Daniel J Lew

Summary

Affiliation: Duke University Medical Center
Country: USA

Publications

  1. Chiou J, Balasubramanian M, Lew D. Cell Polarity in Yeast. Annu Rev Cell Dev Biol. 2017;33:77-101 pubmed publisher
    ..Species-specific pathways regulate the timing of polarization during the cell cycle, as well as the location and number of polarity sites. ..
  2. request reprint
    Lew D. Cell-cycle checkpoints that ensure coordination between nuclear and cytoplasmic events in Saccharomyces cerevisiae. Curr Opin Genet Dev. 2000;10:47-53 pubmed
    ..Thus, specific cell-cycle regulators are well-placed to monitor whether a cell has formed a bud and whether a daughter nucleus has been delivered accurately to the bud following mitosis. ..
  3. Woods B, Kuo C, Wu C, Zyla T, Lew D. Polarity establishment requires localized activation of Cdc42. J Cell Biol. 2015;211:19-26 pubmed publisher
    ..Here we show that Cdc42 activation must be localized for successful polarity establishment, supporting local activation rather than local delivery as the dominant mechanism in this system. ..
  4. Kang H, Lew D. How do cells know what shape they are?. Curr Genet. 2017;63:75-77 pubmed publisher
    ..Together, these findings suggest a model for how cells may monitor aspects of their own shape to influence cell behavior. ..
  5. Woods B, LAI H, Wu C, Zyla T, Savage N, Lew D. Parallel Actin-Independent Recycling Pathways Polarize Cdc42 in Budding Yeast. Curr Biol. 2016;26:2114-26 pubmed publisher
    ..We suggest that GAP activity cooperates with the GDI to counteract the dissipative effect of a previously unappreciated pathway whereby GTP-Cdc42 escapes from the polarity site through the cytoplasm. ..
  6. Kuo C, Savage N, Chen H, Wu C, Zyla T, Lew D. Inhibitory GEF phosphorylation provides negative feedback in the yeast polarity circuit. Curr Biol. 2014;24:753-9 pubmed publisher
    ..These findings reveal a mechanism for negative feedback and suggest that the function of negative feedback via GEF inhibition is to buffer the level of Cdc42 at the polarity site. ..
  7. Kang H, Tsygankov D, Lew D. Sensing a bud in the yeast morphogenesis checkpoint: a role for Elm1. Mol Biol Cell. 2016;27:1764-75 pubmed publisher
    ..Moreover, recruitment of Elm1 is responsive to bud emergence. Our findings suggest that Elm1 plays a key role in sensing bud emergence. ..
  8. McClure A, Wu C, Johnson S, Lew D. Imaging Polarization in Budding Yeast. Methods Mol Biol. 2016;1407:13-23 pubmed publisher
    ....

Collaborators

Detail Information

Publications8

  1. Chiou J, Balasubramanian M, Lew D. Cell Polarity in Yeast. Annu Rev Cell Dev Biol. 2017;33:77-101 pubmed publisher
    ..Species-specific pathways regulate the timing of polarization during the cell cycle, as well as the location and number of polarity sites. ..
  2. request reprint
    Lew D. Cell-cycle checkpoints that ensure coordination between nuclear and cytoplasmic events in Saccharomyces cerevisiae. Curr Opin Genet Dev. 2000;10:47-53 pubmed
    ..Thus, specific cell-cycle regulators are well-placed to monitor whether a cell has formed a bud and whether a daughter nucleus has been delivered accurately to the bud following mitosis. ..
  3. Woods B, Kuo C, Wu C, Zyla T, Lew D. Polarity establishment requires localized activation of Cdc42. J Cell Biol. 2015;211:19-26 pubmed publisher
    ..Here we show that Cdc42 activation must be localized for successful polarity establishment, supporting local activation rather than local delivery as the dominant mechanism in this system. ..
  4. Kang H, Lew D. How do cells know what shape they are?. Curr Genet. 2017;63:75-77 pubmed publisher
    ..Together, these findings suggest a model for how cells may monitor aspects of their own shape to influence cell behavior. ..
  5. Woods B, LAI H, Wu C, Zyla T, Savage N, Lew D. Parallel Actin-Independent Recycling Pathways Polarize Cdc42 in Budding Yeast. Curr Biol. 2016;26:2114-26 pubmed publisher
    ..We suggest that GAP activity cooperates with the GDI to counteract the dissipative effect of a previously unappreciated pathway whereby GTP-Cdc42 escapes from the polarity site through the cytoplasm. ..
  6. Kuo C, Savage N, Chen H, Wu C, Zyla T, Lew D. Inhibitory GEF phosphorylation provides negative feedback in the yeast polarity circuit. Curr Biol. 2014;24:753-9 pubmed publisher
    ..These findings reveal a mechanism for negative feedback and suggest that the function of negative feedback via GEF inhibition is to buffer the level of Cdc42 at the polarity site. ..
  7. Kang H, Tsygankov D, Lew D. Sensing a bud in the yeast morphogenesis checkpoint: a role for Elm1. Mol Biol Cell. 2016;27:1764-75 pubmed publisher
    ..Moreover, recruitment of Elm1 is responsive to bud emergence. Our findings suggest that Elm1 plays a key role in sensing bud emergence. ..
  8. McClure A, Wu C, Johnson S, Lew D. Imaging Polarization in Budding Yeast. Methods Mol Biol. 2016;1407:13-23 pubmed publisher
    ....