David E Hinton
Affiliation: Duke University Medical Center
- Resolving mechanisms of toxicity while pursuing ecotoxicological relevance?David E Hinton
Laboratory of Molecular Aquatic Toxicology, Division of Environmental Sciences and Policy, Nicholas School of the Environment and Earth Sciences, Duke University Durham, NC 277 8 0328, USA
Mar Pollut Bull 51:635-48. 2005..We review studies resolving mechanisms of toxicity and discuss ways to address biocomplexity, mixtures of contaminants, and the need to relate individual level responses to populations and communities...
- Gene expression throughout a vertebrate's embryogenesisGoran Bozinovic
Department of Environmental and Molecular Toxicology, North Carolina State University, Raleigh, 27695 7633, USA
BMC Genomics 12:132. 2011..This study presents statistical analyses of gene expression during all 40 developmental stages in the teleost Fundulus heteroclitus using four biological replicates per stage...
- Non invasive high resolution in vivo imaging of alpha-naphthylisothiocyanate (ANIT) induced hepatobiliary toxicity in STII medakaRon Hardman
Duke University, Nicholas School of the Environment and Earth Sciences, Durham, NC 27708, USA
Aquat Toxicol 86:20-37. 2008....
- Differential developmental toxicity of naphthoic acid isomers in medaka (Oryzias latipes) embryosMichael W Carney
Laboratory of Molecular Aquatic Toxicology, Division of Environmental Sciences and Policy, Nicholas School of the Environment and Earth Sciences, Duke University, Durham, NC 27708 0328, United States
Mar Pollut Bull 57:255-66. 2008..Our results demonstrate the use of medaka embryos for toxicity analysis thereby achieving REACH objectives for the reduction of adult animal testing in toxicity evaluations...
- Analysis of medaka cytochrome P450 3A homotropic and heterotropic cooperativitySeth W Kullman
Nicholas School of the Environment and Earth Sciences, Duke University, Box 90328, Durham, NC 27708, USA
Mar Environ Res 58:469-73. 2004..Given the role of CYP3A in maintaining the homeostatic balance for numerous endobiotics, enzymatic activation/inhibition by endocrine disruptors (EDCs) represents a putative (non-genomic) mechanism for endocrine disruption...
- Uptake of silver nanoparticles and toxicity to early life stages of Japanese medaka (Oryzias latipes): effect of coating materialsKevin W H Kwok
Nicholas School of the Environment and Center for the Environmental Implications of NanoTechnology, Duke University Durham, NC 27708 0328, USA
Aquat Toxicol 120:59-66. 2012..In conclusion, AgNPs is a source of toxic Ag ions, while itself contribute partially to its toxicity to fish, and which interact with skin surface and were taken up via the gills...
- Paralogous vitamin D receptors in teleosts: transition of nuclear receptor functionDeanna L Howarth
Integrated Toxicology and Environmental Health Program, Nicholas School of the Environment and Earth Sciences, Duke University, Durham, North Carolina 27708, USA
Endocrinology 149:2411-22. 2008..These results suggest that in mVDRalpha and VDRbeta have undergone a functional divergence through a process of sub- and/or neofunctionalization of VDR nuclear receptor gene pairs...
- Functional characterization of medaka CYP3A38 and CYP3A40: kinetics and catalysis by expression in a recombinant baculovirus systemShosaku Kashiwada
Division of Environmental Sciences and Policy, Nicholas School of the Environment and Earth Sciences, Duke University, Box 90328, LSRC Research Drive, Durham, NC 27708 0328, USA
Comp Biochem Physiol C Toxicol Pharmacol 141:338-48. 2005..We suggest that differences in kinetics and catalytic activities are a result of amino acid substitutions in SRS regions 1, 3 and 5 within the CYP3A38 and CYP3A40 protein sequence...
- An in vivo look at vertebrate liver architecture: three-dimensional reconstructions from medaka (Oryzias latipes)Ron C Hardman
Duke University, Nicholas School of the Environment and Earth Sciences, Durham, North Carolina 27708, USA
Anat Rec (Hoboken) 290:770-82. 2007....
- Dynamic gene expression changes precede dioxin-induced liver pathogenesis in medaka fishDavid C Volz
Integrated Toxicology Program and Nicholas School of the Environment and Earth Sciences, Duke University, Durham, North Carolina 27708, USA
Toxicol Sci 89:524-34. 2006....
- 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) induces organ- specific differential gene expression in male Japanese medaka (Oryzias latipes)David C Volz
Integrated Toxicology Program and Nicholas School of the Environment and Earth Sciences, Duke University, Durham, North Carolina 27708 0328, USA
Toxicol Sci 85:572-84. 2005..Based on these data, unique liver-specific and testis-specific mRNA-level targets in male medaka were identified as promising biomarkers of acute TCDD-induced toxicity...
- Protective response of the Ah receptor to ANIT-induced biliary epithelial cell toxicity in see-through medakaDavid C Volz
Integrated Toxicology Program and Nicholas School of the Environment and Earth Sciences, Duke University, Durham, North Carolina 27708
Toxicol Sci 102:262-77. 2008..Given that bile acids and pigments are present in mammalian and fish liver, these studies collectively suggest that bile-induced AHR activation may be conserved between teleosts and rodents...
- Comparisons of polychromatic and monochromatic UV-based treatments of bisphenol-A in water via toxicity assessmentsPei Jen Chen
National Health and Environmental Effects Research Laboratory, EPA, RTP, NC, USA
Chemosphere 68:1041-9. 2007..Furthermore, the UV/H2O2 process was effective for reducing embryo toxicity of BPA, but resulted in the production of acidic intermediates, causing acute toxicity and delayed hatching in some medaka embryos...
- Biomonitoring recycled water in the Santa Ana River Basin in southern CaliforniaXin Deng
Department of Environmental Sciences, University of California, Riverside, California, USA
J Toxicol Environ Health A 71:109-18. 2008..These results demonstrate that OR Japanese medaka may be a sensitive strain as an on-line monitor to predict potential impacts of water quality, but further studies are needed to elicit causative agents within the water mixture...
- Estrogenic modulation of CYP3A38, CYP3A40, and CYP19 in mature male medaka (Oryzias latipes)Shosaku Kashiwada
National Institute for Environmental Studies, Tsukuba, Ibaraki 305 8506, Japan
Comp Biochem Physiol C Toxicol Pharmacol 145:370-8. 2007..Estrogenic exposure induced Vtg production, suppressed CYP3A protein production, downregulated TE metabolism, and enhanced CYP19 activity. Serum E2 endogenously induced by CYP19 could contribute to Vtg induction in male medaka...
- Biological assessments of a mixture of endocrine disruptors at environmentally relevant concentrations in water following UV/H2O2 oxidationPei Jen Chen
Department of Civil and Environmental Engineering, Duke University, USA
Sci Total Environ 376:18-26. 2007....
- An artificial fertilization method with the Japanese medaka: implications in early life stage bioassays and solvent toxicityMiguel González-Doncel
Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California Davis, Davis, CA 95616, USA
Ecotoxicol Environ Saf 69:95-103. 2008..Further research is needed to investigate if using this exposure methodology for chemicals with more specific modes of action may result in increased sensitivities...
- Vulnerable windows for developmental ethanol toxicity in the Japanese medaka fish (Oryzias latipes)Sharon L Oxendine
Curriculum in Toxicology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States
Aquat Toxicol 80:396-404. 2006..In general, these data suggest that critical periods for heightened sensitivity to developmental EtOH exposure may vary according to the specific endpoint used to assess toxicity...
- Age-dependent in situ hepatic and gill CYP1A activity in the see-through medaka (Oryzias latipes)Shosaku Kashiwada
National Institute for Environmental Studies, Tsukuba, Japan
Comp Biochem Physiol C Toxicol Pharmacol 145:96-102. 2007..This intrinsic activity declined with growth and explained the high constant EROD activity at 1-week post-hatch stage...
- Molecular cloning of rainbow trout (Oncorhynchus mykiss) eggshell zona radiata protein complementary DNA: mRNA expression in 17beta-estradiol- and nonylphenol-treated fishAugustine Arukwe
Great Lakes Institute for Environmental Research, University of Windor, 401 Sunset Avenue, Windsor, Ontario N9B 3P4, Canada
Comp Biochem Physiol B Biochem Mol Biol 132:315-26. 2002..The differential time-dependent transcriptional and translational expression and use of Zrp, ER and Vtg as sensitive biomarkers in environmental monitoring of endocrine disrupters in fish is discussed...
- Effects of husbandry practices, gender, and normal physiological variation on growth and reproduction of Japanese medaka, Oryzias latipesCorrine R Davis
Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, One Shields Ave, Davis, CA 95616, USA
Aquat Toxicol 60:185-201. 2002..e. carcinogenesis and endocrine disruption studies. The high degree of individual variation among normal females and daily fluctuations in organ weights should also be considered in study design...
- Utilizing in vivo nuclear magnetic resonance spectroscopy to study sublethal stress in aquatic organismsMark R Viant
Department of Environmental Toxicology, University of California, Davis 95616, USA
Mar Environ Res 54:553-7. 2002..Advantages and disadvantages of in vivo NMR will be discussed...
- Effects of dietary exposure to the pyrethroid pesticide esfenvalerate on medaka (Oryzias latipes)Ingeborg Werner
Sehool of Veterinary Medicine, Department of Anatomy, Physiology and Cell Biology, University of California, Davis 95616, USA
Mar Environ Res 54:609-14. 2002..Possible effects of dietary esfenvalerate were seen on fertilization and hatching success and the number of non-viable larvae. Expression of hsp60 and hsp90 showed a dose-dependent response pattern...
- Stage sensitivity of medaka (Oryzias latipes) eggs and embryos to permethrinMiguel González-Doncel
Centro Nacional de Sanidad Ambiental, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain
Aquat Toxicol 62:255-68. 2003..The approach taken herein may better reflect environmental risk conditions than assays limited to exposure of embryonated eggs...
- Influence of water hardening of the chorion on cadmium accumulation in medaka (Oryzias latipes) eggsMiguel González-Doncel
Centro Nacional de Sanidad Ambiental, Instituto de Salud Carlos III, 28220 Madrid, Majadahonda, Spain
Chemosphere 52:75-83. 2003..Also, sensitive methods to localize Cd(2+) and to delineate the transfer from the chorion to the embryo are needed...
- ras oncogene mutations in diethylnitrosamine-induced hepatic tumors in medaka (Oryzias latipes), a teleost fishZi Liu
Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
Mutat Res 539:43-53. 2003..A ras mutation incidence of 30% is similar to that reported in mammalian species exposed to DEN. While mutations at codon 12 have previously been reported, the present study is the first in vivo report of ras point mutations at codon 16...
- Metabolic changes in Japanese medaka (Oryzias latipes) during embryogenesis and hypoxia as determined by in vivo 31P NMRChristopher A Pincetich
Department of Environmental Toxicology, College of Agricultural and Environmental Sciences, University of California, Davis, 95616 8588, USA
Comp Biochem Physiol C Toxicol Pharmacol 140:103-13. 2005....
- Toxic actions of dinoseb in medaka (Oryzias latipes) embryos as determined by in vivo 31P NMR, HPLC-UV and 1H NMR metabolomicsMark R Viant
School of Biosciences, The University of Birmingham, Edgbaston, UK
Aquat Toxicol 76:329-42. 2006....
- Adapting the medaka embryo assay to a high-throughput approach for developmental toxicity testingSharon L Oxendine
Curriculum in Toxicology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Neurotoxicology 27:840-5. 2006....
- Biological assessment of bisphenol A degradation in water following direct photolysis and UV advanced oxidationPei Jen Chen
Department of Civil and Environmental Engineering, Duke University, United States
Chemosphere 65:1094-102. 2006..Furthermore, the UV/H(2)O(2) AOP was effective for reducing larval lethality in treated BPA solutions, suggesting BPA degradation occurred and that the degradation process did not result in the production of acutely toxic intermediates...
- Effects of handling on heat shock protein expression in rainbow trout (Oncorhynchus mykiss)Barbara Shayne Washburn
Department of Biological Sciences, University of Texas, El Paso 79968, USA
Environ Toxicol Chem 21:557-60. 2002..We found no effect of any handling regimen on the induction of HSPs. These findings suggest that the capture and transport of fish for environmental monitoring purposes should not interfere with the use of stress proteins as biomarkers...
- Enhanced Image Analysis of a Transparent Fish ModelDavid Hinton; Fiscal Year: 2006..Thus, in this application, we will establish specific molecular imaging technologies to study organ system growth and development and to enhance this model's potential to study effects of human disease. ..
- Aquatic Animal Models for Human Health StudiesDavid Hinton; Fiscal Year: 2007..Among the expected outcomes of the meeting include improved use of resources and stimulation of collaborations. ..