B F Haynes

Summary

Affiliation: Duke University Medical Center
Country: USA

Publications

  1. doi request reprint IGHV1-69 B cell chronic lymphocytic leukemia antibodies cross-react with HIV-1 and hepatitis C virus antigens as well as intestinal commensal bacteria
    Kwan Ki Hwang
    Duke Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina, United States of America
    PLoS ONE 9:e90725. 2014
  2. doi request reprint Progress in HIV-1 vaccine development
    Barton F Haynes
    Duke Human Vaccine Institute, Duke University Medical Center, Durham, NC 27710, USA
    Curr Opin HIV AIDS 8:326-32. 2013
  3. pmc B-cell-lineage immunogen design in vaccine development with HIV-1 as a case study
    Barton F Haynes
    Duke Human Vaccine Institute, Department of Medicine, Duke University School of Medicine, Durham, North Carolina, USA
    Nat Biotechnol 30:423-33. 2012
  4. pmc Immune-correlates analysis of an HIV-1 vaccine efficacy trial
    Barton F Haynes
    Duke University Human Vaccine Institute and the Center for HIV AIDS Vaccine Immunology, Duke University School of Medicine, Durham, NC 27710, USA
    N Engl J Med 366:1275-86. 2012
  5. ncbi request reprint The human thymus during aging
    B F Haynes
    Department of Medicine, Duke University Arthritis Center, Duke Human Vaccine Institute, Duke University School of Medicine, Durham, NC, USA
    Immunol Res 22:253-61. 2000
  6. pmc Antibody polyspecificity and neutralization of HIV-1: a hypothesis
    Barton F Haynes
    Duke Human Vaccine Institute, Duke University School of Medicine, Durham NC 27710, USA
    Hum Antibodies 14:59-67. 2005
  7. ncbi request reprint Analysis of HIV-1 subtype B third variable region peptide motifs for induction of neutralizing antibodies against HIV-1 primary isolates
    Barton F Haynes
    Department of Medicine and Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA
    Virology 345:44-55. 2006
  8. ncbi request reprint The role of the thymus in immune reconstitution in aging, bone marrow transplantation, and HIV-1 infection
    B F Haynes
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Annu Rev Immunol 18:529-60. 2000
  9. ncbi request reprint The human thymus. A chimeric organ comprised of central and peripheral lymphoid components
    B F Haynes
    Department of Medicine, Duke University School of Medicine, Duke Medical Center, Durham, NC, USA
    Immunol Res 18:61-78. 1998
  10. ncbi request reprint HIV vaccine development at Duke University Medical Center
    B F Haynes
    Department of Medicine, The Duke Center for Aids Research, Duke University Medical Center, Durham, NC 27710, USA
    Immunol Res 22:263-9. 2000

Research Grants

  1. DESIGN OF NOVEL IMMUNOGENS AND ADJUVANTS FOR HIV VACCINE
    Barton Haynes; Fiscal Year: 2003
  2. NCRR FACSAria Cell Sorter
    Barton Haynes; Fiscal Year: 2004

Detail Information

Publications129 found, 100 shown here

  1. doi request reprint IGHV1-69 B cell chronic lymphocytic leukemia antibodies cross-react with HIV-1 and hepatitis C virus antigens as well as intestinal commensal bacteria
    Kwan Ki Hwang
    Duke Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina, United States of America
    PLoS ONE 9:e90725. 2014
    ..The B-CLL IGHV1-69 B cell usage of F54 allelic variants strongly suggests that IGHV1-69 B-CLL gp41 antibodies derive from a restricted B cell pool that also produces rare HIV-1 gp41 and influenza hemagglutinin stem antibodies. ..
  2. doi request reprint Progress in HIV-1 vaccine development
    Barton F Haynes
    Duke Human Vaccine Institute, Duke University Medical Center, Durham, NC 27710, USA
    Curr Opin HIV AIDS 8:326-32. 2013
    ..In this review, examples of recent progress in HIV-1 vaccine research are discussed...
  3. pmc B-cell-lineage immunogen design in vaccine development with HIV-1 as a case study
    Barton F Haynes
    Duke Human Vaccine Institute, Department of Medicine, Duke University School of Medicine, Durham, North Carolina, USA
    Nat Biotechnol 30:423-33. 2012
    ..This approach, which should be applicable to many infectious agents, holds promise for the construction of vaccines that can drive B cells along rare but desirable maturation pathways...
  4. pmc Immune-correlates analysis of an HIV-1 vaccine efficacy trial
    Barton F Haynes
    Duke University Human Vaccine Institute and the Center for HIV AIDS Vaccine Immunology, Duke University School of Medicine, Durham, NC 27710, USA
    N Engl J Med 366:1275-86. 2012
    ..In the RV144 trial, the estimated efficacy of a vaccine regimen against human immunodeficiency virus type 1 (HIV-1) was 31.2%. We performed a case-control analysis to identify antibody and cellular immune correlates of infection risk...
  5. ncbi request reprint The human thymus during aging
    B F Haynes
    Department of Medicine, Duke University Arthritis Center, Duke Human Vaccine Institute, Duke University School of Medicine, Durham, NC, USA
    Immunol Res 22:253-61. 2000
    ..This paper reviews work on human thymic aging at Duke University School of Medicine and discusses the relevance of this work to devising new strategies for T cell immune reconstitution in man...
  6. pmc Antibody polyspecificity and neutralization of HIV-1: a hypothesis
    Barton F Haynes
    Duke Human Vaccine Institute, Duke University School of Medicine, Durham NC 27710, USA
    Hum Antibodies 14:59-67. 2005
    ....
  7. ncbi request reprint Analysis of HIV-1 subtype B third variable region peptide motifs for induction of neutralizing antibodies against HIV-1 primary isolates
    Barton F Haynes
    Department of Medicine and Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA
    Virology 345:44-55. 2006
    ....
  8. ncbi request reprint The role of the thymus in immune reconstitution in aging, bone marrow transplantation, and HIV-1 infection
    B F Haynes
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Annu Rev Immunol 18:529-60. 2000
    ....
  9. ncbi request reprint The human thymus. A chimeric organ comprised of central and peripheral lymphoid components
    B F Haynes
    Department of Medicine, Duke University School of Medicine, Duke Medical Center, Durham, NC, USA
    Immunol Res 18:61-78. 1998
    ....
  10. ncbi request reprint HIV vaccine development at Duke University Medical Center
    B F Haynes
    Department of Medicine, The Duke Center for Aids Research, Duke University Medical Center, Durham, NC 27710, USA
    Immunol Res 22:263-9. 2000
    ..This brief review summarizes ongoing work at the Duke University School of Medicine on HIV vaccine development...
  11. ncbi request reprint Cardiolipin polyspecific autoreactivity in two broadly neutralizing HIV-1 antibodies
    Barton F Haynes
    Duke University School of Medicine, Durham, NC 27710, USA
    Science 308:1906-8. 2005
    ..These results may have important implications for generating effective neutralizing antibody responses by using HIV-1 vaccines...
  12. ncbi request reprint The human thymus. A chimeric organ comprised of central and peripheral lymphoid components
    B F Haynes
    Department of Medicine, Duke University School of Medicine, Duke Medical Center, Durham, NC, USA
    Immunol Res 18:175-92. 1998
    ....
  13. pmc Aiming to induce broadly reactive neutralizing antibody responses with HIV-1 vaccine candidates
    Barton F Haynes
    Box 3258, RP 1 Building, Building 107, Circuit Drive, Duke University Medical Center, Durham, NC 27710, USA
    Expert Rev Vaccines 5:347-63. 2006
    ....
  14. ncbi request reprint Aiming to induce broadly reactive neutralizing antibody responses with HIV-1 vaccine candidates
    Barton F Haynes
    Duke University Medical Center, Durham, NC 27710, USA
    Expert Rev Vaccines 5:579-95. 2006
    ....
  15. pmc HIV-1 hides an Achilles' heel in virion lipids
    Barton F Haynes
    Duke Human Vaccine Institute, Departments of Medicine, Pathology, and Immunology, Duke University School of Medicine, Durham, NC 27710, USA
    Immunity 28:10-2. 2008
    ..In this issue, Sun et al. (2008) model the interface between a neutralizing antibody and HIV-1 glycoprotein 41 (gp41) envelope peptide in lipids. This model could help overcome a roadblock in HIV-1 vaccine development...
  16. pmc Analysis of the adult thymus in reconstitution of T lymphocytes in HIV-1 infection
    B F Haynes
    Department of Medicine, Duke Center for AIDS Research, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Clin Invest 103:453-60. 1999
    ..Thymectomy before HIV-1 infection did not preclude either peripheral CD4(+) T-cell rises or clinical responses after antiretroviral therapy...
  17. pmc Critical issues in mucosal immunity for HIV-1 vaccine development
    Barton F Haynes
    Duke Human Vaccine Institute, Department of Medicine, Duke University School of Medicine, Durham, NC 27710, USA
    J Allergy Clin Immunol 122:3-9; quiz 10-1. 2008
    ..Thus basic and translational research to understand why current vaccines have failed and elucidation of new mechanisms of virus control at mucosal surfaces is essential for eventual successful development of a preventive HIV-1 vaccine...
  18. pmc Is developing an HIV-1 vaccine possible?
    Barton F Haynes
    Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina, USA
    Curr Opin HIV AIDS 5:362-7. 2010
    ..This review discusses select recent data that suggest that indeed it is possible to make a clinically useful preventive vaccine for HIV-1 and outlines some of the remaining obstacles that stand in the way of success...
  19. ncbi request reprint Comparison of thymocyte development and cytokine production in CD7-deficient, CD28-deficient and CD7/CD28 double-deficient mice
    C S Heinly
    Division of Rheumatology, Allergy and Clinical Immunology, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Int Immunol 13:157-66. 2001
    ..05). Thus, CD7 and CD28 deficiencies combined to produce abnormalities in the absolute number of B7-1/B7-2-expressing cells in the thymus, thymocyte IL-2 receptor expression and CD3-triggered cytokine production...
  20. ncbi request reprint Thymocyte LFA-1 and thymic epithelial cell ICAM-1 molecules mediate binding of activated human thymocytes to thymic epithelial cells
    K H Singer
    Department of Microbiology and Immunology, Duke University Medical Center, Durham, NC 27710
    J Immunol 144:2931-9. 1990
    ..We postulate that IFN-gamma produced intrathymically may regulate TE expression of ICAM-1 and therefore potentially may regulate TE cell binding to activated thymocytes beginning in the earliest stages of human thymic development...
  21. ncbi request reprint Transplantation of thymus tissue in complete DiGeorge syndrome
    M L Markert
    Department of Pediatrics, Duke Comprehensive Cancer Center, Duke University Medical Center, Durham, NC 27710, USA
    N Engl J Med 341:1180-9. 1999
    ..The DiGeorge syndrome is a congenital disorder that affects the heart, parathyroid glands, and thymus. In complete DiGeorge syndrome, patients have severely reduced T-cell function...
  22. ncbi request reprint Activated T lymphocytes regulate hyaluronan binding to monocyte CD44 via production of IL-2 and IFN-gamma
    M C Levesque
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    J Immunol 166:188-96. 2001
    ....
  23. ncbi request reprint Removal of fibroblasts from human epithelial cell cultures with use of a complement fixing monoclonal antibody reactive with human fibroblasts and monocytes/macrophages
    K H Singer
    Department of Medicine, Duke University, Durham, North Carolina 27710
    J Invest Dermatol 92:166-70. 1989
    ..We used the 1B10 antibody with complement to eliminate most 1B10 positive nonepithelial cells from thymic epithelial (TE) cell cultures, thereby allowing us to grow highly enriched populations of human TE cells...
  24. ncbi request reprint Effect of highly active antiretroviral therapy and thymic transplantation on immunoreconstitution in HIV infection
    M L Markert
    Department of Pediatrics, Duke University Medical Center, Durham, North Carolina 27710, USA
    AIDS Res Hum Retroviruses 16:403-13. 2000
    ..There was no clear difference in restoration of T cell function in the transplant recipients compared with the controls. Increases in TRECs after initiation of HAART may correlate with improved immune function...
  25. ncbi request reprint Leukemia inhibitory factor, oncostatin M, IL-6, and stem cell factor mRNA expression in human thymus increases with age and is associated with thymic atrophy
    G D Sempowski
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    J Immunol 164:2180-7. 2000
    ..p. to mice over 3 days induced thymic atrophy with loss of CD4+, CD8+ cortical thymocytes. Taken together, these data suggest a role for thymic cytokines in the process of thymic atrophy...
  26. ncbi request reprint Successful formation of a chimeric human thymus allograft following transplantation of cultured postnatal human thymus
    M L Markert
    Department of Pediatrics, Duke University Medical Center, Durham, NC 27710, USA
    J Immunol 158:998-1005. 1997
    ....
  27. ncbi request reprint Thymopoiesis in HIV-infected adults after highly active antiretroviral therapy
    M L Markert
    Department of Pediatrics, Duke University Medical Center, Durham, North Carolina 27710, USA
    AIDS Res Hum Retroviruses 17:1635-43. 2001
    ..Thymopoiesis in adult AIDS patients may contribute to immune reconstitution even after prolonged CD4+ T lymphopenia...
  28. ncbi request reprint Immunologic characterization of CD7-deficient mice
    D M Lee
    Department of Medicine, and Duke University Arthritis Center, Duke University Medical Center, Durham, NC 27710, USA
    J Immunol 160:5749-56. 1998
    ..These data suggest a role for CD7 in regulating intrathymic T cell development and in mediating CTL effecter function...
  29. pmc Stromal cell independent B cell development in vitro: generation and recovery of autoreactive clones
    T Matt Holl
    Department of Immunology, DUMC 3010, Duke University Medical Center, Durham, NC 27710, USA
    J Immunol Methods 354:53-67. 2010
    ..Furthermore, serum IgG autoantibody exceeded that present in autoimmune, C4(-/-) animals following the reconstitution of RAG1(-/-) mice with IgM+ CD cells derived from BL/6 mice...
  30. pmc Cytokines as adjuvants for the induction of anti-human immunodeficiency virus peptide immunoglobulin G (IgG) and IgA antibodies in serum and mucosal secretions after nasal immunization
    Curtis P Bradney
    Department of Medicine, Human Vaccine Institute, Center for AIDS Research, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Virol 76:517-24. 2002
    ..These results indicate that the proinflammatory cytokines IL-1alpha, IL-12, and IL-18 can replace CT as a mucosal adjuvant for antibody induction and are important candidates for use as mucosal adjuvants with HIV and other vaccines...
  31. pmc Induction of antibodies in guinea pigs and rhesus monkeys against the human immunodeficiency virus type 1 envelope: neutralization of nonpathogenic and pathogenic primary isolate simian/human immunodeficiency virus strains
    H X Liao
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Virol 74:254-63. 2000
    ..6. In contrast to natural infection with SHIV-89.6, in which few animals make anti-V3 antibodies, C4-V3 peptides frequently induced anti-V3 antibodies that neutralized primary isolate SHIV strains...
  32. ncbi request reprint AD2, a human molecule involved in the interaction of T cells with epidermal keratinocytes and thymic epithelial cells
    C S Bruggers
    Division of Hematology Oncology, Department of Pediatrics, Duke University Medical Center, Durham, NC 27710
    J Immunol 154:2012-22. 1995
    ..Taken together, these data suggest a role for the AD2 molecule in interactions of T cells with epithelial cells of skin and thymus...
  33. ncbi request reprint Functional association of CD7 with phosphatidylinositol 3-kinase: interaction via a YEDM motif
    D M Lee
    Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA
    Int Immunol 8:1195-203. 1996
    ..The interaction of CD7 with the Pi3-kinase signal transduction pathway provides a mechanism for the previously observed functional responses attributed to CD7-mediated T and NK cell activation...
  34. ncbi request reprint Cytokine requirements for induction of systemic and mucosal CTL after nasal immunization
    H F Staats
    Department of Medicine, Human Vaccine Institute, Duke University Medical Center, Durham, NC 27710, USA
    J Immunol 167:5386-94. 2001
    ..Thus, IL-1alpha, IL-12, IL-18, and GM-CSF are critical cytokines for the induction of systemic and mucosal CTL after nasal immunization. Moreover, these cytokines may serve as effective adjuvants for nasal vaccine delivery...
  35. ncbi request reprint Linkage of the CCR5 Delta 32 mutation with a functional polymorphism of CD45RA
    H X Liao
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    J Immunol 165:148-57. 2000
    ..w. CD45RA isoforms. Although the presence of the CCR5 Delta 32 mutation down-regulates HIV-1 infection of thymocytes, the functional CD45RA polymorphism does not alter the susceptibility of thymocytes to HIV-1 infection in vitro...
  36. ncbi request reprint Mapping of immunogenic regions of human T cell leukemia virus type I (HTLV-I) gp46 and gp21 envelope glycoproteins with env-encoded synthetic peptides and a monoclonal antibody to gp46
    T J Palker
    Department of Medicine, Duke University Medical Center, Durham, NC 27710
    J Immunol 142:971-8. 1989
    ..Thus, HTLV-I infection in man is associated with a major antibody response to a region of gp46 within amino acids 190 to 209 that is on the surface of virus-infected cells...
  37. ncbi request reprint Thymic function after hematopoietic stem-cell transplantation for the treatment of severe combined immunodeficiency
    D D Patel
    Department of Medicine, Human Vaccine Institute, Duke University Medical Center, Durham, NC 27710, USA
    N Engl J Med 342:1325-32. 2000
    ..The role of the thymus in this process is unknown...
  38. pmc Resistance of CD7-deficient mice to lipopolysaccharide-induced shock syndromes
    G D Sempowski
    Division of Rheumatology, Department of Medicine, Duke University Arthritis Center, Durham, North Carolina, 27710, USA
    J Exp Med 189:1011-6. 1999
    ..1(+)/ CD3(+) T cells, and is associated with resistance to LPS shock. These data suggest that CD7 is a key molecule in the inflammatory response leading to LPS-induced shock...
  39. ncbi request reprint A comparison of murine T-cell-depleted adult bone marrow and full-term fetal blood cells in hematopoietic engraftment and immune reconstitution
    Benny J Chen
    Department of Medicine and Immunology, Human Vaccine Institute, Duke University Medical Center, Durham, NC 27705, USA
    Blood 99:364-71. 2002
    ..These results suggest that full-term fetal blood can engraft allogeneic hosts across the major histocompatibility barrier with slower hematopoietic engraftment and impaired immune reconstitution...
  40. pmc Stable docking of neutralizing human immunodeficiency virus type 1 gp41 membrane-proximal external region monoclonal antibodies 2F5 and 4E10 is dependent on the membrane immersion depth of their epitope regions
    S Moses Dennison
    Human Vaccine Institute, Department of Medicine, Duke University School of Medicine, Durham, North Carolina 27710, USA
    J Virol 83:10211-23. 2009
    ..These data have important implications for the design and use of peptide-liposome conjugates as immunogens for the induction of MPER-neutralizing antibodies...
  41. ncbi request reprint The c-kit proto-oncogene receptor is expressed on a subset of human CD3-CD4-CD8- (triple-negative) thymocytes
    C M DeCastro
    Department of Medicine, Duke University Medical Center, Durham, NC 27710
    Exp Hematol 22:1025-33. 1994
    ..These data provide evidence that the c-kit receptor is present on human hematopoietic bone marrow and intrathymic T cell progenitor cells, and that it likely plays a role in early T cell lymphopoiesis...
  42. ncbi request reprint Developmental regulation of lymphocyte-specific protein 1 (LSP1) expression in thymus during human T-cell maturation
    T J Palker
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Hybridoma 17:497-507. 1998
    ..Thus, LSP1 is developmentally regulated during T-cell maturation within the human thymus and may play a functional role in the motility of DN and SP thymocytes...
  43. pmc Cross-subtype T-cell immune responses induced by a human immunodeficiency virus type 1 group m consensus env immunogen
    Eric A Weaver
    Duke Human Vaccine Institute, Duke University Medical Center, 112 RPIII, Research Drive, Box 3347, DUMC, Durham, NC 27710, USA
    J Virol 80:6745-56. 2006
    ....
  44. pmc Differential inhibition of human immunodeficiency virus type 1 in peripheral blood mononuclear cells and TZM-bl cells by endotoxin-mediated chemokine and gamma interferon production
    Anthony R Geonnotti
    Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA
    AIDS Res Hum Retroviruses 26:279-91. 2010
    ..The results also highlight the need to use endotoxin-free specimens to avoid artifacts when assessing HIV-1-specific neutralizing antibodies in PBMC-based assays...
  45. pmc Epitopes for broad and potent neutralizing antibody responses during chronic infection with human immunodeficiency virus type 1
    Avishek Nandi
    Division of Viral Pathogenesis, Department of Medicine, Beth Israel Deaconess Medical Center, E CLS 1011, 3 Blackfan Circle, Boston, MA 02215, USA
    Virology 396:339-48. 2010
    ..This study provides a more complete profile of epitope usage for broad and potent nAb responses during HIV-1 infection...
  46. pmc Recombinant Mycobacterium bovis bacillus Calmette-Guerin elicits human immunodeficiency virus type 1 envelope-specific T lymphocytes at mucosal sites
    Jae Sung Yu
    Human Vaccine Institute and Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Clin Vaccine Immunol 14:886-93. 2007
    ..However, rBCG could prime for a protein boost by HIV-1 envelope protein. Thus, rBCG can serve as a vector for induction of anti-HIV-1 consensus Env cellular responses at mucosal sites...
  47. ncbi request reprint The role of adhesion molecules in epithelial-T-cell interactions in thymus and skin
    K H Singer
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710
    J Invest Dermatol 94:85S-90S. 1990
    ..These antigen-independent adhesion molecules play an important role in the cell-cell interactions associated with T-cell differentiation and function...
  48. ncbi request reprint T-Cell recovery in adults and children following umbilical cord blood transplantation
    A K Klein
    Department of Medicine and the Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina, USA. span.org
    Biol Blood Marrow Transplant 7:454-66. 2001
    ....
  49. pmc Prolonged exposure of the HIV-1 gp41 membrane proximal region with L669S substitution
    Xiaoying Shen
    Department of Surgery, Duke Human Vaccine Institute, Duke University, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 107:5972-7. 2010
    ..These data suggest that a major contribution to the L669S mutant virus phenotype of enhanced susceptibility to MPER mAbs is prolonged exposure of the MPER neutralizing epitope during viral entry...
  50. pmc Generation of CD8+ T-cell responses by a recombinant nonpathogenic Mycobacterium smegmatis vaccine vector expressing human immunodeficiency virus type 1 Env
    Mark J Cayabyab
    Department of Medicine, Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Ave, Boston, MA 02130, USA
    J Virol 80:1645-52. 2006
    ..Importantly, preexisting immunity to Mycobacterium bovis BCG had only a marginal effect on the immunogenicity of recombinant M. smegmatis. This mycobacterium may therefore be a useful vaccine vector...
  51. pmc HIV-1 gp41 envelope IgA is frequently elicited after transmission but has an initial short response half-life
    N L Yates
    Duke Human Vaccine Institute, Duke University, Durham, North Carolina, USA
    Mucosal Immunol 6:692-703. 2013
    ..HIV-1 transmission frequently elicits mucosal HIV-1 envelope-specific IgA responses targeted to gp41 that have a short half-life...
  52. ncbi request reprint Changes in thymic function with age and during the treatment of HIV infection
    D C Douek
    Department of Medicine, The University of Texas Southwestern Medical Center, Dallas 75235, USA
    Nature 396:690-5. 1998
    ..In adults treated with HAART, there is a rapid and sustained increase in thymic output in most subjects. These results indicate that the adult thymus can contribute to immune reconstitution following HAART...
  53. ncbi request reprint Structure and function of the CD7 molecule
    G D Sempowski
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Crit Rev Immunol 19:331-48. 1999
    ..This article reviews recent work on the expression, structure, and function of CD7, and discusses roles the CD7 molecule might play in T and NK cell development and function...
  54. pmc In vivo gp41 antibodies targeting the 2F5 monoclonal antibody epitope mediate human immunodeficiency virus type 1 neutralization breadth
    Xiaoying Shen
    Duke Human Vaccine Institute, Duke University Medicine Center, Durham, NC 27710, USA
    J Virol 83:3617-25. 2009
    ..Our findings suggest that multiple events (i.e., genetic predisposition and HIV-1 immune dysregulation) may be required for induction of broadly reactive gp41 MPER antibodies in natural infection...
  55. pmc Leptin selectively augments thymopoiesis in leptin deficiency and lipopolysaccharide-induced thymic atrophy
    Ryan W Hick
    Human Vaccine Institute, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    J Immunol 177:169-76. 2006
    ..Thus, leptin has a selective thymostimulatory role in settings of leptin deficiency and endotoxin administration, and may be useful for protecting the thymus from damage and augmenting T cell reconstitution in these clinical states...
  56. pmc Generation of mucosal anti-human immunodeficiency virus type 1 T-cell responses by recombinant Mycobacterium smegmatis
    Jae Sung Yu
    Human Vaccine Institute and Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Clin Vaccine Immunol 13:1204-11. 2006
    ..However, immunization with recombinant M. smegmatis expressing HIV-1 Env was able to prime for an HIV-1 Env protein boost for the induction of anti-HIV-1 antibody responses...
  57. pmc Murine CD7 shares antigenic cross-reactivity with HSP-60
    Brandon A Howard
    Departments of Medicine, Duke University Medical Center, Duke Human Vaccine Institute, Durham, North Carolina, USA
    Hybridoma (Larchmt) 27:81-9. 2008
    ..These data demonstrated molecular mimicry of mCD7 with HSP-60, and leave open the question of surface expression of mCD7...
  58. ncbi request reprint Differentiation of human T cells
    S M Denning
    Department of Medicine, Duke University School of Medicine, Durham, North Carolina
    Clin Lab Med 8:1-14. 1988
    ..The impact of monoclonal antibody technology, the discovery of the T-cell receptor for antigen, and the discovery of mechanisms of thymocyte-thymic microenvironment interactions on the analysis of human T-cell development are emphasized...
  59. ncbi request reprint A pathogenetic role for TNF alpha in the syndrome of cachexia, arthritis, and autoimmunity resulting from tristetraprolin (TTP) deficiency
    G A Taylor
    Howard Hughes Medical Institute Laboratory, Duke University Medical Center, Durham, North Carolina 27710, USA
    Immunity 4:445-54. 1996
    ..TTP-deficient mice may serve as useful models of the autoimmune inflammatory state resulting from chronic effective TNF alpha excess...
  60. ncbi request reprint An inducible HIV type 1 gp41 HR-2 peptide-binding site on HIV type 1 envelope gp120
    S Munir Alam
    Department of Medicine, Duke Center for AIDS Research, Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27707, USA
    AIDS Res Hum Retroviruses 20:836-45. 2004
    ..This may prove to be an important consideration when designing an HIV vaccine that utilizes constrained HIV Env proteins...
  61. pmc Induction of plasma (TRAIL), TNFR-2, Fas ligand, and plasma microparticles after human immunodeficiency virus type 1 (HIV-1) transmission: implications for HIV-1 vaccine design
    Nancy Gasper-Smith
    Duke Human Vaccine Institute, Department of Medicine, Duke University School of Medicine, Durham, North Carolina 27710, USA
    J Virol 82:7700-10. 2008
    ....
  62. pmc Polyclonal B cell differentiation and loss of gastrointestinal tract germinal centers in the earliest stages of HIV-1 infection
    Marc C Levesque
    Department of Medicine, Duke University School of Medicine, Durham, North Carolina, United States of America
    PLoS Med 6:e1000107. 2009
    ..While the effect of HIV-1 on depletion of gut CD4(+) T cells in acute HIV-1 infection is well described, we studied blood and tissue B cells soon after infection to determine the effect of early HIV-1 on these cells...
  63. ncbi request reprint Increase in TCR gamma delta T lymphocytes in synovia from rheumatoid arthritis patients with active synovitis
    M R Jacobs
    Department of Medicine, Duke University Arthritis Center, Durham, North Carolina
    J Clin Immunol 12:130-8. 1992
    ..Thus, in cellular inflammatory infiltrates the presence of increased TCR gamma delta cells is not a component of noninfectious granulomatous inflammation but is found in approximately 40% of RA synovia with high levels of inflammation...
  64. ncbi request reprint Cytokines and adhesion molecules in the pathogenesis of vasculitis
    J S Sundy
    Division of Rheumatology, Allergy, and Clinical Immunology, Duke University Medical Center, Room 348 Sands Building, Box 3278 DUMC, Durham, NC 27710, USA
    Curr Rheumatol Rep 2:402-10. 2000
    ..Dysregulated adhesion molecule expression, uncontrolled inflammation, thrombosis, and vessel occlusion lead to the clinical manifestations of vasculitis...
  65. pmc Role of HIV membrane in neutralization by two broadly neutralizing antibodies
    S Munir Alam
    Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 106:20234-9. 2009
    ..These results bear directly on strategies for rational design of HIV-1 envelope immunogens...
  66. pmc Strategies for eliciting HIV-1 inhibitory antibodies
    Georgia D Tomaras
    Duke Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina, USA
    Curr Opin HIV AIDS 5:421-7. 2010
    ..We review examples of strategies for eliciting potentially protective HIV-1 inhibitory antibodies...
  67. pmc Initial B-cell responses to transmitted human immunodeficiency virus type 1: virion-binding immunoglobulin M (IgM) and IgG antibodies followed by plasma anti-gp41 antibodies with ineffective control of initial viremia
    Georgia D Tomaras
    Duke Human Vaccine Institute, Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    J Virol 82:12449-63. 2008
    ..These results demonstrate that the first IgM and IgG antibodies induced by transmitted HIV-1 are capable of binding virions but have little impact on acute-phase viremia at the timing and magnitude that they occur in natural infection...
  68. pmc Flow cytometry sorting of recombinant mycobacterial species yields bacterial clones with enhanced insert expression
    Jae Sung Yu
    Human Vaccine Institute and Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Clin Vaccine Immunol 18:43-9. 2011
    ..Thus, flow cytometry-based sorting can isolate recombinant mycobacteria enriched for higher insert expression...
  69. doi request reprint Crystal structure of a non-neutralizing antibody to the HIV-1 gp41 membrane-proximal external region
    Nathan I Nicely
    Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina, USA
    Nat Struct Mol Biol 17:1492-4. 2010
    ..We show that unlike 2F5, 13H11 binds to a well-defined helical MPER structure that is consistent with the structure of gp41 in a post-fusion six-helix bundle conformation...
  70. pmc B cell responses to HIV-1 infection and vaccination: pathways to preventing infection
    Barton F Haynes
    Duke Human Vaccine Institute and the Duke Center for AIDS Research, Department of Medicine, Duke University School of Medicine, Durham, NC 27710, USA
    Trends Mol Med 17:108-16. 2011
    ....
  71. pmc A group M consensus envelope glycoprotein induces antibodies that neutralize subsets of subtype B and C HIV-1 primary viruses
    Hua Xin Liao
    Duke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA
    Virology 353:268-82. 2006
    ..Our study also shows the feasibility of iterative improvements in Env immunogenicity by rational design of centralized genes...
  72. pmc Common human genetic variants and HIV-1 susceptibility: a genome-wide survey in a homogeneous African population
    Slave Petrovski
    Center for Human Genome Variation, School of Medicine, Duke University, Durham, North Carolina 27708, USA
    AIDS 25:513-8. 2011
    ..We investigated whether common genetic variants associate with HIV-1 susceptibility in Africans...
  73. pmc Utilization of immunoglobulin G Fc receptors by human immunodeficiency virus type 1: a specific role for antibodies against the membrane-proximal external region of gp41
    Lautaro G Perez
    Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Virol 83:7397-410. 2009
    ..The FcgammaR effects seen here suggests that the MPER of gp41 could have greater value for vaccines than previously recognized...
  74. pmc Anti-Ebola MAb 17A3 reacts with bovine and human alpha-2-macroglobulin proteins
    Jae Sung Yu
    Departments of Medicine, Human Vaccine Institute, Duke University Medical Center, Durham, NC 27710, USA
    J Virol Methods 168:248-50. 2010
    ..Thus, while MAbs 15H10 and 6D11 are indeed EBOV GP specific, MAb 17A3 is an alpha-2-macroglobulin MAb...
  75. pmc HIV-1 envelope induces memory B cell responses that correlate with plasma antibody levels after envelope gp120 protein vaccination or HIV-1 infection
    Mattia Bonsignori
    Duke Human Vaccine Institute, Duke University Medical Center, Durham, NC 27710, USA
    J Immunol 183:2708-17. 2009
    ..The inability to generate high titers of long-lived anti-envelope Abs is a major hurdle to overcome for the development of a successful HIV-1 vaccine...
  76. pmc Anti-phospholipid human monoclonal antibodies inhibit CCR5-tropic HIV-1 and induce beta-chemokines
    M Anthony Moody
    Duke Human Vaccine Institute, Duke University Medical Center, Durham, NC 27710, USA
    J Exp Med 207:763-76. 2010
    ..The release of these beta-chemokines explains both the specificity for R5 HIV-1 and the activity of these mAbs in PBMC cultures containing both primary lymphocytes and monocytes...
  77. pmc Phenotypic and functional profile of HIV-inhibitory CD8 T cells elicited by natural infection and heterologous prime/boost vaccination
    Stephanie A Freel
    Duke University Human Vaccine Institute, Departments of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Virol 84:4998-5006. 2010
    ..Our data define attributes of an antiviral CD8(+) T-cell response that may be optimized in the search for an efficacious HIV-1 vaccine...
  78. pmc In vitro and in vivo characterization of anthrax anti-protective antigen and anti-lethal factor monoclonal antibodies after passive transfer in a mouse lethal toxin challenge model to define correlates of immunity
    Herman F Staats
    Department of Pathology, Box 3712, DUMC, Durham, NC 27710, USA
    Infect Immun 75:5443-52. 2007
    ..Thus, this LeTx neutralization assay may be a more biologically relevant neutralization assay to predict the in vivo protective capacity of LeTx-neutralizing antibodies...
  79. pmc HIV-1-specific antibody responses during acute and chronic HIV-1 infection
    Georgia D Tomaras
    Duke Human Vaccine Institute, Duke University Medical Center, Durham, NC 27710, USA
    Curr Opin HIV AIDS 4:373-9. 2009
    ....
  80. pmc Cross-reactive monoclonal antibodies to multiple HIV-1 subtype and SIVcpz envelope glycoproteins
    Feng Gao
    Duke Human Vaccine Institute, Duke University Medical Center, Durham, NC 27710, USA
    Virology 394:91-8. 2009
    ..Nonetheless, such mAbs represent valuable reagents to study the biochemistry and structural biology of Env protein oligomers...
  81. pmc Autoreactivity in an HIV-1 broadly reactive neutralizing antibody variable region heavy chain induces immunologic tolerance
    Laurent Verkoczy
    Duke Human Vaccine Institute and Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 107:181-6. 2010
    ..These features are consistent with elimination of 2F5 HC autoreactivity by additional negative selection mechanism(s) in the periphery...
  82. pmc Functional, non-clonal IgMa-restricted B cell receptor interactions with the HIV-1 envelope gp41 membrane proximal external region
    Laurent Verkoczy
    Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina, United States of America
    PLoS ONE 4:e7215. 2009
    ..These data suggest that low avidity, non-paratopic interactions between the gp41 MPER and membrane Ig on naïve B cells may interfere with or divert bnAb responses...
  83. ncbi request reprint Centralized HIV-1 envelope immunogens and neutralizing antibodies
    Feng Gao
    Duke Human Vaccine Institute, Duke University Medical Center, Durham NC 27710, USA
    Curr HIV Res 5:572-7. 2007
    ....
  84. pmc Human T cell reconstitution in DiGeorge syndrome and HIV-1 infection
    Lori L Hudson
    Duke University Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    Semin Immunol 19:297-309. 2007
    ....
  85. pmc Human immunodeficiency virus type 1 gp41 antibodies that mask membrane proximal region epitopes: antibody binding kinetics, induction, and potential for regulation in acute infection
    S Munir Alam
    Human Vaccine Institute, Box 3258, Duke University Medical Center, MSRBII Bldg, Room 4042, Durham, NC 27710, USA
    J Virol 82:115-25. 2008
    ....
  86. pmc The role of antibody polyspecificity and lipid reactivity in binding of broadly neutralizing anti-HIV-1 envelope human monoclonal antibodies 2F5 and 4E10 to glycoprotein 41 membrane proximal envelope epitopes
    S Munir Alam
    Department of Medicine, Duke Human Vaccine Institute, Duke University School of Medicine, RP1 Circuit Drive, Durham, NC 27710, USA
    J Immunol 178:4424-35. 2007
    ..Taken together, these data demonstrate the similarity of 2F5 and 4E10 mAbs to known anti-cardiolipin Abs and support the model that mAb 2F5 and 4E10 binding to HIV-1 involves both viral lipid membrane and gp41 membrane proximal epitopes...
  87. ncbi request reprint Centralized immunogens as a vaccine strategy to overcome HIV-1 diversity
    Feng Gao
    The Duke Human Vaccine Institute, Department of Medicine, Duke University School of Medicine, Durham, NC 27710, USA
    Expert Rev Vaccines 3:S161-8. 2004
    ..It is biologically functional and preserves antigenicity similar to contemporary Env proteins. Most importantly, the group M consensus Env immunogen can elicit both T- and B-cell responses to wild-type HIV-1 isolates...
  88. ncbi request reprint Detection of Ebola virus envelope using monoclonal and polyclonal antibodies in ELISA, surface plasmon resonance and a quartz crystal microbalance immunosensor
    Jae Sung Yu
    Human Vaccine Institute, Duke University Medical Center, Durham, NC 27710, United States
    J Virol Methods 137:219-28. 2006
    ..Thus, polyclonal and monoclonal antibodies can be used in combination to identify and differentiate both human and non-human primate EBOV GPs...
  89. ncbi request reprint CD7 and CD28 are required for murine CD4+CD25+ regulatory T cell homeostasis and prevention of thyroiditis
    Gregory D Sempowski
    Department of Medicine and the Duke University Human Vaccine Institute, Duke University Medical Center, Box 3258, Research Park Building 1, Room 113, Research Drive, Durham, NC 27710, USA
    J Immunol 172:787-94. 2004
    ....
  90. ncbi request reprint Naïve T cells are maintained in the periphery during the first 3 months of acute HIV-1 infection: implications for analysis of thymus function
    Gregory D Sempowski
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Clin Immunol 25:462-72. 2005
    ....
  91. ncbi request reprint Expression of the CD7 ligand K-12 in human thymic epithelial cells: regulation by IFN-gamma
    Gordon K Lam
    Department of Medicine, Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina, USA
    J Clin Immunol 25:41-9. 2005
    ..These data suggest a role for thymic microenvironment-produced K12 in regulation of thymocyte signaling and cytokine release, particularly in the setting of thymus pathology where IFN-gamma is upregulated such as myasthenia gravis...
  92. pmc Immunogenicity of constrained monoclonal antibody A32-human immunodeficiency virus (HIV) Env gp120 complexes compared to that of recombinant HIV type 1 gp120 envelope glycoproteins
    Hua Xin Liao
    Duke Human Vaccine Institute and Department of Medicine, Duke University School of Medicine, Durham, NC 27710, USA
    J Virol 78:5270-8. 2004
    ....
  93. pmc Antigenicity and immunogenicity of a synthetic human immunodeficiency virus type 1 group m consensus envelope glycoprotein
    Feng Gao
    Department of Medicine, Duke University Medical Center, 112 Research Park III, Research Dr, Box 3347, Durham, NC 27710, USA
    J Virol 79:1154-63. 2005
    ..Thus, the computer-generated "consensus" env genes are capable of expressing envelope glycoproteins that retain the structural, functional, and immunogenic properties of wild-type HIV-1 envelopes...
  94. pmc Gender differences in human immunodeficiency virus type 1-specific CD8 responses in the reproductive tract and colon following nasal peptide priming and modified vaccinia virus Ankara boosting
    James W Peacock
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    J Virol 78:13163-72. 2004
    ..Understanding the cellular and molecular basis of gender-determined immune responses will be important for optimizing induction of anti-HIV-1 mucosal immune responses in both males and females...
  95. ncbi request reprint Thymus transplantation in complete DiGeorge syndrome: immunologic and safety evaluations in 12 patients
    M Louise Markert
    Department of Pediatrics, Duke University Medical Center, Durham, NC 27710, USA
    Blood 102:1121-30. 2003
    ..Thymic transplantation is efficacious, well tolerated, and should be considered as treatment for infants with complete DiGeorge syndrome...
  96. ncbi request reprint Leukemia inhibitory factor is a mediator of Escherichia coli lipopolysaccharide-induced acute thymic atrophy
    Gregory D Sempowski
    Department of Medicine, Human Vaccine Institute, Duke University Medical Center, Durham, USA
    Eur J Immunol 32:3066-70. 2002
    ..These data demonstrate systemic and intrathymic pathways of E. coli LPS-induced acute thymic atrophy mediated by LIF and corticosteroids...
  97. ncbi request reprint Immune reconstitution in patients with HIV infection
    Gregory D Sempowski
    Department of Medicine and the Center For AIDS Research and Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    Annu Rev Med 53:269-84. 2002
    ..New strategies to overcome the thymic atrophy of aging are needed to improve thymic function in the majority of AIDS patients...
  98. ncbi request reprint T cell receptor excision circle assessment of thymopoiesis in aging mice
    Gregory D Sempowski
    Department of Medicine, Center for AIDS Research, Human Vaccine Institute, Duke University Medical Center, Box 3258 Research Dr, Durham, NC 27710, USA
    Mol Immunol 38:841-8. 2002
    ..Thus, thymus output continues throughout murine adult life, and the thymic atrophy of aging in mice is not reversed by administration of IL-7...
  99. ncbi request reprint Increased immunogenicity of HIV envelope subunit complexed with alpha2-macroglobulin when combined with monophosphoryl lipid A and GM-CSF
    Hua Xin Liao
    Department of Medicine, Duke Human Vaccine Institute, Duke University Medical Center, Box 3258, Durham, NC 27710, USA
    Vaccine 20:2396-403. 2002
    ....
  100. ncbi request reprint Human T-cell lymphotropic virus I and adult T-cell leukemia: report of a cluster in North Carolina
    J B Weinberg
    Department of Medicine, Veterans Administration Medical Center, Durham, North Carolina 27705
    Am J Med 85:51-8. 1988
    ..Following the presentation of a patient who was infected with the virus, we undertook a study of his family members and sexual contacts to see if a cluster of infected persons could be identified...
  101. ncbi request reprint Engraftment of human synovium into severe combined immune deficient mice. Migration of human peripheral blood T cells to engrafted human synovium and to mouse lymph nodes
    K E Rendt
    Department of Medicine, Duke University Medical Center, Durham, NC
    J Immunol 151:7324-36. 1993
    ..Our data demonstrate that normal and inflammatory human synovial tissues will grow in SCID mice and serve as recipients for autologous and allogenic peripheral blood human T cells injected i.p. into engrafted mice...

Research Grants4

  1. DESIGN OF NOVEL IMMUNOGENS AND ADJUVANTS FOR HIV VACCINE
    Barton Haynes; Fiscal Year: 2003
    ..abstract_text> ..
  2. NCRR FACSAria Cell Sorter
    Barton Haynes; Fiscal Year: 2004
    ..Advisory committees, institutional support, financial support for continued maintenance, and management plans are in place to insure that the instrument will be fully and appropriately utilized. ..