F Guilak

Summary

Affiliation: Duke University Medical Center
Country: USA

Publications

  1. ncbi Monolayer cell expansion conditions affect the chondrogenic potential of adipose-derived stem cells
    Bradley T Estes
    Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA
    Biotechnol Bioeng 99:986-95. 2008
  2. ncbi Increased susceptibility of Trpv4-deficient mice to obesity and obesity-induced osteoarthritis with very high-fat diet
    Christopher J O'Conor
    Department of Orthopaedic Surgery, Duke University Medical Center, 375 MSRB, Box 3093, Durham, NC 27710, USA
    Ann Rheum Dis 72:300-4. 2013
  3. ncbi Interleukin-1, tumor necrosis factor-alpha, and transforming growth factor-beta 1 and integrative meniscal repair: influences on meniscal cell proliferation and migration
    Katherine M Riera
    Department of Orthopaedic Surgery, Duke University Medical Center, DUMC Box 3093, Durham, NC 27710, USA
    Arthritis Res Ther 13:R187. 2011
  4. ncbi Biomechanical factors in osteoarthritis
    Farshid Guilak
    Departments of Orthopaedic Surgery and Biomedical Engineering, Duke University Medical Center, Durham, NC 27710, USA
    Best Pract Res Clin Rheumatol 25:815-23. 2011
  5. ncbi Clinical and preclinical translation of cell-based therapies using adipose tissue-derived cells
    Jeffrey M Gimble
    Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, 6400 Perkins Road, Baton Rouge, LA 70808, USA
    Stem Cell Res Ther 1:19. 2010
  6. ncbi Diet-induced obesity differentially regulates behavioral, biomechanical, and molecular risk factors for osteoarthritis in mice
    Timothy M Griffin
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    Arthritis Res Ther 12:R130. 2010
  7. ncbi Zonal uniformity in mechanical properties of the chondrocyte pericellular matrix: micropipette aspiration of canine chondrons isolated by cartilage homogenization
    Farshid Guilak
    Orthopaedic Research Laboratories, Departments of Surgery, Duke University Medical Center, 375 MSR Bldg, Box 3093, Durham, NC 27710, USA
    Ann Biomed Eng 33:1312-8. 2005
  8. ncbi Transient receptor potential vanilloid 4: The sixth sense of the musculoskeletal system?
    Farshid Guilak
    Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA
    Ann N Y Acad Sci 1192:404-9. 2010
  9. ncbi Clonal analysis of the differentiation potential of human adipose-derived adult stem cells
    Farshid Guilak
    Orthopaedic Research Laboratories, Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Cell Physiol 206:229-37. 2006
  10. ncbi Functional assessment of engineered tissues and elements of tissue design: breakout session summary
    Farshid Guilak
    Department of Surgery, Duke University Medical Center, Room 374 MSRB, Durham, NC 27710, USA
    Ann N Y Acad Sci 961:207-9. 2002

Research Grants

  1. Intrinsic Repair of the Knee Meniscus
    Farshid Guilak; Fiscal Year: 2009
  2. VISCOELASTIC PROPERTIES OF NORMAL AND OA CHONDRONS
    Farshid Guilak; Fiscal Year: 2009
  3. Mechanical and Osmotic Signaling in Chondrocytes
    Farshid Guilak; Fiscal Year: 2009
  4. Intrinsic Repair of the Knee Meniscus
    Farshid Guilak; Fiscal Year: 2009
  5. VISCOELASTIC PROPERTIES OF NORMAL AND OA CHONDRONS
    Farshid Guilak; Fiscal Year: 2010
  6. Intrinsic Repair of the Knee Meniscus
    Farshid Guilak; Fiscal Year: 2010
  7. Mechanical and Osmotic Signaling in Chondrocytes
    Farshid Guilak; Fiscal Year: 2010
  8. Training in Biomolecular and Tissue Engineering
    Farshid Guilak; Fiscal Year: 2007
  9. VISCOELASTIC PROPERTIES OF NORMAL AND OA CHONDRONS
    Farshid Guilak; Fiscal Year: 2007
  10. VISCOELASTIC PROPERTIES OF NORMAL AND OA CHONDRONS
    Farshid Guilak; Fiscal Year: 2002

Detail Information

Publications108 found, 100 shown here

  1. ncbi Monolayer cell expansion conditions affect the chondrogenic potential of adipose-derived stem cells
    Bradley T Estes
    Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA
    Biotechnol Bioeng 99:986-95. 2008
    ....
  2. ncbi Increased susceptibility of Trpv4-deficient mice to obesity and obesity-induced osteoarthritis with very high-fat diet
    Christopher J O'Conor
    Department of Orthopaedic Surgery, Duke University Medical Center, 375 MSRB, Box 3093, Durham, NC 27710, USA
    Ann Rheum Dis 72:300-4. 2013
    ....
  3. ncbi Interleukin-1, tumor necrosis factor-alpha, and transforming growth factor-beta 1 and integrative meniscal repair: influences on meniscal cell proliferation and migration
    Katherine M Riera
    Department of Orthopaedic Surgery, Duke University Medical Center, DUMC Box 3093, Durham, NC 27710, USA
    Arthritis Res Ther 13:R187. 2011
    ..The goal of this study was to examine the hypothesis that IL-1 and TNF-α suppress, while TGF-β1 enhances, cellular proliferation and migration in cell and tissue models of meniscal repair...
  4. ncbi Biomechanical factors in osteoarthritis
    Farshid Guilak
    Departments of Orthopaedic Surgery and Biomedical Engineering, Duke University Medical Center, Durham, NC 27710, USA
    Best Pract Res Clin Rheumatol 25:815-23. 2011
    ..An improved understanding of the biophysical and molecular pathways involved in chondrocyte mechanotransduction can provide insight into the development of novel therapeutic approaches for osteoarthritis...
  5. ncbi Clinical and preclinical translation of cell-based therapies using adipose tissue-derived cells
    Jeffrey M Gimble
    Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, 6400 Perkins Road, Baton Rouge, LA 70808, USA
    Stem Cell Res Ther 1:19. 2010
    ..Future directions and challenges facing the field are discussed and evaluated...
  6. ncbi Diet-induced obesity differentially regulates behavioral, biomechanical, and molecular risk factors for osteoarthritis in mice
    Timothy M Griffin
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    Arthritis Res Ther 12:R130. 2010
    ..In this study, we examined biomechanical, neurobehavioral, inflammatory, and osteoarthritic changes in C57BL/6J mice fed a high-fat diet...
  7. ncbi Zonal uniformity in mechanical properties of the chondrocyte pericellular matrix: micropipette aspiration of canine chondrons isolated by cartilage homogenization
    Farshid Guilak
    Orthopaedic Research Laboratories, Departments of Surgery, Duke University Medical Center, 375 MSR Bldg, Box 3093, Durham, NC 27710, USA
    Ann Biomed Eng 33:1312-8. 2005
    ....
  8. ncbi Transient receptor potential vanilloid 4: The sixth sense of the musculoskeletal system?
    Farshid Guilak
    Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA
    Ann N Y Acad Sci 1192:404-9. 2010
    ..These studies provide mounting evidence for a regulatory role for the sensory channel TRPV4 in control of musculoskeletal tissues...
  9. ncbi Clonal analysis of the differentiation potential of human adipose-derived adult stem cells
    Farshid Guilak
    Orthopaedic Research Laboratories, Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Cell Physiol 206:229-37. 2006
    ..However, it is important to exercise caution in interpreting these results until they are validated using functional in vivo assays...
  10. ncbi Functional assessment of engineered tissues and elements of tissue design: breakout session summary
    Farshid Guilak
    Department of Surgery, Duke University Medical Center, Room 374 MSRB, Durham, NC 27710, USA
    Ann N Y Acad Sci 961:207-9. 2002
  11. ncbi Functional tissue engineering: the role of biomechanics in reparative medicine
    Farshid Guilak
    Department of Surgery, Duke University Medical Center, Room 374 MSRB, Durham, NC 27710, USA
    Ann N Y Acad Sci 961:193-5. 2002
  12. ncbi 2010 Nicolas Andry Award: Multipotent adult stem cells from adipose tissue for musculoskeletal tissue engineering
    Farshid Guilak
    Departments of Surgery and Biomedical Engineering, Duke University Medical Center, Durham, NC 27710, USA
    Clin Orthop Relat Res 468:2530-40. 2010
    ....
  13. ncbi Control of stem cell fate by physical interactions with the extracellular matrix
    Farshid Guilak
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    Cell Stem Cell 5:17-26. 2009
    ..Here, we review some of the physical processes by which cues from the ECM can influence stem cell fate, with particular relevance to the use of stem cells in tissue engineering and regenerative medicine...
  14. ncbi Homing in on a biological joint replacement
    Farshid Guilak
    Departments of Orthopaedic Surgery and Biomedical Engineering, Duke, University Medical Center, Durham, NC 27710, USA
    Stem Cell Res Ther 1:40. 2010
    ..These findings provide a glimpse into the possibility that tissue engineering may in fact provide the means for regeneration of an entire joint surface, beyond a simple focal defect in the articular cartilage...
  15. ncbi Adipose-derived adult stem cells for cartilage tissue engineering
    Farshid Guilak
    Departments of Surgery and Biomedical Engineering, Duke University Medical Center, Durham, NC 27710, USA
    Biorheology 41:389-99. 2004
    ..Overall, ADAS cells show significant promise for the development of functional tissue replacements for various tissues of the musculoskeletal system...
  16. ncbi The deformation behavior and mechanical properties of chondrocytes in articular cartilage
    F Guilak
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    Osteoarthritis Cartilage 7:59-70. 1999
    ..A fundamental step in determining the role of various biophysical factors in this process is to characterize the local mechanical environment of the chondrocyte under physiological loading...
  17. ncbi Mechanically induced calcium waves in articular chondrocytes are inhibited by gadolinium and amiloride
    F Guilak
    Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA
    J Orthop Res 17:421-9. 1999
    ....
  18. ncbi The mechanical environment of the chondrocyte: a biphasic finite element model of cell-matrix interactions in articular cartilage
    F Guilak
    Orthopaedic Research Laboratories, Departments of Surgery, Biomedical Engineering, and Mechanical Engineering and Materials Science, Duke University Medical Center, P O Box 3093, 375 MSRB, 27710, Durham, NC, USA
    J Biomech 33:1663-73. 2000
    ..Knowledge of the local stress and strain fields in the extracellular matrix is an important step in the interpretation of studies of mechanical signal transduction in cartilage explant culture models...
  19. ncbi The pericellular matrix as a transducer of biomechanical and biochemical signals in articular cartilage
    Farshid Guilak
    Orthopaedic Research Laboratories, Department of Surgery, Division of Orthopaedic Surgery, 375 MSRB, Box 3093, Duke University Medical Center, Durham, NC 27710, USA
    Ann N Y Acad Sci 1068:498-512. 2006
    ..An improved understanding of the structure and function of the PCM may provide new insights into the mechanisms that regulate chondrocyte physiology in health and disease...
  20. ncbi The effects of osmotic stress on the viscoelastic and physical properties of articular chondrocytes
    Farshid Guilak
    Orthopaedic Research Laboratories, Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710 USA
    Biophys J 82:720-7. 2002
    ..These findings indicate that the physicochemical environment has a strong influence on the viscoelastic and physical properties of the chondrocyte, potentially through alterations in the actin cytoskeleton...
  21. ncbi The effects of static and intermittent compression on nitric oxide production in articular cartilage explants
    B Fermor
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    J Orthop Res 19:729-37. 2001
    ..These findings indicate that NO production by chondrocytes is influenced by mechanical compression in vitro and suggest that biomechanical factors may in part regulate NO production in vivo...
  22. ncbi The effect of dynamic mechanical compression on nitric oxide production in the meniscus
    C Fink
    Department of Surgery, Division of Orthopaedic Surgery, Duke University Medical Center, Durham, NC 27710, USA
    Osteoarthritis Cartilage 9:481-7. 2001
    ....
  23. ncbi Mechanical stress and nitric oxide influence leukotriene production in cartilage
    B Fermor
    Department of Surgery, Division of Orthopedic Surgery, Duke University Medical Center, Durham, NC 27710, USA
    Biochem Biophys Res Commun 285:806-10. 2001
    ..These findings provide a direct link between mechanical stress and inflammation in cartilage and may have implications in the pathogenesis and treatment of arthritis...
  24. ncbi Interleukin-1 inhibits osmotically induced calcium signaling and volume regulation in articular chondrocytes
    S Pritchard
    Departments of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    Osteoarthritis Cartilage 16:1466-73. 2008
    ..The goal of this study was to examine the hypothesis that interleukin-1 (IL-1), a pro-inflammatory cytokine associated with osteoarthritis, influences osmotically induced Ca(2+) signaling...
  25. ncbi Interleukin-1, tumor necrosis factor alpha, and interleukin-17 synergistically up-regulate nitric oxide and prostaglandin E2 production in explants of human osteoarthritic knee menisci
    A Legrand
    Duke University Medical Center, Durham, North Carolina 27710, USA
    Arthritis Rheum 44:2078-83. 2001
    ..NO and PGE2 exhibited reciprocal regulatory effects on one another, suggesting that pharmaceutical agents designed to inhibit NOS2 or COX-2 production may in fact be influencing both pathways...
  26. ncbi Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate, and gelatin scaffolds
    Hani A Awad
    Department of Surgery, Division of Orthopaedic Surgery, Duke University Medical Center, Durham, NC 27710, USA
    Biomaterials 25:3211-22. 2004
    ....
  27. ncbi Hyper-osmotic stress induces volume change and calcium transients in chondrocytes by transmembrane, phospholipid, and G-protein pathways
    G R Erickson
    Orthopaedic Research Laboratories, Department of Surgery, Duke University Medical Center, 375 MSRB, Box 3093, Durham, NC 27710, USA
    J Biomech 34:1527-35. 2001
    ..These findings indicate that osmotic stress causes significant volume change in chondrocytes and may activate an intracellular second messenger signal by inducing transient increases in [Ca(2+)](i)...
  28. ncbi Hypo-osmotic stress induces calcium-dependent actin reorganization in articular chondrocytes
    G R Erickson
    Orthopaedic Research Laboratories, Department of Surgery, Duke University Medical Center, 27710, Durham, NC, USA
    Osteoarthritis Cartilage 11:187-97. 2003
    ..CONCLUSION: These results indicate that hypo-osmotic stress induces a gelsolin-mediated reorganization of actin through a transient increase in [Ca(2+)](i)...
  29. ncbi Dynamic loading enhances integrative meniscal repair in the presence of interleukin-1
    A L McNulty
    Department of Surgery, Duke University Medical Center, Durham, NC, USA
    Osteoarthritis Cartilage 18:830-8. 2010
    ..The purpose of this study was to determine the effects of mechanical compression on meniscal repair under normal and inflammatory conditions...
  30. ncbi Acute joint pathology and synovial inflammation is associated with increased intra-articular fracture severity in the mouse knee
    J S Lewis
    Department of Orthopaedic Surgery, Duke University Medical Center, Durham, NC 27710, USA
    Osteoarthritis Cartilage 19:864-73. 2011
    ..The objective of this investigation was to examine the effect of fracture severity on acute joint pathology in a novel murine model of intra-articular fracture...
  31. ncbi Viscoelastic properties of intervertebral disc cells. Identification of two biomechanically distinct cell populations
    F Guilak
    Department of Surgery, Duke University Medical Center, Durham, North Carolina, USA
    Spine (Phila Pa 1976) 24:2475-83. 1999
    ....
  32. ncbi Chondrocytic differentiation of human adipose-derived adult stem cells in elastin-like polypeptide
    Helawe Betre
    Department of Biomedical Engineering, 136 Hudson Hall, Box 90821, Duke University, Durham, NC 27708, USA
    Biomaterials 27:91-9. 2006
    ..The study suggests that ELP can promote chondrogenesis for hADAS cells in the absence of exogenous TGF-beta1 and dexamethasone, especially under low oxygen tension conditions...
  33. ncbi Isolation of adipose-derived stem cells and their induction to a chondrogenic phenotype
    Bradley T Estes
    Departments of Surgery and Biomedical Engineering, Duke University Medical Center, Durham, North Carolina, USA
    Nat Protoc 5:1294-311. 2010
    ..Approximately 10-12 weeks are needed for the entire process of ASC isolation, including the characterization of chondrocyte-like cells, which is also described...
  34. ncbi Zonal variations in the three-dimensional morphology of the chondron measured in situ using confocal microscopy
    I Youn
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    Osteoarthritis Cartilage 14:889-97. 2006
    ..Quantitative measurements of morphology of the chondron and its variation with site, disease, or aging may provide new insights into the influence of this structure on physiology and the pathology of articular cartilage...
  35. ncbi Functional properties of cell-seeded three-dimensionally woven poly(epsilon-caprolactone) scaffolds for cartilage tissue engineering
    Franklin T Moutos
    Department of Surgery, Duke University Medical Center, Durham, NC, USA
    Tissue Eng Part A 16:1291-301. 2010
    ....
  36. ncbi Reactive nitrogen and oxygen species in interleukin-1-mediated DNA damage associated with osteoarthritis
    C M Davies
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    Osteoarthritis Cartilage 16:624-30. 2008
    ..The aims of this study were to determine if OA cartilage shows evidence of DNA damage, and if IL-1 could induce DNA damage in non-OA cartilage by increasing NO or superoxide...
  37. ncbi Viscoelastic properties of zonal articular chondrocytes measured by atomic force microscopy
    E M Darling
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    Osteoarthritis Cartilage 14:571-9. 2006
    ..Given the versatility and dynamic testing capabilities of AFM, the ability to conduct stress-relaxation measurements using this technique may provide further insight into the viscoelastic properties of isolated cells...
  38. ncbi The effect of hip stem material modulus on surface strain in human femora
    T P Vail
    Division of Orthopaedic Surgery, Duke University Medical Center, Durham, NC 27710, USA
    J Biomech 31:619-28. 1998
    ..Although there was statistically greater surface shear strain as the material modulus decreased, the PMMA stem did not substantially increase bone loading...
  39. ncbi Alterations in the mechanical properties of the human chondrocyte pericellular matrix with osteoarthritis
    Leonidas G Alexopoulos
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    J Biomech Eng 125:323-33. 2003
    ..Furthermore, the significant loss of PCM stiffness that was observed in osteoarthritic cartilage may affect the magnitude and distribution of biomechanical signals perceived by the chondrocytes...
  40. ncbi The influence of mechanical compression on the induction of osteoarthritis-related biomarkers in articular cartilage explants
    J L Piscoya
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    Osteoarthritis Cartilage 13:1092-9. 2005
    ..These findings indicate that mechanical factors, independent of exogenous cytokines or other stimulatory factors, can influence the production and release of OA-related biomarkers from articular cartilage...
  41. ncbi Novel synovial fluid recovery method allows for quantification of a marker of arthritis in mice
    D R Seifer
    Department of Medicine, Division of Rheumatology, Duke University Medical Center, Durham, NC 27710, United States
    Osteoarthritis Cartilage 16:1532-8. 2008
    ....
  42. ncbi Interleukin-1 and tumor necrosis factor alpha inhibit repair of the porcine meniscus in vitro
    A Hennerbichler
    Department of Surgery, Division of Orthopaedic Surgery, Duke University Medical Center, Durham, NC 27710, USA
    Osteoarthritis Cartilage 15:1053-60. 2007
    ..The goal of this study was to investigate the capacity of meniscus tissue to repair a simulated defect in vitro and to examine the effect of pro-inflammatory cytokines on this process...
  43. ncbi Inhibition of integrative repair of the meniscus following acute exposure to interleukin-1 in vitro
    Rebecca E Wilusz
    Department of Surgery, Duke University Medical Center, Box 3093, Durham, North Carolina 27710, USA
    J Orthop Res 26:504-12. 2008
    ..The suppression of IL-1 or MMP expression and/or activity warrant investigation as potential strategies for promoting meniscal repair...
  44. ncbi Influence of hypoxia and reoxygenation on cytokine-induced production of proinflammatory mediators in articular cartilage
    Julie Cernanec
    Duke University Medical Center, Durham, North Carolina 27710, USA
    Arthritis Rheum 46:968-75. 2002
    ..Hypoxia and reoxygenation can affect cytokine-induced proinflammatory mediator production, suggesting that oxygen tension may influence inflammation associated with cartilage injury and disease...
  45. ncbi The role of the cytoskeleton in the viscoelastic properties of human articular chondrocytes
    Wendy R Trickey
    Departments of Surgery and Biomedical Engineering, Orthopaedic Research Laboratories, Duke University Medical Center, 375 Medical Sciences Research Bldg, P.O. Box 3093, Durham, NC 27710, USA
    J Orthop Res 22:131-9. 2004
    ....
  46. ncbi Chondrogenesis of adult stem cells from adipose tissue and bone marrow: induction by growth factors and cartilage-derived matrix
    Brian O Diekman
    Departments of Surgery and Biomedical Engineering, Duke University Medical Center, Durham, North Carolina 27710, USA
    Tissue Eng Part A 16:523-33. 2010
    ..We hypothesized that these cells show distinct responses to different chondrogenic culture conditions and extracellular matrices, illustrating important differences between cell types...
  47. ncbi Extended passaging, but not aldehyde dehydrogenase activity, increases the chondrogenic potential of human adipose-derived adult stem cells
    Bradley T Estes
    Departments of Surgery and Biomedical Engineering, Duke University Medical Center, Durham, NC 27710, USA
    J Cell Physiol 209:987-95. 2006
    ..These findings suggest that the chondrogenic potential of ADAS cells increases with passage number, although ALDH may not be a suitable marker for chondrogenesis...
  48. ncbi Influence of oxygen tension on interleukin 1-induced peroxynitrite formation and matrix turnover in articular cartilage
    Julie M Cernanec
    Department of Surgery, Division of Orthopaedic Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Rheumatol 34:401-7. 2007
    ..Cartilage is avascular and functions at reduced oxygen tension. We investigated whether oxygen tension influences the effects of interleukin 1 (IL-1) on peroxynitrite formation and cartilage matrix metabolism...
  49. ncbi Osmotic loading to determine the intrinsic material properties of guinea pig knee cartilage
    Charlene M Flahiff
    Department of Biomedical Engineering, Duke University, Box 90281, 136 Hudson Hall, Durham, NC 22708-0281, USA
    J Biomech 35:1285-90. 2002
    ....
  50. ncbi Determination of the Poisson's ratio of the cell: recovery properties of chondrocytes after release from complete micropipette aspiration
    Wendy R Trickey
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    J Biomech 39:78-87. 2006
    ..In contrast to previous assumptions, these findings suggest that chondrocytes are compressible, consistent with previous studies showing cell volume changes with compression of the extracellular matrix...
  51. ncbi The role of F-actin in hypo-osmotically induced cell volume change and calcium signaling in anulus fibrosus cells
    Scott Pritchard
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    Ann Biomed Eng 32:103-11. 2004
    ..In conclusion, our findings suggest that the F-actin network plays an important role in the response of AF cells to osmotic stress...
  52. ncbi Nonlinear osmotic properties of the cell nucleus
    John D Finan
    Department of Surgery, Duke University Medical Center, 375 MSRB, Box 3093, Durham, NC 27710, USA
    Ann Biomed Eng 37:477-91. 2009
    ..As nuclear morphology has been associated previously with cell phenotype, these findings may provide new insight into the role of mechanical and osmotic signals in regulating cell physiology...
  53. ncbi Functional characterization of TRPV4 as an osmotically sensitive ion channel in porcine articular chondrocytes
    Mimi N Phan
    Duke University Medical Center, Durham, North Carolina 27710, USA
    Arthritis Rheum 60:3028-37. 2009
    ..This study was undertaken to demonstrate the presence and functionality of TRPV4 in chondrocytes...
  54. ncbi Chondrogenic differentiation of adipose-derived adult stem cells by a porous scaffold derived from native articular cartilage extracellular matrix
    Nai Chen Cheng
    Departments of Surgery and Biomedical Engineering, Duke University Medical Center, Durham, North Carolina 27710, USA
    Tissue Eng Part A 15:231-41. 2009
    ..These findings support the potential for a processed cartilage ECM as a biomaterial scaffold for cartilage tissue engineering. Additional in vivo evaluation is necessary to fully recognize the clinical implication of these observations...
  55. ncbi Transfer of macroscale tissue strain to microscale cell regions in the deformed meniscus
    Maureen L Upton
    Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
    Biophys J 95:2116-24. 2008
    ....
  56. ncbi Nitric oxide synthase and cyclooxygenase interactions in cartilage and meniscus: relationships to joint physiology, arthritis, and tissue repair
    J Brice Weinberg
    Department of Medicine, Division of Hematology Oncology, VA and Duke University Medical Centers, Durham, North Carolina 27705, USA
    Subcell Biochem 42:31-62. 2007
    ....
  57. ncbi Microscale diffusion properties of the cartilage pericellular matrix measured using 3D scanning microphotolysis
    Holly A Leddy
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    J Biomech Eng 130:061002. 2008
    ..Our results support the hypothesis that diffusivity within the PCM of healthy articular cartilage is lower than that within the ECM, presumably due to differences in proteoglycan content...
  58. ncbi Sex differences in biomechanics associated with knee osteoarthritis
    Ershela L Sims
    Department of Biological Anthropology and Anatomy, Duke University Medical Center, 202 Biological Sciences Building, Box 90383, Durham, NC 27708, USA
    J Women Aging 21:159-70. 2009
    ..Within female subjects, variations in gait mechanics were primarily explained by weight, BMI, pain, and disability. In males, variations in gait mechanics were primarily explained by age and disability...
  59. ncbi Three-dimensional morphology of the pericellular matrix of intervertebral disc cells in the rat
    Li Cao
    Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
    J Anat 211:444-52. 2007
    ..These studies provide new information on the organization and shape of intervertebral disc cells and their surrounding pericellular matrix, which may provide new insights into the mechanisms that regulate cell-matrix interactions...
  60. ncbi Racial differences in gait mechanics associated with knee osteoarthritis
    Ershela L Sims
    Department of Surgery, Duke University, Durham, NC, USA
    Aging Clin Exp Res 21:463-9. 2009
    ....
  61. ncbi Urea as a passive transport marker for arthritis biomarker studies
    Virginia B Kraus
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Arthritis Rheum 46:420-7. 2002
    ....
  62. ncbi Label-free, high-throughput measurements of dynamic changes in cell nuclei using angle-resolved low coherence interferometry
    Kevin J Chalut
    Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
    Biophys J 94:4948-56. 2008
    ..Given the high-throughput nature of the measurements, this deviation may be acceptable for many biological applications that seek to establish connections between morphology and function...
  63. ncbi Loss of cartilage structure, stiffness, and frictional properties in mice lacking PRG4
    Jeffrey M Coles
    Duke University, Durham, North Carolina 27708, USA
    Arthritis Rheum 62:1666-74. 2010
    ..To assess the role of the glycoprotein PRG4 in joint lubrication and chondroprotection by measuring friction, stiffness, surface topography, and subsurface histology of the hip joints of Prg4(-/-) and wild-type (WT) mice...
  64. ncbi Joint degeneration following closed intraarticular fracture in the mouse knee: a model of posttraumatic arthritis
    Bridgette D Furman
    Division of Orthopaedic Surgery, Department of Surgery, Duke University Medical Center, Box 3389, Durham, North Carolina 27710, USA
    J Orthop Res 25:578-92. 2007
    ..Notably, the use of a murine model will facilitate the use of genetically modified animals for the investigation of specific genes implicated in the pathology of posttraumatic arthritis...
  65. ncbi Repair response of the inner and outer regions of the porcine meniscus in vitro
    Alfred Hennerbichler
    Department of Surgery, Division of Orthopaedic Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA
    Am J Sports Med 35:754-62. 2007
    ..Tears of the outer vascularized zone have a greater potential for repair than do tears in the inner avascular region. Objective and..
  66. ncbi Zonal changes in the three-dimensional morphology of the chondron under compression: the relationship among cellular, pericellular, and extracellular deformation in articular cartilage
    Jae Bong Choi
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    J Biomech 40:2596-603. 2007
    ....
  67. ncbi The influence of oxygen tension on the induction of nitric oxide and prostaglandin E2 by mechanical stress in articular cartilage
    Beverley Fermor
    Department of Surgery, Division of Orthopaedic Surgery, Box 3093, Duke University Medical Center, NC 27710, USA
    Osteoarthritis Cartilage 13:935-41. 2005
    ..The objective of this study was to determine the influence of oxygen tension on the induction of NO and PGE(2) production in articular cartilage in response to mechanical stress...
  68. ncbi Effects of transforming growth factor beta1 and dexamethasone on the growth and chondrogenic differentiation of adipose-derived stromal cells
    Hani A Awad
    Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA
    Tissue Eng 9:1301-12. 2003
    ..The addition of dexamethasone appeared to amplify protein synthesis but had suppressive effects on proteoglycan synthesis and accumulation...
  69. ncbi Differential effects of static and dynamic compression on meniscal cell gene expression
    Maureen L Upton
    Department of Biomedical Engineering, Box 90281, 136 Hudson Hall, Duke University, Durham, NC 27708, USA
    J Orthop Res 21:963-9. 2003
    ....
  70. ncbi Measurement of intracellular strain on deformable substrates with texture correlation
    Christopher L Gilchrist
    Department of Biomedical Engineering and Surgery, Duke University, 136 Hudson Hall, Box 90281, Durham, NC 27708, USA
    J Biomech 40:786-94. 2007
    ....
  71. ncbi The micromechanical environment of intervertebral disc cells determined by a finite deformation, anisotropic, and biphasic finite element model
    Anthony E Baer
    Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA
    J Biomech Eng 125:1-11. 2003
    ..Furthermore, the model predicted that micromechanical environment is strongly influenced by cell geometry, suggesting that the geometry of IVD cells in situ may be an adaptation to reduce cellular strains during tissue loading...
  72. ncbi Hyperosmotically induced volume change and calcium signaling in intervertebral disk cells: the role of the actin cytoskeleton
    Scott Pritchard
    Department of Surgery, Duke University Medical Center, 374 Medical Sciences Research Building, Durham, NC 27710, USA
    Biophys J 83:2502-10. 2002
    ..These results suggest that hyperosmotic stress induces volume change in IVD cells and may initiate [Ca(2+)](i) transients through an actin-dependent mechanism...
  73. ncbi Biaxial strain effects on cells from the inner and outer regions of the meniscus
    Maureen L Upton
    Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA
    Connect Tissue Res 47:207-14. 2006
    ..The results suggest that inner and outer meniscus cells may respond similarly to biaxial stretch in vitro with measures of biosynthesis and gene expression...
  74. ncbi Influence of oxygen on the proliferation and metabolism of adipose derived adult stem cells
    David W Wang
    Department of Surgery, Division of Orthopaedic Surgery, Duke University Medical Center, Durham, North Carolina, USA
    J Cell Physiol 204:184-91. 2005
    ....
  75. ncbi Anabolic effects of a G protein-coupled receptor kinase inhibitor expressed in osteoblasts
    Robert F Spurney
    Division of Nephrology, Department of Medicine, Duke University and Durham Veterans Administration Medical Centers, North Carolina 27710, USA
    J Clin Invest 109:1361-71. 2002
    ..The predominant effect of the transgene, however, was anabolic, as evidenced by an increase in bone density and trabecular bone volume in the transgenic mice compared with nontransgenic littermate controls...
  76. ncbi Observations on the growth of loose bodies in joints
    David E Attarian
    Division of Orthopaedic Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA
    Arthroscopy 18:930-4. 2002
    ..The loose bodies possess a large number of viable cells; and growth appears to have occurred through encapsulation of the niduses by multiple layers of fibrocartilagenous tissue, thus confirming the findings of earlier studies...
  77. ncbi Finite element modeling predictions of region-specific cell-matrix mechanics in the meniscus
    Maureen L Upton
    Department of Biomedical Engineering, Duke University, 136 Hudson Hall, Durham, NC 27708, USA
    Biomech Model Mechanobiol 5:140-9. 2006
    ....
  78. ncbi Induction of cyclooxygenase-2 by mechanical stress through a nitric oxide-regulated pathway
    B Fermor
    Department of Surgery, Division of Orthopaedic Surgery, Duke University Medical Center, NC 27710, USA
    Osteoarthritis Cartilage 10:792-8. 2002
    ....
  79. ncbi Regional material properties of the human hip joint capsule ligaments
    J Hewitt
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    J Orthop Res 19:359-64. 2001
    ..Furthermore, knowledge of the normal mechanical function of the hip capsule ligaments could assist in the evaluation of the success of a repair...
  80. ncbi A method for quantifying cell size from differential interference contrast images: validation and application to osmotically stressed chondrocytes
    L G Alexopoulos
    Orthopaedic Research Laboratories, Department of Surgery, Box 3093, Duke University Medical Center, Durham, NC 27710, USA
    J Microsc 205:125-35. 2002
    ..9% overall volume error without taking into account the error introduced by the representation of the cell as a sphere. As a test case, the method was applied to quantify volume changes due to acute changes of osmotic stress...
  81. ncbi Viability and apoptosis of human chondrocytes in osteochondral fragments following joint trauma
    W C Hembree
    Duke University Medical Center, Box 3389, Durham, North Carolina 27710, USA
    J Bone Joint Surg Br 89:1388-95. 2007
    ..These findings indicate the presence of both necrotic and apoptotic chondrocytes after joint injury and may provide further insight into the role of chondrocyte death in post-traumatic arthritis...
  82. ncbi Composition and transport properties of human ankle and knee cartilage
    Nicole L Fetter
    Department of Surgery, Division of Orthopaedic Surgery, Box 2923, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Orthop Res 24:211-9. 2006
    ....
  83. ncbi Regional differences in prostaglandin E2 and nitric oxide production in the knee meniscus in response to dynamic compression
    Alfred Hennerbichler
    Department of Surgery, Division of Orthopaedic Surgery, Duke University Medical Center, Box 3093, 375 Medical Sciences Research Building, Durham, NC 27710, USA
    Biochem Biophys Res Commun 358:1047-53. 2007
    ....
  84. ncbi Potent induction of chondrocytic differentiation of human adipose-derived adult stem cells by bone morphogenetic protein 6
    Bradley T Estes
    Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA
    Arthritis Rheum 54:1222-32. 2006
    ..Combinations of growth factors containing BMP-6 may provide a novel means of regulating the differentiation of ADAS cells for applications in the tissue-engineered repair or regeneration of articular cartilage...
  85. ncbi Ligament-derived matrix stimulates a ligamentous phenotype in human adipose-derived stem cells
    Dianne Little
    Division of Orthopaedic Surgery, Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA
    Tissue Eng Part A 16:2307-19. 2010
    ..05). These findings suggest that LDM can stimulate a ligament phenotype by hASCs, and may provide a novel scaffold material for ligament engineering applications...
  86. ncbi Compressive properties of mouse articular cartilage determined in a novel micro-indentation test method and biphasic finite element model
    Li Cao
    Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA
    J Biomech Eng 128:766-71. 2006
    ..This study provides the first available data for biphasic compressive properties in mouse cartilage and suggests a promising method for detecting altered cartilage mechanics in small animal models of osteoarthritis...
  87. ncbi Viscoelastic properties of human mesenchymally-derived stem cells and primary osteoblasts, chondrocytes, and adipocytes
    Eric M Darling
    Department of Surgery, Duke University Medical Center Durham, NC 27710, USA
    J Biomech 41:454-64. 2008
    ..Furthermore, the identification of mechanical properties distinct to stem cells could result in more successful sorting procedures to enrich multipotent progenitor cell populations...
  88. ncbi Regulation of matrix turnover in meniscal explants: role of mechanical stress, interleukin-1, and nitric oxide
    Sang-Jin Shin
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    J Appl Physiol 95:308-13. 2003
    ..These finding suggest that IL-1 may modulate the effects of mechanical stress on extracellular matrix turnover through a pathway that is dependent on NO...
  89. ncbi The effects of BMP6 overexpression on adipose stem cell chondrogenesis: Interactions with dexamethasone and exogenous growth factors
    Brian O Diekman
    Orthopaedic Research Laboratories, Department of Surgery, Division of Orthopaedic Surgery, 375 MSRB, Box 3093, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biomed Mater Res A 93:994-1003. 2010
    ..These results suggest that chondrogenic differentiation of ASCs depends on complex interactions among various growth factors and media supplements, as well as the concentration and duration of growth factor exposure...
  90. ncbi Site-specific effects of compression on macromolecular diffusion in articular cartilage
    Holly A Leddy
    Department of Surgery and Department of Biomedical Engineering, Duke University Medical Center, Durham, North Carolina 27710, USA
    Biophys J 95:4890-5. 2008
    ..Our findings suggest that high strains in the surface zone significantly decrease diffusivity and increase anisotropy, which may decrease transport between cartilage and synovial fluid during compression...
  91. ncbi Osteoarthritic changes in the biphasic mechanical properties of the chondrocyte pericellular matrix in articular cartilage
    Leonidas G Alexopoulos
    Orthopaedic Research Laboratories, Division of Orthopaedic Surgery, Department of Surgery, Duke University Medical Center, 375 Medical Sciences Research Building, Box 3093 Research Dr. Durham, NC 27710, USA
    J Biomech 38:509-17. 2005
    ....
  92. ncbi Molecular diffusion in tissue-engineered cartilage constructs: effects of scaffold material, time, and culture conditions
    Holly A Leddy
    Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biomed Mater Res B Appl Biomater 70:397-406. 2004
    ..The increase in diffusivity in the acellular constructs is probably due to scaffold degradation and swelling...
  93. ncbi Differentiation of adipose stem cells
    Bruce A Bunnell
    Department of Pharmacology, Center for Gene Therapy, Division of Gene Therapy, Tulane National Primate Research Center, Covington, LA, USA
    Methods Mol Biol 456:155-71. 2008
    ....
  94. ncbi Absence of posttraumatic arthritis following intraarticular fracture in the MRL/MpJ mouse
    Benjamin D Ward
    Duke University Medical Center, Durham, North Carolina 27710, USA
    Arthritis Rheum 58:744-53. 2008
    ..The MRL/MpJ mouse has shown unique regenerative abilities in response to injury. The objective of this study was to determine if the MRL/MpJ mouse is protected from posttraumatic arthritis after intraarticular fractures...
  95. ncbi Joint degeneration following meniscal allograft transplantation in a canine model: mechanical properties and semiquantitative histology of articular cartilage
    Dawn M Elliott
    McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, PA, USA
    Knee Surg Sports Traumatol Arthrosc 10:109-18. 2002
    ..Our findings do not support the hypothesis that meniscal allograft transplantation provides chondroprotection of the femoral condyle and also suggest that it does not lead to increased degenerative changes...
  96. ncbi From articular fracture to posttraumatic arthritis: a black box that needs to be opened
    Steven A Olson
    J Orthop Trauma 20:661-2. 2006
  97. ncbi Synovial fluid biomarker levels predict articular cartilage damage following complete medial meniscectomy in the canine knee
    Cathy S Carlson
    Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St Paul 55108, USA
    J Orthop Res 20:92-100. 2002
    ....
  98. ncbi Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo
    Geoffrey R Erickson
    Department of Surgery, Orthopaedic Research Laboratories, Duke University Medical Center, Durham, North Carolina 27710, USA
    Biochem Biophys Res Commun 290:763-9. 2002
    ....
  99. ncbi An in vitro system to evaluate the effects of ischemia on survival of cells used for cell therapy
    Bryce H Davis
    Department of Biomedical Engineering, Duke University, Durham, NC, USA
    Ann Biomed Eng 35:1414-24. 2007
    ..g., myoblasts, mesenchymal stem cells, and cardiac stem cells) and the impact of different treatment regimens on the likelihood of survival of transplanted cells...
  100. ncbi Chondropathy after meniscal tear or partial meniscectomy in a canine model
    Douglas J Wyland
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    J Orthop Res 20:996-1002. 2002
    ..These findings indicate that significant degeneration of canine articular cartilage develops to a similar degree in the presence of a partially healed meniscus tear or a PM of the knee...
  101. ncbi The slippery slope of arthritis
    Farshid Guilak
    Arthritis Rheum 52:1632-3. 2005

Research Grants28

  1. Intrinsic Repair of the Knee Meniscus
    Farshid Guilak; Fiscal Year: 2009
    ..Identification of these mechanisms will hopefully lead to new treatments that exploit optimal mechanical and biochemical modalities for the prevention of disease. ..
  2. VISCOELASTIC PROPERTIES OF NORMAL AND OA CHONDRONS
    Farshid Guilak; Fiscal Year: 2009
    ..A better understanding of these pathways will hopefully lead to the development of new pharmaceutical or biophysical interventions for the treatment of osteoarthritis. ..
  3. Mechanical and Osmotic Signaling in Chondrocytes
    Farshid Guilak; Fiscal Year: 2009
    ....
  4. Intrinsic Repair of the Knee Meniscus
    Farshid Guilak; Fiscal Year: 2009
    ..Identification of these mechanisms will hopefully lead to new treatments that exploit optimal mechanical and biochemical modalities for the prevention of disease. ..
  5. VISCOELASTIC PROPERTIES OF NORMAL AND OA CHONDRONS
    Farshid Guilak; Fiscal Year: 2010
    ..A better understanding of these pathways will hopefully lead to the development of new pharmaceutical or biophysical interventions for the treatment of osteoarthritis. ..
  6. Intrinsic Repair of the Knee Meniscus
    Farshid Guilak; Fiscal Year: 2010
    ..Identification of these mechanisms will hopefully lead to new treatments that exploit optimal mechanical and biochemical modalities for the prevention of disease. ..
  7. Mechanical and Osmotic Signaling in Chondrocytes
    Farshid Guilak; Fiscal Year: 2010
    ....
  8. Training in Biomolecular and Tissue Engineering
    Farshid Guilak; Fiscal Year: 2007
    ..We will also take advantage of several long-standing and successful initiatives at Duke University to enhance the recruitment of underrepresented minorities as part of this post-doctoral training program. ..
  9. VISCOELASTIC PROPERTIES OF NORMAL AND OA CHONDRONS
    Farshid Guilak; Fiscal Year: 2007
    ..A better understanding of these pathways will hopefully lead to the development of new pharmaceutical or biophysical interventions for the treatment of osteoarthritis. ..
  10. VISCOELASTIC PROPERTIES OF NORMAL AND OA CHONDRONS
    Farshid Guilak; Fiscal Year: 2002
    ..Identification of these mechanisms will hopefully lead to new treatments which exploit optimal mechanical and biochemical modalities for the prevention of osteoarthritis. ..
  11. VISCOELASTIC PROPERTIES OF NORMAL AND OA CHONDRONS
    Farshid Guilak; Fiscal Year: 2003
    ..A better understanding of these pathways will hopefully lead to the development of new pharmaceutical or biophysical interventions for the treatment of osteoarthritis. ..
  12. 3D Woven Scaffolds for Tissue Engineering
    Farshid Guilak; Fiscal Year: 2003
    ..An improved level of biomechanical function will increase the level of success in the engineered repair of various tissues of the musculoskeletal system as well as other organ systems of the body. ..
  13. Biomechanical Factors in Rheumatoid Arthritis
    Farshid Guilak; Fiscal Year: 2005
    ..abstract_text> ..
  14. VISCOELASTIC PROPERTIES OF NORMAL AND OA CHONDRONS
    Farshid Guilak; Fiscal Year: 2006
    ..A better understanding of these pathways will hopefully lead to the development of new pharmaceutical or biophysical interventions for the treatment of osteoarthritis. ..
  15. Mechanical and Osmotic Signaling in Chondrocytes
    Farshid Guilak; Fiscal Year: 2010
    ....