as necrosis and suggest that the release process may vary with cell type... - The induction of HMGB1 release from RAW 264.7 cells by transfected DNAWeiwen Jiang
Division of Rheumatology and Immunology, Department of Medicine, Duke University, Durham, NC 27710, USA
Mol Immunol 45:2038-44. 2008.... - Inhibition of murine macrophage nitric oxide production by synthetic oligonucleotidesFu-Gang Zhu
Medical Research Service, Durham Veterans Administration Hospital and Division of Rheumatology, Allergy and Clinical Immunology, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27705, USA
J Leukoc Biol 71:686-94. 2002..Together, these observations indicate that Ps ODN can inhibit macrophage production of inflammatory mediators, suggesting a role of these compounds as immunomodulatory agents... - The release of DNA into the plasma of mice following hepatic cell death by apoptosis and necrosisTrinh T Tran
Department of Medicine, Division of Rheumatology and Immunology, Duke University Medical Center, Durham, NC, USA
Biomarkers 13:184-200. 2008..These results indicate that increased blood DNA is common in hepatotoxic injury and is a feature of both apoptotic and necrotic death... - The effects of intravenous doxycycline therapy for rheumatoid arthritis: a randomized, double-blind, placebo-controlled trialE W St Clair
Duke University Medical Center, Durham, North Carolina 27710, USA
Arthritis Rheum 44:1043-7. 2001..CONCLUSION: Although IV doxycycline therapy was generally well-tolerated by patients in this trial, it did not show any evidence of reducing disease activity or collagen crosslink production... - The role of microparticles in inflammation and thrombosisS P Ardoin
Division of Rheumatology and Immunology, Duke University Medical Center, Medical Research Service, Durham VA Hospital, Durham, NC, USA
Scand J Immunol 66:159-65. 2007..As biomarkers, MP allow access to usually inaccessible tissues such as the endothelium. Further research will hopefully lead to interventions targeting MP release and function... - Induction of immune activation by a novel immunomodulatory oligonucleotide without thymocyte apoptosisWeiwen Jiang
Division of Rheumatology and Department of Immunology, Duke University Medical Center, Durham, NC, USA
Biochem Biophys Res Commun 318:60-6. 2004..The levels of corticosterone induced by HYB2048 were also significantly lower than those induced by LPS. This pattern of activation could distinguish CpG DNA from LPS in its effects on the immune system... - Inhibition of murine dendritic cell activation by synthetic phosphorothioate oligodeoxynucleotidesFu-Gang Zhu
Medical Research Service, Durham Veterans Administration Hospital and Division of Rheumatology, Allergy and Clinical Immunology, Duke University Medical Center, Durham, North Carolina, USA
J Leukoc Biol 72:1154-63. 2002..Together, these observations extend the range of action of inhibitory ODN to DC and suggest a role of these compounds as immunomodulatory agents... - The effects of cyclic mechanical strain and tumor necrosis factor alpha on the response of cells of the meniscusBeverley Fermor
Department of Surgery, Division of Orthopaedic Surgery, Duke University Medical Center, Durham, NC 27710, USA
Osteoarthritis Cartilage 12:956-62. 2004.... - Informed consent in a clinical trial of a novel treatment for rheumatoid arthritisLisa G Criscione
Duke University Medical Center, Durham, North Carolina 27710, USA
Arthritis Rheum 49:361-7. 2003..Further education of potential participants in clinical trials may be required to achieve valid informed consent... - Glomerular type 1 angiotensin receptors augment kidney injury and inflammation in murine autoimmune nephritisSteven D Crowley
Department of Medicine, Division of Nephrology, Duke University Medical Center, and Durham VA Medical Center, Durham, North Carolina 27705, USA
J Clin Invest 119:943-53. 2009..Since AT1A-deficient lpr mice had low blood pressure, these findings suggest that activation of type 1 angiotensin receptors in the glomerulus is sufficient to accelerate renal injury and inflammation in the absence of hypertension... - The relationship between apoptosis and high-mobility group protein 1 release from murine macrophages stimulated with lipopolysaccharide or polyinosinic-polycytidylic acidWeiwen Jiang
Division of Rheumatology and Immunology, Department of Medicine, Duke University, and Medical Research Services, Durham Veterans Affairs Medical Center, NC 27710, USA
J Immunol 178:6495-503. 2007..Together, these results indicate that HMGB1 release from macrophages is correlated with the occurrence of apoptosis and suggest that these processes reflect common mechanisms and can occur concomitantly... - Enhancing immunogenicity by CpG DNAWeiwen Jiang
Durham VA Medical Center, 508 Fulton Street, PO Box 151G, Durham, NC 27705, USA
Curr Opin Mol Ther 5:180-5. 2003..Furthermore, CpG DNA can provide protection against pathogens in animal models and has therapeutic applications in clinical settings such as in cancer and allergy... - Induction of plasma (TRAIL), TNFR-2, Fas ligand, and plasma microparticles after human immunodeficiency virus type 1 (HIV-1) transmission: implications for HIV-1 vaccine designNancy Gasper-Smith
Duke Human Vaccine Institute, Department of Medicine, Duke University School of Medicine, Durham, North Carolina 27710, USA
J Virol 82:7700-10. 2008.... - In vitro assay of immunostimulatory activities of plasmid vectorsWeiwen Jiang
Duke University, Durham, NC, USA
Methods Mol Med 127:55-70. 2006.... - Rheumatology in 2006: crossroads or crisis?David S Pisetsky
Departmentof Medicine, Division of Rheumatology and Immunology, Duke UniversityMedical Center, Durham, NC, USA
Bull NYU Hosp Jt Dis 64:9-11. 2006..Fortunately, the opportunities for the future remain very bright because of burgeoning research in biomedicine and outcomes assessment as well as progress in developing personalized medicine to individualize treatment better... - Pivotal advance: inhibition of HMGB1 nuclear translocation as a mechanism for the anti-rheumatic effects of gold sodium thiomalateCecilia K Zetterström
Department of Woman and Child Health, Pediatric Rheumatology Research Unit, Karolinska Institutet Karolinska University Hospital, Stockholm, Sweden
J Leukoc Biol 83:31-8. 2008..Together, these results suggest a new mechanism for the anti-rheumatic effects of gold salts in RA and the potential of drugs, which interfere with intracellular HMGB1 transport mechanisms, as novel agents to treat RA... - Microparticles stimulate the synthesis of prostaglandin E(2) via induction of cyclooxygenase 2 and microsomal prostaglandin E synthase 1Astrid Jungel
Zurich Center of Integrative Human Physiology, and University Hospital Zurich, Zurich, Switzerland
Arthritis Rheum 56:3564-74. 2007..The goal of these studies was to elucidate the mechanisms by which microparticles activate synovial fibroblasts to express a proinflammatory phenotype... - Fulfilling Koch's postulates of autoimmunity: anti-NR2 antibodies in mice and menDavid S Pisetsky
Arthritis Rheum 54:2349-52. 2006 - The induction of matrix metalloproteinase and cytokine expression in synovial fibroblasts stimulated with immune cell microparticlesJörg H W Distler
Center of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, CH 8091 Zurich, Switzerland
Proc Natl Acad Sci U S A 102:2892-7. 2005..These data provide evidence for a novel mechanism by which vesicles derived from activated or apoptotic immune cells can promote the destructive activity of synovial fibroblasts in rheumatoid arthritis... - Screening the genome for rheumatoid arthritis susceptibility genes: a replication study and combined analysis of 512 multicase familiesDamini Jawaheer
Center for Genomics and Human Genetics, Manhasset, New York 11030, USA
Arthritis Rheum 48:906-16. 2003..CONCLUSION: These results provide a rational basis for pursuing high-density linkage and association studies of RA in several regions outside of the HLA region, particularly on chromosomes 1p, 1q, and 18q...