Wei Chen

Summary

Affiliation: Duke University Medical Center
Country: USA

Publications

  1. ncbi request reprint Dishevelled 2 recruits beta-arrestin 2 to mediate Wnt5A-stimulated endocytosis of Frizzled 4
    Wei Chen
    Howard Hughes Medical Institute, Departments of Medicine and Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    Science 301:1391-4. 2003
  2. pmc G Protein-coupled receptor kinases phosphorylate LRP6 in the Wnt pathway
    Minyong Chen
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 284:35040-8. 2009
  3. pmc Regulation of hedgehog signaling by Myc-interacting zinc finger protein 1, Miz1
    Jiuyi Lu
    Department of Medicine, Duke University, Durham, North Carolina, USA
    PLoS ONE 8:e63353. 2013
  4. pmc Cadmium is a potent inhibitor of PPM phosphatases and targets the M1 binding site
    Chang Pan
    Key Laboratory Experimental Teratology of the Ministry of Education and Department of Biochemistry and Molecular Biology, Shandong University, School of Medicine, Jinan, Shandong 250012, China
    Sci Rep 3:2333. 2013
  5. pmc Development of small molecules targeting the Wnt pathway for the treatment of colon cancer: a high-throughput screening approach
    Wei Chen
    Dept of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Am J Physiol Gastrointest Liver Physiol 299:G293-300. 2010
  6. ncbi request reprint Activity-dependent internalization of smoothened mediated by beta-arrestin 2 and GRK2
    Wei Chen
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Science 306:2257-60. 2004
  7. ncbi request reprint Beta-arrestin 2 mediates endocytosis of type III TGF-beta receptor and down-regulation of its signaling
    Wei Chen
    Howard Hughes Medical Institute, Duke University Medical Center, Departments of Medicine and Biochemistry, Durham, NC 27710, USA
    Science 301:1394-7. 2003
  8. pmc Polyclonal HER2-specific antibodies induced by vaccination mediate receptor internalization and degradation in tumor cells
    Xiu Rong Ren
    Department of Medicine, Duke University Medical Center, 595 LaSalle Street, Durham, NC 27710, USA
    Breast Cancer Res 14:R89. 2012
  9. ncbi request reprint Effects of trichostatin A in a rat model of acute graft-versus-host disease after liver transplantation
    Xuefeng Xia
    Department of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
    Transplantation 96:25-33. 2013
  10. pmc Beta-arrestin-mediated localization of smoothened to the primary cilium
    Jeffrey J Kovacs
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Science 320:1777-81. 2008

Research Grants

Collaborators

Detail Information

Publications115 found, 100 shown here

  1. ncbi request reprint Dishevelled 2 recruits beta-arrestin 2 to mediate Wnt5A-stimulated endocytosis of Frizzled 4
    Wei Chen
    Howard Hughes Medical Institute, Departments of Medicine and Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    Science 301:1391-4. 2003
    ..These findings provide a previously unrecognized mechanism for receptor recruitment of beta-arrestin and demonstrate that Dvl plays an important role in the endocytosis of frizzled, as well as in promoting signaling...
  2. pmc G Protein-coupled receptor kinases phosphorylate LRP6 in the Wnt pathway
    Minyong Chen
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 284:35040-8. 2009
    ..Expression of GRK5 rescues the diminished beta-catenin and axin2 response caused by GRK5 depletion. Thus, our findings identify GRK5/6 as novel kinases for the single transmembrane receptor LRP6 during Wnt signaling...
  3. pmc Regulation of hedgehog signaling by Myc-interacting zinc finger protein 1, Miz1
    Jiuyi Lu
    Department of Medicine, Duke University, Durham, North Carolina, USA
    PLoS ONE 8:e63353. 2013
    ..Taken together, these results identify Miz1 as a novel regulator in the Hh pathway that plays an important role in mediating Smo-dependent oncogenic signaling...
  4. pmc Cadmium is a potent inhibitor of PPM phosphatases and targets the M1 binding site
    Chang Pan
    Key Laboratory Experimental Teratology of the Ministry of Education and Department of Biochemistry and Molecular Biology, Shandong University, School of Medicine, Jinan, Shandong 250012, China
    Sci Rep 3:2333. 2013
    ..Taken together, these findings provide a better understanding of the effects of the toxicity of cadmium in the contexts of human physiology and pathology. ..
  5. pmc Development of small molecules targeting the Wnt pathway for the treatment of colon cancer: a high-throughput screening approach
    Wei Chen
    Dept of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Am J Physiol Gastrointest Liver Physiol 299:G293-300. 2010
    ..In particular, we summarize the results of a screen of over 1,200 drug and druglike compounds we recently completed in which niclosamide was identified as a Wnt pathway antagonist...
  6. ncbi request reprint Activity-dependent internalization of smoothened mediated by beta-arrestin 2 and GRK2
    Wei Chen
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Science 306:2257-60. 2004
    ..A Smo agonist stimulated and a Smo antagonist (cyclopamine) inhibited both phosphorylation of Smo by GRK2 and interaction of beta-arrestin 2 with Smo. beta-Arrestin 2 and GRK2 are thus potential mediators of signaling by activated Smo...
  7. ncbi request reprint Beta-arrestin 2 mediates endocytosis of type III TGF-beta receptor and down-regulation of its signaling
    Wei Chen
    Howard Hughes Medical Institute, Duke University Medical Center, Departments of Medicine and Biochemistry, Durham, NC 27710, USA
    Science 301:1394-7. 2003
    ..Thus, the regulatory actions of beta-arrestins are broader than previously appreciated, extending to the TGF-beta receptor family as well...
  8. pmc Polyclonal HER2-specific antibodies induced by vaccination mediate receptor internalization and degradation in tumor cells
    Xiu Rong Ren
    Department of Medicine, Duke University Medical Center, 595 LaSalle Street, Durham, NC 27710, USA
    Breast Cancer Res 14:R89. 2012
    ..Thus, abrogation of persistent HER2 expression at the plasma membrane to synergize with current approaches may represent a novel therapeutic strategy...
  9. ncbi request reprint Effects of trichostatin A in a rat model of acute graft-versus-host disease after liver transplantation
    Xuefeng Xia
    Department of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
    Transplantation 96:25-33. 2013
    ..Histone deacetylase inhibitors promote the production of Tregs and some, such as suberoylanilide hydroxamic acid and trichostatin A (TSA), are used to treat autoimmune diseases, including GVHD after bone marrow transplantation...
  10. pmc Beta-arrestin-mediated localization of smoothened to the primary cilium
    Jeffrey J Kovacs
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Science 320:1777-81. 2008
    ..These results suggest roles for beta-arrestins in mediating the intracellular transport of a 7TMR to its obligate subcellular location for signaling...
  11. pmc Growth Arrest Specific 8 (Gas8) and G protein-coupled receptor kinase 2 (GRK2) cooperate in the control of Smoothened signaling
    Tama Evron
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 286:27676-86. 2011
    ..This is in contrast to cells stably expressing both GRK2 and Smo, in which cilia are significantly elongated. These results identify Gas8 as a positive regulator of Hh signaling that cooperates with GRK2 to control Smo signaling...
  12. doi request reprint Role of hepatic stellate cells on graft injury after small-for-size liver transplantation
    Wei Chen
    Department of Hepatobiliary and Pancreatic Surgery, Key Laboratory of Combined Multi organ Transplantation of Ministry of Public Health, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
    J Gastroenterol Hepatol 26:1659-68. 2011
    ..Hepatic stellate cells activate and trans-differentiate into contractile myofibroblast-like cells during liver injury. However, the role of hepatic stellate cells on sinusoidal microcirculation is unknown with small-for-size grafts...
  13. pmc Different G protein-coupled receptor kinases govern G protein and beta-arrestin-mediated signaling of V2 vasopressin receptor
    Xiu Rong Ren
    Department of Biochemistry, Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 102:1448-53. 2005
    ..GRK2 suppression actually increased beta-arrestin-stimulated ERK activation. These results suggest that beta-arrestin recruited in response to receptor phosphorylation by different GRKs has distinct functional potentials...
  14. ncbi request reprint GIPC interacts with the beta1-adrenergic receptor and regulates beta1-adrenergic receptor-mediated ERK activation
    Liaoyuan A Hu
    Howard Hughes Medical Institute, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 278:26295-301. 2003
    ..These data suggest that GIPC can regulate beta1-adrenergic receptor-stimulated, Gi-mediated, ERK activation while having no effect on receptor internalization or Gs-mediated cAMP signaling...
  15. pmc ATP production rate via creatine kinase or ATP synthase in vivo: a novel superfast magnetization saturation transfer method
    Qiang Xiong
    Cardiovascular Division, Department of Medicine, University of Minnesota, Minneapolis, USA
    Circ Res 108:653-63. 2011
    ..However, its lengthy data acquisition time greatly limits the wide biomedical applications in vivo, especially for studies requiring high spatial and temporal resolutions...
  16. pmc Identification of select glucocorticoids as Smoothened agonists: potential utility for regenerative medicine
    Jiangbo Wang
    Departments of Medicine, Surgery, and Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 107:9323-8. 2010
    ..Halcinonide, fluticasone, clobetasol, and fluocinonide provide an unprecedented opportunity to develop unique clinical strategies to treat Hedgehog-dependent illnesses...
  17. pmc Paracrine modulation of cholangiocyte serotonin synthesis orchestrates biliary remodeling in adults
    Alessia Omenetti
    Duke Univ Medical Center Division of Gastroenterology, GSRB 1, 595 LaSalle St, Suite 1073, Box 3256 Durham, NC 27710, USA
    Am J Physiol Gastrointest Liver Physiol 300:G303-15. 2011
    ..Given the prevalent use of serotonin-modulating drugs, these findings have potentially important implications for recovery from various types of liver damage...
  18. ncbi request reprint Sustained activation of Rac1 in hepatic stellate cells promotes liver injury and fibrosis in mice
    Steve S Choi
    Division of Gastroenterology and Department of Medicine, Duke University Medical Center, Durham, NC, USA
    Hepatology 44:1267-77. 2006
    ..In conclusion, sustained activation of Rac in HSCs perpetuates their activation and exacerbates toxin-induced liver injury and fibrosis, prompting speculation that Rac may be a therapeutic target in patients with cirrhosis...
  19. ncbi request reprint Phosphorylation of beta-arrestin2 regulates its function in internalization of beta(2)-adrenergic receptors
    Fang Tsyr Lin
    Howard Hughes Medical Institute and Departments of Medicine and Biochemistry, Duke University Medical Center, Box 3821, Durham, NC 27710, USA
    Biochemistry 41:10692-9. 2002
    ..Thus it appears that the function of all arrestin family members in mediating internalization of G protein-coupled receptors is regulated by distinct phosphorylation/dephosphorylation mechanisms...
  20. pmc The anti-helminthic niclosamide inhibits Wnt/Frizzled1 signaling
    Minyong Chen
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Biochemistry 48:10267-74. 2009
    ..e., Frizzled and Dishevelled) and moreover may provide a valuable means of studying the physiological consequences of Wnt signaling...
  21. doi request reprint NSC 74859-mediated inhibition of STAT3 enhances the anti-proliferative activity of cetuximab in hepatocellular carcinoma
    Wei Chen
    Department of Hepatobiliary and Pancreatic Surgery, Key Laboratory of Combined Multi organ Transplantation of Ministry of Public Health, The First Affiliated Hospital, School of Medicine, Zhejiang University, China
    Liver Int 32:70-7. 2012
    ..STAT3, which is thought to be a determinant of HCC sensitivity to antitumour drugs, may be involved...
  22. pmc The insecticide synergist piperonyl butoxide inhibits hedgehog signaling: assessing chemical risks
    Jiangbo Wang
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    Toxicol Sci 128:517-23. 2012
    ....
  23. pmc Fibroblasts in post-infarction inflammation and cardiac repair
    Wei Chen
    Department of Medicine, Albert Einstein College of Medicine, Bronx NY, USA
    Biochim Biophys Acta 1833:945-53. 2013
    ..This article is part of a Special Issue entitled: Cardiomyocyte Biology: Cardiac Pathways of Differentiation, Metabolism and Contraction...
  24. pmc Antihelminth compound niclosamide downregulates Wnt signaling and elicits antitumor responses in tumors with activating APC mutations
    Takuya Osada
    Department of Surgery, Duke University Medical Center, Durham, North Carolina 27705, USA
    Cancer Res 71:4172-82. 2011
    ..Our findings support clinical explorations to reposition niclosamide for the treatment of CRC...
  25. pmc Smoothened signaling in vertebrates is facilitated by a G protein-coupled receptor kinase
    Melanie Philipp
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Mol Biol Cell 19:5478-89. 2008
    ..Together, these data suggest that a GRK functions as a vertebrate kinase for Smoothened, promoting Hedgehog signal transduction during early development...
  26. doi request reprint Mesenchymal stem cells administered after liver transplantation prevent acute graft-versus-host disease in rats
    Xuefeng Xia
    Department of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China
    Liver Transpl 18:696-706. 2012
    ..In conclusion, the post-LT administration of MSCs of either donor or recipient origin could prevent the onset of LT-aGVHD in our rat model...
  27. ncbi request reprint G protein-coupled receptor kinase 5 regulates beta 1-adrenergic receptor association with PSD-95
    Liaoyuan A Hu
    Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:1607-13. 2002
    ..Regulation of protein association through receptor phosphorylation may be a general mechanism used by G protein-coupled receptors that associate via PDZ domain-mediated protein/protein interactions...
  28. pmc Increased production of sonic hedgehog by ballooned hepatocytes
    Fatima Rangwala
    Division of Cellular Therapy, Hematology and Oncology, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    J Pathol 224:401-10. 2011
    ..These results help to explain why fibrosis stage correlates with hepatocyte ballooning in NASH...
  29. doi request reprint A novel anticancer effect of butein: inhibition of invasion through the ERK1/2 and NF-kappa B signaling pathways in bladder cancer cells
    Lirui Zhang
    Center for Laboratory Medicine, The First Affiliated Hospital, School of Medicine, Xi an Jiaotong University, Xi an, China
    FEBS Lett 582:1821-8. 2008
    ..These results suggest a novel function of butein as an invasion inhibitor in bladder cancer...
  30. pmc Small protease sensitive oligomers of PrPSc in distinct human prions determine conversion rate of PrP(C)
    Chae Kim
    National Prion Disease Surveillance Center, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA
    PLoS Pathog 8:e1002835. 2012
    ....
  31. pmc Estrogen improves the hyperdynamic circulation and hyporeactivity of mesenteric arteries by alleviating oxidative stress in partial portal vein ligated rats
    Bin Zhang
    Bin Zhang, Cheng Gang Zhang, Quan Bo Zhou, Wei Chen, Zhi Yong Wu, Department of General Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China
    World J Gastroenterol 19:6863-8. 2013
    ..To evaluate the effects of estrogen (E2) on systemic and splanchnic hyperdynamic circulation in portal hypertensive rats...
  32. pmc Small molecule modulators of Wnt/β-catenin signaling
    Robert A Mook
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Bioorg Med Chem Lett 23:2187-91. 2013
    ..Overall, these findings support efforts to identify the target of Niclosamide inhibition of Wnt/β-catenin signaling and the discovery of potent and selective modulators to treat human disease...
  33. pmc Identification of a novel Smoothened antagonist that potently suppresses Hedgehog signaling
    Jiangbo Wang
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Bioorg Med Chem 20:6751-7. 2012
    ..Our data validate this novel screening approach in the further development of A8 and related congeners to treat Hedgehog related diseases, including the treatment of basal cell carcinoma and medulloblastoma...
  34. doi request reprint UCA1, a non-protein-coding RNA up-regulated in bladder carcinoma and embryo, influencing cell growth and promoting invasion
    Fan Wang
    Center for Molecular Medicine, The First Affiliated Hospital, School of Medicine, Xi an Jiaotong University, Xi an, China
    FEBS Lett 582:1919-27. 2008
    ..Moreover, the expression of UCA1 also increased tumorigenic potential of BLS-211 cells in nude mice. Results from the present study suggested that UCA1 might play a pivotal role in bladder cancer progression and embryonic development...
  35. pmc Phase 1 clinical trial of HER2-specific immunotherapy with concomitant HER2 kinase inhibition [corrected]
    Erika Hamilton
    Department of Surgery, Division of General Surgery, Duke University Medical Center, Durham, NC, USA
    J Transl Med 10:28. 2012
    ..We wished to test the clinical safety, immunogenicity, and activity of a HER2-based cancer vaccine, when combined with lapatinib...
  36. ncbi request reprint Desensitization of G-protein-coupled receptors induces vascular hypocontractility in response to norepinephrine in the mesenteric arteries of cirrhotic patients and rats
    Wei Chen
    Department of Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, China
    Hepatobiliary Pancreat Dis Int 12:295-304. 2013
    ..The present study is to investigate the machinery changes of alpha-adrenergic receptors and G proteins and their roles in the contractility of mesenteric arteries of cirrhotic patients and animal models...
  37. pmc Ets-2 regulates cell apoptosis via the Akt pathway, through the regulation of urothelial cancer associated 1, a long non-coding RNA, in bladder cancer cells
    Wenjing Wu
    Clinical Laboratory, The First Affiliated Hospital, School of Medicine, Xi an Jiaotong University, Xi an, China
    PLoS ONE 8:e73920. 2013
    ....
  38. ncbi request reprint Regulation of ALK-1 signaling by the nuclear receptor LXRbeta
    Jinyao Mo
    Department of Medicine, Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 277:50788-94. 2002
    ....
  39. ncbi request reprint EIF5A2 is a novel chemoresistance gene in breast cancer
    Yu Liu
    Breast Center of the First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China
    Breast Cancer . 2014
    ..EIF5A2 overexpression correlates significantly with tumor metastasis and is an adverse prognostic marker. However, eIF-5A2 overexpression in breast cancer and its effect on chemotherapy are unknown...
  40. pmc On Bayesian methods of exploring qualitative interactions for targeted treatment
    Wei Chen
    Department of Oncology, School of Medicine, Wayne State University, Detroit, MI 48201, USA
    Stat Med 31:3693-707. 2012
    ..We illustrate this method in two examples of phase III clinical trials. The algorithm is straightforward and easy to implement using existing software packages. We provide a sample code in Appendix A...
  41. pmc Triphenylmethane dye activation of beta-arrestin
    Larry S Barak
    Departments of Cell Biology, Duke University, Durham, NC 27710, USA
    Biochemistry 52:5403-14. 2013
    ..S. and Europe. Our results indicate triphenylmethane dyes as a result of novel pharmacology may have additional roles as β-arrestin/clathrin pathway signaling modulators in both pharmacology research and clinical therapy. ..
  42. pmc Synergism from combined immunologic and pharmacologic inhibition of HER2 in vivo
    Michael A Morse
    Division of Medical Oncology, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Int J Cancer 126:2893-903. 2010
    ..Based on these results, we feel clinical studies using this approach to target HER2-overexpressing breast cancer, including trastuzumab- and lapatinib-resistant tumors is warranted...
  43. pmc Tetrahydrobiopterin deficiency and nitric oxide synthase uncoupling contribute to atherosclerosis induced by disturbed flow
    Li Li
    Division of Cardiology, Department of Medicine, Emory University School of Medicine, Atlanta, GA, USA
    Arterioscler Thromb Vasc Biol 31:1547-54. 2011
    ..We therefore aimed to determine whether atherosclerosis induced by disturbed flow is due to BH(4) deficiency and NOS uncoupling and whether increasing BH(4) would prevent endothelial dysfunction, plaque inflammation, and atherosclerosis...
  44. pmc Novel strategy for measuring creatine kinase reaction rate in the in vivo heart
    Qiang Xiong
    Cardiovascular Division, Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota, USA
    Am J Physiol Heart Circ Physiol 297:H1010-9. 2009
    ..This method is validated using an in vivo pig model to evaluate the forward pseudo-first-order rate constant of myocardial CK reaction noninvasively...
  45. doi request reprint A novel high-throughput screening assay for discovery of molecules that increase cellular tetrahydrobiopterin
    Li Li
    The Division of Cardiology, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia, USA
    J Biomol Screen 16:836-44. 2011
    ..Thus, this TR-FRET assay could be applied in future uHTS of additional libraries to search for molecules that increase GTPCH-1 activity and BH(4) levels...
  46. pmc Liver cell-derived microparticles activate hedgehog signaling and alter gene expression in hepatic endothelial cells
    Rafal P Witek
    Department of Medicine, Duke University, Durham, North Carolina 27710, USA
    Gastroenterology 136:320-330.e2. 2009
    ..We studied whether adult liver cell-derived microparticles contain Hh ligands that alter hepatic sinusoidal endothelial cells (SEC)...
  47. pmc Human development index is associated with mortality-to-incidence ratios of gastrointestinal cancers
    Qi Da Hu
    Department of Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, Zhejiang Province, China
    World J Gastroenterol 19:5261-70. 2013
    ..To identify the role of human development in the incidence and mortality rates of gastrointestinal cancers worldwide...
  48. pmc Reactive oxygen species are involved in regulating hypocontractility of mesenteric artery to norepinephrine in cirrhotic rats with portal hypertension
    Wei Chen
    Department of Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China
    Int J Biol Sci 10:386-95. 2014
    ....
  49. doi request reprint Immunoscreening of urinary bladder cancer cDNA library and identification of potential tumor antigen
    Ling Chen
    Center for Clinical Molecular Biology, Department of Oncology, The First Affiliated Hospital, School of Medicine, Xi an Jiaotong University, Xi an, China
    World J Urol 27:107-12. 2009
    ..To identify potential tumor antigen by immunoscreening the urinary bladder cancer cDNA library with monoclonal antibodies...
  50. doi request reprint Long non-coding RNA UCA1 regulated cell cycle distribution via CREB through PI3-K dependent pathway in bladder carcinoma cells
    Chen Yang
    Center for Molecular Medicine, The First Affiliated Hospital, School of Medicine, Xi an Jiaotong University, 76 West Yanta Road, 710061 Xi an, China
    Gene 496:8-16. 2012
    ..Taken together, we concluded that UCA1 regulated cell cycle through CREB via PI3K-AKT dependent pathway in bladder cancer...
  51. pmc Expression of MMP-9 in hepatic sinusoidal obstruction syndrome induced by Gynura segetum
    Xia Zhen Yu
    Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China
    J Zhejiang Univ Sci B 14:68-75. 2013
    ..However, the mechanism is unclear. The aim was to study the role of matrix metalloproteinase-9 (MMP-9) in the onset of HSOS induced by Gynura segetum...
  52. pmc Systematic characterization of myocardial inflammation, repair, and remodeling in a mouse model of reperfused myocardial infarction
    Panagiota Christia
    Wilf Family Cardiovascular Research Institute, Department of Medicine, Division of Cardiology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Histochem Cytochem 61:555-70. 2013
    ..Rapid initiation and resolution of the inflammatory response, accelerated scar maturation, and extensive infarct volume loss are important characteristics of infarct healing in mice. ..
  53. ncbi request reprint Open-chest 31P magnetic resonance spectroscopy of mouse heart at 4.7 Tesla
    Joseph Lee
    Department of Medicine, University of Minnesota Academic Health Center, Minneapolis, Minnesota 55455, USA
    J Magn Reson Imaging 24:1269-76. 2006
    ..To develop a rapid, robust, and accurate method for assessing myocardial energetics in mice and demonstrate its applicability to mouse models of acquired and genetic heart disease...
  54. doi request reprint Advanced In Vivo Heteronuclear MRS Approaches for Studying Brain Bioenergetics Driven by Mitochondria
    Xiao Hong Zhu
    Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, School of Medicine, Minneapolis, MN, USA
    Methods Mol Biol 489:317-57. 2009
    ....
  55. pmc Identifying microRNA-mRNA regulatory network in colorectal cancer by a combination of expression profile and bioinformatics analysis
    Jihong Fu
    Colorectal Surgery Department, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Kongjiang Road 1665, Shanghai 200092, China
    BMC Syst Biol 6:68. 2012
    ..However, the miRNA-mRNA regulatory network is far from being fully understood. The objective of this study is to identify the colorectal cancer (CRC) specific miRNAs and their target mRNAs using a multi-step approach...
  56. doi request reprint Sinusoidal microcirculatory changes after small-for-size liver transplantation in rats
    Junjian Li
    Department of Hepatobiliary Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Key Laboratory of Multi Organ Transplantation of Ministry of Public Health, Hangzhou, China
    Transpl Int 23:924-33. 2010
    ..Long-term microcirculation disturbance displayed as decrease of sinusoidal reperfusion area and increase of spread in functional liver mass seems to be the key factor for graft injuries...
  57. pmc Correlation of Chimerism with Acute Graft-versus-Host Disease in Rats following Liver Transplantation
    Fei Xue
    Department of Hepatobiliary and Pancreatic Surgery, Key Laboratory of Combined Multi organ Transplantation of Ministry of Public Health, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China
    Int J Hepatol 2011:947150. 2011
    ..In conclusion, high level chimerism correlated well with LTx-aGVHD, and detection of chimerism soon after transplantation may be of value in the diagnosis of LTx-aGVHD prior to the onset of symptoms...
  58. doi request reprint Knockdown of dual specificity phosphatase 4 enhances the chemosensitivity of MCF-7 and MCF-7/ADR breast cancer cells to doxorubicin
    Yu Liu
    Department of Breast Surgery Center, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, Zhejiang Province, China
    Exp Cell Res 319:3140-9. 2013
    ..Dual specificity phosphatase 4 (DUSP4), a member of the dual specificity phosphatase family, is associated with cellular proliferation and differentiation; however, its role in breast cancer progression is controversial...
  59. doi request reprint Glucocorticoid hedgehog agonists in neurogenesis
    Jiangbo Wang
    Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA
    Vitam Horm 87:207-15. 2011
    ....
  60. pmc Calcium-dependent NOX5 nicotinamide adenine dinucleotide phosphate oxidase contributes to vascular oxidative stress in human coronary artery disease
    Tomasz J Guzik
    Division of Cardiology, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Am Coll Cardiol 52:1803-9. 2008
    ..This study sought to examine the expression and activity of the calcium-dependent nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) in human atherosclerotic coronary arteries...
  61. pmc PPARgamma agonists prevent TGFbeta1/Smad3-signaling in human hepatic stellate cells
    Caiyan Zhao
    Division of Gastroenterology, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Biochem Biophys Res Commun 350:385-91. 2006
    ..Thus, the anti-fibrotic actions of PPARgamma agonists reflect their ability to inhibit TGFbeta1-TGFbetaR1 signaling that initiates ECM gene expression in quiescent HSC...
  62. ncbi request reprint Regulation of endothelial cell tetrahydrobiopterin pathophysiological and therapeutic implications
    David G Harrison
    Department of Medicine, Division of Cardiology, Emory University School of Medicine, Atlanta Veterans Administration Medical Center, Decatur, Georgia, USA
    Adv Pharmacol 60:107-32. 2010
    ..Whether BH(4) or related agents will be useful in treatment of human diseases needs additional study...
  63. ncbi request reprint Role for hedgehog signaling in hepatic stellate cell activation and viability
    Jason K Sicklick
    Division of Gastroenterology and Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Lab Invest 85:1368-80. 2005
    ..Our findings reveal a novel mechanism, namely the Hh pathway, that regulates the activation and viability of HSC...
  64. doi request reprint Effects of add-on nebivolol on blood pressure and glucose parameters in hypertensive patients with prediabetes
    Prakash Deedwania
    Department of Medicine, UCSF School of Medicine, University of California San Francisco, CA, USA
    J Clin Hypertens (Greenwich) 15:270-8. 2013
    ..3%, 6.6%, and 2.0%, respectively. Nebivolol, added to an ACE inhibitor or ARB, provides additional blood pressure reduction with little or no effect on glucose metabolism in hypertensive patients with prediabetes...
  65. pmc Endogenous thrombospondin 1 protects the pressure-overloaded myocardium by modulating fibroblast phenotype and matrix metabolism
    Ying Xia
    Wilf Family Cardiovascular Research Institute, Department of Medicine, Division of Cardiology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    Hypertension 58:902-11. 2011
    ....
  66. pmc Activation of p300 histone acetyltransferase activity is an early endothelial response to laminar shear stress and is essential for stimulation of endothelial nitric-oxide synthase mRNA transcription
    Wei Chen
    Division of Cardiology, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30022, USA
    J Biol Chem 283:16293-8. 2008
    ..This was associated with opening of chromatin at the SSRE. In conclusion, these studies reveal a previously unknown role of p300/HAT activation as a very early response to shear that is essential for increasing eNOS mRNA levels...
  67. ncbi request reprint G protein-coupled receptor kinase function is essential for chemosensation in C. elegans
    Hana S Fukuto
    Department of Biology, University of California, San Diego, La Jolla, CA 92093 USA
    Neuron 42:581-93. 2004
    ..These results demonstrate that loss of GRK function can lead to reduced GPCR signal transduction and suggest an important role for RGS proteins in the regulation of chemosensation...
  68. ncbi request reprint Antitumor activities of TEM8-Fc: an engineered antibody-like molecule targeting tumor endothelial marker 8
    Hai Feng Duan
    Beijing Institute of Radiation Medicine, Haidian District, Beijing, China
    J Natl Cancer Inst 99:1551-5. 2007
    ..TEM8-Fc is a novel therapeutic antibody-like agent in the management of solid tumors that may act by trapping M2-PK...
  69. doi request reprint Protein expression and amplification of AIB1 in human urothelial carcinoma of the bladder and overexpression of AIB1 is a new independent prognostic marker of patient survival
    Jun Hang Luo
    The State Key Laboratory of Oncology in South China, Sun Yat sen University Cancer Center, Guangzhou, China
    Int J Cancer 122:2554-61. 2008
    ..Thus, these findings provide evidence that an overexpression of AIB1, as detected by immunohistochemistry, is an independent molecular marker for poor prognosis (shortened survival time) of patients with UC...
  70. ncbi request reprint Evidence of electroconformational changes in membrane proteins: field-induced reductions in intra membrane nonlinear charge movement currents
    Wei Chen
    Cellular and Molecular Biophysics, Department of Physics, University of South Florida, 4202 E Fowler Avenue, PHY 114 Tampa, FL 33620, USA
    Bioelectrochemistry 63:333-5. 2004
    ..The results indicate a possible mechanism involved in electrical injury: dysfunctions of the voltage-dependent membrane proteins caused by electroconformational damages in their voltage-sensors...
  71. ncbi request reprint [Relationship between epithelial-immunologic cells transdifferentiation and pseudoepitheliomatous granuloma lesion]
    Du yin Jiang
    Key Laboratory of Wound Repair, People s Liberation Army, 304th Hospital of People s Liberation Army, 100037 Beijing, China
    Zhonghua Wai Ke Za Zhi 42:400-5. 2004
    ..The correlation of abnormal transdifferentiation of epithelial cells to immunologic cells and the occurrence of PEG lesion was investigated...
  72. ncbi request reprint Resealing dynamics of a cell membrane after electroporation
    Martin Bier
    Department of Physics, East Carolina University, Greenville, NC 27858, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 66:062905. 2002
    ..We obtain a line tension of 3.6 x 10(-6) N, which is similar to that found in artificial lipid bilayers...
  73. ncbi request reprint Proteasome-mediated destruction of the cyclin a/cyclin-dependent kinase 2 complex suppresses tumor cell growth in vitro and in vivo
    Wei Chen
    Neuro Oncology Branch, National Cancer Institute, National Institutes of Neurological Disorder and Stroke, NIH, Bethesda, Maryland 20892, USA
    Cancer Res 64:3949-57. 2004
    ..These data demonstrate that cyclin A and/or the cyclin A/cdk2 complex is a promising anticancer target with a high therapeutic index...
  74. ncbi request reprint Formation of MacroH2A-containing senescence-associated heterochromatin foci and senescence driven by ASF1a and HIRA
    Rugang Zhang
    Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    Dev Cell 8:19-30. 2005
    ..These data indicate that HIRA and ASF1a drive formation of macroH2A-containing SAHF and senescence-associated cell cycle exit, via a pathway that appears to depend on flux of heterochromatic proteins through PML bodies...
  75. ncbi request reprint Comparative analysis of murine marrow-derived dendritic cells generated by Flt3L or GM-CSF/IL-4 and matured with immune stimulatory agents on the in vivo induction of antileukemia responses
    Brenda J Weigel
    University of Minnesota Cancer Center and Department of Pediatrics, Division of Pediatric Hematology Oncology and Blood and Marrow Transplant, Minneapolis 55455, USA
    Blood 100:4169-76. 2002
    ..These data have important clinical implications and are the first to show that Flt3L-generated DCs can provide antitumor protection and that nontoxic agents such as CpGs and Flt3L may be useful in the clinical development of DC vaccines...
  76. ncbi request reprint [Modification of Chinese hamster ovary cells]
    Da Zhi Lai
    Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China
    Sheng Wu Gong Cheng Xue Bao 18:415-9. 2002
    ..So in addition to optimizing CHO system in respect of medium, environment and expression vector, modification of CHO cells themselves has drawn more and more attention. Here the main progress in CHO-modification is reviewed...
  77. ncbi request reprint FLT3 ligand administration after hematopoietic cell transplantation increases circulating dendritic cell precursors that can be activated by CpG oligodeoxynucleotides to enhance T-cell and natural killer cell function
    Wei Chen
    University of Minnesota Cancer Center, Minneapolis, Minnesota 5455, USA
    Biol Blood Marrow Transplant 11:23-34. 2005
    ..These findings suggest that FLT3-L mobilization of DC precursors followed by a specific DC stimulus such as CpG ODN may provide a novel strategy to manipulate antitumor immunity in patients after HCT...
  78. ncbi request reprint Five Lec1 CHO cell mutants have distinct Mgat1 gene mutations that encode truncated N-acetylglucosaminyltransferase I
    Wei Chen
    Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, New York, NY 10461, USA
    Glycobiology 13:43-50. 2003
    ..These Lec1 mutants provide a novel cohort for investigating the effects on Golgi trafficking and kin recognition of deletion mutants of GlcNAc-TI expressed at endogenous rather than nonphysiological levels...
  79. pmc Thrombopoietin cooperates with FLT3-ligand in the generation of plasmacytoid dendritic cell precursors from human hematopoietic progenitors
    Wei Chen
    Cancer Center and Department of Pediatrics, Division of Hematology, Oncology, and Bone Marrow Transplantation, niversity of Minnesota, Minneapolis, MN 55455, USA
    Blood 103:2547-53. 2004
    ..This culture system, combined with the use of adult peripheral blood CD34+ HPCs purified from G-CSF-mobilized donors, permits the generation of more than 10(9) IPCs from a single blood donor...
  80. ncbi request reprint Plasma and cellular pharmacology of 8-chloro-adenosine in mice and rats
    Varsha Gandhi
    Department of Experimental Therapeutics, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA
    Cancer Chemother Pharmacol 50:85-94. 2002
    ..In addition, we sought to determine whether an inhibitor of adenosine deaminase, 2'-deoxycoformycin (dCF), could enhance intracellular formation of 8-Cl-ATP by preventing degradation of 8-Cl-Ado to 8-Cl-inosine (8-Cl-Ino)...
  81. ncbi request reprint [Characteristics of P38 mitogen-activated protein kinase and c-Jun expression in hypertrophic scar and their effects on scar formation]
    Wei Chen
    Key Research Laboratory of Wound Repair, 304th Hospital of PLA, Beijing, P R China 100037
    Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 17:379-81. 2003
    ..To observe the protein expression of phosphorylated form of P38 mitogen-activated protein kinase(P38MAPK) and c-Jun in hypertrophic scar skin and to explore their influences on the formation and maturation of hypertrophic scar...
  82. ncbi request reprint [Rapid construction of recombinant baculovirus carrying anti-HAV Fab gene using a baculovirus shuttle vector]
    Chang ming Yu
    Institute of Microbiology Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China
    Zhonghua Gan Zang Bing Za Zhi 11:510. 2003
  83. ncbi request reprint Molecular cloning, developmental expression, promoter analysis and functional characterization of the mouse CNBP gene
    Ken Shimizu
    Department of Cytokine Biology, The Forsyth Institute, Boston, MA 02115, USA
    Gene 307:51-62. 2003
    ..Our finds suggest that CNBP may play an important role in cell proliferation and tissue patterning during anterior-posterior axis, craniofacial and limb development by targeting c-Myc...
  84. pmc Virulence, immunogenicity, and protective efficacy of two recombinant Mycobacterium bovis bacillus Calmette-Guérin strains expressing the antigen ESAT-6 from Mycobacterium tuberculosis
    Lang Bao
    Research Unit of Infection and Immunity, West China Medical Center, Sichuan University, No 17, 3rd Section, Ren Min Nan Road, Chengdu, Sichuan 610041, People s Republic of China
    Infect Immun 71:1656-61. 2003
    ..Both rBCG-1 and rBCG-2 conferred marked protection against M. tuberculosis infection, yet in terms of protective efficacy, they showed no significant improvements upon conventional BCG vaccine...
  85. ncbi request reprint Review of current progress in the structure and function of Smad proteins
    Wei Chen
    PLA Key Laboratory of Wound Repair, 304th Hospital, Beijing 100037, China
    Chin Med J (Engl) 115:446-50. 2002
    ..To review the recent developments in the structure and function of Smad proteins...
  86. ncbi request reprint Supra-physiological membrane potential induced conformational changes in K+ channel conducting system of skeletal muscle fibers
    Wei Chen
    Center for Cellular and Molecular Biophysics, Department of Physics, The University of South Florida, 4202 E Fowler Ave, PHY 114, Tampa, FL 33620, USA
    Bioelectrochemistry 62:47-56. 2004
    ..These structural changes in membrane proteins, and therefore their dysfunctions, may be involved in the mechanisms underlying electrical injury...
  87. ncbi request reprint [Expression characteristics of transforming growth factor-beta1 in human skin at different development stages of gestation]
    Wei Chen
    Key Research Laboratory of Wound Repair, Burns Institute, 304 th Hospital of PLA, Beijing 100037, China
    Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 16:206-9. 2004
    ....
  88. ncbi request reprint [Change of gene expression of transforming growth factor-beta1, Smad 2 and Smad 3 in hypertrophic scars skins]
    Wei Chen
    PLA Key Research Laboratory of Wound Repair, 304th Hospital, Beijing 100037, China
    Zhonghua Wai Ke Za Zhi 40:17-9. 2002
    ..To explore the regulatory mechanisms of transforming growth factor-beta1 (TGF-beta1) and two transcriptional factors Smad 2, 3 on hypertrophic scar formation and fetal scarless healing...
  89. ncbi request reprint Identification and preliminary characterization of cell-wall-anchored proteins of Staphylococcus epidermidis
    M Gabriela Bowden
    Center for Extracellular Matrix Biology, Institute of Biosciences and Technology, Texas A and M University Health Science Center, Houston, TX 77030 3303, USA
    Microbiology 151:1453-64. 2005
    ..Western blot analyses of early-logarithmic and late-stationary in vitro cultures suggest that different regulatory mechanisms control the expression of the Ses proteins...
  90. ncbi request reprint Electrical activation of Na/K pumps can increase ionic concentration gradient and membrane resting potential
    Wei Chen
    Department of Cellular and Molecular Biophysics, University of South Florida, Tampa, Florida 33620, USA
    J Membr Biol 214:147-55. 2006
    ....
  91. ncbi request reprint A redox-dependent pathway for regulating class II HDACs and cardiac hypertrophy
    Tetsuro Ago
    Department of Cell Biology and Molecular Medicine, Cardiovascular Research Institute, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark, NJ 07103, USA
    Cell 133:978-93. 2008
    ..Our study reveals a novel regulatory mechanism of cardiac hypertrophy through which the nucleocytoplasmic shuttling of class II HDACs is modulated by their redox modification in a Trx1-sensitive manner...
  92. ncbi request reprint Transcriptional activation of hepatic ACSL3 and ACSL5 by oncostatin m reduces hypertriglyceridemia through enhanced beta-oxidation
    Yue Zhou
    VA Palo Alto Health Care System, 3801 Miranda Avenue, Palo Alto, CA 94304, USA
    Arterioscler Thromb Vasc Biol 27:2198-205. 2007
    ..Interestingly, the OM effect of lowering plasma TG was more pronounced than LDL-cholesterol reduction, suggesting additional LDLR-independent actions. Here, we investigated mechanisms underlying the direct TG-lowering effect of OM...
  93. ncbi request reprint Taspine downregulates VEGF expression and inhibits proliferation of vascular endothelial cells through PI3 kinase and MAP kinase signaling pathways
    Jing Zhao
    Center for Laboratory Medicine, The First Affiliated Hospital, Xi an Jiaotong University, Xi an 710061, PR China
    Biomed Pharmacother 62:383-9. 2008
    ..Taspine also significantly inhibited cell proliferation of HUVECs induced by VEGF165. HUVECs treated with taspine showed decreased Akt and Erk1/2 activities...
  94. pmc Monitoring receptor-ligand interactions between surfaces by thermal fluctuations
    Wei Chen
    Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332 0363, USA
    Biophys J 94:694-701. 2008
    ..This novel method provides a powerful tool to study cell adhesion and signaling mediated by single or multiple receptor-ligand species...
  95. doi request reprint Long-term efficacy of duloxetine in women with stress urinary incontinence
    Richard C Bump
    Lilly Research Laboratories, Indianapolis, IN 46285, USA
    BJU Int 102:214-8. 2008
    ....
  96. doi request reprint EAPF/Phafin-2, a novel endoplasmic reticulum-associated protein, facilitates TNF-alpha-triggered cellular apoptosis through endoplasmic reticulum-mitochondrial apoptotic pathway
    Changfei Li
    Institute of Immunology, Zhejiang University, Hangzhou 310031, People s Republic of China
    J Mol Med (Berl) 86:471-84. 2008
    ....
  97. ncbi request reprint Comparative effects of SPRM asoprisnil (J867) on proliferation, apoptosis, and the expression of growth factors in cultured uterine leiomyoma cells and normal myometrial cells
    Noriyuki Ohara
    Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe, Japan
    Reprod Sci 14:20-7. 2007
    ....
  98. ncbi request reprint Adaptor protein LAPF recruits phosphorylated p53 to lysosomes and triggers lysosomal destabilization in apoptosis
    Nan Li
    Institute of Immunology and National Key Laboratory of Medical Immunology, Second Military Medical University, Shanghai, P R China
    Cancer Res 67:11176-85. 2007
    ..The data suggest that phosphorylated p53 (Ser(15/18)) might translocate to lysosome via forming complexes with adaptor protein LAPF and subsequently result in LMP and apoptosis, which might be in a transcription-independent manner...
  99. ncbi request reprint SAGE library screening reveals ILT7 as a specific plasmacytoid dendritic cell marker that regulates type I IFN production
    Minkwon Cho
    Department of Immunobiology, Ginkgo Biomedical Research Institute, Tokyo, Japan
    Int Immunol 20:155-64. 2008
    ..We conclude that ILT7 is a key regulator of human pDC function...
  100. ncbi request reprint [The experimental studies on cell transplantation into chronic ischemic myocardium using mesenchymal stem cells modified by recombinant adenovirus carrying vascular endothelial growth factors 165 gene]
    Chang Qing Gao
    Department of Cardiovascular Surgery, General Hospital of People s Liberation Army, Beijing 100853, China
    Zhonghua Wai Ke Za Zhi 45:990-3. 2007
    ....
  101. ncbi request reprint Biological cross-talk between WNK1 and the transforming growth factor beta-Smad signaling pathway
    Byung Hoon Lee
    Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390 9041, USA
    J Biol Chem 282:17985-96. 2007
    ..In addition, TGFbeta-induced target gene transcripts were increased in WNK1 small interfering RNA cells. These findings suggest WNK1 as a dual modulator of TGFbeta-Smad signaling pathways...

Research Grants3

  1. Innovative Assays for Oncogenic Hedgehog Signaling
    Wei Chen; Fiscal Year: 2007
    ..In particular it should be possible to identify critical proteins modulating altered signaling pathways in pancreatic adenocarcinoma and design pharmacologic strategies to target them for therapeutic benefit. ..
  2. Innovative Assays for Oncogenic Hedgehog Signaling
    Wei Chen; Fiscal Year: 2006
    ..In particular it should be possible to identify critical proteins modulating altered signaling pathways in pancreatic adenocarcinoma and design pharmacologic strategies to target them for therapeutic benefit. ..