C V Nicchitta

Summary

Affiliation: Duke University Medical Center
Country: USA

Publications

  1. pmc Come forth CD1d: Hsp110 in the regulation of intestinal epithelial CD1d expression
    Christopher V Nicchitta
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Clin Invest 112:646-8. 2003
  2. ncbi request reprint The immunological properties of endoplasmic reticulum chaperones: a conflict of interest?
    C V Nicchitta
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Essays Biochem 36:15-25. 2000
  3. ncbi request reprint Re-evaluating the role of heat-shock protein-peptide interactions in tumour immunity
    Christopher V Nicchitta
    Department of Cell Biology, Duke University Medical Centre, Durham, North Carolina 27710, USA
    Nat Rev Immunol 3:427-32. 2003
  4. pmc The messenger and the message: gp96 (GRP94)-peptide interactions in cellular immunity
    Christopher V Nicchitta
    Department of Cell Biology, Duke University Medical Center, Box 3709, Durham, NC 27710, USA
    Cell Stress Chaperones 9:325-31. 2004
  5. ncbi request reprint A platform for compartmentalized protein synthesis: protein translation and translocation in the ER
    Christopher V Nicchitta
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Opin Cell Biol 14:412-6. 2002
  6. ncbi request reprint Biochemical, cell biological and immunological issues surrounding the endoplasmic reticulum chaperone GRP94/gp96
    C V Nicchitta
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Opin Immunol 10:103-9. 1998
  7. pmc Regulation of the ribosome-membrane junction at early stages of presecretory protein translocation in the mammalian endoplasmic reticulum
    C V Nicchitta
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Cell Biol 139:1697-708. 1997
  8. ncbi request reprint To find the road traveled to tumor immunity: the trafficking itineraries of molecular chaperones in antigen-presenting cells
    B Berwin
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Traffic 2:690-7. 2001
  9. ncbi request reprint Virally induced lytic cell death elicits the release of immunogenic GRP94/gp96
    B Berwin
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 276:21083-8. 2001
  10. ncbi request reprint Ribosome exchange revisited: a mechanism for translation-coupled ribosome detachment from the ER membrane
    M D Potter
    Dept of Cell Biology, Duke University Medical Center, Box 3709 DUMC, Durham, NC 27710, USA
    Trends Cell Biol 11:112-5. 2001

Research Grants

  1. The Molecular Mechanism of GRP94 Function
    Christopher Nicchitta; Fiscal Year: 2005
  2. Mechanism of Chaperone-Mediated Tumor Rejection
    Christopher Nicchitta; Fiscal Year: 2007
  3. Regulation of mRNA Partitioning to the Endoplasmic Reticulum
    Christopher Nicchitta; Fiscal Year: 2009
  4. Regulation of mRNA Partitioning to the Endoplasmic Reticulum
    Christopher V Nicchitta; Fiscal Year: 2010
  5. MOLECULAR MECHANISM OF PROTEIN TRANSLOCATION
    Christopher Nicchitta; Fiscal Year: 2001
  6. MOLECULAR MECHANISM OF GRP94 FUNCTION
    Christopher Nicchitta; Fiscal Year: 2000
  7. Regulation of mRNA Partitioning to the Endoplasmic Reticulum
    Christopher Nicchitta; Fiscal Year: 2009

Collaborators

  • JACK KEENE
  • Chuan-Yuan Li
  • T Zheng
  • H Yu
  • M P Sheetz
  • I Toyoshima
  • J Yewdell
  • Samuel B Stephens
  • Julie C Baker-Lepain
  • Brent Berwin
  • Rachel S Lerner
  • Jason C Maynard
  • B Berwin
  • Robyn C Reed
  • Patrick J Lager
  • Brook Pyhtila
  • Avital Lev
  • Rebecca D Dodd
  • Feixia Chu
  • Mary C Reedy
  • Shanling Liu
  • Meredith F N Rosser
  • Karen L Soldano
  • Marcella Sarzotti
  • Justin P Hart
  • Salvatore V Pizzo
  • Matthew D Potter
  • M D Potter
  • Christopher B Newgard
  • Trang Pham
  • Helen B Rankin
  • Eric P Spana
  • Angela Jockheck-Clark
  • Peniel Dimberu
  • Nir Netzer
  • Yoram Reiter
  • Damien Zanker
  • Michael F Princiotta
  • Kazuyo Takeda
  • Elizabeth Waffarn
  • Didier Picard
  • James Gibbs
  • Weisan Chen
  • Jack R Bennink
  • BROOK M PYHTILA
  • Len Neckers
  • Gabriela Chiosis
  • Alma L Burlingame
  • Jiangao Zhu
  • John Kirkpatrick
  • Fang Li
  • He Wang
  • Zhonghui Yang
  • Joseph W Brewer
  • Takashi Kon
  • Yiting Cao
  • Brian M Trotta
  • Megan R Marshall
  • Daniel T Gewirth
  • Stuart Rice
  • Arif Jivan
  • Cecilia Gass
  • Steven R Post
  • Jeffrey P Baker
  • Robert M Seiser
  • Timothy A Fields
  • K G Brinker
  • M F N Rosser
  • R C Reed
  • R M Seiser

Detail Information

Publications35

  1. pmc Come forth CD1d: Hsp110 in the regulation of intestinal epithelial CD1d expression
    Christopher V Nicchitta
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Clin Invest 112:646-8. 2003
    ..Might this abundant chaperone serve an autocrine function in the regulation of CD1d expression?..
  2. ncbi request reprint The immunological properties of endoplasmic reticulum chaperones: a conflict of interest?
    C V Nicchitta
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Essays Biochem 36:15-25. 2000
    ..Perspectives on the regulation of and interplay between the peptide binding and chaperone activity of GRP94 and calreticulin are discussed...
  3. ncbi request reprint Re-evaluating the role of heat-shock protein-peptide interactions in tumour immunity
    Christopher V Nicchitta
    Department of Cell Biology, Duke University Medical Centre, Durham, North Carolina 27710, USA
    Nat Rev Immunol 3:427-32. 2003
    ..However, recent findings, including new data on the cell biology of peptide generation and trafficking, have called into question the specificity of tumour rejection that is induced by HSPs...
  4. pmc The messenger and the message: gp96 (GRP94)-peptide interactions in cellular immunity
    Christopher V Nicchitta
    Department of Cell Biology, Duke University Medical Center, Box 3709, Durham, NC 27710, USA
    Cell Stress Chaperones 9:325-31. 2004
    ..Recent insights into the pathways for peptide generation now allow this hypothesis to be critically examined, which is the subject of this review...
  5. ncbi request reprint A platform for compartmentalized protein synthesis: protein translation and translocation in the ER
    Christopher V Nicchitta
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Opin Cell Biol 14:412-6. 2002
    ..A hypothesis identifying the endoplasmic reticulum as a site of mRNA localization and compartmentalized protein synthesis has been suggested...
  6. ncbi request reprint Biochemical, cell biological and immunological issues surrounding the endoplasmic reticulum chaperone GRP94/gp96
    C V Nicchitta
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Curr Opin Immunol 10:103-9. 1998
    ..Insights into the structural basis of peptide binding to GRP94 have been obtained and the role of the transporter for antigen presentation in defining the GRP94-bound peptide composition has been determined...
  7. pmc Regulation of the ribosome-membrane junction at early stages of presecretory protein translocation in the mammalian endoplasmic reticulum
    C V Nicchitta
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Cell Biol 139:1697-708. 1997
    ..A model describing a potential mode of regulation of the ribosome-membrane junction by the nascent chain is presented...
  8. ncbi request reprint To find the road traveled to tumor immunity: the trafficking itineraries of molecular chaperones in antigen-presenting cells
    B Berwin
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Traffic 2:690-7. 2001
    ....
  9. ncbi request reprint Virally induced lytic cell death elicits the release of immunogenic GRP94/gp96
    B Berwin
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 276:21083-8. 2001
    ..These data identify GRP94 as an antigenic component released upon pathological, but not apoptotic, cell death and provide an assay system for the identification of cellular components of related activity...
  10. ncbi request reprint Ribosome exchange revisited: a mechanism for translation-coupled ribosome detachment from the ER membrane
    M D Potter
    Dept of Cell Biology, Duke University Medical Center, Box 3709 DUMC, Durham, NC 27710, USA
    Trends Cell Biol 11:112-5. 2001
    ..Our model emphasizes a role for the conformation of the large ribosomal subunit in the regulation of membrane affinity and provides a mechanism for translation-coupled ribosome release...
  11. ncbi request reprint Pathways for compartmentalizing protein synthesis in eukaryotic cells: the template-partitioning model
    Christopher V Nicchitta
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Biochem Cell Biol 83:687-95. 2005
    ..In this review, key findings on this topic are discussed, and the template-partitioning model, describing a hypothetical mechanism for RNA partitioning in the eukaryotic cell, is proposed...
  12. pmc Divergent regulation of protein synthesis in the cytosol and endoplasmic reticulum compartments of mammalian cells
    Samuel B Stephens
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 19:623-32. 2008
    ..These findings identify a role for the ER in global protein synthesis, and they suggest models where compartmentalization of the tRNA acylation/deacylation cycle contributes to the regulation of global protein synthesis rates...
  13. ncbi request reprint Transfer of GRP94(Gp96)-associated peptides onto endosomal MHC class I molecules
    B Berwin
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Traffic 3:358-66. 2002
    ....
  14. pmc mRNA translation is compartmentalized to the endoplasmic reticulum following physiological inhibition of cap-dependent translation
    Rachel S Lerner
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 12:775-89. 2006
    ..In total, these data demonstrate that ribosome and mRNA release from the ER is regulated independent of translation termination and identify the ER as a privileged site for protein synthesis...
  15. pmc Stable ribosome binding to the endoplasmic reticulum enables compartment-specific regulation of mRNA translation
    Samuel B Stephens
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 16:5819-31. 2005
    ..These studies demonstrate that ribosome release from the ER is termination independent and identify new and unexpected roles for the ER compartment in the translational response to induction of the unfolded protein response...
  16. ncbi request reprint Endoplasmic reticulum-bound ribosomes reside in stable association with the translocon following termination of protein synthesis
    Matthew D Potter
    Department of Cell Biology, Box 3709, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:23314-20. 2002
    ..On the basis of these data, we propose that in situ, ribosomes reside in association with the translocon throughout the cycle of protein synthesis, with membrane release occurring upon translation of proteins lacking topogenic signals...
  17. pmc Characterization of kinectin, a kinesin-binding protein: primary sequence and N-terminal topogenic signal analysis
    H Yu
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Mol Biol Cell 6:171-83. 1995
    ..Thus, the kinectin cDNA encodes a cytoplasmic-oriented integral membrane protein that binds kinesin and is likely to be a coiled-coil dimer...
  18. pmc Partitioning and translation of mRNAs encoding soluble proteins on membrane-bound ribosomes
    Rachel S Lerner
    Departments of Cell Biology and Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 9:1123-37. 2003
    ....
  19. doi request reprint Analysis of mRNA partitioning between the cytosol and endoplasmic reticulum compartments of mammalian cells
    Samuel B Stephens
    Department of Cell Biology, Duke University Medical Center, Durham, NC, USA
    Methods Mol Biol 419:197-214. 2008
    ..In this contribution, we briefly review the literature describing the subcellular partitioning patterns of mRNAs and present a detailed methodology for studying this fundamental, yet poorly understood process...
  20. pmc Scavenger receptor-A mediates gp96/GRP94 and calreticulin internalization by antigen-presenting cells
    Brent Berwin
    Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA
    EMBO J 22:6127-36. 2003
    ..In addition, macrophages derived from SR-A-/- mice were substantially impaired in gp96 binding and uptake. These data identify new roles for SR-A in the regulation of cellular responses to heat shock proteins...
  21. pmc Signal sequence- and translation-independent mRNA localization to the endoplasmic reticulum
    Brook Pyhtila
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    RNA 14:445-53. 2008
    ..Combined, these data indicate that the mRNA localization to the ER can be conferred independent of the signal sequence/SRP pathway and suggest that mRNA localization to the ER may utilize cis-encoded targeting information...
  22. ncbi request reprint Cutting edge: CD91-independent cross-presentation of GRP94(gp96)-associated peptides
    Brent Berwin
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    J Immunol 168:4282-6. 2002
    ..These data identify a CD91-independent, GRP94 internalization pathway that functions in peptide Ag re-presentation...
  23. ncbi request reprint In vitro and tissue culture methods for analysis of translation initiation on the endoplasmic reticulum
    Samuel B Stephens
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina, USA
    Methods Enzymol 431:47-60. 2007
    ..In the following chapter, we provide detailed experimental methods to study protein synthesis initiation on the ER membrane...
  24. ncbi request reprint ISO: a critical evaluation of the role of peptides in heat shock/chaperone protein-mediated tumor rejection
    Julie C Baker-Lepain
    Department of Cell Biology, Box 3709 Duke University Medical Center, Durham, NC 27710, USA
    Curr Opin Immunol 15:89-94. 2003
  25. pmc Gp93, the Drosophila GRP94 ortholog, is required for gut epithelial homeostasis and nutrient assimilation-coupled growth control
    Jason C Maynard
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Dev Biol 339:295-306. 2010
    ....
  26. pmc GRP94 (gp96) and GRP94 N-terminal geldanamycin binding domain elicit tissue nonrestricted tumor suppression
    Julie C Baker-Lepain
    Department of Cell Biology, Duke University Medical Center, 366 Nanaline H Duke, Durham, NC 27710, USA
    J Exp Med 196:1447-59. 2002
    ..Based on these findings, we propose that GRP94-elicited tumor suppression can occur independent of the GRP94 tissue of origin and suggest a primary role for GRP4 natural adjuvant function in antitumor immune responses...
  27. ncbi request reprint Glucose-regulated protein 94/glycoprotein 96 elicits bystander activation of CD4+ T cell Th1 cytokine production in vivo
    Julie C Baker-Lepain
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    J Immunol 172:4195-203. 2004
    ....
  28. ncbi request reprint Structure of the N-terminal domain of GRP94. Basis for ligand specificity and regulation
    Karen L Soldano
    Departments of Biochemistry and Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 278:48330-8. 2003
    ..This continuation of the beta sheet past the charged domain suggests a structural basis for the association of the N-terminal and middle domains of the full-length chaperone...
  29. ncbi request reprint Enhancement of cancer radiation therapy by use of adenovirus-mediated secretable glucose-regulated protein 94/gp96 expression
    Shanling Liu
    Department of Radiation Oncology, Duke University Medical Center, Durham, NC 27710, USA
    Cancer Res 65:9126-31. 2005
    ..Our results indicate that combined AdsGRP94-based immunotherapy and radiation therapy may be a potentially effective strategy for cancer treatment...
  30. ncbi request reprint GRP94/gp96 elicits ERK activation in murine macrophages. A role for endotoxin contamination in NF-kappa B activation and nitric oxide production
    Robyn C Reed
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 278:31853-60. 2003
    ..These results are discussed with respect to current understanding of the contributions of endotoxin and heat shock/chaperone proteins to the stimulation of innate immune responses...
  31. ncbi request reprint Adenosine nucleotides and the regulation of GRP94-client protein interactions
    Meredith F N Rosser
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    Biochemistry 43:8835-45. 2004
    ..On the basis of these data, we propose that structural maturation of the client protein substrate, rather than ATP binding or hydrolysis, serves as the primary signal for dissociation of GRP94-client protein complexes...
  32. ncbi request reprint GRP94-associated enzymatic activities. Resolution by chromatographic fractionation
    Robyn C Reed
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:25082-9. 2002
    ..These results are discussed with respect to current models for GRP94 function and the role of such co-purifying (poly)peptides in the generation of GRP94-dependent cellular immune responses...
  33. pmc Identification of novel quaternary domain interactions in the Hsp90 chaperone, GRP94
    Feixia Chu
    Mass Spectrometry Facility, University of California, San Francisco, California 94143, USA
    Protein Sci 15:1260-9. 2006
    ..These results identify a compact, intertwined quaternary conformation of native GRP94 and suggest that intersubunit N+M interactions are integral to the structural biology of Hsp90...
  34. ncbi request reprint The DRiP hypothesis decennial: support, controversy, refinement and extension
    Jonathan W Yewdell
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892 0440, USA
    Trends Immunol 27:368-73. 2006
    ....
  35. pmc The exception that reinforces the rule: crosspriming by cytosolic peptides that escape degradation
    Avital Lev
    Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Immunity 28:787-98. 2008
    ....

Research Grants25

  1. The Molecular Mechanism of GRP94 Function
    Christopher Nicchitta; Fiscal Year: 2005
    ..abstract_text> ..
  2. Mechanism of Chaperone-Mediated Tumor Rejection
    Christopher Nicchitta; Fiscal Year: 2007
    ..abstract_text> ..
  3. Regulation of mRNA Partitioning to the Endoplasmic Reticulum
    Christopher Nicchitta; Fiscal Year: 2009
    ..By understanding these mechanisms, we hope to identify new targets for therapeutic intervention. ..
  4. Regulation of mRNA Partitioning to the Endoplasmic Reticulum
    Christopher V Nicchitta; Fiscal Year: 2010
    ..By understanding these mechanisms, we hope to identify new targets for therapeutic intervention. ..
  5. MOLECULAR MECHANISM OF PROTEIN TRANSLOCATION
    Christopher Nicchitta; Fiscal Year: 2001
    ..The investigator proposes a reexamination of current models and assumptions concerning the nature and role of the association of ribosomes and the nascent chain with the ER membrane. ..
  6. MOLECULAR MECHANISM OF GRP94 FUNCTION
    Christopher Nicchitta; Fiscal Year: 2000
    ..To test whether dimerization is necessary for function, mutations in the assembly domain that block dimerization will be assessed by in vitro studies of peptide binding and in vivo studies of protein assembly and secretion. ..
  7. Regulation of mRNA Partitioning to the Endoplasmic Reticulum
    Christopher Nicchitta; Fiscal Year: 2009
    ..By understanding these mechanisms, we hope to identify new targets for therapeutic intervention. ..