Research Topics
Species | John ChuteSummaryAffiliation: Duke University Medical Center Country: USA Publications
Research Grants
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Detail Information
Publications
Transplantation of vascular endothelial cells mediates the hematopoietic recovery and survival of lethally irradiated miceJohn P Chute
Division of Cellular Therapy, Duke University Medical Center, Durham, NC 27710, USA
Blood 109:2365-72. 2007..These data suggest that reestablishment of endothelial cell activity can improve the hematopoietic recovery and survival of irradiated mice...- Minireview: Nuclear receptors, hematopoiesis, and stem cellsJohn P Chute
Division of Cellular Therapy, Department of Medicine, Duke University, Durham, North Carolina 27710, USA
Mol Endocrinol 24:1-10. 2010..Herein, we review in detail the function of specific NRs in controlling HSC and other stem cell fate and propose a framework through which these observations can be translated into therapeutic amplification of HSCs for clinical purposes... 
Soluble factors elaborated by human brain endothelial cells induce the concomitant expansion of purified human BM CD34+CD38- cells and SCID-repopulating cellsJohn P Chute
Stem Cell Biology Laboratory, Large Scale Biology Corporation, Vacaville, CA, USA
Blood 105:576-83. 2005..These studies also demonstrate that HUBEC-elaborated soluble factors mediate a unique and potent expansion of human HSCs...- Molecular profile and partial functional analysis of novel endothelial cell-derived growth factors that regulate hematopoiesisJohn P Chute
Division of Cellular Therapy, Department of Internal Medicine, Duke University, Durham, North Carolina 27710, USA
Stem Cells 24:1315-27. 2006..These data demonstrate the potential of primary HUBECs as a reservoir for the discovery of novel secreted proteins that regulate human hematopoiesis... - A metabolic study of patients with lung cancer and hyponatremia of malignancyJohn P Chute
Department of Medicine, Duke University, Durham, North Carolina 27710, USA
Clin Cancer Res 12:888-96. 2006..We sought to distinguish patients with hyponatremia caused by elevated AVP versus those with ectopic atrial natriuretic peptide (ANP) via this detailed metabolic study... - Stem cell homingJohn P Chute
Division of Cellular Therapy, Duke University Medical Center, Durham, North Carolina 27710, USA
Curr Opin Hematol 13:399-406. 2006..This review discusses the latest progress in defining the molecular processes underlying stem cell homing and the specialized niches where stem cells reside... - Inhibition of aldehyde dehydrogenase and retinoid signaling induces the expansion of human hematopoietic stem cellsJohn P Chute
Division of Cellular Therapy, Duke University Medical Center, Durham, NC 27710, USA
Proc Natl Acad Sci U S A 103:11707-12. 2006..Modulation of ALDH activity and retinoid signaling is a previously unrecognized and effective strategy to amplify human HSCs... - Myeloablative intravenous busulfan/fludarabine conditioning does not facilitate reliable engraftment of dual umbilical cord blood grafts in adult recipientsMitchell E Horwitz
Adult Stem Cell Transplant Program, Division of Cellular Therapy, Duke University School of Medicine, Durham, NC 27710, USA
Biol Blood Marrow Transplant 14:591-4. 2008..We conclude that the myeloablative conditioning regimen of once-daily intravenous busulfan with fludarabine provides insufficient immunosuppression to allow for engraftment of partially matched, dual umbilical cord blood grafts... 
Natural killer cell-enriched donor lymphocyte infusions from A 3-6/6 HLA matched family member following nonmyeloablative allogeneic stem cell transplantationDavid A Rizzieri
Duke University Medical Center, Department of Medicine, Division of Cellular Therapy, Durham, North Carloina, 27705, USA
Biol Blood Marrow Transplant 16:1107-14. 2010..Future studies must assess whether the improved outcomes are the direct result of the high doses and improved NK cell function or other aspects of immune recovery...- Vascular endothelial cells produce soluble factors that mediate the recovery of human hematopoietic stem cells after radiation injuryGarrett G Muramoto
Division of Cellular Therapy, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
Biol Blood Marrow Transplant 12:530-40. 2006.... - Feasibility of low-dose interleukin-2 therapy following T-cell-depleted nonmyeloablative allogeneic hematopoietic stem cell transplantation from HLA-matched or -mismatched family member donorsDavid A Rizzieri
Department of Medicine, Division of Cellular Therapy, Duke University Medical Center, Durham, North Carolina 27710, USA
Cancer Invest 29:56-61. 2011..The primary objective of this pilot study was to evaluate the feasibility of administering IL-2 following a T-cell-depleted nonmyeloablative hematopoietic stem cell transplant... - Bone marrow CD34+ cells expanded on human brain endothelial cells reconstitute lethally irradiated baboons in a variable mannerHiroto Araki
Section of Hematology Oncology, Department of Medicine, University of Illinois at Chicago, IL, USA
Leuk Lymphoma 51:1121-7. 2010.... - Inhibition of aldehyde dehydrogenase expands hematopoietic stem cells with radioprotective capacityGarrett G Muramoto
Division of Cellular Therapy, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
Stem Cells 28:523-34. 2010..Pharmacologic inhibition of ALDH has therapeutic potential as a means to amplify ST-HSCs for transplantation purposes... - Ex vivo culture with human brain endothelial cells increases the SCID-repopulating capacity of adult human bone marrowJohn P Chute
Stem Cell Biology Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health NIH, Navy Transplantation and Autoimmunity Branch, Bethesda, MD, USA
Blood 100:4433-9. 2002..These data demonstrate that human brain endothelial cells possess a unique hematopoietic activity that increases the repopulating capacity of adult human bone marrow... - Endothelial progenitor cell infusion induces hematopoietic stem cell reconstitution in vivoAlice B Salter
Division of Cellular Therapy, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
Blood 113:2104-7. 2009..These data demonstrate that allogeneic EPC infusions can augment hematopoiesis and suggest a relationship between BM microvascular recovery and hematopoietic reconstitution in vivo... - Renal shielding and dosimetry for patients with severe systemic sclerosis receiving immunoablation with total body irradiation in the scleroderma: cyclophosphamide or transplantation trialOana I Craciunescu
Department of Radiation Oncology, Duke University Medical Center, Durham, NC 27710, USA
Int J Radiat Oncol Biol Phys 79:1248-55. 2011..To describe renal shielding techniques and dosimetry in delivering total body irradiation (TBI) to patients with severe systemic sclerosis (SSc) enrolled in a hematopoietic stem cell transplant protocol... - Fludarabine-based nonmyeloablative stem cell transplantation for sickle cell disease with and without renal failure: clinical outcome and pharmacokineticsMitchell E Horwitz
Adult Stem Cell Transplant Program, Division of Cellular Therapy, Duke University School of Medicine, Durham, North Carolina 27710, USA
Biol Blood Marrow Transplant 13:1422-6. 2007..We conclude that fludarabine-based nonmyeloablative allogeneic SCT for adult patients with SCD is feasible, even in the setting of ESRD... - Pharmacological manipulation of the RAR/RXR signaling pathway maintains the repopulating capacity of hematopoietic stem cells in cultureRachid Safi
Department of Pharmacology and Cancer Biology, Duke University, Durham, North Carolina 27710, USA
Mol Endocrinol 23:188-201. 2009..These data indicate that the RAR/RXR heterodimer is a critical regulator of human HSC differentiation, and pharmacological modulation of RXR signaling prevents the loss of human HSCs that otherwise occurs in short-term culture... - Pleiotrophin regulates the expansion and regeneration of hematopoietic stem cellsHeather A Himburg
Division of Cellular Therapy, Department of Medicine, Duke University, Durham, North Carolina, USA
Nat Med 16:475-82. 2010..We identify the secreted growth factor pleiotrophin as a new regulator of both HSC expansion and regeneration... - "Short course" bortezomib plus melphalan and prednisone as induction prior to transplant or as frontline therapy for nontransplant candidates in patients with previously untreated multiple myelomaCristina Gasparetto
Department of Cellular Therapy, Duke University Medical Center, Durham, NC 27710, USA
Biol Blood Marrow Transplant 16:70-7. 2010..VMP did not negatively affect stem cell collection. Longer follow-up and prospective phase III trials are required to validate these initial observations... - Growth hormone mitigates against lethal irradiation and enhances hematologic and immune recovery in mice and nonhuman primatesBenny J Chen
Department of Medicine, Duke University Medical Center, Durham, North Carolina, United States of America
PLoS ONE 5:e11056. 2010..Our data demonstrate that rhGH promotes hematopoietic engraftment and immune recovery post the exposure of ionizing radiation and mitigates against the mortality from lethal irradiation even when administered after exposure... - Ex vivo culture rescues hematopoietic stem cells with long-term repopulating capacity following harvest from lethally irradiated miceJohn P Chute
Large Scale Biology Corporation, Vacaville, Calif, USA
Exp Hematol 32:308-17. 2004..High-dose ionizing radiation can cause lethal myeloablation in exposed individuals. We examined whether ex vivo culture could rescue hematopoietic stem cells with repopulating capacity following harvest from lethally irradiated animals... - Quantitative analysis demonstrates expansion of SCID-repopulating cells and increased engraftment capacity in human cord blood following ex vivo culture with human brain endothelial cellsJohn P Chute
Stem Cell Biology Laboratory, Large Scale Biology Corporation, Vacaville, California, USA
Stem Cells 22:202-15. 2004..These studies indicate that the application of HUBEC coculture or HUBEC-conditioned media can potentially improve upon current strategies for the clinical expansion of CB stem cells... 
Gene expression signatures that predict radiation exposure in mice and humansHolly K Dressman
Institute for Genome Sciences and Policy, Duke University Medical Center, Durham, North Carolina, United States of America
PLoS Med 4:e106. 2007..The capacity to assess environmental inputs to biological phenotypes is limited by methods that can accurately and quantitatively measure these contributions. One such example can be seen in the context of exposure to ionizing radiation...- Radiation injury treatment network (RITN): healthcare professionals preparing for a mass casualty radiological or nuclear incidentJoel R Ross
Division of Cellular Therapy, Duke University, Durham, NC 27710, USA
Int J Radiat Biol 87:748-53. 2011..CONCLUSION: RITN serves as a vital partner in preparedness and response efforts for potential radiological and nuclear incidents... - Diagnosis of partial body radiation exposure in mice using peripheral blood gene expression profilesSarah K Meadows
Division of Cellular Therapy, Department of Medicine, Duke University, Durham, North Carolina, United States of America
PLoS ONE 5:e11535. 2010.... - Partially matched, nonmyeloablative allogeneic transplantation: clinical outcomes and immune reconstitutionDavid A Rizzieri
Department of Medicine, Division of Cellular Therapy, Duke University Medical Center, Durham, NC 27710, USA
J Clin Oncol 25:690-7. 2007..To allow a donor to be found for nearly all patients, we have used a nonmyeloablative conditioning regimen in conjunction with stem cells from a related donor with one fully mismatched HLA haplotype... - Hedgehog signalling is essential for maintenance of cancer stem cells in myeloid leukaemiaChen Zhao
Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
Nature 458:776-9. 2009.... - Gene expression signatures of radiation response are specific, durable and accurate in mice and humansSarah K Meadows
Division of Cellular Therapy, Duke University Medical Center, Durham, North Carolina, United States of America
PLoS ONE 3:e1912. 2008..Previous work has demonstrated the potential for peripheral blood (PB) gene expression profiling for the detection of disease or environmental exposures... - Nanoscale detection of ionizing radiation damage to DNA by atomic force microscopyChanghong Ke
Center for Biologically Inspired Materials and Material Systems and Department of Mechanical, Engineering and Materials Science, Duke University, Durham, NC 27708, USA
Small 4:288-94. 2008..01 ng microL(-1)), which is beyond the capability of traditional techniques. AFM imaging can uniquely supplement traditional techniques for sensitive measurements of damage to DNA by ionizing radiation... - Rescue of hematopoietic stem cells following high-dose radiation injury using ex vivo culture on endothelial monolayersJohn P Chute
Navy/National Institute of Diabetes and Digestive and Kidney Diseases-Transplantation and Autoimmunity Branch, Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
Mil Med 167:74-7. 2002..1%; mean, 1.9%). These results demonstrate that a subpopulation of bone marrow stem cells are capable of surviving the effects of high-dose radiation if these cells are placed in coculture with endothelial cell monolayers...
Research Grants
- Endothelial Progenitor Cell Transplant to Accelerate Hematopoietic RecoveryJohn P Chute; Fiscal Year: 2010....